Journal of Microbiology

Journal Articles

Identification of trehalose as a compatible solute in different species of acidophilic bacteria: Pedro A. Galleguillos , Barry M. Grail , Kevin B. Hallberg , Cecilia S. Demergasso , D. Barrie Johnson; J. Microbiol. 2018;56(10):727-733. Published online September 28, 2018; DOI: https://doi.org/10.1007/s12275-018-8176-2

76 View
0 Download
21 Crossref

The major industrial heap bioleaching processes are located in desert regions (mainly Chile and Australia) where fresh water is scarce and the use of resources with low water activity becomes an attractive alternative. However, in spite of the importance of the microbial populations involved in these processes, little is known about their response or adaptation to osmotic stress. In order to investigate the response to osmotic stress in these microorganisms, six species of acidophilic bacteria were grown at elevated osmotic strength in liquid media, and the compatible solutes synthesised were identified using ion chromatography and MALDI-TOF mass spectrometry. Trehalose was identified as one of, or the sole, compatible solute in all species and strains, apart from Acidithiobacillus thiooxidans where glucose and proline levels increased at elevated osmotic potentials. Several other potential compatible solutes were tentatively identified by MALDITOF analysis. The same compatible solutes were produced by these bacteria regardless of the salt used to produce the osmotic stress. The results correlate with data from sequenced genomes which confirm that many chemolithotrophic and heterotrophic acidophiles possess genes for trehalose synthesis. This is the first report to identify and quantify compatible solutes in acidophilic bacteria that have important roles in biomining technologies.

Citations

Citations to this article as recorded by

Adaptive response of the holdase chaperone network of Acidithiobacillus ferrooxidans ATCC 23270 to stresses and energy sources
Katherin Izquierdo-Fiallo, Claudia Muñoz-Villagrán, Christian Schimpf, Mick Parra Mardonez, David Rafaja, Michael Schlömann, Mario Tello, Omar Orellana, Gloria Levicán
World Journal of Microbiology and Biotechnology.2025;[Epub] CrossRef
Deciphering hypersaline tolerance mechanism of the pyridine-degrading strain Rhodococcus sp. PD10
Yangfan Hu, Zhichun Wang, Zizhen Liu, Korakot Sombatmankhong, Bo Yu
Environmental Technology & Innovation.2025; 38: 104168. CrossRef
Improvement in Salt Tolerance Ability of Pseudomonas putida KT2440
Min Fan, Shuyu Tan, Wei Wang, Xuehong Zhang
Biology.2024; 13(6): 404. CrossRef
A novel strategy for Klebsiella sp. to resist high salt and high phenol environmental stress
Jiejing Zhang, Chong Hu, Yu Wu, Jing Liang, Cesar Danilo Valle Exposito, Jianfeng Zhang
Process Biochemistry.2024; 146: 316. CrossRef
Multi-stress adaptive lifestyle of acidophiles enhances their robustness for biotechnological and environmental applications
Godwin O. Aliyu, Flora N. Ezugworie, Chukwudi O. Onwosi, Chukwudi I. Nnamchi, Chito C. Ekwealor, Victor C. Igbokwe, Rajesh K. Sani
Science of The Total Environment.2024; 954: 176190. CrossRef
Osmotic response in Leptospirillum ferriphilum isolated from an industrial copper bioleaching environment to sulfate
Dayana Arias, Víctor Zepeda, Ivan Nancucheo, Manuel Saldaña, Pedro A. Galleguillos
Frontiers in Microbiology.2024;[Epub] CrossRef
Interplay between desiccation and oxidative stress responses in iron-oxidizing acidophilic bacteria
Muñoz-Villagrán Claudia, Acevedo-Arbunic Javiera, Navarro-Salazar Sebastián, Fuentes-Rubio José, Levicán Gloria
Journal of Biotechnology.2024; 383: 64. CrossRef
Study on the intracellular adaptative mechanism of Acidithiobacillus caldus MTH-04 to NaCl stress
Min Li, Jianping Wen
Microbial Cell Factories.2023;[Epub] CrossRef
New Features of Acidophilic Bacteria of the Genus Sulfobacillus: Polysaccharide Biosynthesis and Degradation Pathways
Anna Panyushkina, Maxim Muravyov
Minerals.2023; 13(2): 255. CrossRef
Salinity changes the nitrification activity and community composition of comammox Nitrospira in intertidal sediments of Yangtze River estuary
Ran Jiang, Wen-Jing Qin, Ru-Yi Zhang, Kai Zhang, Xing Huang, Yong Li, Chen-Hao Zhou, Ting Zhu, Yan Zhang, Bin Zou, Ming Nie, Sung-Keun Rhee, Zhe-Xue Quan, Nick Bouskill
mSystems.2023;[Epub] CrossRef
Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2017–2018
David J. Harvey
Mass Spectrometry Reviews.2023; 42(1): 227. CrossRef
Eurypsychrophilic acidophiles: From (meta)genomes to low-temperature biotechnologies
Mark Dopson, Carolina González-Rosales, David S. Holmes, Nadia Mykytczuk
Frontiers in Microbiology.2023;[Epub] CrossRef
Reduction of greenhouse gas (GHG) emission in the vegetation-activated sludge process (V-ASP) involving decontaminated plants for decentralized wastewater treatment
Jian-Jiang Lu, Zi-Jun Dong, Pu Li, Wei-Jia Yan, Jia-Jia Yuan, Wen-Yi Dong, Fei-Yun Sun, Yun-Xian Shao
Journal of Cleaner Production.2022; 362: 132341. CrossRef
Identification of novel halophilic/halotolerant bacterial species producing compatible solutes
Furkan Orhan, Ertuğrul Ceyran
International Microbiology.2022; 26(2): 219. CrossRef
Transcriptomic analysis of chloride tolerance in Leptospirillum ferriphilum DSM 14647 adapted to NaCl
Javier Rivera-Araya, Thomas Heine, Renato Chávez, Michael Schlömann, Gloria Levicán, Benjamin J. Koestler
PLOS ONE.2022; 17(4): e0267316. CrossRef
Glutathione Synthetase Overexpression in Acidithiobacillus ferrooxidans Improves Halotolerance of Iron Oxidation
Yuta Inaba, Alan C. West, Scott Banta, Robert M. Kelly
Applied and Environmental Microbiology.2021;[Epub] CrossRef
Examining the Osmotic Response of Acidihalobacter aeolianus after Exposure to Salt Stress
Melissa K. Corbett, Liam Anstiss, April Gifford, Ross M. Graham, Elizabeth L. J. Watkin
Microorganisms.2021; 10(1): 22. CrossRef
From Laboratory towards Industrial Operation: Biomarkers for Acidophilic Metabolic Activity in Bioleaching Systems
Sabrina Marín, Mayra Cortés, Mauricio Acosta, Karla Delgado, Camila Escuti, Diego Ayma, Cecilia Demergasso
Genes.2021; 12(4): 474. CrossRef
Mechanisms of NaCl-tolerance in acidophilic iron-oxidizing bacteria and archaea: Comparative genomic predictions and insights
Javier Rivera-Araya, Ngoc Dieu Huynh, Marek Kaszuba, Renato Chávez, Michael Schlömann, Gloria Levicán
Hydrometallurgy.2020; 194: 105334. CrossRef
Osmotic Imbalance, Cytoplasm Acidification and Oxidative Stress Induction Support the High Toxicity of Chloride in Acidophilic Bacteria
Javier Rivera-Araya, Andre Pollender, Dieu Huynh, Michael Schlömann, Renato Chávez, Gloria Levicán
Frontiers in Microbiology.2019;[Epub] CrossRef
Uncovering the Mechanisms of Halotolerance in the Extremely Acidophilic Members of the Acidihalobacter Genus Through Comparative Genome Analysis
Himel N. Khaleque, Carolina González, Raihan Shafique, Anna H. Kaksonen, David S. Holmes, Elizabeth L. J. Watkin
Frontiers in Microbiology.2019;[Epub] CrossRef

Biosynthesis and uptake of glycine betaine as cold-stress response to low temperature in fish pathogen Vibrio anguillarum: Yue Ma , Qiyao Wang , Xiating Gao , Yuanxing Zhang; J. Microbiol. 2017;55(1):44-55. Published online December 30, 2016; DOI: https://doi.org/10.1007/s12275-017-6370-2

69 View
0 Download
22 Crossref

Abstract

Fish pathogen Vibrio anguillarum, a mesophile bacterium, is usually found in estuarine and marine coastal ecosystems worldwide that pose a constant stress to local organism by its fluctuation in salinity as well as notable temperature change. Though V. anguillarum is able to proliferate while maintain its pathogenicity under low temperature (5–18°C), so far, coldadaption molecular mechanism of the bacteria is unknown. In this study, V. anguillarum was found possessing a putative glycine betaine synthesis system, which is encoded by betABI and synthesizes glycine betaine from its precursor choline. Furthermore, significant up-regulation of the bet gene at the transcriptional level was noted in log phase in response to cold-stress. Moreover, the accumulation of betaine glycine was only found appearing at low growth temperatures, suggesting that response regulation of both synthesis system and transporter system are cold-dependent. Furthermore, in-frame deletion mutation in the two putative ABC transporters and three putative BCCT family transporters associated with glycine betaine uptake could not block cellular accumulation of betaine glycine in V. anguillarum under coldstress, suggesting the redundant feature in V. anguillarum betaine transporter system. These findings confirmed that glycine betaine serves as an effective cold stress protectant and highlighted an underappreciated facet of the acclimatization of V. anguillarum to cold environments.

Citations

Citations to this article as recorded by

Investigation of different cold adaptation abilities in Salmonella enterica serotype Typhimurium strains using extracellular metabolomic approach
Heesang Hong, Hye-Jin Kim, Hyun-Jun Kim, Cheorun Jo
International Microbiology.2024;[Epub] CrossRef
Study on the estradiol degradation gene expression and resistance mechanism of Rhodococcus R-001 under low-temperature stress
Qing Qiu, Han Li, Xuejian Sun, Lili Zhang, Kejian Tian, Menghan Chang, Shuaiguo Li, Dandan Zhou, Hongliang Huo
Chemosphere.2024; 358: 142146. CrossRef
Seasonal Mortality of Wild Atlantic Menhaden (Brevoortia tyrannus) Is Caused by a Virulent Clone of Vibrio (Listonella) anguillarum; Implications for Biosecurity along the Atlantic Coastal United States
Jan Lovy, Luke R. Iwanowicz, Timothy J. Welch, Bassem Allam, Rodman G. Getchell, Sabrina Geraci-Yee, Chris Good, Jeremy Snyder, Clayton D. Raines, Nilanjana Das, Zongfu Wu
Transboundary and Emerging Diseases.2024; 2024: 1. CrossRef
Surveillance de la photobactériose chez la daurade (Sparus aurata) et le bar (Dicentrarchus labrax) élevés en Tunisie
Kaouthar Maatouk, Sami Zaafrane, Hedia Attia El Hili
Revue d’élevage et de médecine vétérinaire des pays tropicaux.2023; 76: 1. CrossRef
A general overview of the multifactorial adaptation to cold: biochemical mechanisms and strategies
Ana Ramón, Adriana Esteves, Carolina Villadóniga, Cora Chalar, Susana Castro-Sowinski
Brazilian Journal of Microbiology.2023; 54(3): 2259. CrossRef
Shift of Choline/Betaine Pathway in Recombinant Pseudomonas for Cobalamin Biosynthesis and Abiotic Stress Protection
Larissa Balabanova, Iuliia Pentekhina, Olga Nedashkovskaya, Anton Degtyarenko, Valeria Grigorchuk, Yulia Yugay, Elena Vasyutkina, Olesya Kudinova, Aleksandra Seitkalieva, Lubov Slepchenko, Oksana Son, Liudmila Tekutyeva, Yury Shkryl
International Journal of Molecular Sciences.2022; 23(22): 13934. CrossRef
Light response of Vibrio parahaemolyticus
Yunato Kuroyanagi, Jiro Tsuchiya, Chunqi Jiang, Sayaka Mino, Hisae Kasai, Daisuke Motooka, Tetsuya Iida, Masataka Satomi, Tomoo Sawabe
Frontiers in Marine Science.2022;[Epub] CrossRef
Effect of sunlight and salinity on the survival of pathogenic and non‐pathogenic strains of Vibrio parahaemolyticus in water microcosms
Zaafrane Sami, Maatouk Kaouthar, Cherif Nadia, Ben Mansour Hedi
Water Environment Research.2022;[Epub] CrossRef
Stressed out: Bacterial response to high salinity using compatible solute biosynthesis and uptake systems, lessons from Vibrionaceae
Gwendolyn J. Gregory, E. Fidelma Boyd
Computational and Structural Biotechnology Journal.2021; 19: 1014. CrossRef
Molecular and Physiological Adaptations to Low Temperature in Thioalkalivibrio Strains Isolated from Soda Lakes with Different Temperature Regimes
Anne-Catherine Ahn, Evelien Jongepier, J. Merijn Schuurmans, W. Irene C. Rijpstra, Jaap S. Sinninghe Damsté, Erwin A. Galinski, Pawel Roman, Dimitry Sorokin, Gerard Muyzer, Rachel Mackelprang
mSystems.2021;[Epub] CrossRef
The Osmolyte Ties That Bind: Genomic Insights Into Synthesis and Breakdown of Organic Osmolytes in Marine Microbes
Erin L. McParland, Harriet Alexander, Winifred M. Johnson
Frontiers in Marine Science.2021;[Epub] CrossRef
The Temperature-Dependent Expression of the High-Pathogenicity Island Encoding Piscibactin in Vibrionaceae Results From the Combined Effect of the AraC-Like Transcriptional Activator PbtA and Regulatory Factors From the Recipient Genome
Marta A. Lages, Manuel L. Lemos, Miguel Balado
Frontiers in Microbiology.2021;[Epub] CrossRef
Improvement of a dry formulation of Pseudomonas protegens SN15-2 against Ralstonia solanacearum by combination of hyperosmotic cultivation with fluidized-bed drying
Xiaobing Wang, Danyan Tang, Wei Wang
BioControl.2020; 65(6): 751. CrossRef
Choline–betaine pathway contributes to hyperosmotic stress and subsequent lethal stress resistance in Pseudomonas protegens SN15-2
Danyan Tang, Xiaobing Wang, Jian Wang, Mengfei Wang, Yaping Wang, Wei Wang
Journal of Biosciences.2020;[Epub] CrossRef
Metabolic acclimation of anammox consortia to decreased temperature
Tangran Huo, Yunpeng Zhao, Xi Tang, Huazhang Zhao, Shouqing Ni, Qiang Gao, Sitong Liu
Environment International.2020; 143: 105915. CrossRef
Effects of Different Preservation Methods on Physicochemical Property of Marine Pathogen Vibrio anguillarum
Yongxiang Yu, Zheng Zhang, Yingeng Wang, Meijie Liao, Xiaojun Rong, Bin Li, Hao Zhang
Journal of Ocean University of China.2019; 18(6): 1417. CrossRef
Common Source of Cryoprotection and Osmoprotection by Osmolytes
Roy Govrin, Tal Obstbaum, Uri Sivan
Journal of the American Chemical Society.2019; 141(34): 13311. CrossRef
Selection of DNAzymes for Sensing Aquatic Bacteria: Vibrio Anguillarum
Lide Gu, Wanli Yan, Hangjie Wu, Shihui Fan, Wei Ren, Shujun Wang, Mingsheng Lyu, Juewen Liu
Analytical Chemistry.2019; 91(12): 7887. CrossRef
Vibrio zhugei sp. nov., a moderately halophilic bacterium isolated from pickling sauce
Zhuang Guo, Weicheng Li, Yurong Wang, Qiangchuan Hou, Huijun Zhao, Zhihong Sun, Zhendong Zhang
International Journal of Systematic and Evolutionary Microbiology.2019; 69(5): 1313. CrossRef
Changes in Transcriptome of Yersinia pseudotuberculosis IP32953 Grown at 3 and 28°C Detected by RNA Sequencing Shed Light on Cold Adaptation
Jussa-Pekka Virtanen, Riikka Keto-Timonen, Kaisa Jaakkola, Noora Salin, Hannu Korkeala
Frontiers in Cellular and Infection Microbiology.2018;[Epub] CrossRef
Transcriptome changes in response to temperature in the fish pathogen Photobacterium damselae subsp. damselae: Clues to understand the emergence of disease outbreaks at increased seawater temperatures
Xosé M. Matanza, Carlos R. Osorio, Diogo Neves Proença
PLOS ONE.2018; 13(12): e0210118. CrossRef
Stationary phase-dependent accumulation of ectoine is an efficient adaptation strategy in Vibrio anguillarum against cold stress
Yue Ma, Qiyao Wang, Wensheng Xu, Xiaohong Liu, Xiating Gao, Yuanxing Zhang
Microbiological Research.2017; 205: 8. CrossRef

Research Support, Non-U.S. Gov't

Identification and Characterization of Ectoine Biosynthesis Genes and Heterologous Expression of the ectABC Gene Cluster from Halomonas sp. QHL1, a Moderately Halophilic Bacterium Isolated from Qinghai Lake: Derui Zhu , Jian Liu , Rui Han , Guoping Shen , Qifu Long , Xiaoxing Wei , Deli Liu; J. Microbiol. 2014;52(2):139-147. Published online February 1, 2014; DOI: https://doi.org/10.1007/s12275-014-3389-5

65 View
0 Download
16 Crossref

Abstract

The moderately halophilic bacterium Halomonas sp. QHL1 was identified as a member of the genus Halomonas by 16S rRNA gene sequencing. HPLC analysis showed that strain QHL1 synthesizes ectoine in its cytoplasm. The genes involved in the ectoine biosynthesis pathway were identified on the chromosome in the order ectABC. Subsequently, the ectB gene from this strain was amplified by PCR, and the entire ectABC gene cluster (3,580 bp) was cloned using genome walking. Analysis showed that the ectA (579 bp), ectB (1269 bp), and ectC (390 bp) genes were organized in a single transcriptional unit and were predicted to encode three peptides of 21.2 kDa, 46.4 kDa, and 14.7 kDa, respectively. Two putative promoters, a δ70-dependent promoter and a δ38-controlled promoter, as well as several conserved motifs with unknown function were identified. Individual ectA, ectB, and ectC genes, and the entire ectABC gene cluster were inserted into the expression plasmid pET-28a(+) to generate the recombinant plasmids pET-28a(+)-ectA, pET-28a(+)-ectB, pET-28a(+)-ectC and pET-28a(+)-ectABC, respectively. Heterologous expression of these proteins in Escherichia coli BL21 (DE3) was confirmed by SDS-PAGE. The recombinant E. coli strain BL21 (pET-28a (+)-ectABC) displayed a higher salt tolerance than native E. coli cells but produced far less ectoine than the wild-type QHL1 strain.

Citations

Citations to this article as recorded by

Biotechnological production of ectoine: current status and prospects
Jun Chen, Deliang Qiao, Tao Yuan, Yeyuan Feng, Pengjun Zhang, Xuejun Wang, Li Zhang
Folia Microbiologica.2024; 69(2): 247. CrossRef
Comparative genomic analysis of Halomonas campaniensis wild-type and ultraviolet radiation-mutated strains reveal genomic differences associated with increased ectoine production
Zhibo Wang, Yongzhen Li, Xiang Gao, Jiangwa Xing, Rong Wang, Derui Zhu, Guoping Shen
International Microbiology.2023; 26(4): 1009. CrossRef
Comparative Genome Analysis of a Novel Alkaliphilic Actinobacterial Species Nesterenkonia haasae
Shuang Wang, Lei Sun, Manik Prabhu Narsing Rao, Bao‑zhu Fang, Wen‑jun Li
Polish Journal of Microbiology.2022; 71(3): 453. CrossRef
Whole genome sequencing of the halophilic Halomonas qaidamensis XH36, a novel species strain with high ectoine production
Tiantian Zhang, Tianqi Cui, Yaning Cao, Yongzhen Li, Fenghui Li, Derui Zhu, Jiangwa Xing
Antonie van Leeuwenhoek.2022; 115(4): 545. CrossRef
Identification and characterization of an ectoine biosynthesis gene cluster from Aestuariispira ectoiniformans sp. nov., isolated from seawater
Ji Young Kang, Binna Lee, Jeong Ah Kim, Min-Soo Kim, Chul Ho Kim
Microbiological Research.2022; 254: 126898. CrossRef
Genome Sequence of Streptomyces sp. Strain GQFP Isolated from Soil Near the Roots of Pharmaceutical Plant Elaeagnus pungens
Jie Zhu, David A. Baltrus
Microbiology Resource Announcements.2022;[Epub] CrossRef
Enhanced production of ectoine from methane using metabolically engineered Methylomicrobium alcaliphilum 20Z
Sukhyeong Cho, Yun Seo Lee, Hanyu Chai, Sang Eun Lim, Jeong Geol Na, Jinwon Lee
Biotechnology for Biofuels and Bioproducts.2022;[Epub] CrossRef
Ectoine Production Using Novel Heterologous EctABCS. salarius from Marine Bacterium Salinicola salarius
Yue Su, Wenting Peng, Tong Wang, Yanhui Li, Luyu Zhao, Xinyu Wang, Ying Li, Ling Lin
Applied Sciences.2021; 11(15): 6873. CrossRef
High ectoine production by an engineered Halomonas hydrothermalis Y2 in a reduced salinity medium
Qi Zhao, Shannan Li, Peiwen Lv, Simian Sun, Cuiqing Ma, Ping Xu, Haijun Su, Chunyu Yang
Microbial Cell Factories.2019;[Epub] CrossRef
Analysis of Zobellella denitrificans ZD1 draft genome: Genes and gene clusters responsible for high polyhydroxybutyrate (PHB) production from glycerol under saline conditions and its CRISPR-Cas system
Yu-Wei Wu, Shih-Hung Yang, Myung Hwangbo, Kung-Hui Chu, Chih-Horng Kuo
PLOS ONE.2019; 14(9): e0222143. CrossRef
Engineering the Salt-Inducible Ectoine Promoter Region of Halomonas elongata for Protein Expression in a Unique Stabilizing Environment
Lisa Stiller, Erwin Galinski, Elisabeth Witt
Genes.2018; 9(4): 184. CrossRef
New Alpiniamides From Streptomyces sp. IB2014/011-12 Assembled by an Unusual Hybrid Non-ribosomal Peptide Synthetase Trans-AT Polyketide Synthase Enzyme
Constanze Paulus, Yuriy Rebets, Josef Zapp, Christian Rückert, Jörn Kalinowski, Andriy Luzhetskyy
Frontiers in Microbiology.2018;[Epub] CrossRef
Metabolic engineering of Escherichia coli for L-tryptophan production
V. B. Panichkin, V. A. Livshits, I. V. Biryukova, S. V. Mashko
Applied Biochemistry and Microbiology.2016; 52(9): 783. CrossRef
Draft Genome Sequence of Halomonas elongata Strain K4, an Endophytic Growth-Promoting Bacterium Enhancing Salinity Tolerance In Planta
Feras F. Lafi, Juan S. Ramirez-Prado, Intikhab Alam, Vladimir B. Bajic, Heribert Hirt, Maged M. Saad
Genome Announcements.2016;[Epub] CrossRef
High production of ectoine from aspartate and glycerol by use of whole-cell biocatalysis in recombinant Escherichia coli
Yong-Zhi He, Jiao Gong, Hai-Ying Yu, Yong Tao, Shan Zhang, Zhi-Yang Dong
Microbial Cell Factories.2015;[Epub] CrossRef
Design of an ectoine-responsive AraC mutant and its application in metabolic engineering of ectoine biosynthesis
Wei Chen, Shan Zhang, Peixia Jiang, Jun Yao, Yongzhi He, Lincai Chen, Xiwu Gui, Zhiyang Dong, Shuang-Yan Tang
Metabolic Engineering.2015; 30: 149. CrossRef

Journal Article

Growth and Physiological Properties of Wild Type and Mutants of Halomonas subglaciescola DH-1 in Saline Environment: Hye Jeong Ryu , Yoo Jung Jeong , Doo Hyun Park; J. Microbiol. 2004;42(3):174-180.; DOI: https://doi.org/2093 [pii]

50 View
0 Download

Abstract: A halophilic bacterium was isolated from fermented seafood. The 16S rDNA sequence identity between the isolate and Halomonas subglaciescola AJ306801 was above 95%. The isolate that did not grow in the condition without NaCl or in the condition with other sodium (Na^+) or chloride ions (Cl^-) instead of NaCl was named H. subglaciescola DH-1. Two mutants capable of growing without NaCl were obtained by random mutagenesis, of which their total soluble protein profiles were compared with those of the wild type by two-dimensional electrophoresis. The external compatible solutes (betaine and choline) and cell extract of the wild type did not function as osmoprotectants, and these parameters within the mutants did not enhance their growth in the saline environment. In the proton translocation test, rapid acidification of the reactant was not detected for the wild type, but it was detected for the mutant in the condition without NaCl. From these results, we derived the hypothesis that NaCl may be absolutely required for the energy metabolism of H. subglaciescola DH-1 but not for its osmoregulation, and the mutants may have another modified proton translocation system that is independent of NaCl, except for those mutants with an NaCl-dependent system.

First
Prev
Page of 1
Next
Last

Search