Journal of Microbiology

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Analysis of a Novel Class 1 Integron Containing Metallo-β-Lactamase Gene VIM-2 in Pseudomonas aeruginosa: Jae Hoon Jeong , Kyeong Seob Shin , Jang Won Lee , Eun Jin Park , Seung-Yeol Son; J. Microbiol. 2009;47(6):753-759. Published online February 4, 2010; DOI: https://doi.org/10.1007/s12275-008-0272-2

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Carbapenems such as imipenem are stable to most β-lactamases. Recently, increased numbers of carbapenemase producing Gram-negative bacterial strains have been isolated because of the increased use of cabapenems. In this respect, control of these infectious carbapenemase producing Gram-negative bacteria and understanding their resistance mechanism are becoming more important. These carbapenem-hydrolyzing β-lactamase genes have been reported to exist mostly as gene cassettes in an integron. This implies that antibiotic resistance genes may be transferred to other bacteria via the integron. In the present study, we identified and analyzed an integron containing VIM-2 type metallo-β-lactamase gene in a carbapenemase producing Pseudomonas aeruginosa. In addition, the possibility of resistance spread by integron located in a plasmid was tested. Among glucose non-fermenting Gram-negative bacilli with reduced imipenem susceptibility (MIC≥8 μg/ml) isolated from Korean patients, P. aeruginosa 1082 showed resistance to most β-lactams, cephalosporin, and aminoglycoside. We found that P. aeruginosa 1082 was inhibited by EDTA in EDTA double disk synergy test which means that this strain produces metallo-β-lactamase. Class 1 integron containing blaVIM-2 (carbapenem resistance gene), qacF (quaternary ammonium compound resistance gene), aacA4 (aminoglycoside resistance gene), catB3 (chloramphenicol resistance gene), blaOXA-30 (extended-spectrum β-lactam resistance gene), and aadA1 (aminoglycoside resistance gene) gene cassettes was detected in P. aeruginosa 1082. The size of the integron was 5,246 bp and the structure and arrangement of the integron was a novel one in comparison with other integrons found in other P. aeruginosa. The integron could be transferred to Escherichia coli JM109 from P. aeruginosa 1082 possibly via self-transferable plasmid DNA. The integron and a blaVIM-2 gene were detected in the plasmid DNA of the transconjugants whose imipenem resistance was slightly increased as a result of accepting the integron from the donor strain.

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NOTE] Nomenclature of ISCR1 Elements Capable of Mobilizing Antibiotic Resistance Genes Present in Complex Class 1 Integrons: Seung Ghyu Sohn , Jae Jin Lee , Jae Seok Song , Jung Hun Lee , Ha Ik Sun , Kwang Seung Park , Il Kwon Bae , Jung-Hyun Lee , Byeong Chul Jeong , Sang Hee Lee; J. Microbiol. 2009;47(4):514-516. Published online September 9, 2009; DOI: https://doi.org/10.1007/s12275-009-0054-5

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Abstract

The dissemination of many antibiotic resistance genes has arisen among members of the family Enterobacteriaceae. The dissemination mechanism of these antibiotic resistance genes is closely linked with insertion sequence common region 1 (ISCR1). Thus, caution must be taken in clinical settings to prevent further dissemination of these antibiotic resistance genes. A nomenclature system of ISCR1 variants, important for the antibiotic resistance dissemination, was proposed. The proposed system can designate all ISCR1 variants on the basis of the detection time and by considering amino-acid substitution(s) compared with ISCR1a. This nomenclature system of ISCR1 variants can be applied to 19 groups (ISCR1 to ISCR19) of the ISCR family and help some researchers to correctly designate new ISCR subgroups.

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Detection of Pseudomonas aeruginosa Carried a New Array of Gene Cassettes within Class 1 Integron Isolated from a Teaching Hospital in Nanjing, China: Yuan Wu† , Hui Li† , Jun Li , Zu Hu Huang; J. Microbiol. 2008;46(6):687-691. Published online December 24, 2008; DOI: https://doi.org/10.1007/s12275-008-0021-6

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Abstract

We report here novel array of gene cassettes found in single variable region of class 1 integron disseminated in Pseudomonas aeruginosa isolated from a teaching hospital in Nanjing, Jiangsu Province, China. 29 of 47 (61%) P. aeruginosa strains were confirmed haboured class 1 integron, and all the positive strains have the same variable region confirmed by PCR and RFLP methods. The variable region contained an unreported order of four gene cassettes aac(6’)-II-aadA13-cmlA8-oxa 10. Of those, cmlA8 gene was a variant of cmlA5 encoding non-enzymatic protein which putatively confer resistance to chloramphenicol. Susceptibility testing revealed multidrug-resistant mechanisms were involved in the class 1 integron positive clinical isolates. And the class 1 integron located on an about 15 kb transferable plasmid was certified by conjugation experiment and plasmid DNA analysis. The macro restriction profile indicated those clinical strains were clonally related.

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Distribution and Molecular Characterization of Resistance Gene Cassettes Containing Class 1 Integrons in Multi-Drug Resistant (MDR) Clinical Isolates of Pseudomonas aeruginosa

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Prevalence and molecular characterization of class 1 integrons among clinical isolates of Pseudomonas aeruginosa in Northwest of Iran
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Ke-Yu Hsiao, Mei-Feng Lee, Chien-Fang Peng
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Analysis of integrons and associated gene cassettes in clinical isolates of multidrug resistant Pseudomonas aeruginosa from Southwest Nigeria
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Molecular Characterization of Antibiotic Resistant Escherichia coli Strains Isolated from Tap and Spring Waters in a Coastal Region in Turkey: Osman Birol Ozgumus , Elif Celik-Sevim , Sengul Alpay-Karaoglu , Cemal Sandalli , Ali Sevim; J. Microbiol. 2007;45(5):379-387.; DOI: https://doi.org/2600 [pii]

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Abstract: A hundred and seventeen antibiotic-resistant Escherichia coli strains were isolated from public tap and spring waters which were polluted by fecal coliforms. There were no significant differences between two water sources as to the coliform pollution level (p> 0.05). All E. coli isolates were detected to be resistant to one or more antibiotics tested. Nearly 42% of the isolates showed multiresistant phenotype. Three (2.5%) of these isolates contained class 1 integron. Sequencing analysis of variable regions of the class 1 integrons showed two gene cassette arrays, dfr1-aadA1 and dhfrA17-aadA5. Resistance to ampicillin, tetracycline or trimethoprim-sulfamethoxazole was transferable according to the results of conjugation experiments. The rate of tetracycline resistance was 15%. tet(A)-mediated tetracycline resistance was widespread among tetracycline-resistant E. coli isolates. Genotyping by BOX-polymerase chain reaction (BOX-PCR) showed that some of the strains were epidemiologically related. This is the first report on the prevalence and characterization of class 1 integron-containing E. coli isolates of environmental origin in Turkey.

Published Erratum

Erratum: Secretions from Serratia marcescens Inhibit the Growth and Biofilm Formation of Candida spp. and Cryptococcus neoformans: Caiyan Xin , Fen Wang , Jinping Zhang , Quan Zhou , Fangyan Liu , Chunling Zhao , Zhangyong Song; J. Microbiol. 2023;61(4):479-479.; DOI: https://doi.org/10.1007/s12275-023-00037-5

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Abstract

Erratum: Journal of Microbiology (2023) 61:221–232 https://doi.org/10.1007/s12275-022-00007-3 In this article the acknowledgment has been given erroneously. It should be read as follows: This research was supported financially by the Sichuan Science and Technology Program (2023NSFSC1698, 2023NSFSC0529, 2022NSFSC1539, and 2022YFS0629) and Luzhou (2021-JYJ-73 and 2022-JYJ-159), the Technology Strategic Cooperation Project of Luzhou Municipal People’s Government Southwest Medical University (2020LZXNYDJ38 and 2020LZXNYDJ23), and the Foundation of Southwest Medical University (2021ZKMS008, 2022QN042, 2022QN085, 2022QN102, and 2022QN118). The original article has been corrected.

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