Journal of Microbiology

Journal Article

Diagnosis and molecular characteristics of human infections caused by Anaplasma phagocytophilum in South Korea: Seung Hun Lee , Sungdo Park , Yeong Seon Lee , Hae Kyung Lee , Seon Do Hwang; J. Microbiol. 2018;56(11):847-853. Published online October 24, 2018; DOI: https://doi.org/10.1007/s12275-018-8385-8

44 View
0 Download
11 Crossref

Human granulocytic anaplasmosis (HGA) is a tick borne infection caused by Anaplasma phagocytophilum. HGA cases in South Korea have been identified since the first report in 2014. In this study, we investigated the serological response in 594 clinical samples of patients with acute febrile illness and molecular characteristics of A. phagocytophilum clinical isolates obtained from HGA patients. In serological test for A. phagocytophilum, 7.91% (47/594 cases) were positive for IgG and Ig M and 13 of 47 cases showed seroconversion. In the detection rate of the 16S rRNA, msp2(p44), and ankA, genes were showed 3.68% (14/380 cases) for A. phagocytophilum- specific 16S rRNA gene. Phylogenetic analysis of three clinical isolates demonstrated high sequence similarity (98.58– 100%) with A. phagocytophilum 16S rRNA sequences identified from public databases. Analysis of the msp2(p44) gene showed highly variable similarity rates (7.24–98.85%) even within isolated countries and host ranges. These results provide clues into the bacterial characterization of A. phagocytophilum originating from Korean patients, providing useful guidance for treatment and improving clinical outcomes.

Citations

Citations to this article as recorded by

Human granulocytotropic anaplasmosis—A systematic review and analysis of the literature
Sophie Schudel, Larissa Gygax, Christian Kositz, Esther Kuenzli, Andreas Neumayr, Ana LTO Nascimento
PLOS Neglected Tropical Diseases.2024; 18(8): e0012313. CrossRef
Molecular and serological detection of Anaplasma spp. in small ruminants in an area of Cerrado Biome in northeastern Brazil
Ellainy Maria Conceição Silva, Ingrid Carolinne Lopes Marques, Victória Valente Califre de Mello, Renan Bressianini do Amaral, Luiz Ricardo Gonçalves, Maria do Socorro Costa Oliveira Braga, Larissa Sarmento dos Santos Ribeiro, Rosangela Zacarias Machado,
Ticks and Tick-borne Diseases.2024; 15(1): 102254. CrossRef
Global status of Anaplasma phagocytophilum infections in human population: A 50-year (1970–2020) meta-analysis
Solomon Ngutor Karshima, Musa Isiyaku Ahmed, Kaltume Mamman Mohammed, Victoria Adamu Pam
Journal of Vector Borne Diseases.2023; 60(3): 265. CrossRef
Human granulocytic anaplasmosis in a Single University Hospital in the Republic of Korea
Da Young Kim, Jun-Won Seo, Na Ra Yun, Choon-Mee Kim, Dong-Min Kim
Scientific Reports.2021;[Epub] CrossRef
Development of a duplex PCR assay for detecting Theileria luwenshuni and Anaplasma phagocytophilum in sheep and goats
Yaqun Yan, Yanyan Cui, Shanshan Zhao, Jichun Jing, Ke Shi, Fuchun Jian, Longxian Zhang, Rongjun Wang, Kunlun Wang, Yongchun Zhou, Changshen Ning
Experimental and Applied Acarology.2021; 85(2-4): 319. CrossRef
The Novel Zoonotic Pathogen, Anaplasma capra, Infects Human Erythrocytes, HL-60, and TF-1 Cells In Vitro
Yongshuai Peng, Chenyang Lu, Yaqun Yan, Jinxing Song, Zhiyang Pei, Pihong Gong, Rongjun Wang, Longxian Zhang, Fuchun Jian, Changshen Ning
Pathogens.2021; 10(5): 600. CrossRef
Parasitic and Vector-Borne Infections in HIV-Positive Patients in Slovakia—Evidence of an Unexpectedly High Occurrence of Anaplasma phagocytophilum
Katarína Šimeková, Ľubomír Soják, Bronislava Víchová, Lenka Balogová, Júlia Jarošová, Daniela Antolová
Pathogens.2021; 10(12): 1557. CrossRef
Are other tick-borne infections overlooked in patients investigated for Lyme neuroborreliosis? A large retrospective study from South-eastern Sweden
Paula Gyllemark, Peter Wilhelmsson, Camilla Elm, Dieuwertje Hoornstra, Joppe W. Hovius, Marcus Johansson, Ivar Tjernberg, Per-Eric Lindgren, Anna J. Henningsson, Johanna Sjöwall
Ticks and Tick-borne Diseases.2021; 12(5): 101759. CrossRef
A Multiplex PCR Detection Assay for the Identification of Clinically Relevant Anaplasma Species in Field Blood Samples
Yongshuai Peng, Shanshan Zhao, Kunlun Wang, Jinxing Song, Yaqun Yan, Yongchun Zhou, Ke Shi, Fuchun Jian, Rongjun Wang, Longxian Zhang, Changshen Ning
Frontiers in Microbiology.2020;[Epub] CrossRef
Human granulocytic anaplasmosis in Kinmen, an offshore island of Taiwan
Kun-Hsien Tsai, Lo-Hsuan Chung, Chia-Hao Chien, Yu-Jung Tung, Hsin-Yi Wei, Tsai-Ying Yen, Pei-Yun Shu, Hsi-Chieh Wang, José Reck
PLOS Neglected Tropical Diseases.2019; 13(9): e0007728. CrossRef
Co-Infection of Scrub Typhus and Human Granulocytic Anaplasmosis in Korea, 2006
Jeong-Han Kim, Chang-Seop Lee, Chisook Moon, Yee Gyung Kwak, Baek-Nam Kim, Eu Suk Kim, Jae Myung Kang, Wan Beom Park, Myoung-don Oh, Sang-Won Park
Journal of Korean Medical Science.2019;[Epub] CrossRef

Reviews

MINIREVIEW] Importance of differential identification of Mycobacterium tuberculosis strains for understanding differences in their prevalence, treatment efficacy, and vaccine development: Hansong Chae , Sung Jae Shin; J. Microbiol. 2018;56(5):300-311. Published online May 2, 2018; DOI: https://doi.org/10.1007/s12275-018-8041-3

51 View
0 Download
19 Crossref

Abstract

Tuberculosis (TB), caused by Mycobacterium tuberculosis (Mtb), remains a serious global health problem in the 21st century because of its high mortality. Mtb is an extremely successful human-adapted pathogen that displays a multifactorial ability to control the host immune response and to evade killing by drugs, resulting in the breakdown of BCG vaccine-conferred anti-TB immunity and development of multidrug-resistant (MDR) and extensively drug-resistant (XDR) Mtb. Although genetic components of the genomes of the Mtb complex strains are highly conserved, showing over 99% similarity to other bacterial genera, recently accumulated evidence suggests that the genetic diversity of the Mtb complex strains has implications for treatment outcomes, development of MDR/XDR Mtb, BCG vaccine efficacy, transmissibility, and epidemiological outbreaks. Thus, new insights into the pathophysiological features of the Mtb complex strains are required for development of novel vaccines and for control of MDR/XDR Mtb infection, eventually leading to refinement of treatment regimens and the health care system. Many studies have focused on the differential identification of Mtb complex strains belonging to different lineages because of differences in their virulence and geographical dominance. In this review, we discuss the impact of differing genetic characteristics among Mtb complex strains on vaccine efficacy, treatment outcome, development of MDR/ XDR Mtb strains, and epidemiological outbreaks by focusing on the best-adapted human Mtb lineages. We further explore the rationale for differential identification of Mtb strains for more effective control of TB in clinical and laboratory settings by scrutinizing current diagnostic methods.

Citations

Citations to this article as recorded by

Assistance of next-generation sequencing for diagnosis of disseminated Bacillus Calmette-Guerin disease with X-SCID in an infant: a case report and literature review
Haiyang Zhang, Yi Liao, Zhensheng Zhu, Hanmin Liu, Deyuan Li, Sisi Wang
Frontiers in Cellular and Infection Microbiology.2024;[Epub] CrossRef
Applications and advances in molecular diagnostics: revolutionizing non-tuberculous mycobacteria species and subspecies identification
Haiyang Zhang, Maoting Tang, Deyuan Li, Min Xu, Yusen Ao, Liangkang Lin
Frontiers in Public Health.2024;[Epub] CrossRef
Distinct contributions of the innate immune receptors TLR2 and RP105 to formation and architecture of structured lung granulomas in mice infected with Mycobacterium tuberculosis
Meg L. Donovan, Helle Bielefeldt‐Ohmann, Rachel F. Rollo, Stephen J. McPherson, Thomas E. Schultz, Giorgia Mori, Jessica C. Kling, Antje Blumenthal
Immunology.2023; 169(1): 13. CrossRef
Host vs. pathogen evolutionary arms race: Effects of exposure history on individual response to a genetically diverse pathogen
Daniel P. Walsh, Brandi L. Felts, E. Frances Cassirer, Thomas E. Besser, Jonathan A. Jenks
Frontiers in Ecology and Evolution.2023;[Epub] CrossRef
Antimycobacterial Activity of Hedeoma drummondii against Mycobacterium tuberculosis and Non-Tuberculous Mycobacteria
Carmen Molina-Torres, Carlos Pedraza-Rodríguez, Lucio Vera-Cabrera, Jorge Ocampo-Candiani, Catalina Rivas-Morales, Ezequiel Viveros-Valdez
Antibiotics.2023; 12(5): 833. CrossRef
Mycobacterium tuberculosis lineage 4 associated with cavitations and treatment failure
Anabel Ordaz-Vázquez, Pedro Torres-González, Leticia Ferreyra-Reyes, Sergio Canizales-Quintero, Guadalupe Delgado-Sánchez, Lourdes García-García, Alfredo Ponce-De-León, José Sifuentes-Osornio, Miriam Bobadilla-Del-Valle
BMC Infectious Diseases.2023;[Epub] CrossRef
Will we ever eradicate animal tuberculosis?
Christian Gortázar, José de la Fuente, Alberto Perelló, Lucas Domínguez
Irish Veterinary Journal.2023;[Epub] CrossRef
Predominance of the Mycobacterium tuberculosis Beijing strain amongst children from a high tuberculosis burden township in South Africa
Junaid Shaik, Manormoney Pillay, Julie Moodley, Prakash Jeena
Tuberculosis.2022; 136: 102250. CrossRef
Dysglycemia is associated with Mycobacterium tuberculosis lineages in tuberculosis patients of North Lima—Peru
Kattya Lopez, María B. Arriaga, Juan G. Aliaga, Nadia N. Barreda, Oswaldo M. Sanabria, Chuan-Chin Huang, Zibiao Zhang, Ruth García-de-la-Guarda, Leonid Lecca, Anna Cristina Calçada Carvalho, Afrânio L. Kritski, Roger I. Calderon, Igor Mokrousov
PLOS ONE.2021; 16(1): e0243184. CrossRef
In vitro Synergism of Six Antituberculosis Agents Against Drug-Resistant Mycobacterium tuberculosis Isolated from Retreatment Tuberculosis Patients
Ruoyan Ying, Xiaochen Huang, Yaxian Gao, Jie Wang, Yidian Liu, Wei Sha, Hua Yang
Infection and Drug Resistance.2021; Volume 14: 3729. CrossRef
Characterisation of secretome-based immune responses of human leukocytes infected with variousMycobacterium tuberculosislineages
Benjawan Kaewseekhao, Sittiruk Roytrakul, Yodying Yingchutrakul, Marut Laohaviroj, Kanin Salao, Kiatichai Faksri
PeerJ.2021; 9: e11565. CrossRef
Different PPD-stimulated cytokine responses from patients infected with genetically distinct Mycobacterium tuberculosis complex lineages
Paulo Ranaivomanana, Marie Sylvianne Rabodoarivelo, Mame Diarra Bousso Ndiaye, Niaina Rakotosamimanana, Voahangy Rasolofo
International Journal of Infectious Diseases.2021; 104: 725. CrossRef
A review of published spoligotype data indicates the diversity of Mycobacterium tuberculosis from India is under-represented in global databases
Husain Poonawala, Narender Kumar, Sharon J. Peacock
Infection, Genetics and Evolution.2020; 78: 104072. CrossRef
Comparing IS6110‐RFLP, PGRS‐RFLP and IS6110‐Mtb1/Mtb2 PCR methods for genotyping ofMycobacterium tuberculosisisolates
Kh. Ansarin, L. Sahebi, Y. Aftabi, M. Khalili, M. Seyyedi
Journal of Applied Microbiology.2020; 129(4): 1062. CrossRef
Molecular Typing of Mycobacterium Tuberculosis Isolated from Iranian Patients Using Highly Abundant Polymorphic GC-Rich-Repetitive Sequence
Bahram Golestani Eimani, Khalil Ansarin, Leila Sahebi, Maryam Seyyedi
Iranian South Medical Journal.2020; 23(2): 87. CrossRef
Comparison of the Three Molecular Diagnostic Assays for Molecular Identification ofMycobacterium tuberculosisand Nontuberculous Mycobacteria Species in Sputum Samples
Jinyoung Bae, Sung-Bae Park, Ji-Hoi Kim, Mi Ran Kang, Kyung Eun Lee, Sunghyun Kim, Hyunwoo Jin
Biomedical Science Letters.2020; 26(3): 170. CrossRef
Immunogenicity and Vaccine Potential of InsB, an ESAT-6-Like Antigen Identified in the Highly Virulent Mycobacterium tuberculosis Beijing K Strain
Woo Sik Kim, Hongmin Kim, Kee Woong Kwon, Sang-Nae Cho, Sung Jae Shin
Frontiers in Microbiology.2019;[Epub] CrossRef
Molecular characterisation of multidrug-resistantMycobacterium tuberculosisisolates from a high-burden tuberculosis state in Brazil
R. S. Salvato, S. Schiefelbein, R. B. Barcellos, B. M. Praetzel, I. S. Anusca, L. S. Esteves, M. L. Halon, G. Unis, C.F. Dias, S. S. Miranda, I. N. de Almeida, L. J. de Assis Figueredo, E. C. Silva, A. L. Kritski, E. R. Dalla Costa, M. L. R. Rossetti
Epidemiology and Infection.2019;[Epub] CrossRef
DNA markers for tuberculosis diagnosis
Kai Ling Chin, Maria E. Sarmiento, Mohd Nor Norazmi, Armando Acosta
Tuberculosis.2018; 113: 139. CrossRef

REVIEW] The role of laboratory diagnostics in emerging viral infections: the example of the Middle East respiratory syndrome epidemic: Jasper F. W. Chan , Siddharth Sridhar , Cyril C. Y. Yip , Susanna K. P. Lau , Patrick C. Y. Woo; J. Microbiol. 2017;55(3):172-182. Published online February 28, 2017; DOI: https://doi.org/10.1007/s12275-017-7026-y

45 View
0 Download
22 Crossref

Abstract

Rapidly emerging infectious disease outbreaks place a great strain on laboratories to develop and implement sensitive and specific diagnostic tests for patient management and infection control in a timely manner. Furthermore, laboratories also play a role in real-time zoonotic, environmental, and epidemiological investigations to identify the ultimate source of the epidemic, facilitating measures to eventually control the outbreak. Each assay modality has unique pros and cons; therefore, incorporation of a battery of tests using traditional culture-based, molecular and serological diagnostics into diagnostic algorithms is often required. As such, laboratories face challenges in assay development, test evaluation, and subsequent quality assurance. In this review, we describe the different testing modalities available for the ongoing Middle East respiratory syndrome (MERS) epidemic including cell culture, nucleic acid amplification, antigen detection, and antibody detection assays. Applications of such tests in both acute clinical and epidemiological investigation settings are highlighted. Using the MERS epidemic as an example, we illustrate the various challenges faced by laboratories in test development and implementation in the setting of a rapidly emerging infectious disease. Future directions in the diagnosis of MERS and other emerging infectious disease investigations are also highlighted.

Citations

Citations to this article as recorded by

Scale-Up of COVID-19 Testing Services in NYC, 2020–2021: Lessons Learned to Maximize Reach, Equity and Timeliness
Lorna E. Thorpe, Sarah Conderino, Stefanie Bendik, Carolyn Berry, Nadia Islam, Rachel Massar, Michelle Chau, Rita Larson, Margaret M. Paul, Chuan Hong, Andrew Fair, Andrea R. Titus, Anna Bershteyn, Andrew Wallach
Journal of Urban Health.2024; 101(5): 913. CrossRef
An individual level infectious disease model in the presence of uncertainty from multiple, imperfect diagnostic tests
Caitlin Ward, Grant D. Brown, Jacob J. Oleson
Biometrics.2023; 79(1): 426. CrossRef
A comparative study of the policy response to COVID-19 in the ASEAN region: A dynamic simulated ARDL approach
Nihal Ahmed, Dilawar Khan, Judit Oláh, József Popp, Stefan Cristian Gherghina
PLOS ONE.2023; 18(1): e0276973. CrossRef
RNA Coronaviruses’ Outbreaks: Recent Progress on the SARS-CoV-2 Pandemic Diagnostic Tests, Vaccination and Therapeutics
Ghadeer A.R.Y. Suaifan, Bayan A. Alkhawaja, Aya A.M. Mohammed
Mini-Reviews in Medicinal Chemistry.2022; 22(4): 617. CrossRef
A computational tool for trend analysis and forecast of the COVID-19 pandemic
Henrique Mohallem Paiva, Rubens Junqueira Magalhães Afonso, Fabiana Mara Scarpelli de Lima Alvarenga Caldeira, Ester de Andrade Velasquez
Applied Soft Computing.2021; 105: 107289. CrossRef
Commercially available rapid diagnostic tests for the detection of high priority pathogens: status and challenges
Jaime Castillo-León, Ramona Trebbien, John J. Castillo, Winnie E. Svendsen
The Analyst.2021; 146(12): 3750. CrossRef
A network-based model to explore the role of testing in the epidemiological control of the COVID-19 pandemic
Yapeng Cui, Shunjiang Ni, Shifei Shen
BMC Infectious Diseases.2021;[Epub] CrossRef
Laboratory testing of SARS‐CoV, MERS‐CoV, and SARS‐CoV‐2 (2019‐nCoV): Current status, challenges, and countermeasures
Ying Yan, Le Chang, Lunan Wang
Reviews in Medical Virology.2020;[Epub] CrossRef
Laboratory verification of an RT‐PCR assay for SARS‐CoV‐2
Yingping Wu, Wei Xu, Zhiqiang Zhu, Xiaoping Xia
Journal of Clinical Laboratory Analysis.2020;[Epub] CrossRef
Current diagnostic tools for coronaviruses–From laboratory diagnosis to POC diagnosis for COVID‐19
Yung‐Chih Wang, Yi‐Tzu Lee, Ting Yang, Jun‐Ren Sun, Ching‐Fen Shen, Chao‐Min Cheng
Bioengineering & Translational Medicine.2020;[Epub] CrossRef
The Molecular Diagnosis Protocols of New Coronavirus (COVID-19); Specificity and Sensitivity an Overview
Abdullah Ahmed Hama, Othman Abdulrahman Mohammed, Fatima Mahmud Ali, Osama Hamid Shareef, Sardar Muhammad Wli, Sabiha Sharif, Syamand Ahmed Qadir
Kurdistan Journal of Applied Research.2020; : 13. CrossRef
Thoughts on What Chemists Can Contribute to Fighting SARS‐CoV‐2 – A Short Note on Hand Sanitizers, Drug Candidates and Outreach
Till Opatz, Joerg Senn‐Bilfinger, Clemens Richert
Angewandte Chemie.2020; 132(24): 9320. CrossRef
Thoughts on What Chemists Can Contribute to Fighting SARS‐CoV‐2 – A Short Note on Hand Sanitizers, Drug Candidates and Outreach
Till Opatz, Joerg Senn‐Bilfinger, Clemens Richert
Angewandte Chemie International Edition.2020; 59(24): 9236. CrossRef
An updated roadmap for MERS-CoV research and product development: focus on diagnostics
Cassandra Kelly-Cirino, Laura T Mazzola, Arlene Chua, Christopher J Oxenford, Maria D Van Kerkhove
BMJ Global Health.2019; 4(Suppl 2): e001105. CrossRef
Development and validation of different indirect ELISAs for MERS-CoV serological testing
Anwar M. Hashem, Sawsan S. Al-amri, Tagreed L. Al-subhi, Loai A. Siddiq, Ahmed M. Hassan, Maha M. Alawi, Rowa Y. Alhabbab, Salwa I. Hindawi, Osama B. Mohammed, Nabil S. Amor, Abdulaziz N. Alagaili, Ahmed A. Mirza, Esam I. Azhar
Journal of Immunological Methods.2019; 466: 41. CrossRef
International Biological Reference Preparations for Epidemic Infectious Diseases
Tommy Rampling, Mark Page, Peter Horby
Emerging Infectious Diseases.2019; 25(2): 205. CrossRef
MERS coronavirus outbreak: Implications for emerging viral infections
Awad Al-Omari, Ali A. Rabaan, Samer Salih, Jaffar A. Al-Tawfiq, Ziad A. Memish
Diagnostic Microbiology and Infectious Disease.2019; 93(3): 265. CrossRef
Inclusion of MERS‐spike protein ELISA in algorithm to determine serologic evidence of MERS‐CoV infection
Suvang Trivedi, Congrong Miao, Mohammad M. Al‐Abdallat, Aktham Haddadin, Sultan Alqasrawi, Ibrahim Iblan, Mohannad A. Nsour, Tarek Alsanouri, Sami S. Ali, Brian Rha, Susan I. Gerber, Daniel C. Payne, Azaibi Tamin, Natalie J. Thornburg
Journal of Medical Virology.2018; 90(2): 367. CrossRef
The structure of the C-terminal domain of the nucleoprotein from the Bundibugyo strain of the Ebola virus in complex with a pan-specific synthetic Fab
Malwina J. Radwańska, Mateusz Jaskółowski, Elena Davydova, Urszula Derewenda, Tsuyoshi Miyake, Daniel A. Engel, Anthony A. Kossiakoff, Zygmunt S. Derewenda
Acta Crystallographica Section D Structural Biology.2018; 74(7): 681. CrossRef
MERS, SARS and other coronaviruses as causes of pneumonia
Yudong Yin, Richard G. Wunderink
Respirology.2018; 23(2): 130. CrossRef
Hydrogel Tethering Enhances Interdomain Stabilization of Single-Chain Antibodies
Yijia Xiong, Nicole R. Ford, Karen A. Hecht, Guritno Roesijadi, Thomas C. Squier
Bioconjugate Chemistry.2017; 28(11): 2804. CrossRef
An Opportunistic Pathogen Afforded Ample Opportunities: Middle East Respiratory Syndrome Coronavirus
Ian Mackay, Katherine Arden
Viruses.2017; 9(12): 369. CrossRef

Research Support, Non-U.S. Gov'ts

Performance of PCR-reverse blot hybridization assay for detection of rifampicin-resistant Mycobacterium leprae: Hye-young Wang , Hyunjung Kim , Yeun Kim , Hyeeun Bang , Jong-Pill Kim , Joo Hwan Hwang , Sang-Nae Cho , Tae Ue Kim , Hyeyoung Lee; J. Microbiol. 2015;53(10):686-693. Published online October 2, 2015; DOI: https://doi.org/10.1007/s12275-015-5057-9

50 View
0 Download
1 Crossref

Abstract

Drug resistance in Mycobacterium leprae is a significant problem in countries where leprosy is endemic. A sensitive, specific, and high-throughput reverse blot hybridization assay (REBA) for the detection of genotypic resistance to rifampicin (RIF) was designed and evaluated. It has been shown that resistance to RIF in M. leprae involves mutations in the rpoB gene encoding the β-subunit of the RNA polymerase. The PCR-REBA simultaneously detects both 6 wild-type regions and 5 different mutations (507AGC, 513GTG, 516TAT, 531ATG, and 531TTC) including the most prevalent mutations at positions 507 and 531. Thirty-one clinical isolates provided by Korea Institute of Hansen’s Disease were analyzed by PCR-REBA with RIF resistance of rpoB gene. As a
result
, missense mutations at codons 507 AGC and 531ATG with 2-nucleotide substitutions were found in one sample, and a missense mutation at codon 516 TAT and ΔWT6 (deletion of 530-534) was found in another sample. These cases were confirmed by DNA sequence analysis. This rapid, simple, and highly sensitive assay provides a practical alternative to sequencing for genotypic evaluation of RIF resistance in M. leprae.

Citations

Citations to this article as recorded by

Prediction of Y haplogroup by polymerase chain reaction-reverse blot hybridization assay
Sehee Oh, Jungho Kim, Sunyoung Park, Seoyong Kim, Kyungmyung Lee, Yang-Han Lee, Si-Keun Lim, Hyeyoung Lee
Genes & Genomics.2019; 41(3): 297. CrossRef

Performance of a real-time PCR assay for the rapid identification of Mycobacterium species: Hye-young Wang , Hyunjung Kim , Sunghyun Kim , Do-kyoon Kim , Sang-Nae Cho , Hyeyoung Lee; J. Microbiol. 2015;53(1):38-46. Published online January 4, 2015; DOI: https://doi.org/10.1007/s12275-015-4495-8

44 View
0 Download
18 Crossref

Abstract

Mycobacteria cause a variety of illnesses that differ in severity and public health implications. The differentiation of Mycobacterium tuberculosis (MTB) from nontuberculous mycobacteria (NTM) is of primary importance for infection control and choice of antimicrobial therapy. The diagnosis of diseases caused by NTM is difficult because NTM species are prevalent in the environment and because they have fastidious properties. In the present study, we evaluated 279 clinical isolates grown in liquid culture provided by The Catholic University of Korea, St. Vincent’s Hospital using real-time PCR based on mycobacterial rpoB gene sequences. The positive rate of real-time PCR assay accurately discriminated 100% (195/195) and 100% (84/84) between MTB and NTM species. Comparison of isolates identified using the MolecuTech REBA Myco-ID? and Real Myco-ID? were completely concordant except for two samples. Two cases that were identified as mixed infection (M. intracellulare-M. massiliense and M. avium-M. massiliense co-infection) by PCRREBA assay were only detected using M. abscessus-specific probes by Real Myco-ID?. Among a total of 84 cases, the most frequently identified NTM species were M. intracellulare (n=38, 45.2%), M. avium (n=18, 23.7%), M. massiliense (n=10, 13.2%), M. fortuitum (n=5, 6%), M. abscessus (n=3, 3.9%), M. gordonae (n=3, 3.9%), M. kansasii (n=2, 2.4%), M. mucogenicum (n=2, 2.4%), and M. chelonae (n= 1, 1.2%). Real Myco-ID? is an efficient tool for the rapid detection of NTM species as well as MTB and sensitive and specific and comparable to conventional methods.

Citations

Citations to this article as recorded by

Durlobactam to boost the clinical utility of standard of care β-lactams against Mycobacterium abscessus lung disease
Dereje A. Negatu, Wassihun Wedajo Aragaw, Tewodros T. Gebresilase, Sindhuja Paruchuri, Firat Kaya, Sung Jae Shin, Peter Sander, Véronique Dartois, Thomas Dick, Jared A. Silverman
Antimicrobial Agents and Chemotherapy.2025;[Epub] CrossRef
Evaluation of Nanopore Sequencing for Diagnosing Pulmonary Tuberculosis Using Negative Smear Clinical Specimens
Guocan Yu, Yanqin Shen, Liwei Yao, Xudong Xu
Infection and Drug Resistance.2024; Volume 17: 673. CrossRef
Preclinical murine models for the testing of antimicrobials against Mycobacterium abscessus pulmonary infections: Current practices and recommendations
Véronique Dartois, Tracey L. Bonfield, Jim P. Boyce, Charles L. Daley, Thomas Dick, Mercedes Gonzalez-Juarrero, Shashank Gupta, Igor Kramnik, Gyanu Lamichhane, Barbara E. Laughon, Nicola I. Lorè, Kenneth C. Malcolm, Kenneth N. Olivier, Katherine L. Tuggle
Tuberculosis.2024; 147: 102503. CrossRef
Epidemiology and laboratory detection of non-tuberculous mycobacteria
Nuo Xu, Lihong Li, Shenghai Wu
Heliyon.2024; 10(15): e35311. CrossRef
Molecular identification of non-tuberculous mycobacterial species isolated from extrapulmonary samples using real-time PCR and rpoB sequence analysis
Mohammad Hashemzadeh, Aram Asarehzadegan Dezfuli, Azar Dokht Khosravi, Maryam Moradi Bandbal, Atousa Ghorbani, Mahtab Hamed, Soolmaz Khandan Dezfuli
AMB Express.2023;[Epub] CrossRef
TO ANALYZE THE TB-PCR POSITIVITY RATE USING REAL-TIME PCR FOR EARLY DETECTION OF TUBERCULOSIS
DEEPAK SAWANT, LOKHANDE CD, SHARMA RK, CHOUGULE RA
Asian Journal of Pharmaceutical and Clinical Research.2023; : 167. CrossRef
Factors Associated with the Performance of Direct PCR Detection of Mycobacteria in Clinical Specimens: Retrospective Real-world Data
Chang-Hun Park
Laboratory Medicine Online.2023; 13(3): 205. CrossRef
Evaluation of a new assay for nontuberculous mycobacteria species identification in diagnostic material and cultures
Tatiana Smirnova, Vera Ustinova, Sofya Andreevskaya, Elena Larionova, Ekaterina Kiseleva, Larisa Chernousova, Dmitry Varlamov, Dmitry Sochivko, Atadzhan Ergeshov
Tuberculosis.2021; 130: 102124. CrossRef
Cas12a/Guide RNA-Based Platform for Rapid and Accurate Identification of Major Mycobacterium Species
Guohui Xiao, Xing He, Su Zhang, Yaya Liu, Zhihang Liang, Houming Liu, Juanjuan Zhang, Min Ou, Shuhao Cai, Wenjie Lai, Tianyu Zhang, Lili Ren, Guoliang Zhang, Yi-Wei Tang
Journal of Clinical Microbiology.2020;[Epub] CrossRef
Diagnostic Performance of the GENEDIA MTB/NTM Detection Kit for Detecting Mycobacterium tuberculosis and Nontuberculous Mycobacteria With Sputum Specimens
Sunghwan Shin, In Young Yoo, Hyang Jin Shim, On Kyun Kang, Byung Woo Jhun, Won-Jung Koh, Hee Jae Huh, Nam Yong Lee
Annals of Laboratory Medicine.2020; 40(2): 169. CrossRef
Diagnostic performance of real time PCR and MALDI-TOF in the detection of nontuberculous mycobacteria from clinical isolates
Ellappan Kalaiarasan, Kalpana Thangavelu, Krishnakumariamma Krishnapriya, Muthaiah Muthuraj, Maria Jose, Noyal Mariya Joseph
Tuberculosis.2020; 125: 101988. CrossRef
Comparison of the Three Molecular Diagnostic Assays for Molecular Identification ofMycobacterium tuberculosisand Nontuberculous Mycobacteria Species in Sputum Samples
Jinyoung Bae, Sung-Bae Park, Ji-Hoi Kim, Mi Ran Kang, Kyung Eun Lee, Sunghyun Kim, Hyunwoo Jin
Biomedical Science Letters.2020; 26(3): 170. CrossRef
Rapid Identification of Clinically Relevant Mycobacterium Species by Multicolor Melting Curve Analysis
Ye Xu, Bin Liang, Chen Du, Xueshan Tian, Xingshan Cai, Yanjie Hou, Hui Li, Rongrong Zheng, Junlian Li, Yuqin Liu, Kaili Wang, Muhammad Ammar Athar, Yaoju Tan, Qingge Li, Melissa B. Miller
Journal of Clinical Microbiology.2019;[Epub] CrossRef
Mycobacterium abscessus Complex Cutaneous Infection
Ruben Porudominsky, Eduardo H. Gotuzzo
Current Tropical Medicine Reports.2018; 5(3): 170. CrossRef
The assessment of host and bacterial proteins in sputum from active pulmonary tuberculosis
Hsin-Chih Lai, Yu-Tze Horng, Pen-Fang Yeh, Jann-Yuan Wang, Chin-Chung Shu, Chia-Chen Lu, Jang-Jih Lu, Jen-Jyh Lee, Po-Chi Soo
Journal of Microbiology.2016; 54(11): 761. CrossRef
Efectos adversos de terapia inmunosupresora en paciente reumatológico: infección por micobacterias no tuberculosas
Jean Sebastian Hurtado Hurtado
Reumatología Clínica.2016; 12(2): 118. CrossRef
Adverse Effects of Immunosuppressive Therapy in Rheumatic Patients: Non-tuberculous Mycobacterial Infection
Jean Sebastian Hurtado Hurtado
Reumatología Clínica (English Edition).2016; 12(2): 118. CrossRef
Nontuberculous Mycobacterial Lung Disease Caused byMycobacterium shinjukuense: The First Reported Case in Korea
Seong Mi Moon, Su-Young Kim, Myung Jin Chung, Seung Heon Lee, Sung Jae Shin, Won-Jung Koh
Tuberculosis and Respiratory Diseases.2015; 78(4): 416. CrossRef

Characterization of a Novel Antigen of Mycobacterium tuberculosis K Strain and Its Use in Immunodiagnosis of Tuberculosis: Paul J. Park , Ah Reum Kim , Yangkyo P. Salch , Taeksun Song , Sung Jae Shin , Seung Jung Han , Sang-Nae Cho; J. Microbiol. 2014;52(10):871-878. Published online August 27, 2014; DOI: https://doi.org/10.1007/s12275-014-4235-5

48 View
0 Download
7 Crossref

Abstract

*For correspondence. (S.J. Han) E-mail: hansjung@yuhs.ac / (S.N. Cho) E-mail: raycho@yuhs.ac Paul J. Park, Ah Reum Kim, Yangkyo P. Salch, Taeksun Song, Sung Jae Shin, Seung Jung Han*, and Sang-Nae Cho* Department of Microbiology and Institute for Immunology and Immunological Diseases, Brain Korea 21 Plus Project for the Medical Sciences, Yonsei University College of Medicine, Seoul 120-752, Republic of Korea (Received Apr 16, 2014 / Revised Jul 14, 2014 / Accepted Jul 16, 2014) Journal of Microbiology (2014) Vol. 52, No. 10, pp. 871–878 Copyright 􎨰􀁇2014, The Microbiological Society of Korea DOI 10.1007/s12275-014-4235-5 Characterization of a Novel Antigen of Mycobacterium tuberculosis K strain and Its Use in Immunodiagnosis of Tuberculosis Mycobacterium tuberculosis-specific antigens would be of great value in developing immunodiagnostic tests for tuberculosis (TB), but regional differences in molecular types of the organism may result in antigenic variation, which in turn affects the outcome of the tests. For example, the Beijing strains of M. tuberculosis are prevalent in East Asia, and in particular, the K strain and related strains of the Beijing family, are most frequently isolated during school outbreaks of TB in South Korea. From comparison of genome sequences between M. tuberculosis K strain and the H37Rv strain, a non-Beijing type, we identified a K strain-specific gene, InsB, which has substantial homology with the ESAT-6-like proteins. This study was, therefore, initiated to characterize the InsB protein for its immunogenicity in mice and to confirm its expression in TB patients by detecting antibodies to the protein. The InsB gene was cloned from M. tuberculosis K strain and expressed in Escherichia coli. The recombinant InsB protein was used for immunization of mice. All mice showed strong antibody responses to the InsB protein, and splenocytes stimulated with InsB showed strong IFN-γ and IL-17 responses and a weak IL-2 response, all of which have been implicated in disease expression and used for the immunodiagnosis of TB. Serum samples from TB patients also showed significant antibody responses to the InsB protein as compared to healthy control samples. These results indicate that the InsB protein is an M. tuberculosis K-strain-specific antigen that could further improve the current immunodiagnostic
methods
, especially for the South Korean population.

Citations

Citations to this article as recorded by

Association between Mycobacterium tuberculosis genotype and diabetes mellitus/hypertension: a molecular study
Shengqiong Guo, Shiguang Lei, Prasit Palittapongarnpim, Edward McNeil, Angkana Chaiprasert, Jinlan Li, Huijuan Chen, Weizheng Ou, Komwit Surachat, Wan Qin, Siyu Zhang, Rujuan Luo, Virasakdi Chongsuvivatwong
BMC Infectious Diseases.2022;[Epub] CrossRef
Gradient association between pulmonary tuberculosis and diabetes mellitus among households with a tuberculosis case: a contact tracing-based study
Shengqiong Guo, Shiguang Lei, Jinlan Li, Ling Li, Huijuan Chen, Virasakdi Chongsuvivatwong
Scientific Reports.2022;[Epub] CrossRef
Diagnostic Potential of a PPE Protein Derived fromMycobacterium tuberculosisBeijing/K Strain
Ahreum Kim, Kwang Joo Park, Young Sun Kim, Sang-Nae Cho, Hazel M Dockrell, Yun-Gyoung Hur
Yonsei Medical Journal.2020; 61(9): 789. CrossRef
Immunogenicity and Vaccine Potential of InsB, an ESAT-6-Like Antigen Identified in the Highly Virulent Mycobacterium tuberculosis Beijing K Strain
Woo Sik Kim, Hongmin Kim, Kee Woong Kwon, Sang-Nae Cho, Sung Jae Shin
Frontiers in Microbiology.2019;[Epub] CrossRef
Protective Vaccine Efficacy of the Complete Form of PPE39 Protein from Mycobacterium tuberculosis Beijing/K Strain in Mice
Ahreum Kim, Yun-Gyoung Hur, Sunwha Gu, Sang-Nae Cho, Helene F. Rosenberg
Clinical and Vaccine Immunology.2017;[Epub] CrossRef
Host immune responses to antigens derived from a predominant strain of Mycobacterium tuberculosis
Yun-Gyoung Hur, Wou Young Chung, Ahreum Kim, Young Sun Kim, Hyon-Suk Kim, Sun-Hee Jang, Yeun Kim, Hyeyoung Lee, Kwang Joo Park, Sang-Nae Cho
Journal of Infection.2016; 73(1): 54. CrossRef
Purification and characterization of a novel glycoprotein from Streptomyces sp. ZX01
Guoqiang Zhang, Lirong Han, Guifeng Zhang, Xing Zhang, Juntao Feng
International Journal of Biological Macromolecules.2015; 78: 195. CrossRef

Development and Evaluation of Multiplex Real-time RT-PCR Assays for Seasonal, Pandemic A/H1pdm09 and Avian A/H5 Influenza Viruses Detection: Jang-Hoon Choi , Mi-Seon Kim , Joo-Yeon Lee , Nam-Joo Lee , Donghyok Kwon , Min Gu Kang , Chun Kang; J. Microbiol. 2013;51(2):252-257. Published online April 27, 2013; DOI: https://doi.org/10.1007/s12275-013-2452-y

29 View
0 Download
8 Scopus

Abstract: Since the pandemic influenza A (H1N1) 2009 ((H1N1)pdm09) virus spread all over the world, the (H1N1)pdm09 virus has been circulating with seasonal influenza viruses. We developed rapid and sensitive one-step multiplex real-time RTPCR assays (rRT-PCR) for simultaneous detection of influenza viruses currently circulating in humans, and the avian A/H5 virus. The detection limit of each assay was 4.8 to 1 copies per reaction and no cross-reactivity with other major respiratory pathogens was found. Analytical positive predictive value (PPV), negative predictive value (NPV) sensitivity and specificity were 100%, 94.1%, 93.7% and 100%, respectively. Clinical evaluation revealed that 1,976 (16.5%) of 11,963 throat swabs from patients with respiratory symptoms were confirmed as 1,651 (83.6%) A/H1pdm09, 308 (15.6%) A/H3 and 17 (0.8%) B virus during the 2010-2011 influenza season. Collectively, the multiplex rRT-PCR assays described here provide a practical tool for reliable implementation of influenza surveillance and diagnosis.

PCR-Based Detection of Mycoplasma Species: Hyeran Sung , Seung Hye Kang , Yoon jin Bae , Jin Tae Hong , Youn Bok Chung , Chong-Kil Lee , Sukgil Song; J. Microbiol. 2006;44(1):42-49.; DOI: https://doi.org/2338 [pii]

35 View
0 Download

Abstract: In this study, we describe our newly-developed sensitive two-stage PCR procedure for the detection of 13 common mycoplasmal contaminants (M. arthritidis, M. bovis, M. fermentans, M. genitalium, M. hominis, M. hyorhinis, M. neurolyticum, M. orale, M. pirum, M. pneumoniae, M. pulmonis, M. salivarium, U. urealyticum). For primary amplification, the DNA regions encompassing the 16S and 23S rRNA genes of 13 species were targeted using general mycoplasma primers. The primary PCR products were then subjected to secondary nested PCR, using two different primer pair sets, designed via the multiple alignment of nucleotide sequences obtained from the 13 mycoplasmal species. The nested PCR, which generated DNA fragments of 165-353 bp, was found to be able to detect 1-2 copies of the target DNA, and evidenced no cross-reactivity with the genomic DNA of related microorganisms or of human cell lines, thereby confirming the sensitivity and specificity of the primers used. The identification of contaminated species was achieved via the performance of restriction fragment length polymorphism (RFLP) coupled with Sau3AI digestion. The results obtained in this study furnish evidence suggesting that the employed assay system constitutes an effective tool for the disagnosis of mycoplasmal contamination in cell culture systems.

Review

Shigellosis: Swapan Kumar Niyogi; J. Microbiol. 2005;43(2):133-143.; DOI: https://doi.org/2172 [pii]

35 View
0 Download

Abstract: Shigellosis is a global human health problem. Four species of Shigella i.e. S. dysenteriae, S. flexneri, S. boydii and S. sonnei are able to cause the disease. These species are subdivided into serotypes on the basis of O-specific polysaccharide of the LPS. Shigella dysenteriae type 1 produces severe disease and may be associated with life-threatening complications. The symptoms of shigellosis include diarrhoea and/or dysentery with frequent mucoid bloody stools, abdominal cramps and tenesmus. Shigella spp. cause dysentery by invading the colonic mucosa. Shigella bacteria multiply within colonic epithelial cells, cause cell death and spread laterally to infect and kill adjacent epithelial cells, causing mucosal ulceration, inflammation and bleeding. Transmission usually occurs via contaminated food and water or through person-to-person contact. Laboratory diagnosis is made by culturing the stool samples using selective/differential agar media. Shigella spp. are highly fragile organism and considerable care must be exercised in collecting faecal specimens, transporting them to the laboratories and in using appropriate media for isolation. Antimicrobial agents are the mainstay of therapy of all cases of shigellosis. Due to the global emergence of drug resistance, the choice of antimicrobial agents for treating shigellosis is limited. Although single dose of norfloxacin and ciprofloxacin has been shown to be effective, they are currently less effective against S. dysenteriae type 1 infection. Newer quinolones, cephalosporin derivatives, and azithromycin are the drug of choice. However, fluoroquinolone-resistant S. dysenteriae type 1 infection have been reported. Currently, no vaccines against Shigella infection exist. Both live and subunit parenteral vaccine candidates are under development. Because immunity to Shigella is serotype-specific, the priority is to develop vaccine against S. dysenteriae type 1 and S. flexneri type 2a. Shigella species are important pathogens responsible for diarrhoeal diseases and dysentery occurring all over the world. The morbidity and mortality due to shigellosis are especially high among children in developing countries. A recent review of literature (Kotloff et al.,1999) concluded that, of the estimated 165 million cases of Shigella diarrhoea that occur annually, 99% occur in developing countries, and in developing countries 69% of episodes occur in children under five years of age. Moreover, of the ca.1.1 million deaths attributed to Shigella infections in developing countries, 60% of deaths occur in the under-five age group. Travellers from developed to developing regions and soldiers serving under field conditions are also at an increased risk to develop shigellosis.

Journal Article

Performance of the Immunoglobulin G Avidity and Enzyme Immunoassay IgG/IgM Screening Tests for Differentiation of the Clinical Spectrum of Toxoplasmosis: Mehmet Tanyuksel , Cakir Guney , Engin Araz , M.Ali Saracli , Levent Doganci; J. Microbiol. 2004;42(3):211-215.; DOI: https://doi.org/2087 [pii]

36 View
0 Download

Abstract: Toxoplasmosis has been well known as an important human infection to consider especially in pregnant women. Although many serologic methods are available, the diagnosis of toxoplasmosis can be extremely difficult. The presence of increased levels of Toxoplasma-specific IgG antibodies indicates an infection, but it does not differentiate between a recent and past infection. The purpose of our study was to compare the performance of the ELISA T. gondii IgG/IgM test, a widely used enzyme-linked immunosorbent assay, to the ELISA IgG avidity method. One hundred and four serum samples (from 38 males and 66 females) were tested and evaluated from symptomatic patients (chorioretinitis, lymphadenopathy), and from women in their first trimester of pregnancy who were suspected of having toxoplasmosis. The high IgG avidity and ELISA IgG antibody levels were in agreement for 51 of the specimens (49.0%). Thirty-eight discrepant (borderline) results from the IgG avidity method were positive for IgM (3 specimens) and IgG (37 specimens). Interestingly, out of the eight serum samples that were positive for both IgG and IgM antibodies, two samples were low IgG avidity, and three samples were borderline. There was no statistically significant relation observed between the results of the IgG avidity method and the ELISA IgG test, and the IgG avidity method and ELISA IgM test (c^2=1.987; p=0.370 and c^2=2.152; p=0.341, respectively). The IgG avidity method was considered easy to perform and an acceptable approach for the differentiation of discrepant results (recent/chronic) and for the current detection of T. gondii antibodies. We concluded that the determination of IgG avidity is a helpful tool for the diagnosis of the ocular form of toxoplasmosis and it is a safe method for screening this disease in the first trimester of pregnancy.

The Value of Submitting Multiple Sputum Specimens for Accurate Diagnosis of Pulmonary Tuberculosis: Ozgul Kisa , Ali Albay , Orhan Baylan , Levent Doganci; J. Microbiol. 2002;40(4):301-304.

35 View
0 Download

Abstract: Is a multiple number of sputum specimens necessary for the diagnosis of pulmonary tuberculosis? To answer this question, 6844 respiratory specimens obtained from previously untreated patients suspected of having pulmonary tuberculosis between 1998 and 2001 were evaluated retrospectively. All of the specimens were evaluated by acid fast bacilli smear and BACTEC 460 TB culture system. A total of 785 (11%) specimens from 353 patients were positive for Mycobacterium tuberculosis complex. For 76% (270/353) of these patients the organism was detected from sputum specimens collected sequentially for daily basis. Mycobacterium tuberculosis was isolated in the first, second and third samples of the majority (98%, 195/199) of patients who had three or more sputum samples sent to the laboratory. Our results indicate that, we could carry out Mycobacterium tuberculosis isolation in the first, second and third sputum samples of the overwhelming majority of the patients and the diagnostic value of four or more sputum specimens submitted to the laboratory was very low (2%). We recommend that, for definitive and cost-effective diagnosis of pulmonary tuberculosis at least three sequential sputum specimens be collected for all patients suspected pulmonary tuberculosis.

Laboratory Diagnosis of Invasive Candidiasis: Arjuna N.B. Ellepola , Christine J. Morrison; J. Microbiol. 2005;43(1):65-84.

35 View
0 Download

Abstract: Invasive candidiasis is associated with high morbidity and mortality. Clinical diagnosis is complicated by a lack of specific clinical signs and symptoms of disease. Laboratory diagnosis is also complex because circulating antibodies to Candida species may occur in normal individuals as the result of commensal colonization of mucosal surfaces thereby reducing the usefulness of antibody detection for the diagnosis of this disease. In addition, Candida species antigens are often rapidly cleared from the circulation so that antigen detection tests often lack the desired level of sensitivity. Microbiological confirmation is difficult because blood cultures can be negative in up to 50% of autopsy-proven cases of deep-seated candidiasis or may only become positive late in the infection. Positive cultures from urine or mucosal surfaces do not necessarily indicate invasive disease although can occur during systemic infection. Furthermore, differences in the virulence and in the susceptibility of the various Candida species to antifungal drugs make identification to the species level important for clinical management. Newer molecular biological tests have generated interest but are not yet standardized or readily available in most clinical laboratory settings nor have they been validated in large clinical trials. Laboratory surveillance of at-risk patients could result in earlier initiation of antifungal therapy if sensitive and specific diagnostic tests, which are also cost effective, become available. This review will compare diagnostic tests currently in use as well as those under development by describing their assets and limitations for the diagnosis of invasive candidiasis. <br><br><br>

First
Prev
Page of 1
Next
Last

Search