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- DNA vaccine dual-expressing viral hemorrhagic septicemia virus glycoprotein and C-C motif chemokine ligand 19 induces the expression of immune-related genes in zebrafish (Danio rerio)
- Jin-Young Kim , Hyoung Jun Kim , Jeong Su Park , Se Ryun Kwon
- J. Microbiol. 2022;60(10):1032-1038. Published online August 1, 2022
- DOI: https://doi.org/10.1007/s12275-022-2231-8
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- 5 Web of Science
- 3 Crossref
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Abstract
- Glycoprotein (G protein)-based DNA vaccines are effective in protecting aquaculture fish from rhabdoviruses but the degree of immune response they elicit depends on plasmid concentration and antigen cassette. Here, we developed a DNA vaccine using the viral hemorrhagic septicemia virus G (VG) gene and chemokine (C-C motif) ligand 19 (CCL19)a.2 regulated by the CMV promoter as the molecular adjuvant. After transfection of the prepared plasmid (pVG + CCL19) into epithelioma papulosum cyprini cells, mRNA expression was confirmed through quantitative real-time polymerase chain reaction. The vaccine was intramuscularly injected into zebrafish (Danio rerio), and 28 days after immunization, viral hemorrhagic septicemia virus (105 TCID50/10 μl/fish) was intraperitoneally injected. A survival rate of 68% was observed in the pVG + CCL19 group but this was not significantly different from the survival rate of fish treated with pVG alone, that is, without the adjuvant. However, the expression of interferonand cytokine-related genes in the spleen and kidney tissues of zebrafish was significantly increased (p < 0.05) on days 1, 3, 7, and 14 after immunization. Thus, CCL19a.2 induced an initial immune response as a molecular adjuvant, which may provide initial protection against virus infection before vaccination- induced antibody formation. This study provides insights on the functions of CCL19a.2 adjuvant in DNA vaccines.
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Citations
Citations to this article as recorded by
- LncRNA activates immune response against Vibrio anguillarum in the intestine-liver axis of turbot (Scophthalmus maximus L.) by sponging miRNA in a ceRNA regulatory network
Xin Cai, Chengbin Gao, Alan J. Lymbery, Le Ma, Qiang Fu, Ranran Huang, Chao Li
Aquaculture.2023; 576: 739882. CrossRef - Determining transcriptomic response of kidneys of olive flounder to viral hemorrhagic septicemia virus infection using next-generation sequencing
Hyoung Jun Kim, Jeong Su Park, Se Ryun Kwon, Youngjin Park
Aquaculture.2023; 562: 738886. CrossRef - Integrative transcriptomic profiling reveals the key pathways in the regulation mechanism of fish intestine-spleen immunity during the bacterial challenges
Chengbin Gao, Xin Cai, Alan J. Lymbery, Le Ma, Min Cao, Chao Li
Aquaculture.2023; 568: 739320. CrossRef
- LncRNA activates immune response against Vibrio anguillarum in the intestine-liver axis of turbot (Scophthalmus maximus L.) by sponging miRNA in a ceRNA regulatory network
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