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- Genetic linkage map construction and quantitative trait loci mapping of agronomic traits in Gloeostereum incarnatum
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Wan-Zhu Jiang , Fang-Jie Yao , Li-Xin Lu , Ming Fang , Peng Wang , You-Min Zhang , Jing-Jing Meng , Jia Lu , Xiao-Xu Ma , Qi He , Kai-Sheng Shao
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J. Microbiol. 2021;59(1):41-50. Published online November 17, 2020
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DOI: https://doi.org/10.1007/s12275-021-0242-5
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Abstract
- Gloeostereum incarnatum is an edible medicinal mushroom
widely grown in China. Using the whole genome of G. incarnatum,
simple sequence repeat (SSR) markers were developed
and synthetic primers were designed to construct its
first genetic linkage map. The 1,048.6 cm map is composed of
10 linkage groups and contains 183 SSR markers. In total,
112 genome assembly sequences were anchored, representing
16.43 Mb and covering 46.41% of the genome. Selfing
populations were used for quantitative trait loci (QTL) targeting,
and the composite interval mapping method was used
to co-localize the mycelium growth rate (potato dextrose agar
and sawdust), growth period, yield and fruiting body length,
and width and thickness. The 14 QTLs of agronomic traits
had LOD values of 3.20–6.51 and contribution rates of 2.22–
13.18%. No linkage relationship was found between the mycelium
growth rate and the growth period, but a linkage relationship
was observed among the length, width and thickness
of the fruiting bodies. Using NCBI’s BLAST alignment,
the genomic sequences corresponding to the QTL regions
were compared, and a TPR-like protein candidate gene was
selected. Using whole-genome data, 138 candidate genes were
found in four sequence fragments of two SSR markers located
in the same scaffold. The genetic map and QTLs established
in this study will aid in developing selective markers
for agronomic traits and identifying corresponding genes,
thereby providing a scientific basis for the further gene mapping
of quantitative traits and the marker-assisted selection
of functional genes in G. incarnatum breeding programs.
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