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- Vibrio parahaemolyticus cqsA controls production of quorum sensing signal molecule 3-hydroxyundecan-4-one and regulatessensing signal molecule 3-hydroxyundecan-4-one and regulates colony morphology
- Kui Wu , Yangyun Zheng , Qingping Wu , Haiying Chen , Songzhe Fu , Biao Kan , Yongyan Long , Xiansheng Ni , Junling Tu
- J. Microbiol. 2019;57(12):1105-1114. Published online November 4, 2019
- DOI: https://doi.org/10.1007/s12275-019-9379-x
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- 12 Web of Science
- 11 Crossref
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Abstract
-
In order to adapt to different environments, Vibrio parahaemolyticus
employed a complicated quorum sensing system to
orchestrate gene expression and diverse colony morphology
patterns. In this study, the function of the putative quorum
sensing signal synthase gene cqsA (VPA0711 in V. parahaemolyticus
strain RIMD2210633 genome) was investigated.
The cloning and expression of V. parahaemolyticus cqsA in
Escherichia coli system induced the production of a new quorum
sensing signal that was found in its culture supernatant.
The signal was purified by high performance liquid chromatography
methods
and determined to be 3-hydroxyundecan- 4-one by indirect and direct mass spectra assays. The deletion of cqsA in RIMD2210633 changed V. parahaemolyticus colony morphology from the classical ‘fried-egg’ shape (thick and opaque in the center, while thin and translucent in the edge) of the wild-type colony to a ‘pancake’ shape (no significant difference between the centre and the edge) of the cqsAdeleted colony. This morphological change could be restored by complementary experiment with cqsA gene or the signal extract. In addition, the expression of opaR, a well-known quorum sensing regulatory gene, could be up-regulated by cqsA deletion. Our results suggested that V. parahaemolyticus used cqsA to produce 3-hydroxyundecan-4-one signal and thereby regulated colony morphology and other quorum sensing-associated behaviors. -
Citations
Citations to this article as recorded by
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PeerJ.2021; 9: e11567. CrossRef -
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Populations in Shellfish Farms
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mSystems.2021;[Epub] CrossRef
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