Skip Navigation
Skip to contents

Journal of Microbiology : Journal of Microbiology

OPEN ACCESS
SEARCH
Search

Search

Page Path
HOME > Search
1 "cyclic AMP receptor protein"
Filter
Filter
Article category
Keywords
Publication year
Journal Article
Regulatory Role of cAMP Receptor Protein over Escherichia coli Fumarase Genes
Yu-Pei Chen , Hsiao-Hsien Lin , Chi-Dung Yang , Shin-Hong Huang , Ching-Ping Tseng
J. Microbiol. 2012;50(3):426-433.   Published online June 30, 2012
DOI: https://doi.org/10.1007/s12275-012-1542-6
  • 13 View
  • 0 Download
  • 8 Citations
AbstractAbstract
Escherichia coli expresses three fumarase genes, namely, fumA, fumB, and fumC. In the present study, catabolite repression was observed in the fumA-lacZ and fumC-lacZ fusion strains, but not in the fumB-lacZ fusion strain. The Crp-binding sites in fumA and fumC were identified using an electrophoretic mobility shift assay and footprint analysis. However, the electrophoretic mobility shift assay did not detect band shifts in fumB. Fnr and ArcA serve as transcription regulators of fumarase gene expression. In relation to this, different mutants, including Δcya, Δcrp, Δfnr, and ΔarcA, were used to explore the regulatory role of Crp over fumA and fumC. The results show that Crp is an activator of fumA and fumC gene expression under various oxygen conditions and growth rates. ArcA was identified as the dominant repressor, with the major repression occurring at 0–4% oxygen. In addition, Fnr was confirmed as a repressor of fumC for the first time. This study elucidates the effects of Crp on fumarase gene expression.

Journal of Microbiology : Journal of Microbiology
TOP