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- Identification of cyst wall proteins of the hypotrich ciliate Euplotes encysticus using a proteomics approach
- Bangzheng Wang , Tao Niu , Muhammad Zeeshan Bhatti , Fenfen Chen , Lin Wu , Jiwu Chen
- J. Microbiol. 2017;55(7):545-553. Published online June 30, 2017
- DOI: https://doi.org/10.1007/s12275-017-6422-7
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- 11 Crossref
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Abstract
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Euplotes encysticus is a species of Hypotrich ciliates, which
form cyst wall by secreting the special substances on encounter
of adverse environment. It has critical significance to study
the component and mechanism underlying resting cyst, during
resisting unfavorable conditions in dormancy induction.
The present study was aimed to investigate the effects of cyst
wall proteins of Euplotes encysticus by using biochemical
methods
. Therefore, protein extracts were separated by SDSPAGE, identified and analyzed by MALDI-TOF MS and Bioinformatics tools. We detected 42 cyst wall proteins, 26 were functional proteins and 16 proteins consist of unknown function; which is consistent with cyst wall specificity. These results partially revealed the components of resting cyst wall formed after the cells differentiation of Euplotes encysticus. In addition, our data suggested that the function of cyst wall proteins are more likely involved in the mechanical protection, signal transduction, material transport, protein degradation and energy metabolism to survival, with potentially importance implications in the molecular mechanism of eukaryocyte dormancy under stress condition. -
Citations
Citations to this article as recorded by
- Decryption of the survival “black box”: gene family expansion promotes the encystment in ciliated protists
Didi Jin, Chao Li, Xiao Chen, Yurui Wang, Khaled A. S. Al-Rasheid, Naomi A. Stover, Chen Shao, Tengteng Zhang
BMC Genomics.2024;[Epub] CrossRef - Role of chitin synthases CHS1 and CHS2 in biosynthesis of the cyst wall of Cryptocaryon irritans
Huicheng Wu, Yihao Cen, Yipei Lu, Pengbo Dan, Yanwei Li, Xueming Dan, Zequan Mo
International Journal of Biological Macromolecules.2024; 280: 136143. CrossRef - Water temperature affects Cryptocaryon irritans development, cryptocaryoniasis occurrence and an auxiliary treatment decision-making webpage
Fei Yin, Jing Yin, Xiao Xie, Linhua Jiang
Aquaculture.2023; 574: 739694. CrossRef - A light and electron microscopical study on the resting cyst of the tintinnid Schmidingerella (Alveolata, Ciliophora) including a phylogeny-aware comparison
Maximilian H. Ganser, Heidi Bartel, Birgit Weißenbacher, Ancuela Andosch, Ursula Lütz-Meindl, Peter Radacher, Sabine Agatha
European Journal of Protistology.2022; 86: 125922. CrossRef - How Ciliated Protists Survive by Cysts: Some Key Points During Encystment and Excystment
Yuqing Li, Yurui Wang, Shijing Zhang, Xyrus X. Maurer-Alcalá, Ying Yan
Frontiers in Microbiology.2022;[Epub] CrossRef - Early signaling pathways mediating dormant cyst formation in terrestrial unicellular eukaryoteColpoda
Tatsuomi Matsuoka
FEMS Microbiology Letters.2021;[Epub] CrossRef - Novel insights into molecular mechanisms of Pseudourostyla cristata encystment using comparative transcriptomics
Nan Pan, Tao Niu, Muhammad Zeeshan Bhatti, Haiyang Zhang, Xinpeng Fan, Bing Ni, Jiwu Chen
Scientific Reports.2019;[Epub] CrossRef - New contribution to the morphology and molecular mechanism of Euplotes encysticus encystment
Fenfen Chen, Yanyan Xue, Nan Pan, Muhammad Zeeshan Bhatti, Tao Niu, Jiwu Chen
Scientific Reports.2018;[Epub] CrossRef - Morphological and Molecular Redefinition of Euplotes platystoma Dragesco & Dragesco‐Kernéis, 1986 and Aspidisca lynceus (Müller,1773) Ehrenberg, 1859, with Reconsideration of a “Well‐known” Euplotes Ciliate, Euplotes harpa Stein, 1859 (Ciliophora, Euploti
Chunyu Lian, Xiaotian Luo, Xinpeng Fan, Jie Huang, Yuhe Yu, William Bourland, Weibo Song
Journal of Eukaryotic Microbiology.2018; 65(4): 531. CrossRef - The affiliation of Hexasterias problematica and Halodinium verrucatum sp. nov. to ciliate cysts based on molecular phylogeny and cyst wall composition
Pieter R. Gurdebeke, Kenneth Neil Mertens, Yoshihito Takano, Aika Yamaguchi, Kara Bogus, Micah Dunthorn, Kazumi Matsuoka, Henk Vrielinck, Stephen Louwye
European Journal of Protistology.2018; 66: 115. CrossRef - Ultrastructure of vegetative cells and resting cysts, and live observations of the encystation and excystation processes in Diophrys oligothrix Borror, 1965 (Protista, Ciliophora)
Zhi‐Wei Gong, Xin‐Peng Fan, Rui Ma, Bing Ni
Journal of Morphology.2018; 279(10): 1397. CrossRef
- Decryption of the survival “black box”: gene family expansion promotes the encystment in ciliated protists
- Ultrastructural studies of encystment in allomyces macrogynus
- Kim, Jung Soeup , Youn, Hyun Joo , Cho, Chung Won
- J. Microbiol. 1996;34(3):220-224.
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Abstract
- Ultrastructural organization of encysting zoospores of Allomyces macrogynus was examined using the methods of cryofixation and freeze substitution. During enxystment, obvious changes were observed at the surface of the plasma membrane and in the structure of gamma particles. Many multivesicular bodies associated with the plasma membrane were observed at early stages of encystment. After induction of encystment, vesicles were found within the gamma particles. These vesicles appeared to leave gamma particles after formaing multivesicular bodies. This study suggests that the cell wall formation during encystment is mediated by the fusion of multivesicular bodies with the plasma membrane.
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