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- Agromyces laixinhei sp. nov. isolated from bat feces in China
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Yanpeng Cheng , Yibo Bai , Yuyuan Huang , Jing Yang , Shan Lu , Dong Jin , Ji Pu , Han Zheng , Junqin Li , Ying Huang , Suping Wang , Jianguo Xu
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J. Microbiol. 2021;59(5):467-475. Published online March 29, 2021
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DOI: https://doi.org/10.1007/s12275-021-0546-5
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Abstract
- Three rod-shaped, Gram-stain-positive, and catalase-positive,
phenotypically closely related isolates (HY052T, HY050, and
HY045) were obtained from fecal samples collected from bats
in Guangxi province and Chongqing city of China. Circular,
smooth, light-yellow colonies appeared on brain heart infusion
plate after 24–48 h incubation at 28°C. The optimal pH
for growth was between 6.0 and 7.5. Based on 16S rRNA, the
three isolates were phylogenetically related to Agromyces terreus
DS-10T, Agromyces aureus AR33T, Agromyces salentinus
20-5T, Agromyces allii UMS-62T, Agromyces lapidis CD55T,
and Agromyces italicus CD1T. Moreover, based on 296 core
genes, the phylogenomic tree indicated that the three isolates
clustered together, closest to Agromyces cerinus VKM Ac-
1340T and Agromyces fucosus VKM Ac-1345T but separated
distantly from other Agromyces species. The average nucleotide
identity values between strain HY052T and other Agromyces
species ranged from 79.3% to 87.9%, lower than the
95–96% threshold. Furthermore, the genome of strain HY052T
contains a circular chromosome of 3,437,203 bp with G + C
content of 69.0 mol%. Main fatty acids were anteiso-C15:0
and anteiso-C17:0. The polar lipids comprised diphosphatidylglycerol,
phosphatidylglycerol, and unidentified glycolipids.
Rhamnose, ribose, and glucose were the primary cell wall sugars.
The major peptidoglycan amino acids included alanine,
glutamic acid, glycine, and 2,4-diaminobutyric acid. An additional
remarkable difference from other Agromyces species
is that MK-12 was the sole menaquinone in strain HY052T.
Based on results from the polyphasic characterizations performed
in this study, our isolates are proposed to be members
of a novel species in genus Agromyces, named Agromyces
laixinhei. The type strain is HY052T (= CGMCC 1.17175T =
JCM 33695T).
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