Journal of Microbiology

Review

Adenoviral Vector System: A Comprehensive Overview of Constructions, Therapeutic Applications and Host Responses: Anyeseu Park, Jeong Yoon Lee; J. Microbiol. 2024;62(7):491-509. Published online July 22, 2024; DOI: https://doi.org/10.1007/s12275-024-00159-4

195 View
0 Download
7 Web of Science
7 Crossref

Adenoviral vectors are crucial for gene therapy and vaccine development, offering a platform for gene delivery into host cells. Since the discovery of adenoviruses, first-generation vectors with limited capacity have evolved to third-generation vectors flacking viral coding sequences, balancing safety and gene-carrying capacity. The applications of adenoviral vectors for gene therapy and anti-viral treatments have expanded through the use of in vitro ligation and homologous recombination, along with gene editing advancements such as CRISPR-Cas9. Current research aims to maintain the efficacy and safety of adenoviral vectors by addressing challenges such as pre-existing immunity against adenoviral vectors and developing new adenoviral vectors from rare adenovirus types and non-human species. In summary, adenoviral vectors have great potential in gene therapy and vaccine development. Through continuous research and technological advancements, these vectors are expected to lead to the development of safer and more effective treatments.

Citations

Citations to this article as recorded by

Engineering an oncolytic adenoviral platform for precise delivery of antisense peptide nucleic acid to modulate PD-L1 overexpression in cancer cells
Andrea Patrizia Falanga, Francesca Greco, Monica Terracciano, Stefano D’Errico, Maria Marzano, Sara Feola, Valentina Sepe, Flavia Fontana, Ilaria Piccialli, Vincenzo Cerullo, Hélder A. Santos, Nicola Borbone
International Journal of Pharmaceutics.2025; 668: 124941. CrossRef
Enhancing precision in cancer treatment: the role of gene therapy and immune modulation in oncology
Emile Youssef, Brandon Fletcher, Dannelle Palmer
Frontiers in Medicine.2025;[Epub] CrossRef
Protein-Based Degraders: From Chemical Biology Tools to Neo-Therapeutics
Lisha Ou, Mekedlawit T. Setegne, Jeandele Elliot, Fangfang Shen, Laura M. K. Dassama
Chemical Reviews.2025; 125(4): 2120. CrossRef
Intestinal mucus: the unsung hero in the battle against viral gastroenteritis
Waqar Saleem, Ateeqa Aslam, Mehlayl Tariq, Hans Nauwynck
Gut Pathogens.2025;[Epub] CrossRef
Chromatin structure and gene transcription of recombinant p53 adenovirus vector within host
Duo Ning, Yuqing Deng, Simon Zhongyuan Tian
Frontiers in Molecular Biosciences.2025;[Epub] CrossRef
Molecular Engineering of Virus Tropism
Bo He, Belinda Wilson, Shih-Heng Chen, Kedar Sharma, Erica Scappini, Molly Cook, Robert Petrovich, Negin P. Martin
International Journal of Molecular Sciences.2024; 25(20): 11094. CrossRef
Antisolvent 3D Printing of Gene-Activated Scaffolds for Bone Regeneration
Andrey Vyacheslavovich Vasilyev, Irina Alekseevna Nedorubova, Viktoria Olegovna Chernomyrdina, Anastasiia Yurevna Meglei, Viktoriia Pavlovna Basina, Anton Vladimirovich Mironov, Valeriya Sergeevna Kuznetsova, Victoria Alexandrovna Sinelnikova, Olga Anatol
International Journal of Molecular Sciences.2024; 25(24): 13300. CrossRef

Journal Article

Licochalcone A Protects Vaginal Epithelial Cells Against Candida albicans Infection Via the TLR4/NF-κB Signaling Pathway: Wei Li, Yujun Yin, Taoqiong Li, Yiqun Wang, Wenyin Shi; J. Microbiol. 2024;62(7):525-533. Published online May 31, 2024; DOI: https://doi.org/10.1007/s12275-024-00134-z

121 View
0 Download
1 Web of Science

Abstract: Vulvovaginal candidiasis (VVC) is a prevalent condition affecting a significant portion of women worldwide. Licochalcone A (LA), a natural compound with diverse biological activities, holds promise as a protective agent against Candida albicans (C. albicans) infection. This study aims to investigate the potential of LA to safeguard vaginal epithelial cells (VECs) from C. albicans infection and elucidate the underlying molecular mechanisms. To simulate VVC in vitro, VK2-E6E7 cells were infected with C. albicans. Candida albicans biofilm formation, C. albicans adhesion to VK2-E6E7 cells, and C. albicans-induced cell damage and inflammatory responses were assessed by XTT reduction assay, fluorescence assay, LDH assay, and ELISA. CCK-8 assay was performed to evaluate the cytotoxic effects of LA on VK2-E6E7 cells. Western blotting assay was performed to detect protein expression. LA dose-dependently hindered C. albicans biofilm formation and adhesion to VK2-E6E7 cells. Furthermore, LA mitigated cell damage, inhibited the Bax/Bcl-2 ratio, and attenuated the secretion of pro-inflammatory cytokines in C. albicans-induced VK2-E6E7 cells. The investigation into LA's impact on the Toll-like receptor 4 (TLR4)/nuclear factor-kappa B (NF-κB) pathway revealed that LA downregulated TLR4 expression and inhibited NF-κB activation in C. albicans-infected VK2-E6E7 cells. Furthermore, TLR4 overexpression partially abated LA-mediated protection, further highlighting the role of the TLR4/NF-κB pathway. LA holds the potential to safeguard VECs against C. albicans infection, potentially offering therapeutic avenues for VVC management.

Review

Balancing Act of the Intestinal Antimicrobial Proteins on Gut Microbiota and Health: Ye Eun Ra, Ye‑Ji Bang; J. Microbiol. 2024;62(3):167-179. Published online April 17, 2024; DOI: https://doi.org/10.1007/s12275-024-00122-3

73 View
1 Download
8 Web of Science
8 Crossref

Abstract

The human gut houses a diverse and dynamic microbiome critical for digestion, metabolism, and immune development, exerting profound efects on human health. However, these microorganisms pose a potential threat by breaching the gut barrier, entering host tissues, and triggering infections, uncontrolled infammation, and even sepsis. The intestinal epithelial cells form the primary defense, acting as a frontline barrier against microbial invasion. Antimicrobial proteins (AMPs), produced by these cells, serve as innate immune efectors that regulate the gut microbiome by directly killing or inhibiting microbes. Abnormal AMP production, whether insufcient or excessive, can disturb the microbiome equilibrium, contributing to various intestinal diseases. This review delves into the complex interactions between AMPs and the gut microbiota and sheds light on the role of AMPs in governing host-microbiota interactions. We discuss the function and mechanisms of action of AMPs, their regulation by the gut microbiota, microbial evasion strategies, and the consequences of AMP dysregulation in disease. Understanding these complex interactions between AMPs and the gut microbiota is crucial for developing strategies to enhance immune responses and combat infections within the gut microbiota. Ongoing research continues to uncover novel aspects of this intricate relationship, deepening our understanding of the factors shaping gut health. This knowledge has the potential to revolutionize therapeutic interventions, ofering enhanced treatments for a wide range of gut-related diseases.

Citations

Citations to this article as recorded by

Host-directed therapies modulating innate immunity against infection in hematologic malignancies
Qiong Wang, Kristján Hermannsson, Egill Másson, Peter Bergman, Guðmundur Hrafn Guðmundsson
Blood Reviews.2025; 70: 101255. CrossRef
Comparison of naturalization mouse model setups uncover distinct effects on intestinal mucosa depending on microbial experience
Henriette Arnesen, Signe Birkeland, Harriet Stendahl, Klaus Neuhaus, David Masopust, Preben Boysen, Harald Carlsen
Discovery Immunology.2025;[Epub] CrossRef
Oral administration of LEAP2 enhances immunity against Edwardsiella tarda through regulation of gut bacterial community and metabolite in mudskipper
Ting-Fang Zhu, Hai-Peng Guo, Li Nie, Jiong Chen
Fish & Shellfish Immunology.2025; 158: 110128. CrossRef
Pharmacology of Intestinal Inflammation and Repair
Céline Deraison, Nathalie Vergnolle
Annual Review of Pharmacology and Toxicology .2025; 65(1): 301. CrossRef
Macrophages and Gut Barrier Function: Guardians of Gastrointestinal Health in Post-Inflammatory and Post-Infection Responses
Edward Xiangtai Meng, George Nicholas Verne, Qiqi Zhou
International Journal of Molecular Sciences.2024; 25(17): 9422. CrossRef
Progress in the Identification and Design of Novel Antimicrobial Peptides Against Pathogenic Microorganisms
Shengwei Sun
Probiotics and Antimicrobial Proteins.2024;[Epub] CrossRef
Host-Associated Microbiome
Woo Jun Sul
Journal of Microbiology.2024; 62(3): 135. CrossRef
Gut Microbiota as Emerging Players in the Development of Alcohol-Related Liver Disease
Wei Li, Wenkang Gao, Shengqi Yan, Ling Yang, Qingjing Zhu, Huikuan Chu
Biomedicines.2024; 13(1): 74. CrossRef

Journal Articles

Yeast polyubiquitin unit regulates synaptonemal complex formation and recombination during meiosis: Min-Kyung Jo , Kiwon Rhee , Keun Pil Kim , Soogil Hong; J. Microbiol. 2022;60(7):705-714. Published online July 4, 2022; DOI: https://doi.org/10.1007/s12275-022-2204-y

57 View
0 Download
1 Web of Science
1 Crossref

Abstract

Ubiquitin is highly conserved in most eukaryotes and involved in diverse physiological processes, including cell division, protein quality control, and protein degradation mediated by the ubiquitin-proteasome system after heat shock, glucose-starvation, and oxidative stress. However, the role of the ubiquitin gene UBI4, which contains five consecutive head-to-tail ubiquitin repeats, in meiosis has not been investigated. In this study, we show that the Saccharomyces cerevisiae polyubiquitin precursor gene, UBI4, is required to promote synaptonemal complex (SC) formation and suppress excess doublestrand break formation. Moreover, the proportion of Zip1 polycomplexes, which indicate abnormal SC formation, in cells with a mutation in UBI4 (i.e., ubi4Δ cells) is higher than that of wild-type cells, implying that the UBI4 plays an important role in the early meiotic prophase I. Interestingly, although ubi4Δ cells rarely form full-length SCs in the pachytene stage of prophase I, the Zip3 foci are still seen, as in wild-type cells. Moreover, ubi4Δ cells proficiently form crossover and noncrossover products with a slight delay compared to wild-type cells, suggesting that UBI4 is dispensable in SCcoupled recombination. Our findings demonstrate that UBI4 exhibits dual functions that are associated with both positive and negative roles in SC formation and recombination during meiosis.

Citations

Citations to this article as recorded by

The deubiquitinase Usp7 in Drosophila melanogaster is required for synaptonemal complex maintenance
Cathleen M. Lake, Jennifer Gardner, Salam Briggs, Zulin Yu, Grace McKown, R. Scott Hawley
Proceedings of the National Academy of Sciences.2024;[Epub] CrossRef

Description of Vagococcus coleopterorum sp. nov., isolated from the intestine of the diving beetle, Cybister lewisianus, and Vagococcus hydrophili sp. nov., isolated from the intestine of the dark diving beetle, Hydrophilus acuminatus, and emended description of the genus Vagococcus: Dong-Wook Hyun , Euon Jung Tak , Pil Soo Kim , Jin-Woo Bae; J. Microbiol. 2021;59(2):132-141. Published online December 23, 2020; DOI: https://doi.org/10.1007/s12275-021-0485-1

47 View
0 Download
6 Web of Science
6 Crossref

Abstract

A polyphasic taxonomic approach was used to characterize two novel bacterial strains, HDW17AT and HDW17BT, isolated from the intestine of the diving beetle Cybister lewisianus, and the dark diving beetle Hydrophilus acuminatus, respectively. Both strains were Gram-positive and facultative anaerobic cocci forming cream-colored colonies. The isolates grew optimally at 25°C, pH 7, in the presence of 0.3% (wt/vol) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences and genome sequences showed that the isolates were members of the genus Vagococcus, and strain HDW17AT was closely related to Vagococcus fessus CCUG 41755T (98.9% of 16S rRNA gene sequence similarity and 74.3% of average nucleotide identity [ANI]), whereas strain HDW17BT was closely related to Vagococcus fluvialis NCFB 2497T (98.9% of 16S rRNA gene sequence similarity and 76.6% of ANI). Both strains contained C16:0, and C18:1 ω9c as the major cellular fatty acids, but C16:1 ω9c was also observed only in strain HDW17BT as the major cellular fatty acid. The respiratory quinone of the isolates was MK-7. The major polar lipid components were phosphatidylglycerol, phosphatidylethanolamine, and diphosphatidylglycerol. The genomic DNA G + C content of strains HDW17AT and HDW17BT were 36.6 and 34.4%, respectively. Both strains had cell wall peptidoglycan composed of the amino acids L-alanine, glycine, D-glutamic acid, L-tryptophan, L-lysine, and L-aspartic acid, and the sugars ribose, glucose, and galactose. Based on phylogenetic, phenotypic, chemotaxonomic, and genotypic analyses, strains HDW17AT and HDW17BT represent two novel species in the genus Vagococcus. We propose the name Vagococcus coleopterorum sp. nov. for strain HDW17AT (= KACC 21348T = KCTC 49324T = JCM 33674T) and the name Vagococcus hydrophili sp. nov. for strain HDW17BT (= KACC 21349T = KCTC 49325T = JCM 33675T).

Citations

Citations to this article as recorded by

Vagococcus proximus sp. nov. and Vagococcus intermedius sp. nov., originating from modified atmosphere packaged broiler meat
Per Johansson, Elina Jääskeläinen, Elina Säde, Johanna Björkroth
International Journal of Systematic and Evolutionary Microbiology .2023;[Epub] CrossRef
A case of Vagococcus fluvialis isolated from the bile of a patient with calculous cholecystitis
Dan Zhang, Xiaosu Wang, Jingdan Yu, Zheng Dai, Qichao Li, Litao Zhang
BMC Infectious Diseases.2023;[Epub] CrossRef
Vagococcus luciliae sp. nov., isolated from the common green bottle fly Lucilia sericata
Juan Guzman, Anja Poehlein, Rolf Daniel, Peter Kämpfer, Andreas Vilcinskas
International Journal of Systematic and Evolutionary Microbiology .2023;[Epub] CrossRef
Valid publication of new names and new combinations effectively published outside the IJSEM. Validation List no. 203
Aharon Oren, George M. Garrity
International Journal of Systematic and Evolutionary Microbiology .2022;[Epub] CrossRef
Effects of different doses of electron beam irradiation on bacterial community of Portunus trituberculatus
Huijuan Pan, Qi Yu, Chenru Qian, Haitao Shao, Jiajun Han, Yongyong Li, Yongjiang Lou
Food Bioscience.2021; 42: 101198. CrossRef
Description of Nocardioides piscis sp. nov., Sphingomonas piscis sp. nov. and Sphingomonas sinipercae sp. nov., isolated from the intestine of fish species Odontobutis interrupta (Korean spotted sleeper) and Siniperca scherzeri (leopard mandarin fish)
Dong-Wook Hyun, Yun-Seok Jeong, Jae-Yun Lee, Hojun Sung, So-Yeon Lee, Jee-Won Choi, Hyun Sik Kim, Pil Soo Kim, Jin-Woo Bae
Journal of Microbiology.2021; 59(6): 552. CrossRef

Autophagy of bovine mammary epithelial cell induced by intracellular Staphylococcus aureus: Na Geng , Kangping Liu , Jianwei Lu , Yuliang Xu , Xiaozhou Wang , Run Wang , Jianzhu Liu , Yongxia Liu , Bo Han; J. Microbiol. 2020;58(4):320-329. Published online February 26, 2020; DOI: https://doi.org/10.1007/s12275-020-9182-8

47 View
0 Download
19 Web of Science
18 Crossref

Abstract

Bovine mastitis is a common disease in the dairy industry that causes great economic losses. As the primary pathogen of contagious mastitis, Staphylococcus aureus (S. aureus) can invade bovine mammary epithelial cells, thus evading immune defenses and resulting in persistent infection. Recently, autophagy has been considered an important mechanism for host cells to clear intracellular pathogens. In the current study, autophagy caused by S. aureus was detected, and the correlation between autophagy and intracellular S. aureus survival was assessed. First, a model of intracellular S. aureus infection was established. Then, the autophagy of MAC-T cells was evaluated by confocal microscopy and western blot. Moreover, the activation of the PI3K-Akt-mTOR and ERK1/2 signaling pathways was determined by western blot. Finally, the relationship between intracellular bacteria and autophagy was analyzed by using autophagy regulators (3-methyladenine [3-MA], rapamycin [Rapa] and chloroquine [CQ]). The
results
showed that S. aureus caused obvious induction of autophagosome formation, transformation of LC3I/II, and degradation of p62/SQSTM1 in MAC-T cells; furthermore, the PI3K-Akt-mTOR and ERK1/2 signaling pathways were activated. The number of intracellular S. aureus increased significantly with autophagy activation by rapamycin, whereas the number decreased when the autophagy flux was inhibited by chloroquine. Therefore, this study indicated that intracellular S. aureus can induce autophagy and utilize it to survive in bovine mammary epithelial cells.

Citations

Citations to this article as recorded by

Carotenoids as modulators of the PI3K/Akt/mTOR pathway: innovative strategies in cancer therapy
Biswajit Kumar Utpal, Zerrouki Dehbia, B. M. Redwan Matin Zidan, Sherouk Hussein Sweilam, Laliteshwar Pratap Singh, M. S. Arunkumar, M. Sona, Uttam Prasad Panigrahy, R. Keerthana, Sandhya Rani Mandadi, Safia Obaidur Rab, Mohammed Ali Alshehri, Doukani Kou
Medical Oncology.2024;[Epub] CrossRef
Staphylococcus aureus regulates Th17 cells and autophagy via STING in chronic eosinophilic rhinosinusitis with nasal polyps
Weigang Gan, Xingchen Liu, Feng Liu, Junying Hu
European Archives of Oto-Rhino-Laryngology.2024;[Epub] CrossRef
Modulatory Effects of Regulated Cell Death: An Innovative Preventive Approach for the Control of Mastitis
Xiaojing Xia, Pengfei Ren, Yilin Bai, Jingjing Li, Huihui Zhang, Lei Wang, Jianhe Hu, Xinwei Li, Ke Ding
Cells.2024; 13(20): 1699. CrossRef
UID-Dual Transcriptome Sequencing Analysis of the Molecular Interactions between Streptococcus agalactiae ATCC 27956 and Mammary Epithelial Cells
Jishang Gong, Taotao Li, Yuanfei Li, Xinwei Xiong, Jiguo Xu, Xuewen Chai, Youji Ma
Animals.2024; 14(17): 2587. CrossRef
Analysis of differentially expressed microRNAs in bovine mammary epithelial cells treated with lipoteichoic acid
Puxiu Shen, Jingcheng Yu, Chenbo Yan, Dexin Yang, Chao Tong, Xinzhuang Wang
Journal of Animal Physiology and Animal Nutrition.2023; 107(2): 463. CrossRef
PINK1/Parkin‐mediated mitophagy enhances the survival of Staphylococcus aureus in bovine macrophages
Xi Zhou, Kangjun Liu, Jianji Li, Luying Cui, Junsheng Dong, Jun Li, Xia Meng, Guoqiang Zhu, Heng Wang
Journal of Cellular and Molecular Medicine.2023; 27(3): 412. CrossRef
Chlorogenic acid enhances PPARγ-mediated lipogenesis through preventing Lipin 1 nuclear translocation in Staphylococcus aureus-exposed bovine mammary epithelial cells
Ruiyuan Yao, Manshulin Wang, Yue Zhao, Qiang Ji, Xue Feng, Linfeng Bai, Lili Bao, Yanfeng Wang, Huifang Hao, Xihe Li, Zhigang Wang
Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids.2023; 1868(11): 159396. CrossRef
Staphylococcus aureus induces mitophagy to promote its survival within bovine mammary epithelial cells
Dianwen Xu, Guiqiu Hu, Jianchun Luo, Ji Cheng, Di Wu, Lisha Cheng, Xuejie Huang, Shoupeng Fu, Juxiong Liu
Veterinary Microbiology.2023; 280: 109697. CrossRef
Subacute ruminal acidosis downregulates FOXA2, changes oxidative status, and induces autophagy in the livers of dairy cows fed a high-concentrate diet
Hongzhu Zhang, Yang Xue, Wan Xie, Yan Wang, Nana Ma, Guangjun Chang, Xiangzhen Shen
Journal of Dairy Science.2023; 106(3): 2007. CrossRef
Activation of PINK1-mediated mitophagy protects bovine mammary epithelial cells against lipopolysaccharide-induced mitochondrial and inflammatory damage in vitro
Renxu Chang, Yan Tang, Hongdou Jia, Zhihao Dong, Shuang Gao, Qian Song, Hao Dong, Qiushi Xu, Qianming Jiang, Juan J. Loor, Xudong Sun, Chuang Xu
Free Radical Biology and Medicine.2023; 194: 172. CrossRef
Incomplete autophagy promotes the proliferation of Mycoplasma hyopneumoniae through the JNK and Akt pathways in porcine alveolar macrophages
Yukang Wen, Zhengkun Chen, Yaqin Tian, Mei Yang, Qingshuang Dong, Yujiao Yang, Honglei Ding
Veterinary Research.2022;[Epub] CrossRef
Academy of Breastfeeding Medicine Clinical Protocol #36: The Mastitis Spectrum, Revised 2022
Katrina B. Mitchell, Helen M. Johnson, Juan Miguel Rodríguez, Anne Eglash, Charlotte Scherzinger, Kyle Widmer, Pamela Berens, Brooke Miller
Breastfeeding Medicine.2022; 17(5): 360. CrossRef
Streptococcus agalactiae-induced autophagy of bovine mammary epithelial cell via PI3K/AKT/mTOR pathway
Mengzhu Qi, Hao Geng, Na Geng, Yukun Cui, Changxi Qi, Guodong Cheng, Kaimin Song, Liping Hu, Yongxia Liu, Jianzhu Liu, Bo Han
Journal of Dairy Research.2022; 89(2): 178. CrossRef
High-dose lipopolysaccharide induced autophagic cell death in bovine mammary alveolar cells
Jin-Ki Park, Joon Mo Yeo, Kwanghyun Cho, Hyun-Jung Park, Won-Young Lee
Journal of Animal Reproduction and Biotechnology.2022; 37(3): 169. CrossRef
Selenium Alleviates Inflammation in Staphylococcus aureus-Induced Mastitis via MerTK-Dependent Activation of the PI3K/Akt/mTOR Pathway in Mice
Si-jie Chen, Chen-yuan Zhang, Di Yu, Chang-jie Lin, Hao-jun Xu, Chang-min Hu
Biological Trace Element Research.2022; 200(4): 1750. CrossRef
Potential role of β‑carotene‑modulated autophagy in puerperal breast inflammation (Review)
Stella Hasianna, Julia Gunadi, Enny Rohmawaty, Ronny Lesmana
Biomedical Reports.2022;[Epub] CrossRef
Intracellular Staphylococcus aureus inhibits autophagy of bovine mammary epithelial cells through activating p38α
Run Wang, Wen Zhang, Lumei Wang, Na Geng, Xiaozhou Wang, Meihua Zhang, Jianzhu Liu, Yongxia Liu, Bo Han
Journal of Dairy Research.2021; 88(3): 293. CrossRef
Autophagy in Staphylococcus aureus Infection
Mengyao Wang, Ziyao Fan, Hongbing Han
Frontiers in Cellular and Infection Microbiology.2021;[Epub] CrossRef

Pten gene deletion in intestinal epithelial cells enhances susceptibility to Salmonella Typhimurium infection in mice: Cody Howe , Jonathon Mitchell , Su Jin Kim , Eunok Im , Sang Hoon Rhee; J. Microbiol. 2019;57(11):1012-1018. Published online September 25, 2019; DOI: https://doi.org/10.1007/s12275-019-9320-3

55 View
0 Download
9 Web of Science
9 Crossref

Abstract

Although phosphatase and tensin homolog (PTEN) is typically considered a tumor-suppressor gene, it was recently suggested that PTEN regulates TLR5-induced immune and inflammatory responses in intestinal epithelial cells (IECs), suggesting an immunomodulatory function of PTEN in the gut. However, this alternative function of PTEN has not yet been evaluated in an in vivo context of protection against enteropathogenic bacteria. To address this, we utilized IECrestricted Pten knockout (PtenΔIEC/ΔIEC) and littermate Pten+/+ mice. These mice were subjected to the streptomycin-pretreated mouse model of Salmonella infection, and subsequently given an oral gavage of a low inoculum (2 × 104 CFU) of Salmonella enterica serovar Typhimurium (S. Typhimurium). This bacterial infection not only increased the mortality of PtenΔIEC/ΔIEC mice compared to Pten+/+ mice, but also induced deleterious gastrointestinal inflammation in PtenΔIEC/ΔIEC mice manifested by massive histological damage to the intestinal mucosa. S. Typhimurium infection upregulated pro-inflammatory cytokine production in the intestine of PtenΔIEC/ΔIEC mice compared to controls. Furthermore, bacterial loads were greatly increased in the liver, mesenteric lymph node, and spleen of PtenΔIEC/ΔIEC mice compared to controls. Together, these results suggest that IEC-restricted Pten deficiency renders the host greatly susceptible to Salmonella infection and support an immuneregulatory role of PTEN in the gut.

Citations

Citations to this article as recorded by

Exploring Protein Functions of Gut Bacteriome and Mycobiome in Thai Infants Associated with Atopic Dermatitis Through Metaproteomic and Host Interaction Analysis
Thanawit Chantanaskul, Preecha Patumcharoenpol, Sittirak Roytrakul, Amornthep Kingkaw, Wanwipa Vongsangnak
International Journal of Molecular Sciences.2024; 25(24): 13533. CrossRef
QTL Mapping-Based Identification of Visceral White-Nodules Disease Resistance Genes in Larimichthys polyactis
Qian Li, Jiajie Zhu, Sifang Liu, Haowen Liu, Tianle Zhang, Ting Ye, Bao Lou, Feng Liu
International Journal of Molecular Sciences.2024; 25(20): 10872. CrossRef
Cannabinoid enhancement of lncRNA MMP25-AS1/MMP25 interaction reduces neutrophil infiltration and intestinal epithelial injury in HIV/SIV infection
Lakmini S. Premadasa, Eunhee Lee, Marina McDew-White, Xavier Alvarez, Sahana Jayakumar, Binhua Ling, Chioma M. Okeoma, Siddappa N. Byrareddy, Smita Kulkarni, Mahesh Mohan
JCI Insight.2023;[Epub] CrossRef
Regulator of RNase E activity modulates the pathogenicity of Salmonella Typhimurium
Jaejin Lee, Eunkyoung Shin, Ji-Hyun Yeom, Jaeyoung Park, Sunwoo Kim, Minho Lee, Kangseok Lee
Microbial Pathogenesis.2022; 165: 105460. CrossRef
Endoribonuclease-mediated control of hns mRNA stability constitutes a key regulatory pathway for Salmonella Typhimurium pathogenicity island 1 expression
Minho Lee, Minkyung Ryu, Minju Joo, Young-Jin Seo, Jaejin Lee, Hong-Man Kim, Eunkyoung Shin, Ji-Hyun Yeom, Yong-Hak Kim, Jeehyeon Bae, Kangseok Lee, William Navarre
PLOS Pathogens.2021; 17(2): e1009263. CrossRef
Corticotropin-Releasing Hormone Receptor Alters the Tumor Development and Growth in Apcmin/+ Mice and in a Chemically-Induced Model of Colon Cancer
Yunna Lee, Elise L. Ma, Marisa Patel, Gayoung Kim, Cody Howe, Charalabos Pothoulakis, Yong Sung Kim, Eunok Im, Sang Hoon Rhee
International Journal of Molecular Sciences.2021; 22(3): 1043. CrossRef
Phosphatase and Tensin Homolog in Non-neoplastic Digestive Disease: More Than Just Tumor Suppressor
Tianyu He, Xiaoyun Zhang, Jianyu Hao, Shigang Ding
Frontiers in Physiology.2021;[Epub] CrossRef
Phosphatase and Tensin Homolog (PTEN) of Japanese Flounder—Its Regulation by miRNA and Role in Autophagy, Apoptosis and Pathogen Infection
Wenrui Li, Xiaolu Guan, Li Sun
International Journal of Molecular Sciences.2020; 21(20): 7725. CrossRef
Chlorogenic Acid Promotes Autophagy and Alleviates Salmonella Typhimurium Infection Through the lncRNAGAS5/miR-23a/PTEN Axis and the p38 MAPK Pathway
Shirui Tan, Fang Yan, Qingrong Li, Yaping Liang, Junxu Yu, Zhenjun Li, Feifei He, Rongpeng Li, Ming Li
Frontiers in Cell and Developmental Biology.2020;[Epub] CrossRef

Research Support, Non-U.S. Gov't

Involvement of Phosphatidylinositol 3-Kinase/Akt Signaling Pathway in β1 Integrin-Mediated Internalization of Staphylococcus aureus by Alveolar Epithelial Cells: Jia-He Wang , Ke Zhang , Nan Wang , Xiao-Min Qiu , Yi-Bing Wang , Ping He; J. Microbiol. 2013;51(5):644-650. Published online June 25, 2013; DOI: https://doi.org/10.1007/s12275-013-3040-x

51 View
0 Download
19 Crossref

Abstract

The invasion of Staphylococcus aureus into alveolar epithelial cells is regarded as the key step for S. aureus lung infection. However, the mechanism of internalization of S. aureus by alveolar epithelial cells is not clear, and was the aim of this investigation Human lung adenocarcinomic epithelial cells and A549 cells were used. Human β1 integrin and rat β1 integrin were detected by real-time reverse transcription (RT)-PCR. The expressions of β1 integrin, Akt and p-Akt were detected by Western blot analysis. To further investigate the role of β1 integrin in S. aureus internalization by alveolar epithelial cells, we next performed siRNA-mediated knockdown of β1 integrin expression. In this study, we found that S. aureus invades human alveolar epithelial cells and rat primary alveolar epithelial cells. The β1 integrin ligand competitive inhibitor, GRGDS-peptide, blocked the internalization of S. aureus by A549 cells. Knockdown of β1 integrin also inhibited the internalization of S. aureus. In addition, the PI3K/Akt signaling pathway in alveolar epithelial cells was activated by the infection with S. aureus. Furthermore, Akt phosphorylation was abolished by transient transfection with β1 integrin siRNA in A549 cells challenged with S. aureus. Our results suggest that the phosphatidylinositol 3-kinase/Akt signaling pathway plays an important role in β1 integrin-mediated internalization of S. aureus by alveolar epithelial cells.

Citations

Citations to this article as recorded by

Effects of Titanium Dioxide Nanoparticles on Cell Growth and Migration of A549 Cells under Simulated Microgravity
Mei Wang, Jinxia Li, Shunyu Zhang, Yue You, Xianyu Zhu, Huandong Xiang, Liang Yan, Feng Zhao, Yunhui Li
Nanomaterials.2022; 12(11): 1879. CrossRef
Host Response of Human Epidermis to Methicillin-Resistant Staphylococcus aureus Biofilm Infection and Synthetic Antibiofilm Peptide Treatment
Bing (Catherine) Wu, Travis M. Blimkie, Evan F. Haney, Reza Falsafi, Noushin Akhoundsadegh, Robert E. W. Hancock
Cells.2022; 11(21): 3459. CrossRef
Non-Canonical Host Intracellular Niche Links to New Antimicrobial Resistance Mechanism
Michaela Kember, Shannen Grandy, Renee Raudonis, Zhenyu Cheng
Pathogens.2022; 11(2): 220. CrossRef
Identification and Characterization of Staphylococcus delphini Internalization Pathway in Nonprofessional Phagocytic Cells
Yousef Maali, Alan Diot, Patrícia Martins-Simões, Michele Bes, Daniel Bouvard, François Vandenesch, Paul O. Verhoeven, Frederic Laurent, Sophie Trouillet-Assant, Victor J. Torres
Infection and Immunity.2020;[Epub] CrossRef
Study on Intervention Mechanism of Yiqi Huayu Jiedu Decoction on ARDS Based on Network Pharmacology
Xu Liang, Changyong Luo, Yan Li, Xin Li, Qian Wang, Shujing Zhang, Qingqiao Sun, Yuanhong Ma, Caihua Xiong, Yanpeng Zeng, Huu-Tung Nguyen
Evidence-Based Complementary and Alternative Medicine.2020;[Epub] CrossRef
Characterization of the Atl-mediated staphylococcal internalization mechanism
Tim Schlesier, Anke Siegmund, Ursula Rescher, Christine Heilmann
International Journal of Medical Microbiology.2020; 310(8): 151463. CrossRef
Microbial uptake by the respiratory epithelium: outcomes for host and pathogen
Margherita Bertuzzi, Gemma E Hayes, Elaine M Bignell
FEMS Microbiology Reviews.2019; 43(2): 145. CrossRef
Human Bronchial Epithelial Cells Inhibit Aspergillus fumigatus Germination of Extracellular Conidia via FleA Recognition
Nicolas Richard, Léa Marti, Annabelle Varrot, Loïc Guillot, Juliette Guitard, Christophe Hennequin, Anne Imberty, Harriet Corvol, Michel Chignard, Viviane Balloy
Scientific Reports.2018;[Epub] CrossRef
Staphylococcal Adhesion and Host Cell Invasion: Fibronectin-Binding and Other Mechanisms
Jérôme Josse, Frédéric Laurent, Alan Diot
Frontiers in Microbiology.2017;[Epub] CrossRef
α‐Tocopheryl Phosphate Induces VEGF Expression via CD36/PI3Kγ in THP‐1 Monocytes
Jean‐Marc Zingg, Angelo Azzi, Mohsen Meydani
Journal of Cellular Biochemistry.2017; 118(7): 1855. CrossRef
Increased survival and proliferation of the epidemic strain Mycobacterium abscessus subsp. massiliense CRM0019 in alveolar epithelial cells
Giovanni Monteiro Ribeiro, Cristianne Kayoko Matsumoto, Fernando Real, Daniela Teixeira, Rafael Silva Duarte, Renato Arruda Mortara, Sylvia Cardoso Leão, Cristiane de Souza Carvalho-Wodarz
BMC Microbiology.2017;[Epub] CrossRef
Orthopedic implant infections: Incompetence of Staphylococcus epidermidis, Staphylococcus lugdunensis, and Enterococcus faecalis to invade osteoblasts
Davide Campoccia, Francesca Testoni, Stefano Ravaioli, Ilaria Cangini, Alessandra Maso, Pietro Speziale, Lucio Montanaro, Livia Visai, Carla Renata Arciola
Journal of Biomedical Materials Research Part A.2016; 104(3): 788. CrossRef
Association of intracellular Staphylococcus aureus with prognosis in chronic rhinosinusitis
Judy Ou, Amanda Drilling, Deepti Singhal, Neil C.‐W. Tan, Deanna Wallis‐Hill, Sarah Vreugde, Alkis J. Psaltis, Peter‐John Wormald
International Forum of Allergy & Rhinology.2016; 6(8): 792. CrossRef
Interferon‐γ enhances both the anti‐bacterial and the pro‐inflammatory response of human mast cells to Staphylococcus aureus
Emily J. Swindle, Jared M. Brown, Madeleine Rådinger, Frank R. DeLeo, Dean D. Metcalfe
Immunology.2015; 146(3): 470. CrossRef
Attenuation of the Phosphatidylinositol 3-Kinase/Akt Signaling Pathway by Porphyromonas gingivalis Gingipains RgpA, RgpB, and Kgp
Masaaki Nakayama, Tetsuyoshi Inoue, Mariko Naito, Koji Nakayama, Naoya Ohara
Journal of Biological Chemistry.2015; 290(8): 5190. CrossRef
Transcriptional Profile of Mycobacterium tuberculosis Replicating in Type II Alveolar Epithelial Cells
Michelle B. Ryndak, Krishna K. Singh, Zhengyu Peng, Suman Laal, Ludovic Tailleux
PLOS ONE.2015; 10(4): e0123745. CrossRef
The herbal-derived honokiol and magnolol enhances immune response to infection with methicillin-sensitive Staphylococcus aureus (MSSA) and methicillin-resistant S. aureus (MRSA)
Eun-Jin Choi, Hyung-Ip Kim, Ji-Ae Kim, Soo Youn Jun, Sang Hyeon Kang, Dong June Park, Seok-Jun Son, Younghoon Kim, Ok Sarah Shin
Applied Microbiology and Biotechnology.2015; 99(10): 4387. CrossRef
Nonprofessional Phagocytic Cell Receptors Involved inStaphylococcus aureusInternalization
Nayeli Alva-Murillo, Joel Edmundo López-Meza, Alejandra Ochoa-Zarzosa
BioMed Research International.2014; 2014: 1. CrossRef
Comparative proteome analysis reveals conserved and specific adaptation patterns of Staphylococcus aureus after internalization by different types of human non-professional phagocytic host cells
Kristin Surmann, Stephan Michalik, Petra Hildebrandt, Philipp Gierok, Maren Depke, Lars Brinkmann, JÃ¶rg Bernhardt, Manuela G. Salazar, Zhi Sun, David Shteynberg, Ulrike Kusebauch, Robert L. Moritz, Bernd Wollscheid, Michael Lalk, Uwe VÃ¶lker, Frank Schmi
Frontiers in Microbiology.2014;[Epub] CrossRef

First
Prev
Page of 1
Next
Last

Search