A novel gene (bgl) encoding a cold-adapted β-glucosidase
was cloned from the marine bacterium Alteromonas sp.
L82. Based on sequence analysis and its putative catalytic
conserved region, Bgl belonged to the glycoside hydrolase
family 1. Bgl was overexpressed in E. coli and purified by
Ni2+ affinity chromatography. The purified recombinant β-
glucosidase showed maximum activity at temperatures between
25°C to 45°C and over the pH range 6 to 8. The enzyme
lost activity quickly after incubation at 40°C. Therefore,
recombinant β-glucosidase appears to be a cold-adapted
enzyme. The addition of reducing agent doubled its activity
and 2 M NaCl did not influence its activity. Recombinant
β-glucosidase was also tolerant of 700 mM glucose and some
organic solvents. Bgl had a Km of 0.55 mM, a Vmax of 83.6
U/mg, a kcat of 74.3 s-1 and kcat/Km of 135.1 at 40°C, pH 7 with
4-nitrophenyl-β-D-glucopyranoside as a substrate. These
properties indicate Bgl may be an interesting candidate for
biotechnological and industrial applications.
Citations
Citations to this article as recorded by
Streptomyces beigongshangae sp. nov., isolated from baijiu fermented grains, could transform ginsenosides of Panax notoginseng
Bo Liu, Haoyue Gu, Rui Shi, Xiahong He, Zhanbin Sun, Qing Ren, Hanxu Pan
International Journal of Systematic and Evolutionary Microbiology
.2024;[Epub] CrossRef
A novel GH1 β-glucosidase from an Arctic bacterium: Characterization and secretory expression in Bacillus subtilis Jingjing Sun, Wei Wang, Jianhua Hao Process Biochemistry.2024; 140: 108. CrossRef
Screening, cloning, immobilization and application prospects of a novel β-glucosidase from the soil metagenome Qian Yao, Jin Xu, Nan Tang, Weiji Chen, Quliang Gu, He Li Environmental Research.2024; 244: 117676. CrossRef
Characterization of a novel cold-adapted GH1 β-glucosidase from Psychrobacillus glaciei and its application in the hydrolysis of soybean isoflavone glycosides Jinjian He, Jiajing Duan, Pinglian Yu, Yuying Li, Mansheng Wang, Xiu Zhang, Zishu Chen, Pengjun Shi Current Research in Food Science.2024; 8: 100777. CrossRef
Biochemical and in silico structural properties of a thermo-acid stable β-glucosidase from Beauveria bassiana Buka Magwaza, Ayodeji Amobonye, Prashant Bhagwat, Santhosh Pillai Heliyon.2024; 10(7): e28667. CrossRef
Moderately thermostable GH1 β-glucosidases from hyperacidophilic archaeon Cuniculiplasma divulgatum S5 Anna N Khusnutdinova, Hai Tran, Saloni Devlekar, Marco A Distaso, Ilya V Kublanov, Tatiana Skarina, Peter Stogios, Alexei Savchenko, Manuel Ferrer, Olga V Golyshina, Alexander F Yakunin, Peter N Golyshin FEMS Microbiology Ecology.2024;[Epub] CrossRef
Structural determinants of cold activity and glucose tolerance of a family 1 glycoside hydrolase (GH1) from Antarctic Marinomonas sp. ef1 Louise Jane Gourlay, Marco Mangiagalli, Elisabetta Moroni, Marina Lotti, Marco Nardini The FEBS Journal.2024; 291(13): 2897. CrossRef
Partial characterization of β-glucosidase, β-xylosidase, and α-l-arabinofuranosidase from Jiangella alba DSM 45237 and their potential in lignocellulose-based biorefining Zeynep Gül Aytaş, Münir Tunçer, Çağrı Seda Kul, Sümeyye Cilmeli, Nurayan Aydın, Tuğrul Doruk, Ali Osman Adıgüzel Sustainable Chemistry and Pharmacy.2023; 31: 100900. CrossRef
Heterologous expression and characterization of salt-tolerant β-glucosidase from xerophilic Aspergillus chevalieri for hydrolysis of marine biomass Hironori Senba, Daisuke Saito, Yukihiro Kimura, Shinichi Tanaka, Mikiharu Doi, Shinji Takenaka Archives of Microbiology.2023;[Epub] CrossRef
Expression of β-Glucosidases from the Yak Rumen in Lactic Acid Bacteria: A Genetic Engineering Approach Chuan Wang, Yuze Yang, Chunjuan Ma, Yongjie Sunkang, Shaoqing Tang, Zhao Zhang, Xuerui Wan, Yaqin Wei Microorganisms.2023; 11(6): 1387. CrossRef
Structural and functional insights of a cold-adaptive β-glucosidase with very high glucose tolerance from Microbacterium sp. CIAB417 Anjali Purohit, Lata Pawar, Sudesh Kumar Yadav Enzyme and Microbial Technology.2023; 169: 110284. CrossRef
Advances in cold-adapted enzymes derived from microorganisms Yehui Liu, Na Zhang, Jie Ma, Yuqi Zhou, Qiang Wei, Chunjie Tian, Yi Fang, Rongzhen Zhong, Guang Chen, Sitong Zhang Frontiers in Microbiology.2023;[Epub] CrossRef
Improving the catalytic activity of β-glucosidase from Coniophora puteana via semi-rational design for efficient biomass cellulose degradation Hai-Yan Zhou, Qi Chen, Yi-Feng Zhang, Dou-Dou Chen, Xiao-Nan Yi, De-Shui Chen, Xin-Ping Cheng, Mian Li, Hong-Yan Wang, Kai-Qian Chen, Zhi-Qiang Liu, Yu-Guo Zheng Enzyme and Microbial Technology.2023; 164: 110188. CrossRef
Study on the Biochemical Characterization and Selectivity of Three β-Glucosidases From Bifidobacterium adolescentis ATCC15703 Yanbo Hu, Liyuan Zhai, Huili Hong, Zenghui Shi, Jun Zhao, Duo Liu Frontiers in Microbiology.2022;[Epub] CrossRef
Biochemical characterization of a novel glucose-tolerant GH3 β-glucosidase (Bgl1973) from Leifsonia sp. ZF2019 Yi He, Chenxi Wang, Ronghu Jiao, Qinxue Ni, Yan Wang, Qianxin Gao, Youzuo Zhang, Guangzhi Xu Applied Microbiology and Biotechnology.2022; 106(13-16): 5063. CrossRef
Spatial variability of bacterial community compositions in the Mariana Trench Wei Wang, Jingjing Sun, Jianhua Hao Canadian Journal of Microbiology.2022; 68(10): 633. CrossRef
Life from a Snowflake: Diversity and Adaptation of Cold-Loving Bacteria among Ice Crystals Carmen Rizzo, Angelina Lo Giudice Crystals.2022; 12(3): 312. CrossRef
Cold-Active β-Galactosidases: Insight into Cold Adaptation Mechanisms and Biotechnological Exploitation Marco Mangiagalli, Marina Lotti Marine Drugs.2021; 19(1): 43. CrossRef
Two Key Amino Acids Variant of α-l-arabinofuranosidase from Bacillus subtilis Str. 168 with Altered Activity for Selective Conversion Ginsenoside Rc to Rd Ru Zhang, Shi Quan Tan, Bian Ling Zhang, Zi Yu Guo, Liang Yu Tian, Pei Weng, Zhi Yong Luo Molecules.2021; 26(6): 1733. CrossRef
Cloning, expression, biochemical characterization, and molecular docking studies of a novel glucose tolerant β-glucosidase from Saccharomonospora sp. NB11 Numan Saleh Zada, Ali Osman Belduz, Halil Ibrahim Güler, Anum Khan, Miray Sahinkaya, Arife Kaçıran, Hilal Ay, Malik Badshah, Aamer Ali Shah, Samiullah Khan Enzyme and Microbial Technology.2021; 148: 109799. CrossRef
A novel β-glucosidase from a hot-spring metagenome shows elevated thermal stability and tolerance to glucose and ethanol Girija Kaushal, Amit K. Rai, Sudhir P. Singh Enzyme and Microbial Technology.2021; 145: 109764. CrossRef
Homology analysis of 35 β-glucosidases in Oenococcus oeni and biochemical characterization of a novel β-glucosidase BGL0224 Jie Zhang, Ning Zhao, Junnan Xu, Yiman Qi, Xinyuan Wei, Mingtao Fan Food Chemistry.2021; 334: 127593. CrossRef
A glucose tolerant β-glucosidase from Thermomicrobium roseum that can hydrolyze biomass in seawater Sushant K. Sinha, Maithili Datta, Supratim Datta Green Chemistry.2021; 23(18): 7299. CrossRef
An overview on marine cellulolytic enzymes and their potential applications Noora Barzkar, Muhammad Sohail Applied Microbiology and Biotechnology.2020; 104(16): 6873. CrossRef
A Novel Glucose-Tolerant GH1 β-Glucosidase and Improvement of Its Glucose Tolerance Using Site-Directed Mutation Jingjing Sun, Wei Wang, Yu Ying, Jianhua Hao Applied Biochemistry and Biotechnology.2020; 192(3): 999. CrossRef
Magnetically recyclable catalytic nanoparticles grafted with Bacillus subtilis β-glucosidase for efficient cellobiose hydrolysis Shivangi Chamoli, Ekta Yadav, Hemansi, Jitendra Kumar Saini, Ashok Kumar Verma, Naveen Kumar Navani, Piyush Kumar International Journal of Biological Macromolecules.2020; 164: 1729. CrossRef
Engineering of β-Glucosidase Bgl15 with Simultaneously Enhanced Glucose Tolerance and Thermostability To Improve Its Performance in High-Solid Cellulose Hydrolysis Lichuang Cao, Ran Chen, Xin Huang, Shuifeng Li, Sufang Zhang, Xiangpeng Yang, Zongmin Qin, Wei Kong, Wei Xie, Yuhuan Liu Journal of Agricultural and Food Chemistry.2020; 68(19): 5391. CrossRef
A d-glucose- and d-xylose-tolerant GH1 β-glucosidase from Cellulosimicrobium funkei HY-13, a fibrolytic gut bacterium of Eisenia fetida Do Young Kim, Jonghoon Kim, Sun Hwa Lee, Chungwook Chung, Dong-Ha Shin, Bon-Hwan Ku, Kwang-Hee Son, Ho-Yong Park Process Biochemistry.2020; 94: 282. CrossRef
A rationally identified marine GH1 β‐glucosidase has distinguishing functional features for simultaneous saccharification and fermentation Amanda S. de Sousa, Ricardo R. de Melo, Renan Y. Miyamoto, Mariana A. B. Morais, Liliane P. Andrade, Natália Milan, Mayara C. de Avila, Cláudia M. de Souza, Regina C. Adão, Josiane A. Scarpassa, Plínio S. Vieira, Leandro V. dos Santos, Carlos H. I. Ramos, Biofuels, Bioproducts and Biorefining.2020; 14(6): 1163. CrossRef
Deep Hypersaline Anoxic Basins as Untapped Reservoir of Polyextremophilic Prokaryotes of Biotechnological Interest Stefano Varrella, Michael Tangherlini, Cinzia Corinaldesi Marine Drugs.2020; 18(2): 91. CrossRef
Aroma enhancement of instant green tea infusion using β-glucosidase and β-xylosidase Ting Zhang, Ke Fang, Hui Ni, Ting Li, Li Jun Li, Qing Biao Li, Feng Chen Food Chemistry.2020; 315: 126287. CrossRef
RNase G controls tpiA mRNA abundance in response to oxygen availability in Escherichia coli Jaejin Lee, Dong-Ho Lee, Che Ok Jeon, Kangseok Lee Journal of Microbiology.2019; 57(10): 910. CrossRef
Comparison between irradiating and autoclaving citrus wastes as substrate for solid‐state fermentation by
Aspergillus aculeatus
H. Ni, T. Zhang, X. Guo, Y. Hu, A. Xiao, Z. Jiang, L. Li, Q. Li Letters in Applied Microbiology.2019;[Epub] CrossRef
The coordinated action of RNase III and RNase G controls enolase expression in response to oxygen availability in Escherichia coli Minho Lee, Minju Joo, Minji Sim, Se-Hoon Sim, Hyun-Lee Kim, Jaejin Lee, Minkyung Ryu, Ji-Hyun Yeom, Yoonsoo Hahn, Nam-Chul Ha, Jang-Cheon Cho, Kangseok Lee Scientific Reports.2019;[Epub] CrossRef
Identification and molecular characterization of a psychrophilic GH1 β-glucosidase from the subtropical soil microorganism Exiguobacterium sp. GXG2 Bangqiao Yin, Hengsen Gu, Xueyan Mo, Yue Xu, Bing Yan, Quanwen Li, Qian Ou, Bo Wu, Chen Guo, Chengjian Jiang AMB Express.2019;[Epub] CrossRef