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- Role of a Burkholderia pseudomallei Polyphosphate Kinase in an Oxidative Stress Response, Motilities, and Biofilm Formation
- Suda Tunpiboonsak , Rungrawee Mongkolrob , Kaniskul Kitudomsub , Phawatwaristh Thanwatanaying , Witcha Kiettipirodom , Yanin Tungboontina , Sumalee Tungpradabkul
- J. Microbiol. 2010;48(1):63-70. Published online March 11, 2010
- DOI: https://doi.org/10.1007/s12275-010-9138-5
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- 44 Scopus
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Abstract
- Burkholderia pseudomallei, a motile and rod Gram-negative bacterium, is the causative agent of melioidosis. The bacterium is an intracellular pathogen and that motility is generally crucial for their survival in a natural environment and for systemic infection inside a host. We report here a role of B. pseudomallei polyphosphate kinase in virulence, such as an oxidative stress response, motilities and biofilm formation. The polyphosphate kinase (ppk) mutant is susceptible to hydrogen peroxide in an oxidative stress condition, unable to perform swimming, swarming motilities, and has lower density biofilm forming capacity than the wild-type strain. We also demonstrated that both polyphosphate kinase and motile flagella are essential and independently involved in biofilm formation. The B. pseudomallei flagellin (fliC) mutant and B. mallei, a nonmotile species, are shown to produce higher density biofilm formation than the ppk mutant, but less than wild type B. pseudomallei.
Journal Article
- Characterization of a Spontaneous Avirulent Mutant of Legionella pneumophila Serogroup 6: Evidence of DotA and Flagellin Involvement in the Loss of Virulence
- Maria Scaturro , Stefania Meschini , Giuseppe Arancia , Fontana Stefano , Maria Luisa Ricci
- J. Microbiol. 2009;47(6):768-773. Published online February 4, 2010
- DOI: https://doi.org/10.1007/s12275-009-0103-0
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- 2 Crossref
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Abstract
- The pathogenesis of Legionella pneumophila mainly resides in its ability to inhibit the phagosome-lysosome fusion, which normally prevents the killing of the host cells. In order to characterize the molecular alterations that occurred in a spontaneous avirulent mutant of Legionella pneumophila serogroup 6, named Vir-, we investigated the ability of the mutant to adhere to and multiply in the WI26VA4 alveolar epithelial cell line and in human macrophages, when compared to its parental strain, Vir+. We also determined the co- localization of bacteria with LAMP-1 to gain an insight into the phagosome-lysosome fusion process. Additionally, we determined the flagellin expression and dotA nucleotide sequencing. We observed a lack of expression of flagellin and an in-frame mutation in the dotA gene. The data obtained strongly suggest the loss of virulence of the mutant could probably be due to the absence of flagellin and the dysfunctional type IV secretion system, resulting from the DotA protein being severely compromised.
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- Identification and characterization of genes, encoding the 3‐hydroxybutyrate dehydrogenase and a putative lipase, in an avirulent spontaneous Legionella pneumophila serogroup 6 mutant
Maria Scaturro, Cristina Barello, Melania De Giusti, Stefano Fontana, Federica Pinci, Maria Gabriella Giuffrida, Maria Luisa Ricci
APMIS.2015; 123(4): 330. CrossRef - Evidence of the Presence of a Functional Dot/Icm Type IV-B Secretion System in the Fish Bacterial Pathogen Piscirickettsia salmonis
Fernando A. Gómez, Jaime A. Tobar, Vitalia Henríquez, Mariel Sola, Claudia Altamirano, Sergio H. Marshall, Riccardo Manganelli
PLoS ONE.2013; 8(1): e54934. CrossRef
- Identification and characterization of genes, encoding the 3‐hydroxybutyrate dehydrogenase and a putative lipase, in an avirulent spontaneous Legionella pneumophila serogroup 6 mutant
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