Journal Articles
- Functional analysis of ascP in Aeromonas veronii TH0426 reveals a key role in the regulation of virulence
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Yongchao Guan , Meng Zhang , Yingda Wang , Zhongzhuo Liu , Zelin Zhao , Hong Wang , Dingjie An , Aidong Qian , Yuanhuan Kang , Wuwen Sun , Xiaofeng Shan
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J. Microbiol. 2022;60(12):1153-1161. Published online November 10, 2022
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DOI: https://doi.org/10.1007/s12275-022-2373-8
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Abstract
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Aeromonas veronii is a pathogen which can induce diseases in
humans, animals and aquatic organisms, but its pathogenic
mechanism and virulence factors are still elusive. In this study,
we successfully constructed a mutant strain (ΔascP) by homologous
recombination. The results showed that the deletion
of the ascP gene significantly down-regulated the expression
of associated effector proteins in A. veronii compared
to its wild type. The adhesive and invasive abilities of ΔascP to
EPC cells were 0.82-fold lower in contrast to the wild strain.
The toxicity of ΔascP to cells was decreased by about 2.91-fold
(1 h) and 1.74-fold (2 h). Furthermore, the LD50 of the mutant
strain of crucian carp was reduced by 19.94-fold, and
the virulence was considerably attenuated. In contrast to the
wild strain, the ΔascP content in the liver and spleen was considerably
lower. The titers of serum cytokines (IL-8, TNF-α,
and IL-1β) in crucian carp after the infection of the ΔascP strain
were considerably lower in contrast to the wild strain. Hence,
the ascP gene is essential for the etiopathogenesis of A. veronii
TH0426.
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- Complete genome sequence and genome-wide transposon mutagenesis enable the determination of genes required for sodium hypochlorite tolerance and drug resistance in pathogen Aeromonas veronii GD2019
Yifan Bu, Chengyu Liu, Yabo Liu, Wensong Yu, Tingjin Lv, Yuanxing Zhang, Qiyao Wang, Yue Ma, Shuai Shao
Microbiological Research.2024; 284: 127731. CrossRef - Construction of the flagellin F mutant of Vibrio parahaemolyticus and its toxic effects on silver pomfret (Pampus argenteus) cells
Yang Li, Chao Liu, Yuechen Sun, Ruijun Wang, Choufei Wu, Hanqu Zhao, Liqin Zhang, Dawei Song, Quanxin Gao
International Journal of Biological Macromolecules.2024; 259: 129395. CrossRef - Ferric uptake regulator (fur) affects the pathogenicity of Aeromonas veronii TH0426 by regulating flagellar assembly and biofilm formation
Jin-shuo Gong, Ying-da Wang, Yan-long Jiang, Di Zhang, Ya-nan Cai, Xiao-feng Shan, He Gong, Hao Dong
Aquaculture.2024; 580: 740361. CrossRef
- The synergy effect of arbuscular mycorrhizal fungi symbiosis and exogenous calcium on bacterial community composition and growth performance of peanut (Arachis hypogaea L.) in saline alkali soil
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Dunwei Ci , Zhaohui Tang , Hong Ding , Li Cui , Guanchu Zhang , Shangxia Li , Liangxiang Dai , Feifei Qin , Zhimeng Zhang , Jishun Yang , Yang Xu
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J. Microbiol. 2021;59(1):51-63. Published online November 17, 2020
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DOI: https://doi.org/10.1007/s12275-021-0317-3
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Abstract
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Peanut (Arachis hypogaea. L) is an important oil seed crop.
Both arbuscular mycorrhizal fungi (AMF) symbiosis and calcium
(Ca2+) application can ameliorate the impact of saline
soil on peanut production, and the rhizosphere bacterial communities
are also closely correlated with peanut salt tolerance;
however, whether AMF and Ca2+ can withstand high-salinity
through or partially through modulating rhizosphere bacterial
communities is unclear. Here, we used the rhizosphere
bacterial DNA from saline alkali soil treated with AMF and
Ca2+ alone or together to perform high-throughput sequencing
of 16S rRNA genes. Taxonomic analysis revealed that
AMF and Ca2+ treatment increased the abundance of Proteobacteria
and Firmicutes at the phylum level. The nitrogenfixing
bacterium Sphingomonas was the dominant genus in
these soils at the genus level, and the soil invertase and urease
activities were also increased after AMF and Ca2+ treatment,
implying that AMF and Ca2+ effectively improved the living
environment of plants under salt stress. Moreover, AMF combined
with Ca2+ was better than AMF or Ca2+ alone at altering
the bacterial structure and improving peanut growth in saline
alkali soil. Together, AMF and Ca2+ applications are conducive
to peanut salt adaption by regulating the bacterial community
in saline alkali soil.
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- Arbuscular mycorrhizal fungi drive bacterial community assembly in halophyte Suaeda salsa
Fengwei Diao, Bingbing Jia, Junqing Luo, Shengli Ding, Tai Liu, Wei Guo
Microbiological Research.2024; 282: 127657. CrossRef - Functional Characterization of Core and Unique Calcite-Dissolving Bacteria Communities from Peanut Fields
Alan Peper, Carter J. Newton, Sheena Lim, Wendy Zheng, Timothy Brenneman, Li Yang
Phytopathology®.2024; 114(5): 1011. CrossRef - Bacterial community composition of Hungarian salt-affected soils under different land uses
Ravi Kumar Gangwar, András Táncsics, Marianna Makádi, Milán Farkas, Mátyás Cserháti, Erika Michéli, Márta Fuchs, Tamás Szegi
Biologia Futura.2024; 75(3): 339. CrossRef - Peanut production in saline-alkali land of Yellow River Delta: influence of spatiotemporal changes of meteorological conditions and soil properties
Feifei Qin, Zhihong Xin, Jianguo Wang, Jialei Zhang, Jishun Yang, Feng Guo, Zhaohui Tang, Dunwei Ci
BMC Plant Biology.2024;[Epub] CrossRef - Multi-year crop rotation and quicklime application promote stable peanut yield and high nutrient-use efficiency by regulating soil nutrient availability and bacterial/fungal community
Liyu Yang, Caibin Wang, Xinhua He, Haiyan Liang, Qi Wu, Xuewu Sun, Miao Liu, Pu Shen
Frontiers in Microbiology.2024;[Epub] CrossRef - Arbuscular Mycorrhizal Fungi Restored the Saline–Alkali Soil and Promoted the Growth of Peanut Roots
Dunwei Ci, Feifei Qin, Zhaohui Tang, Guanchu Zhang, Jialei Zhang, Tong Si, Jishun Yang, Yang Xu, Tianyi Yu, Manlin Xu, Kang He
Plants.2023; 12(19): 3426. CrossRef - Green manure increases peanut production by shaping the rhizosphere bacterial community and regulating soil metabolites under continuous peanut production systems
Yang Xu, Hong Ding, Guanchu Zhang, Zelun Li, Qing Guo, Hao Feng, Feifei Qin, Liangxiang Dai, Zhimeng Zhang
BMC Plant Biology.2023;[Epub] CrossRef - The multifaceted roles of Arbuscular Mycorrhizal Fungi in peanut responses to salt, drought, and cold stress
Yuexu Liu, Jinhao Lu, Li Cui, Zhaohui Tang, Dunwei Ci, Xiaoxia Zou, Xiaojun Zhang, Xiaona Yu, Yuefu Wang, Tong Si
BMC Plant Biology.2023;[Epub] CrossRef - Advances and future research in ecological stoichiometry under saline-alkali stress
Keyi Zhang, Lei Chang, Guanghui Li, Yuefen Li
Environmental Science and Pollution Research.2023; 30(3): 5475. CrossRef - Arbuscular mycorrhizal fungi increased peanut (Arachis hypogaea L.) yield by changing the rhizosphere microbial community structure in saline-alkali soil
Jia Kang, Wenlong Yang, Shangwu Liu, Ning Xuan, Yahui Shao, Yun Geng, Muhammad Afzal, Yingxin Zhang, Shousong Yue, Rubina Mushtaq, Gao Chen
Frontiers in Microbiology.2023;[Epub] CrossRef - Distribution of microbial communities in seasonally frozen soil layers on the Tibetan Plateau and the driving environmental factors
Xiaojie Wang, Zhiqiang Yu, Guofeng Shen, Hefa Cheng, Shu Tao
Environmental Science and Pollution Research.2023; 30(1): 1919. CrossRef - Effects of phosphogypsum on enzyme activity and microbial community in acid soil
Changan Li, Yonggang Dong, Yun Yi, Juan Tian, Chao Xuan, Yan Wang, Yuanbo Wen, Jianxin Cao
Scientific Reports.2023;[Epub] CrossRef - Microbial keystone taxa drive crop productivity through shifting aboveground-belowground mineral element flows
Jia Lin Wang, Kai Lou Liu, Xue Qiang Zhao, Gui-Feng Gao, Yong Hong Wu, Ren Fang Shen
Science of The Total Environment.2022; 811: 152342. CrossRef - Peanut and cotton intercropping increases productivity and economic returns through regulating plant nutrient accumulation and soil microbial communities
Wei Xie, Kai Zhang, Xiaoying Wang, Xiaoxia Zou, Xiaojun Zhang, Xiaona Yu, Yuefu Wang, Tong Si
BMC Plant Biology.2022;[Epub] CrossRef -
Illumina MiSeq Sequencing Reveals Correlations among Fruit Ingredients, Environmental Factors, and AMF Communities in Three
Lycium Barbarum
Producing Regions of China
Kaili Chen, Gang Huang, Yuekun Li, Xinrui Zhang, Yonghui Lei, Yang Li, Jie Xiong, Yanfei Sun, Kristen M. DeAngelis
Microbiology Spectrum.2022;[Epub] CrossRef - Genome-Wide Identification of Auxin Response Factors in Peanut (Arachis hypogaea L.) and Functional Analysis in Root Morphology
Lu Luo, Qian Wan, Zipeng Yu, Kun Zhang, Xiurong Zhang, Suqing Zhu, Yongshan Wan, Zhaojun Ding, Fengzhen Liu
International Journal of Molecular Sciences.2022; 23(10): 5309. CrossRef - Impact of an arbuscular mycorrhizal fungal inoculum and exogenous methyl jasmonate on the performance of tall fescue under saline-alkali condition
Hui Liu, Huimin Tang, Xiaozhen Ni, Yajie Zhang, Yingchao Wang
Frontiers in Microbiology.2022;[Epub] CrossRef - The resistance of peanut to soil-borne pathogens improved by rhizosphere probiotics under calcium treatment
Wei Zhang, Bo-wen Zhang, Jie-fu Deng, Lin Li, Tu-yong Yi, Yan-yun Hong
BMC Microbiology.2021;[Epub] CrossRef - Arbuscular mycorrhizal fungi alleviate salinity stress in peanut: Evidence from pot‐grown and field experiments
Wenjie Qin, Hengyu Yan, Bingyin Zou, Runze Guo, Dunwei Ci, Zhaohui Tang, Xiaoxia Zou, Xiaojun Zhang, Xiaona Yu, Yuefu Wang, Tong Si
Food and Energy Security.2021;[Epub] CrossRef
Reviews
- REVIEW] Recent paradigm shift in the assembly of bacterial tripartite efflux pumps and the type I secretion system
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Inseong Jo , Jin-Sik Kim , Yongbin Xu , Jaekyung Hyun , Kangseok Lee , Nam-Chul Ha
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J. Microbiol. 2019;57(3):185-194. Published online February 26, 2019
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DOI: https://doi.org/10.1007/s12275-019-8520-1
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9
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Abstract
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Tripartite efflux pumps and the type I secretion system of
Gram-negative bacteria are large protein complexes that span
the entire cell envelope. These complexes expel antibiotics and
other toxic substances or transport protein toxins from bacterial
cells. Elucidating the binary and ternary complex structures
at an atomic resolution are crucial to understanding the
assembly and working mechanism. Recent advances in cryoelectron
microscopy along with the construction of chimeric
proteins drastically shifted the assembly models. In this review,
we describe the current assembly models from a historical
perspective and emphasize the common assembly mechanism
for the assembly of diverse tripartite pumps and type
I secretion systems.
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Citations
Citations to this article as recorded by

- Molecular mechanisms of antibiotic resistance revisited
Elizabeth M. Darby, Eleftheria Trampari, Pauline Siasat, Maria Solsona Gaya, Ilyas Alav, Mark A. Webber, Jessica M. A. Blair
Nature Reviews Microbiology.2023; 21(5): 280. CrossRef - Permeation of Fosfomycin through the Phosphate-Specific Channels OprP and OprO of Pseudomonas aeruginosa
Vinaya Kumar Golla, Claudio Piselli, Ulrich Kleinekathöfer, Roland Benz
The Journal of Physical Chemistry B.2022; 126(7): 1388. CrossRef - Adaptation of the periplasm to maintain spatial constraints essential for cell envelope processes and cell viability
Eric Mandela, Christopher J Stubenrauch, David Ryoo, Hyea Hwang, Eli J Cohen, Von L Torres, Pankaj Deo, Chaille T Webb, Cheng Huang, Ralf B Schittenhelm, Morgan Beeby, JC Gumbart, Trevor Lithgow, Iain D Hay
eLife.2022;[Epub] CrossRef - Structure, Assembly, and Function of Tripartite Efflux and Type 1 Secretion Systems in Gram-Negative Bacteria
Ilyas Alav, Jessica Kobylka, Miriam S. Kuth, Klaas M. Pos, Martin Picard, Jessica M. A. Blair, Vassiliy N. Bavro
Chemical Reviews.2021; 121(9): 5479. CrossRef - Biotechnological applications of type 1 secretion systems
Zohreh Pourhassan N., Sander H.J. Smits, Jung Hoon Ahn, Lutz Schmitt
Biotechnology Advances.2021; 53: 107864. CrossRef - Protein-Protein Interactions in the Cytoplasmic Membrane of Escherichia coli: Influence of the Overexpression of Diverse Transporter-Encoding Genes on the Activities of PTS Sugar Uptake Systems
Mohammad Aboulwafa, Zhongge Zhang, Milton H. Saier Jr.
Microbial Physiology.2020; 30(1-6): 36. CrossRef - Perspectives towards antibiotic resistance: from molecules to population
Joon-Hee Lee
Journal of Microbiology.2019; 57(3): 181. CrossRef
- [Minireview] Antibiotic resistance of pathogenic Acinetobacter species and emerging combination therapy
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Bora Shin , Woojun Park
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J. Microbiol. 2017;55(11):837-849. Published online October 27, 2017
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DOI: https://doi.org/10.1007/s12275-017-7288-4
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50
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Abstract
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The increasing antibiotic resistance of Acinetobacter species
in both natural and hospital environments has become a serious
problem worldwide in recent decades. Because of both
intrinsic and acquired antimicrobial resistance (AMR) against
last-resort antibiotics such as carbapenems, novel therapeutics
are urgently required to treat Acinetobacter-associated infectious
diseases. Among the many pathogenic Acinetobacter
species, A. baumannii has been reported to be resistant to all
classes of antibiotics and contains many AMR genes, such as
blaADC (Acinetobacter-derived cephalosporinase). The AMR
of pathogenic Acinetobacter species is the result of several
different mechanisms, including active efflux pumps, mutations
in antibiotic targets, antibiotic modification, and low
antibiotic membrane permeability. To overcome the limitations
of existing drugs, combination theraphy that can increase
the activity of antibiotics should be considered in the
treatment of Acinetobacter infections. Understanding the
molecular mechanisms behind Acinetobacter AMR resistance
will provide vital information for drug development and
therapeutic strategies using combination treatment. Here,
we summarize the classic mechanisms of Acinetobacter AMR,
along with newly-discovered genetic AMR factors and currently
available antimicrobial adjuvants that can enhance drug
efficacy in the treatment of A. baumannii infections.
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- Disruption of bacterial interactions and community assembly in Babesia-infected Haemaphysalis longicornis following antibiotic treatment
Myriam Kratou, Apolline Maitre, Lianet Abuin-Denis, Elianne Piloto-Sardiñas, Ivan Corona-Guerrero, Ana Laura Cano-Argüelles, Alejandra Wu-Chuang, Timothy Bamgbose, Consuelo Almazan, Juan Mosqueda, Dasiel Obregón, Lourdes Mateos-Hernández, Mourad Ben Said,
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Journal of Microbiology, Immunology and Infection.2024; 57(2): 288. CrossRef - MOLECULAR ANALYSIS OF THE MCR-1 GENE IN PSEUDOMONAS AERUGINOSA AND ACINETOBACTER BAUMANII STRAINS
Ömer Akgül
Ankara Universitesi Eczacilik Fakultesi Dergisi.2024; 48(3): 21. CrossRef - Effect of Phenylalanine–Arginine Beta-Naphthylamide on the Values of Minimum Inhibitory Concentration of Quinolones and Aminoglycosides in Clinical Isolates of Acinetobacter baumannii
Stefany Plasencia-Rebata, Saul Levy-Blitchtein, Juana del Valle-Mendoza, Wilmer Silva-Caso, Isaac Peña-Tuesta, William Vicente Taboada, Fernando Barreda Bolaños, Miguel Angel Aguilar-Luis
Antibiotics.2023; 12(6): 1071. CrossRef - A comprehensive genomic analysis provides insights on the high environmental adaptability of Acinetobacter strains
Yang Zhao, Hua-Mei Wei, Jia-Li Yuan, Lian Xu, Ji-Quan Sun
Frontiers in Microbiology.2023;[Epub] CrossRef - Acinetobacter baumannii in blood-borne and central nervous system infections in intensive care unit children: molecular and genetic characteristics and clinical significance
Zulfirya Z. Sadeeva, Irina E. Novikova, Natalia M. Alyabyeva, Anna V. Lazareva, Tatiana M. Komyagina, Olga V. Karaseva, Marina G. Vershinina, Andrey P. Fisenko
Russian Journal of Infection and Immunity.2023; 13(2): 289. CrossRef - Herbal Products and Their Active Constituents Used Alone and in Combination with Antibiotics against Multidrug-Resistant Bacteria
Anna Herman, Andrzej P. Herman
Planta Medica.2023; 89(02): 168. CrossRef - A Systematic Review of Culture-Based Methods for Monitoring Antibiotic-Resistant Acinetobacter, Aeromonas, and Pseudomonas as Environmentally Relevant Pathogens in Wastewater and Surface Water
Erin G. Milligan, Jeanette Calarco, Benjamin C. Davis, Ishi M. Keenum, Krista Liguori, Amy Pruden, Valerie J. Harwood
Current Environmental Health Reports.2023; 10(2): 154. CrossRef - Gold nanoparticle-DNA aptamer-assisted delivery of antimicrobial peptide effectively inhibits Acinetobacter baumannii infection in mice
Jaeyeong Park, Eunkyoung Shin, Ji-Hyun Yeom, Younkyung Choi, Minju Joo, Minho Lee, Je Hyeong Kim, Jeehyeon Bae, Kangseok Lee
Journal of Microbiology.2022; 60(1): 128. CrossRef - In vitro study to evaluate the antimicrobial activity of various multifunctional cosmetic ingredients and chlorphenesin on bacterial species at risk in the cosmetic industry
Benjamin Youenou, Amandine Chauviat, Chrisse Ngari, Valérie Poulet, Sylvie Nazaret
Journal of Applied Microbiology.2022; 132(2): 933. CrossRef - Profiles of Microbial Community and Antibiotic Resistome in Wild Tick Species
Nana Wei, Jinmiao Lu, Yi Dong, Shibo Li, Jack A. Gilbert
mSystems.2022;[Epub] CrossRef - Conventional and Real-Time PCR Targeting blaOXA Genes as Reliable Methods for a Rapid Detection of Carbapenem-Resistant Acinetobacter baumannii Clinical Strains
Dagmara Depka, Agnieszka Mikucka, Tomasz Bogiel, Mateusz Rzepka, Patryk Zawadka, Eugenia Gospodarek-Komkowska
Antibiotics.2022; 11(4): 455. CrossRef - In vitro synergistic activity of colistin and teicoplanin combination against multidrug-resistant Acinetobacter spp
Osama Mohamed Samy Mohamed Rady, Laila El-Attar, Amira Amine
The Journal of Antibiotics.2022; 75(3): 181. CrossRef -
Biogenic silver nanoparticle (Bio‐AgNP) has an antibacterial effect against carbapenem‐resistant
Acinetobacter baumannii
with synergism and additivity when combined with polymyxin B
Suzane Olachea Allend, Marcelle Oliveira Garcia, Kamila Furtado da Cunha, Déborah Trota Farias de Albernaz, Mirian Elert da Silva, Rodrigo Yudi Ishikame, Luciano Aparecido Panagio, Gerson Nakazaro, Guilherme Fonseca Reis, Daniela Brayer Pereira, Daiane Dr
Journal of Applied Microbiology.2022; 132(2): 1036. CrossRef - RapidResa Polymyxin Acinetobacter NP® Test for Rapid Detection of Polymyxin Resistance in Acinetobacter baumannii
Maxime Bouvier, Mustafa Sadek, Stefano Pomponio, Fernando D’Emidio, Laurent Poirel, Patrice Nordmann
Antibiotics.2021; 10(5): 558. CrossRef - Rapid detection of carbapenemase-producing Pseudomonas spp. using the NitroSpeed-Carba NP test
Mustafa Sadek, Laurent Poirel, Patrice Nordmann
Diagnostic Microbiology and Infectious Disease.2021; 99(3): 115280. CrossRef - Gain and loss of antibiotic resistant genes in multidrug resistant bacteria: One Health perspective
Misung Kim, Jaeeun Park, Mingyeong Kang, Jihye Yang, Woojun Park
Journal of Microbiology.2021; 59(6): 535. CrossRef - OXA-23 and OXA-40 producing carbapenem-resistant Acinetobacter baumannii in Central Illinois
Janak Koirala, Isha Tyagi, Lohitha Guntupalli, Sameena Koirala, Udita Chapagain, Christopher Quarshie, Sami Akram, Vidya Sundareshan, Sajan Koirala, Jerry Lawhorn, Yohei Doi, Michael Olson
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Mustafa Sadek, Camille Tinguely, Laurent Poirel, Patrice Nordmann
European Journal of Clinical Microbiology & Infectious Diseases.2020; 39(9): 1657. CrossRef - Stress responses linked to antimicrobial resistance in Acinetobacter species
Bora Shin, Chulwoo Park, Woojun Park
Applied Microbiology and Biotechnology.2020; 104(4): 1423. CrossRef - Carbapenemases: Transforming Acinetobacter baumannii into a Yet More Dangerous Menace
Maria Soledad Ramirez, Robert A. Bonomo, Marcelo E. Tolmasky
Biomolecules.2020; 10(5): 720. CrossRef - Pharmacokinetics, Safety, and Tolerability of Intravenous Durlobactam and Sulbactam in Subjects with Renal Impairment and Healthy Matched Control Subjects
John O’Donnell, Richard A. Preston, Grigor Mamikonyan, Emily Stone, Robin Isaacs
Antimicrobial Agents and Chemotherapy.2019;[Epub] CrossRef - Efficient Delivery of Antisense Oligonucleotides by an Amphipathic Cell-Penetrating Peptide in Acinetobacter baumannii
Zhou Chen, Dan Nie, Yue Hu, Mingkai Li, Zheng Hou, Xinggang Mao, Xiaoxing Luo, Xiaoyan Xue
Current Drug Delivery.2019; 16(8): 728. CrossRef - Restoring the activity of the antibiotic aztreonam using the polyphenol epigallocatechin gallate (EGCG) against multidrug-resistant clinical isolates of Pseudomonas aeruginosa
Jonathan W. Betts, Michael Hornsey, Paul G. Higgins, Kai Lucassen, Julia Wille, Francisco J. Salguero, Harald Seifert, Roberto M. La Ragione
Journal of Medical Microbiology
.2019; 68(10): 1552. CrossRef - Antibiotic-resistant clones in Gram-negative pathogens: presence of global clones in Korea
Kwan Soo Ko
Journal of Microbiology.2019; 57(3): 195. CrossRef - Alternative fate of glyoxylate during acetate and hexadecane metabolism in Acinetobacter oleivorans DR1
Chulwoo Park, Bora Shin, Woojun Park
Scientific Reports.2019;[Epub] CrossRef - Nationwide surveillance of antimicrobial resistance among clinically important Gram-negative bacteria, with an emphasis on carbapenems and colistin: Results from the Surveillance of Multicenter Antimicrobial Resistance in Taiwan (SMART) in 2018
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Fareeha Hameed, Muhammad Asif Khan, Hafsah Muhammad, Tahir Sarwar, Hazrat Bilal, Tayyab Ur Rehman
Revista da Sociedade Brasileira de Medicina Tropical.2019;[Epub] CrossRef - Identification of factors needed by a clinical isolate of Acinetobacter baumannii to resist antibacterial compounds
Celena M. Gwin, Natalia Prakash, Nathan W. Rigel
BIOS.2019; 90(3): 149. CrossRef - A Resazurin Reduction-Based Assay for Rapid Detection of Polymyxin Resistance in Acinetobacter baumannii and Pseudomonas aeruginosa
Mathilde Lescat, Laurent Poirel, Camille Tinguely, Patrice Nordmann, Nathan A. Ledeboer
Journal of Clinical Microbiology.2019;[Epub] CrossRef - Expansion of antibacterial spectrum of xanthorrhizol against Gram-negatives in combination with PMBN and food-grade antimicrobials
Man Su Kim, Ha-Rim Kim, Haebom Kim, Soo-Keun Choi, Chang-Hwan Kim, Jae-Kwan Hwang, Seung-Hwan Park
Journal of Microbiology.2019; 57(5): 405. CrossRef - Carbapenem-resistant Acinetobacter baumannii in patients with burn injury: A systematic review and meta-analysis
William Gustavo Lima, Geisa Cristina Silva Alves, Cristina Sanches, Simone Odília Antunes Fernandes, Magna Cristina de Paiva
Burns.2019; 45(7): 1495. CrossRef - Performances of the Rapid Polymyxin Acinetobacter and Pseudomonas Tests for Colistin Susceptibility Testing
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Emel Mataracı Kara, Mesut Yılmaz, Berna Özbek Çelik
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Bora Shin, Woojun Park
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Richard R. Watkins
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Keith A. Rodvold, Mark H. Gotfried, Robin D. Isaacs, John P. O'Donnell, Emily Stone
Antimicrobial Agents and Chemotherapy.2018;[Epub] CrossRef
- REVIEW] The development of fluconazole resistance in Candida albicans – an example of microevolution of a fungal pathogen
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Joachim Morschhäuser
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J. Microbiol. 2016;54(3):192-201. Published online February 27, 2016
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DOI: https://doi.org/10.1007/s12275-016-5628-4
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Abstract
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The yeast Candida albicans is a member of the microbiota
in the gastrointestinal and urogenital tracts of most healthy
persons, but it can also cause symptomatic infections, especially
in immunocompromised patients. During the life-long
association with its human host, C. albicans generates genetically
altered variants that are better adapted to changes in
their environment. A prime example of this microevolution
is the development of resistance to the commonly used drug
fluconazole, which inhibits ergosterol biosynthesis, during
antimycotic therapy. Fluconazole resistance can be caused by
mutations in the drug target, by changes in the sterol biosynthesis
pathway, and by gain-of-function mutations in transcription
factors that result in the constitutive upregulation
of ergosterol biosynthesis genes and multidrug efflux pumps.
Fluconazole also induces genomic rearrangements that result
in gene amplification and loss of heterozygosity for resistance
mutations, which further increases drug resistance.
These genome alterations may affect extended chromosomal
regions and have additional phenotypic consequences. A
striking case is the loss of heterozygosity for the mating type
locus MTL in many fluconazole-resistant clinical isolates,
which allows the cells to switch to the mating-competent opaque
phenotype. This, in turn, raises the possibility that sexual
recombination between different variants of an originally clonal,
drug-susceptible population may contribute to the generation
of highly fluconazole-resistant strains with multiple
resistance mechanisms. The gain-of-function mutations in
transcription factors, which result in deregulated gene expression,
also cause reduced fitness. In spite of this, many clinical
isolates that contain such mutations do not exhibit fitness defects,
indicating that they have overcome the costs of drug
resistance with further evolution by still unknown mechanisms.
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Research Support, Non-U.S. Gov't
- Conditional probability analysis of multidrug resistance in Gram-negative bacilli isolated from tertiary medical institutions in South Korea during 1999–2009
-
Yong-Hak Kim
-
J. Microbiol. 2016;54(1):50-56. Published online January 5, 2016
-
DOI: https://doi.org/10.1007/s12275-016-5579-9
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47
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Abstract
-
Multidrug resistance of Gram-negative bacilli is a major problem
globally. However, little is known about the combined
probability of resistance to various antibiotics. In this study,
minimum inhibitory concentrations of widely used antibiotics
were determined using clinical isolates of Escherichia coli,
Pseudomonas aeruginosa, Klebsiella pneumoniae, and Acinetobacter
baumannii, randomly chosen from strain collections
created during 1999–2009 in tertiary medical institutions in
Seoul, South Korea. To analyze combined efficacy of antibiotics
against a subgroup of isolates, conditional probabilities
were determined based on arbitrary, non-independent patterns
of antimicrobial susceptibility and resistance. Multidrug
resistance, defined as resistance to three or more classes of
antibiotics, was observed in the following order: A. baumannii
(96%), P. aeruginosa (65%), E. coli (52%), and K. pneumoniae
(7%). A. baumannii strains resistant to gentamicin were found
to be resistant to a number of antibiotics, except for colistin
and polymyxin B. Resistance to gentamicin following exposure
to this antibiotic was highly likely to lead to multidrug
resistance in all four microbes. This study shows a causal
relationship between gentamicin resistance and the prevalence
of multidrug resistance in clinical isolates of Gramnegative
bacilli in South Korea during 1999–2009 and suggests
the importance of prudent use of gentamicin in hospitals.
-
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DOI: https://doi.org/10.1007/s12275-016-5159-z
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Crossref
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Abstract
-
Antibiotic resistance is rapidly spreading among bacteria
such as Staphylococcus aureus, an opportunistic bacterial
pathogen that causes a variety of diseases in humans. For
the last two decades, bacterial multidrug efflux pumps have
drawn attention due to their potential association with clinical
multidrug resistance. Numerous researchers have demonstrated
efflux-mediated resistance in vitro and in vivo
and found novel multidrug transporters using advanced
genomic information about bacteria. This article aims to
provide a concise summary of multidrug efflux pumps and
their important clinical implications, focusing on recent
findings concerning S. aureus efflux pumps.
-
Citations
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- MINIREVIEW] Molecular architecture of the bacterial tripartite multidrug efflux pump focusing on the adaptor bridging model
-
Saemee Song , Jin-Sik Kim , Kangseok Lee , Nam-Chul Ha
-
J. Microbiol. 2015;53(6):355-364. Published online May 30, 2015
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DOI: https://doi.org/10.1007/s12275-015-5248-4
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Abstract
-
Gram-negative bacteria expel a wide range of toxic substances
through tripartite drug efflux pumps consisting of an inner
membrane transporter, an outer membrane channel protein,
and a periplasmic adaptor protein. These pumps form tripartite
assemblies which can span the entire cell envelope,
including the inner and outer membranes. There have been
controversial findings regarding the assembly of the individual
components in tripartite drug efflux pumps. Recent
structural and functional studies have advanced our understanding
of the assembly and working mechanisms of the
pumps. Here, we re-evaluate the assembly models based on
recent structural and functional studies. In particular, this
study focuses on the ‘adaptor bridging model’, highlighting
the intermeshing cogwheel-like interactions between the tip
regions of the outer membrane channel protein and the periplasmic
adaptor protein in the hexameric assembly.
-
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Nature Microbiology.2017;[Epub] CrossRef - Structural Basis for the Serratia marcescens Lipase Secretion System: Crystal Structures of the Membrane Fusion Protein and Nucleotide-Binding Domain
Daichi Murata, Hiroyuki Okano, Clement Angkawidjaja, Masato Akutsu, Shun-ichi Tanaka, Kenyu Kitahara, Takuya Yoshizawa, Hiroyoshi Matsumura, Yuji Kado, Eiichi Mizohata, Tsuyoshi Inoue, Satoshi Sano, Yuichi Koga, Shigenori Kanaya, Kazufumi Takano
Biochemistry.2017; 56(47): 6281. CrossRef - The Crystal Structure of the YknZ Extracellular Domain of ABC Transporter YknWXYZ from Bacillus amyloliquefaciens
Yongbin Xu, Jianyun Guo, Lulu Wang, Rui Jiang, Xiaoling Jin, Jing Liu, Shengdi Fan, Chun-Shan Quan, Nam-Chul Ha, Bostjan Kobe
PLOS ONE.2016; 11(5): e0155846. CrossRef
Research Support, Non-U.S. Gov'ts
- Functional analysis of Vibrio vulnificus RND efflux pumps homologous to Vibrio cholerae VexAB and VexCD, and to Escherichia coli AcrAB
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Seunghwa Lee , Ji-Hyun Yeom , Sojin Seo , Minho Lee , Sarang Kim , Jeehyeon Bae , Kangseok Lee , Jihwan Hwang
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J. Microbiol. 2015;53(4):256-261. Published online March 4, 2015
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DOI: https://doi.org/10.1007/s12275-015-5037-0
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56
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9
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Abstract
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Resistance-nodulation-division (RND) efflux pumps are associated
with multidrug resistance in many gram-negative
pathogens. The genome of Vibrio vulnificus encodes 11 putative
RND pumps homologous to those of Vibrio cholerae
and Escherichia coli. In this study, we analyzed three putative
RND efflux pumps, showing homology to V. cholerae VexAB
and VexCD and to E. coli AcrAB, for their functional roles in
multidrug resistance of V. vulnificus. Deletion of the vexAB
homolog resulted in increased susceptibility of V. vulnificus
to bile acid, acriflavine, ethidium bromide, and erythromycin,
whereas deletion of acrAB homologs rendered V. vulnificus
more susceptible to acriflavine only. Deletion of vexCD had
no effect on susceptibility of V. vulnificus to these chemicals.
Upon exposure to these antibacterial chemicals, expression
of tolCV1 and tolCV2, which are putative outer membrane
factors of RND efflux pumps, was induced, whereas expression
levels of vexAB, vexCD, and acrAB homologs were not
significantly changed. Our results show that the V. vulnificus
homologs of VexAB largely contributed to in vitro antimicrobial
resistance with a broad substrate specificity that
was partially redundant with the AcrAB pump homologs.
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Citations
Citations to this article as recorded by

- Dynamics of efflux pumps in antimicrobial resistance, persistence, and community living of Vibrionaceae
Sanath Kumar, Manjusha Lekshmi, Jerusha Stephen, Anely Ortiz-Alegria, Matthew Ayitah, Manuel F. Varela
Archives of Microbiology.2024;[Epub] CrossRef - Membrane Efflux Pumps of Pathogenic Vibrio Species: Role in Antimicrobial Resistance and Virulence
Jerusha Stephen, Manjusha Lekshmi, Parvathi Ammini, Sanath H. Kumar, Manuel F. Varela
Microorganisms.2022; 10(2): 382. CrossRef - TolCV1 Has Multifaceted Roles During Vibrio vulnificus Infection
Yue Gong, Rui Hong Guo, Joon Haeng Rhee, Young Ran Kim
Frontiers in Cellular and Infection Microbiology.2021;[Epub] CrossRef - The mechanisms that regulate Vibrio parahaemolyticus virulence gene expression differ between pathotypes
Nicholas Petronella, Jennifer Ronholm
Microbial Genomics
.2018;[Epub] CrossRef - ATP-Binding Cassette Transporter VcaM from Vibrio cholerae is Dependent on the Outer Membrane Factor Family for Its Function
Wen-Jung Lu, Hsuan-Ju Lin, Thamarai Janganan, Cheng-Yi Li, Wei-Chiang Chin, Vassiliy Bavro, Hong-Ting Lin
International Journal of Molecular Sciences.2018; 19(4): 1000. CrossRef - Contribution of efflux systems to the detergent resistance, cytotoxicity, and biofilm formation of Vibrio vulnificus
Chung-Cheng Lo, Pei-Ting Lin, Chuan Chiang-Ni, Kuan-Hua Lin, Shin-Luen Lee, Tzu-Fang Kuo, Horng-Ren Lo
Gene Reports.2017; 9: 115. CrossRef - Survival of the Fittest: How Bacterial Pathogens Utilize Bile To Enhance Infection
Jeticia R. Sistrunk, Kourtney P. Nickerson, Rachael B. Chanin, David A. Rasko, Christina S. Faherty
Clinical Microbiology Reviews.2016; 29(4): 819. CrossRef - MdsABC-Mediated Pathway for Pathogenicity in Salmonella enterica Serovar Typhimurium
Saemee Song, Boeun Lee, Ji-Hyun Yeom, Soonhye Hwang, Ilnam Kang, Jang-Cheon Cho, Nam-Chul Ha, Jeehyeon Bae, Kangseok Lee, Yong-Hak Kim, S. M. Payne
Infection and Immunity.2015; 83(11): 4266. CrossRef - Molecular architecture of the bacterial tripartite multidrug efflux pump focusing on the adaptor bridging model
Saemee Song, Jin-Sik Kim, Kangseok Lee, Nam-Chul Ha
Journal of Microbiology.2015; 53(6): 355. CrossRef
- Frequency of Antibiotic Resistance in Helicobacter pylori Strains Isolated from the Northern Population of Iran
-
Amin Talebi Bezmin Abadi , Tarang Taghvaei , Ashraf Mohabbati Mobarez , Beth M. Carpenter , D. Scott Merrell
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J. Microbiol. 2011;49(6):987-993. Published online December 28, 2011
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DOI: https://doi.org/10.1007/s12275-011-1170-6
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29
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42
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Abstract
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The purpose of this study was to evaluate the primary resistance rates of recent clinical Helicobacter pylori isolates to the most commonly used antibiotics in Iran. Two hundreds and ten patients presenting with gastric maladies between January and July of 2009 were enrolled in this study. Endoscopy was performed, and biopsy specimens were collected from each patient for subsequent bacterial culture of H. pylori. Single colony isolates from each patient were then used for antimicrobial susceptibility testing. The disk diffusion method was used to determine susceptibility patterns. One hundred and ninety-seven of the patients were H. pylori positive (93.8%). The rates of resistance to tetracycline, amoxicillin, ciprofloxacin, metronidazole, clarithromycin, and furizoladone were 37.1%, 23.9%, 34.5%, 65.5%, 45.2%, and 61.4%, respectively. A significant association between amoxicillin resistance and disease state (P<0.05) was identified. Furthermore, some double, triple, quadruple, and quintuple combinations of antibiotic resistance were found to be associated with disease state. This study evaluated the prevalence of H. pylori resistance to the most commonly prescribed antibiotics used in Iran and showed that resistance rates were generally higher than previously reported. This data adds to the growing body of evidence that suggests there is increasing antibiotic resistance among H. pylori isolates, which likely is responsible for the decreasing efficacy of anti-H. pylori therapy at the local and global level. Hence, there is a need for continued monitoring of resistance patterns, especially at the local level, and for incorporation of that information into treatment regimens for H. pylori infections.
- Occurrence and Antimicrobial Drug Susceptibility Patterns of Commensal and Diarrheagenic Escherichia coli in Fecal Microbiota from Children with and without Acute Diarrhea
-
Patrícia G. Garcia , Vânia L. Silva , Cláudio G. Diniz
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J. Microbiol. 2011;49(1):46-52. Published online March 3, 2011
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DOI: https://doi.org/10.1007/s12275-011-0172-8
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39
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36
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Abstract
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Acute diarrhea is a public health problem and an important cause of morbidity and mortality, especially in developing countries. The etiology is varied, and the diarrheagenic Escherichia coli pathotypes are among the most important. Our objectives were to determine the occurrence of commensal and diarrheagenic E.
coli strains in fecal samples from children under five years old and their drug susceptibility patterns. E. coli were isolated from 141 fresh fecal samples; 84 were obtained from clinically injured donors with acute diarrhea (AD) and 57 from clinically healthy donors without diarrhea (WD). Presumptive phenotypic species
identification was carried out and confirmed by amplification of specific 16S ribosomal RNA encoding DNA. Multiplex PCR was performed to characterize the diarrheagenic E. coli strains. Drug susceptibility patterns were determined by the disc-diffusion method. In total, 220 strains were recovered from the fecal specimens
(61.8% from AD and 38.2% from WD). Diarrheagenic E. coli was identified at a rate of 36.8% (n=50) in diarrheic feces and 29.8% (n=25) in non-diarrheic feces. Enteroaggregative E. coli was the most frequently identified pathotype in the AD group (16.2%) and the only pathotype identified in the WD group (30.9%).
Enteropathogenic E. coli was the second most isolated pathotype (10.3%), followed by Shiga toxin-producing E. coli (7.4%) and enterotoxigenic E. coli (2.9%). No enteroinvasive E. coli strains were recovered. The isolates showed high resistance rates against ampicillin, tetracycline, and sulfamethoxazole-trimethoprim.
The most effective drugs were ceftazidime, ceftriaxone, imipenem and piperacillin-tazobactam, for which no resistance was observed. Differentiation between the diarrheagenic E. coli pathotypes is of great importance since they are involved in acute diarrheal diseases and may require specific antimicrobial chemotherapy. The high antimicrobial resistance observed in our study raises a broad discussion on the
indiscriminate or improper use of antimicrobials, besides the risks of self-medication.
-
Citations
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BMC Infectious Diseases.2016;[Epub] CrossRef - Passive immunisation, an old idea revisited: Basic principles and application to modern animal production systems
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Yong Huang, Xue-feng Shan, Haijun Deng, Yu-jun Huang, Xiao-ping Mu, Ai-long Huang, Quan-xin Long
Japanese Journal of Infectious Diseases.2015; 68(3): 239. CrossRef - Diarrhoeagenic Escherichia coli in mother-child Pairs in Ile-Ife, South Western Nigeria
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BMC Infectious Diseases.2015;[Epub] CrossRef - Genetic Relatedness Among Escherichia coli Pathotypes Isolated from Food Products for Human Consumption in Cartagena, Colombia
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- The Diversity of Multi-drug Resistance Profiles in Tetracycline-Resistant Vibrio Species Isolated from Coastal Sediments and Seawater
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Farzana Ashrafi Neela , Lisa Nonaka , Satoru Suzuki
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J. Microbiol. 2007;45(1):64-68.
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DOI: https://doi.org/2489 [pii]
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Abstract
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In this study we examined the multi-drug resistance profiles of the tetracycline (TC) resistant genus Vibrio to determine its susceptibility to two β-lactams, ampicillin (ABPC), and mecillinam (MPC), as well as to macrolide, erythromycin (EM). The results showed various patterns of resistance among strains that were isolated from very close geographical areas during the same year, suggesting diverse patterns of drug resistance in environmental bacteria from this area. In addition, the cross-resistance patterns suggested that the resistance determinants among Vibrio spp. are acquired differently within the sediment and seawater environments.
Review
- Shigellosis
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Swapan Kumar Niyogi
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J. Microbiol. 2005;43(2):133-143.
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DOI: https://doi.org/2172 [pii]
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Abstract
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Shigellosis is a global human health problem. Four species of Shigella i.e. S. dysenteriae, S. flexneri, S. boydii and S. sonnei are able to cause the disease. These species are subdivided into serotypes on the basis of O-specific polysaccharide of the LPS. Shigella dysenteriae type 1 produces severe disease and may be associated with life-threatening complications. The symptoms of shigellosis include diarrhoea and/or dysentery with frequent mucoid bloody stools, abdominal cramps and tenesmus. Shigella spp. cause dysentery by invading the colonic mucosa. Shigella bacteria multiply within colonic epithelial cells, cause cell death and spread laterally to infect and kill adjacent epithelial cells, causing mucosal ulceration, inflammation and bleeding. Transmission usually occurs via contaminated food and water or through person-to-person contact. Laboratory diagnosis is made by culturing the stool samples using selective/differential agar media. Shigella spp. are highly fragile organism and considerable care must be exercised in collecting faecal specimens, transporting them to the laboratories and in using appropriate media for isolation. Antimicrobial agents are the mainstay of therapy of all cases of shigellosis. Due to the global emergence of drug resistance, the choice of antimicrobial agents for treating shigellosis is limited. Although single dose of norfloxacin and ciprofloxacin has been shown to be effective, they are currently less effective against S. dysenteriae type 1 infection. Newer quinolones, cephalosporin derivatives, and azithromycin are the drug of choice. However, fluoroquinolone-resistant S. dysenteriae type 1 infection have been reported. Currently, no vaccines against Shigella infection exist. Both live and subunit parenteral vaccine candidates are under development. Because immunity to Shigella is serotype-specific, the priority is to develop vaccine against S. dysenteriae type 1 and S. flexneri type 2a.
Shigella species are important pathogens responsible for diarrhoeal diseases and dysentery occurring all over the world. The morbidity and mortality due to shigellosis are especially high among children in developing countries. A recent review of literature (Kotloff et al.,1999) concluded that, of the estimated 165 million cases of Shigella diarrhoea that occur annually, 99% occur in developing countries, and in developing countries 69% of episodes occur in children under five years of age. Moreover, of the ca.1.1 million deaths attributed to Shigella infections in developing countries, 60% of deaths occur in the under-five age group. Travellers from developed to developing regions and soldiers serving under field conditions are also at an increased risk to develop shigellosis.
Research Support, Non-U.S. Gov't
- Isolation of Quinupristin/Dalfopristin-Resistant Streptococcus agalactiae from Asymptomatic Korean Women
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Hye Ran Nam , Hak Mee Lee , Yeonhee Lee
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J. Microbiol. 2008;46(1):108-111.
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DOI: https://doi.org/10.1007/s12275-007-0217-1
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27
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1
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Abstract
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Seven Streptococcus agalactiae isolates were obtained from the vagina of 80 asymptomatic women. Three of these isolates showed multi-drug resistant (MDR) phenotypes: two isolates were resistant to clarithromycin, clindamycin, erythromycin, and tetracycline; and one isolate was resistant to clarithromycin, clindamycin, erythromycin, tetracycline, and quinupristin/dalfopristin. There was no clonal relationship among the MDR isolates. This is the first report of quinupristin/dalfopristin-resistant S. agalactiae.
- Genetic Relatedness within Streptococcus pneumoniae Serotype 19F and 23F Isolates in Korea by Pulsed-Field Gel Electrophoresis
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Kwang Jun Lee , Song Mee Bae , Kyu Jam Hwang , Young Hee Lee , Ki Sang Kim
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J. Microbiol. 2003;41(1):1-6.
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Abstract
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The genetic relatedness of multidrug-resistant pneumococcal isolates of serotypes 19F and 23F was investigated. The DNA fragments digested with Sma I were resolved by pulsed-field gel electrophoresis (PFGE). PFGE analysis of 36 S. pneumoniae isolates showed 13 different patterns. Among 22 isolates of serotype 19F, 9 different PFGE patterns were present and 14 isolates of serotype 23F isolates represented 5 distinct PFGE patterns. Two isolates of serotype 19F and six isolates of serotype 23F shared the same PFGE pattern (Pattern I). Based on the genetic relatedness within the strains (one genetic cluster was defined as having more than 85% homology), we divided the pneumococcal strains into 6 genetic clusters (I, II, III, IV, V, and VI). The 22 strains of serotype 19F belonged to five distinct genetic clusters (I, II, III, IV, V and VI) and 14 strains of serotype 23F represented two genetic clusters (I and II ). These results showed that strains of serotype 19F are genetically more diverse than those of serotype 23F. Serotype 19F isolates with PFGE patterns H and I appeared to be less related to those of the remaining PFGE patterns (A to G) (less than 60% genetic relatedness), but those strains were genetically closely related with serotype 23F. These results suggest that the latter isolates originated from horizontal transfer of the capsular type 19F gene locus to 23F pneumococcal genotypes. In conclusion, the multidrug-resistant pneumococcal isolates of serotype 19F and 23F isolated in Korea are the result of the spread of a limited number of resistant clones.