Research Support, Non-U.S. Gov't
- Effects of Fengycin from Bacillus subtilis fmbJ on Apoptosis and Necrosis in Rhizopus stolonifer
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Qunyong Tang , Xiaomei Bie , Zhaoxin Lu , Fengxia Lv , Yang Tao , Xiaoxu Qu
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J. Microbiol. 2014;52(8):675-680. Published online August 1, 2014
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DOI: https://doi.org/10.1007/s12275-014-3605-3
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Abstract
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The lipopeptide antibiotic fengycin, produced by Bacillus subtilis, strongly inhibits growth of filamentous fungi. In this study, we evaluated the effects of fengycin treatment on apoptosis and necrosis in Rhizopus stolonifer by means of cell staining and epifluorescence microscopy. At fengycin concentrations less than 50 μg/ml, treated fungal cells demonstrated a dose-dependent increase in apoptosis-associated markers compared with the untreated control. These markers included chromatin condensation, reactive oxygen species accumulation, mitochondrial membrane potential depolarization, phosphatidylserine externalization, and the occurrence of DNA strand breaks. These results showed that fungal cells were impaired in a number of important functions and entered apoptosis upon treatment with low concentrations of fengycin. In contrast, high concentrations (>50 μg/ml) induced necrosis, indicating that the fungicidal action of fengycin operates via two modes: apoptosis at low concentrations and necrosis at high concentrations. Additionally, the apoptotic effect that we have shown suggests that lower concentrations of fengycin than previously thought may be effective for food preservation.
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Journal Article
- Cyclic Lipopeptide Profile of Three Bacillus subtilis Strains; Antagonists of Fusarium Head Blight
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Christopher A. Dunlap , David A. Schisler , Neil P. Price , Steven F. Vaughn
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J. Microbiol. 2011;49(4):603-609. Published online September 2, 2011
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DOI: https://doi.org/10.1007/s12275-011-1044-y
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Abstract
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The objective of the study was to identify the lipopetides associated with three Bacillus subtilis strains.
The strains are antagonists of Gibberella zeae, and have been shown to be effective in reducing Fusarium
head blight in wheat. The lipopeptide profile of three B. subtilis strains (AS43.3, AS43.4, and OH131.1)
was determined using mass spectroscopy. Strains AS43.3 and AS43.4 produced the anti-fungal lipopeptides
from the iturin and fengycin family during the stationary growth phase. All three strains produced the
lipopeptide surfactin at different growth times. Strain OH131.1 only produced surfactin under these conditions.
The antifungal activity of the culture supernatant and individual lipopeptides was determined by the inhibition
of G. zeae. Cell-free supernatant from strains AS43.3 and AS43.4 demonstrated strong antibiosis of G. zeae,
while strain OH131.1 had no antibiosis activity. These results suggest a different mechanism of antagonism
for strain OH131.1, relative to AS43.3 and AS43.4.
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Research Support, Non-U.S. Gov't
- Antifungal Activity and Mechanism of Fengycin in the Presence and Absence of Commercial Surfactin Against Rhizopus stolonifer
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Yang Tao , Xiao-mei Bie , Feng-xia Lv , Hai-zhen Zhao , Zhao-xin Lu
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J. Microbiol. 2011;49(1):146-150. Published online March 3, 2011
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DOI: https://doi.org/10.1007/s12275-011-0171-9
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Abstract
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The antifungal activity and mechanism of fengycin in the presence and absence of commercial surfactin against Rhizopus stolonifer were investigated. The MIC (minimal inhibitory concentration) of fengycin without commercial surfactin added was 0.4 mg/ml while the MIC of fengycin with commercial surfactin added was 2.0 mg/ml. Fengycin acted on cell membrane and cellular organs and inhibited DNA synthesis. The antifungal effect of fengycin was reduced after commercial surfactin was added. All these results suggest that the fungal cell membrane may be the primary target of fengycin action and commercial surfactin may reduce the antifungal activity of fengycin.