Review
- Minireivew] Protective Role of Gut Commensal Microbes against Intestinal Infections
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My Young Yoon , Keehoon Lee , Sang Sun Yoon
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J. Microbiol. 2014;52(12):983-989. Published online November 29, 2014
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DOI: https://doi.org/10.1007/s12275-014-4655-2
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Abstract
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The human gastrointestinal tract is colonized by multitudes
of microorganisms that exert beneficial effects on human
health. Mounting evidence suggests that intestinal microbiota
contributes to host resistance against enteropathogenic
bacterial infection. However, molecular details that account
for such an important role has just begun to be understood.
The commensal microbes in the intestine regulate gut homeostasis
through activating the development of host innate
immunity and producing molecules with antimicrobial activities
that directly inhibit propagation of pathogenic bacteria.
Understanding the protective roles of gut microbiota
will provide a better insight into the molecular basis that underlies
complicated interaction among host-pathogen-symbiont.
In this review, we highlighted recent findings that help
us broaden our knowledge of the intestinal ecosystem and
thereby come up with a better strategy for combating enteropathogenic
infection.
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Citations
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Research Support, Non-U.S. Gov't
- Detection of Representative Enteropathogenic Bacteria, Vibrio spp., Pathogenic Escherichia coli, Salmonella spp., Shigella spp., and Yersinia enterocolitica, Using a Virulence Factor Gene-Based Oligonucleotide Microarray§
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Dong-Hun Kim , Bok-Kwon Lee , Yong-Dae Kim , Sung-Keun Rhee , Young-Chang Kim
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J. Microbiol. 2010;48(5):682-688. Published online November 3, 2010
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DOI: https://doi.org/10.1007/s12275-010-0119-5
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Scopus
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Abstract
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Rapid identification of enteropathogenic bacteria in stool samples is critical for clinical diagnosis and antimicrobial therapy. In this study, we describe the development of an approach that couples multiplex PCR with hybridization to a DNA microarray, to allow the simultaneous detection of the 10 pathogens. The microarray was synthesized with 20-mer oligonucleotide probes that were designed to be specific for virulencefactor genes of each strain. The detection limit for genomic DNA from a single strain was approximately 10 fg. In the presence of heterogeneous non-target DNA, the detection sensitivity of the array decreased to approximately 100 fg. We did not observe any non-specific hybridization. In addition, we successfully used this oligonucleotide-based DNA microarray to identify the causative agents in clinical stool samples from patients with food-borne enteritis.