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- Genetic linkage map construction and quantitative trait loci mapping of agronomic traits in Gloeostereum incarnatum
- Wan-Zhu Jiang , Fang-Jie Yao , Li-Xin Lu , Ming Fang , Peng Wang , You-Min Zhang , Jing-Jing Meng , Jia Lu , Xiao-Xu Ma , Qi He , Kai-Sheng Shao
- J. Microbiol. 2021;59(1):41-50. Published online November 17, 2020
- DOI: https://doi.org/10.1007/s12275-021-0242-5
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- 7 Web of Science
- 6 Crossref
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Abstract
- Gloeostereum incarnatum is an edible medicinal mushroom widely grown in China. Using the whole genome of G. incarnatum, simple sequence repeat (SSR) markers were developed and synthetic primers were designed to construct its first genetic linkage map. The 1,048.6 cm map is composed of 10 linkage groups and contains 183 SSR markers. In total, 112 genome assembly sequences were anchored, representing 16.43 Mb and covering 46.41% of the genome. Selfing populations were used for quantitative trait loci (QTL) targeting, and the composite interval mapping method was used to co-localize the mycelium growth rate (potato dextrose agar and sawdust), growth period, yield and fruiting body length, and width and thickness. The 14 QTLs of agronomic traits had LOD values of 3.20–6.51 and contribution rates of 2.22– 13.18%. No linkage relationship was found between the mycelium growth rate and the growth period, but a linkage relationship was observed among the length, width and thickness of the fruiting bodies. Using NCBI’s BLAST alignment, the genomic sequences corresponding to the QTL regions were compared, and a TPR-like protein candidate gene was selected. Using whole-genome data, 138 candidate genes were found in four sequence fragments of two SSR markers located in the same scaffold. The genetic map and QTLs established in this study will aid in developing selective markers for agronomic traits and identifying corresponding genes, thereby providing a scientific basis for the further gene mapping of quantitative traits and the marker-assisted selection of functional genes in G. incarnatum breeding programs.
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Citations
Citations to this article as recorded by
- Dicarboxylic Amino Acid Permease 7219 Regulates Fruiting Body Type of Auricularia heimuer
Jia Lu, Lixin Lu, Fangjie Yao, Ming Fang, Xiaoxu Ma, Jingjing Meng, Kaisheng Shao
Journal of Fungi.2023; 9(9): 876. CrossRef - Identification of quantitative trait loci for growth traits in red swamp crayfish (Procambarus clarkii)
Junxiao Sun, Cuirong Luo, Bo Peng, Guohui Peng, Yunfei Tan, Xufeng Bai
Aquaculture and Fisheries.2023; 8(6): 727. CrossRef - Improved Genetic Map and Localization of Quantitative Trait Loci for Quality Traits in Auricularia heimuer
Jia Lu, Ming Fang, Fangjie Yao, Lixin Lu, Xiaoxu Ma, Jingjing Meng, Kaisheng Shao
Horticulturae.2023; 9(7): 763. CrossRef - Assemblies of the genomes of parasitic wasps using meta-assembly and scaffolding with genetic linkage
Kameron T Wittmeyer, Sara J Oppenheim, Keith R Hopper, J Hesselberth
G3 Genes|Genomes|Genetics.2022;[Epub] CrossRef - EST-SSR Primer Development and Genetic Structure Analysis of Psathyrostachys juncea Nevski
Zhen Li, Lan Yun, Zhiqi Gao, Tian Wang, Xiaomin Ren, Yan Zhao
Frontiers in Plant Science.2022;[Epub] CrossRef - Analysis of the Genome Sequence of Strain GiC-126 of Gloeostereum incarnatum with Genetic Linkage Map
Wan-Zhu Jiang, Fang-Jie Yao, Ming Fang, Li-Xin Lu, You-Min Zhang, Peng Wang, Jing-Jing Meng, Jia Lu, Xiao-Xu Ma, Qi He, Kai-Sheng Shao, Asif Ali Khan, Yun-Hui Wei
Mycobiology.2021; 49(4): 406. CrossRef
- Dicarboxylic Amino Acid Permease 7219 Regulates Fruiting Body Type of Auricularia heimuer
Research Support, Non-U.S. Gov't
- Proteomic Analysis of Hyphae-Specific Proteins That Are Expressed Differentially in cakem1/cakem1 Mutant Strains of Candida albicans
- Kang-Hoon Lee , Seung-Yeop Kim , Jong-Hwan Jung , Jinmi Kim
- J. Microbiol. 2010;48(3):365-371. Published online June 23, 2010
- DOI: https://doi.org/10.1007/s12275-010-9155-4
- 38 View
- 0 Download
- 8 Scopus
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Abstract
- The yeast-to-hyphal transition is a major virulence factor in the fungal pathogen Candida albicans. Mutations in the CaKEM1 gene, which encodes a 5′-3′ exoribonuclease responsible for mRNA degradation, show a defect in hyphal growth. We applied two-dimensional gel electrophoresis to identify hyphae-specific proteins that have altered expressions in the presence of the cakem1 mutation. Eight proteins, Eno1, Eps1, Fba1, Imh3, Lpd1, Met6, Pdc11, and Tsa1 were upregulated during hyphal transition in wild-type but not in cakem1/cakem1 mutant cells. A second group of proteins, Idh1, Idh2, and Ssb1, showed increased levels of expression in cakem1/cakem1 mutant cells when compared to wild-type cells. Overexpression of Lpd1, a component of the pyruvate dehydrogenase complex, caused slight hyperfilamentation in a wild-type strain and suppressed the filamentation defect of the cakem1 mutation. The Ssb1 protein, which is a potential heat shock protein, and the Imh3 protein, which is a putative enzyme in GMP biosynthesis also showed the filamentation-associated phenotypes.
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