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- Minireview] Electron microscopic observations of prokaryotic surface appendages
- Ki Woo Kim
- J. Microbiol. 2017;55(12):919-926. Published online December 7, 2017
- DOI: https://doi.org/10.1007/s12275-017-7369-4
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- 6 Crossref
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Abstract
- Prokaryotic microbes possess a variety of appendages on their cell surfaces. The most commonly known surface appendages of bacteria include flagella, pili, curli, and spinae. Although archaea have archaella (archaeal flagella) and various types of pili that resemble those in bacteria, cannulae, and hami are unique to archaea. Typically involved in cell motility, flagella, the thickest appendages, are 20–26 nm and 10–14 nm wide in bacteria and archaea, respectively. Bacterial and archaeal pili are distinguished by their thin, short, hair-like structures. Curli appear as coiled and aggregative thin fibers, whereas spinae are tubular structures 50–70 nm in diameter in bacteria. Cannulae are characterized by ~25 nm-wide tubules that enter periplasmic spaces and connect neighboring archaeal cells. Hami are 1–3 μm in length and similar to barbed grappling hooks for attachment to bacteria. Recent advances in specimen preparation methods and image processing techniques have made cryo-transmission electron microscopy an essential tool for in situ structural analysis of microbes and their extracellular structures.
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- Mechanism of escape from the antibacterial activity of metal-based nanoparticles in clinically relevant bacteria: A systematic review
Marco Felipe Salas-Orozco, Ana Cecilia Lorenzo-Leal, Idania de Alba Montero, Nuria Patiño Marín, Miguel Angel Casillas Santana, Horacio Bach
Nanomedicine: Nanotechnology, Biology and Medicine.2024; 55: 102715. CrossRef - Symbiotic Interactions of Archaea in Animal and Human Microbiomes
Nancy Weiland-Bräuer
Current Clinical Microbiology Reports.2023; 10(4): 161. CrossRef - Jejubacter calystegiae gen. nov., sp. nov., moderately halophilic, a new member of the family Enterobacteriaceae, isolated from beach morning glory
Lingmin Jiang, Dexin Wang, Jung-Sook Lee, Dae-Hyuk Kim, Jae Cheol Jeong, Cha Young Kim, Suk Weon Kim, Jiyoung Lee
Journal of Microbiology.2020; 58(5): 357. CrossRef - Role of Bacterial Cytoskeleton and Other Apparatuses in Cell Communication
Divya Singhi, Preeti Srivastava
Frontiers in Molecular Biosciences.2020;[Epub] CrossRef - A versatile remote control system for functional expression of bacterial virulence genes based on the tetA promoter
Marc Schulte, Torsten Sterzenbach, Katarzyna Miskiewicz, Laura Elpers, Michael Hensel, Nicole Hansmeier
International Journal of Medical Microbiology.2019; 309(1): 54. CrossRef - Paracoccus jeotgali sp. nov., isolated from Korean salted and fermented shrimp
Juseok Kim, Joon Yong Kim, Hye Seon Song, In-Tae Cha, Seong Woon Roh, Se Hee Lee
Journal of Microbiology.2019; 57(6): 444. CrossRef
- Mechanism of escape from the antibacterial activity of metal-based nanoparticles in clinically relevant bacteria: A systematic review
Research Support, Non-U.S. Gov't
- Isolation, Characterization, and Abundance of Filamentous Members of Caldilineae in Activated Sludge
- Dae-No Yoon , Soo-Je Park , So-Jeong Kim , Che Ok Jeon , Jong-Chan Chae , Sung-Keun Rhee
- J. Microbiol. 2010;48(3):275-283. Published online June 23, 2010
- DOI: https://doi.org/10.1007/s12275-010-9366-8
- 43 View
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- 49 Scopus
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Abstract
- Chloroflexi are currently believed to serve as backbone forming agents in the activated sludge of wastewater treatment plants (WWTPs). In this study, we isolated and characterized filamentous bacteria in the class Caldilineae of the phylum Chloroflexi in municipal WWTPs. Diversity analysis using Chloroflexi-specific 16S rRNA gene clone libraries showed that 97% of the clones belonged to the subdivision Anaerolineae comprising the two classes Anaerolineae (95%) and Caldilineae (2%). Clones of Caldilineae were related to a thermophilic filament Caldilinea aerophila with 93% 16S rRNA gene sequence similarity. We obtained filamentous isolates classified into the class Caldilineae showing the best match to C. aerophila with 89% 16S rRNA gene sequence similarity. Isolates showed no ability to assimilate glucose or N-acetylglucosamine or to degrade biopolymers which were observed in filamentous Chloroflexi of WWTPs. The assessment of relative abundance based on quantitative PCR of the 16S rRNA gene indicated that members of the class Caldilineae comprised 12-19% of the Chloroflexi in the activated sludge. Additionally, fluorescence in situ hybridization experiments showed that diverse filamentous Caldilineae inhabit the activated sludge of municipal WWTPs. These findings yield insight into the role of filamentous mesophilic Caldilinea in stabilizing flocs of activated sludge in a wide range of WWTPs.
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