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- Detailed Modes of Action and Biochemical Characterization of endo-Arabinanase from Bacillus licheniformis DSM13
- Jung-Mi Park , Myoung-Uoon Jang , Jung-Hyun Kang , Min-Jeong Kim , So-Won Lee , Yeong Bok Song , Chul-Soo Shin , Nam Soo Han , Tae-Jip Kim
- J. Microbiol. 2012;50(6):1041-1046. Published online December 30, 2012
- DOI: https://doi.org/10.1007/s12275-012-2489-3
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Abstract
- An endo-arabinanase (BLABNase) gene from Bacillus licheniformis DSM13 was cloned and expressed in Escherichia coli, and the biochemical properties of its encoded enzyme were characterized. The BLABNase gene consists of a single open reading frame of 987 nucleotides that encodes 328 amino acids with a predicted molecular mass of about 36 kDa. BLABNase exhibited the highest activity against debranched α-(1,5)-arabinan in 50 mM sodium acetate buffer (pH 6.0) at 55°C. Enzymatic characterization revealed that BLABNase hydrolyzes debranched or linear arabinans with a much higher activity than branched arabinan from sugar beet. Enzymatic hydrolysis pattern analyses demonstrated BLABNase to be a typical endo-(1,5)-α-L-arabinanase (EC 3.2.1.99) that randomly cleaves the internal α-(1,5)-linked L-arabinofuranosyl residues of a branchless arabinan backbone to release arabinotriose mainly, although a small amount of arabino-oligosaccharide intermediates is also liberated. Our results indicated that BLABNase acts preferentially along with the oligosaccharides longer than arabinopentaose, thus enabling the enzymatic production of various arabinooligosaccharides.
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Citations
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Carbohydrate Polymers.2015; 131: 50. CrossRef - Increased biomass saccharification by supplementation of a commercial enzyme cocktail with endo-arabinanase from Bacillus licheniformis
Carla Botelho Machado, Ana Paula Citadini, Rosana Goldbeck, Evandro Antônio de Lima, Fernanda Lopes Figueiredo, Tony Márcio da Silva, Zaira Bruna Hoffmam, Amanda Silva de Sousa, Fábio Márcio Squina, Maria de Lourdes Teixeira de Moraes Poliz, Roberto Rulle
Biotechnology Letters.2015; 37(7): 1455. CrossRef - Biochemical Characterization of a Novel Endo-1,5-α-l-arabinanase fromRhizomucor miehei
Zhou Chen, Yu Liu, Qiaojuan Yan, Shaoqing Yang, Zhengqiang Jiang
Journal of Agricultural and Food Chemistry.2015; 63(4): 1226. CrossRef
- Functional Characterization of Endo- and Exo-Hydrolase Genes in Arabinan Degradation Gene Cluster of Bifidobacterium longum subsp. suis
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