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- Development of a stringent ELISA protocol to evaluate anti-viral hemorrhagic septicemia virus-specific antibodies in olive flounder Paralichthys olivaceus with improved specificity
- Hyoung Jun Kim , Jeong Su Park , Se Ryun Kwon
- J. Microbiol. 2015;53(7):481-485. Published online June 27, 2015
- DOI: https://doi.org/10.1007/s12275-015-5101-9
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- 9 Crossref
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Abstract
- Olive flounder were vaccinated with polyinosinic:polycytidylic acid [Poly (I:C)] to prevent viral hemorrhagic septicemia (VHS). Vaccine efficacy was verified by detection of anti- VHS virus (VHSV) antibodies using enzyme-linked immunosorbent assay (ELISA). In the study, ELISA absorbance values of the negative control group [Poly (I:C)-MEM10] were saturated when an ELISA protocol, that includes pretreatment of the fish sera with 5% skim milk, was used. However, the saturated OD values in the negative control did not correlate with a specific immune response against VHSV, because the group showed low survival rate (only 10%) following the VHSV challenge. Also, OD values of Poly (I:C)- VHSV group were high, and the group showed high survival rate (97.5%) against VHSV challenge test. It was suggested that the high OD values were possibly due to the presence of anti-fetal bovine serum (FBS) cross-reactivity. To compensate this, we subtracted the absorbance of infectious hematopoietic necrosis (IHNV)-Ag plates from those of the VHSV-Ag plates. However, the average value for the Poly (I:C)-VHSV group (0.167) was lower than expected even though high survival rate. We used an advanced ELISA system to pre-treat fish sera with 5% skim milk and two novirhabdoviruses as capture antigens as well as 50% FBS. The corrected absorbance values for pre-treated fish sera from the negative control Poly (I:C)-MEM10 and experimental Poly (I:C)-VHSV groups averaged 0.033 and 0.579, respectively. The specific VHSV antibody response of the vaccinated group was assessed using fish sera pretreated with skim milk and FBS and by calculating the corrected absorbance values from ELISA with two novirhabdovirus capture antigens.
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Citations
Citations to this article as recorded by
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K. A. S. N. Shanaka, K. P. Madushani, Myoung‐Jin Kim, Sumi Jung, Jehee Lee
Reviews in Aquaculture.2025;[Epub] CrossRef - Assessment and Performance of Pooled Serum Samples for Monitoring Farm-Level Immunity in Tilapia Infected with Tilapia Lake Virus
Jidapa Yamkasem, Puntanat Tattiyapong, Ian A. Gardner, Win Surachetpong
Viruses.2025; 17(7): 877. CrossRef - Development of an indirect ELISA for detection of the adaptive immune response of black carp (Mylopharyngodon piceus)
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Lena Hammerlund Teige, Subramani Kumar, Grethe M. Johansen, Øystein Wessel, Niccolò Vendramin, Morten Lund, Espen Rimstad, Preben Boysen, Maria K. Dahle
Frontiers in Immunology.2019;[Epub] CrossRef - Production of a monoclonal antibody against of muskellunge (Esox masquinongy) IgM heavy chain and its use in development of an indirect ELISA for titrating circulating antibodies against VHSV-IVB
Mohamed Faisal, Isaac F. Standish, Mary Ann Vogelbein, Elena V. Millard, Stephen L. Kaattari
Fish & Shellfish Immunology.2019; 88: 464. CrossRef - Phylogenetic analysis and duplex RT-PCR detection of viral hemorrhagic septicemia virus in olive flounder (Paralichthys olivaceus) from Korea
Jee Youn Hwang, Seongdo Lee, Thanthrige Thiunuwan Priyathilaka, Hyerim Yang, Hyukjae Kwon, Mun Gyeong Kwon, Seong Don Hwang, Myoung-Jin Kim, Jehee Lee
Aquaculture.2018; 484: 242. CrossRef - Herpesvirus Infection Induces both Specific and Heterologous Antiviral Antibodies in Carp
Julio M. Coll
Frontiers in Immunology.2018;[Epub] CrossRef - Validation of a serum neutralization test for detection of antibodies specific to cyprinid herpesvirus 3 in infected common and koi carp (Cyprinus carpio)
J Cabon, L Louboutin, J Castric, S Bergmann, G Bovo, M Matras, O Haenen, N J Olesen, T Morin
Journal of Fish Diseases.2017; 40(5): 687. CrossRef - The amino-terminal domain of ORF149 of koi herpesvirus is preferentially targeted by IgM from carp populations surviving infection
F. Torrent, A. Villena, P. A. Lee, W. Fuchs, S. M. Bergmann, J. M. Coll
Archives of Virology.2016; 161(10): 2653. CrossRef
- Virus Infection Models: Zebrafish as an Infection Model to Study Immune Landscape During Viral Hemorrhagic Septicemia Virus (VHSV) Infection
- Distribution of Marine Birnavirus in Cultured Olive Flounder Paralichthys olivaceus in Korea
- Sung-Ju Jung , Seok-Ryel Kim , Il-Yong Joung , Shin-Ichi Kitamura , Hee-Taek Ceong , Myung-Joo Oh
- J. Microbiol. 2008;46(3):265-273. Published online July 5, 2008
- DOI: https://doi.org/10.1007/s12275-008-0004-7
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- 20 Crossref
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Abstract
PDF - Surveys of marine birnavirus (MABV) were undertaken in cultured olive flounder Paralichthys olivaceus from the south and west coastal areas and Jeju in Korea during the period January 1999 to April 2007. MABV was detected in all seasons from the fry, juveniles and adult fish from the areas examined. Evident cytopathic effects of the virus including rounding and cell lysis were observed in chinook salmon embryo (CHSE-214) and rainbow trout gonad (RTG-2) cells, but not in fathead minnow (FHM) and epithelial papilloma of carp (EPC) cells. Nucleotide sequences of the VP2/NS junction region of the Korean isolates showed 97.8%~100% similarity, and they belonged to the same genogroup. Cross neutralization tests with serotype-specific rabbit antisera against MABV strains exhibited a close antigenic relationships between strains, and were distinct from infectious pancreatic necrosis virus (IPNV) strains. Coinfection of MABV with bacteria (Streptococcus iniae, Vibrio spp.) and viruses (nervous necrosis virus, lymphocystis disease virus, viral hemorrhagic septicemia virus) was observed.
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Citations
Citations to this article as recorded by- Co-infection of viral nervous necrosis (VNN) and bacterial infection outbreak in cage cultured cobia fingerlings
P. Rameshkumar, C. Balachandran, S. Vairamuthu, G. Dhinakar Raj, J. John Kirubaharan, K. Nagarajan, B.M. Nazeera, P. Ezhil Praveena, K.P. Jithendran
Aquaculture.2024; 578: 740047. CrossRef - Establishment and characterization of a cell line from the brain of the Japanese flounder (Paralichthys olivaceus) and its application in the study of viral infection
Zhaodi Sun, Yuqin Ren, Yitong Zhang, Yucong Yang, Guixing Wang, Zhongwei He, Yufeng Liu, Wei Cao, Yufen Wang, Yuanshuai Fu, Jilun Hou
Aquaculture.2023; 562: 738825. CrossRef - Isolation and characterization of nidovirus and bacterial co-infection from cultured turbot (Scophthalmus maximus) in China
Yitong Zhang, Chunguang Gong, Yaxian Zhao, Guixing Wang, Yufeng Liu, Zhongwei He, Yuqin Ren, Wei Cao, Haitao Zhao, Yufen Wang, Jilun Hou
Aquaculture.2022; 561: 738652. CrossRef - Infectious hematopoietic necrosis virus enhanced infectious pancreatic necrosis virus multiplication during co-infection in Chinook salmon embryo cell lines
Liming Xu, Jingzhuang Zhao, Miao Liu, Guangming Ren, Shi Cheng Han, Yizhi Shao, Yongsheng Cao, Hongbai Liu, Tongyan Lu
Aquaculture.2021; 531: 735898. CrossRef - Experimental infection of brown trout (Salmo trutta), zebrafi sh (Danio rerio), and swan mussel (Anodonta cygnea) with infectious pancreatic necrosis virus (IPVN)
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Agricultural Science and Practice.2020; 7(3): 31. CrossRef - Establishment and characterization of a new cell line derived from the fin of olive flounder (Paralichthys olivaceus)
Hee Jae Choi, Ji Hoon Lee, Hye Jin Jung, Ju Noh Lee, Kwang Il Kim, Jun-Hwan Kim, Yue Jai Kang
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Jing Chen, Xinyu Toh, Jasmine Ong, Yahui Wang, Xuan-Hui Teo, Bernett Lee, Pui-San Wong, Denyse Khor, Shin-Min Chong, Diana Chee, Alvin Wee, Yifan Wang, Mee-Keun Ng, Boon-Huan Tan, Taoqi Huangfu
Virology Journal.2019;[Epub] CrossRef - Co‐infection of rainbow trout (Oncorhynchus mykiss) with infectious hematopoietic necrosis virus and Flavobacterium psychrophilum
Jie Ma, Timothy J. Bruce, Luke P. Oliver, Kenneth D. Cain
Journal of Fish Diseases.2019; 42(7): 1065. CrossRef - Virus–Bacteria Interactions: Implications and Potential for the Applied and Agricultural Sciences
Matthew Moore, Lee-Ann Jaykus
Viruses.2018; 10(2): 61. CrossRef - Gene expression profiles alteration after infection of virus, bacteria, and parasite in the Olive flounder (Paralichthys olivaceus)
Gyu-Hwi Nam, Anshuman Mishra, Jeong-An Gim, Hee-Eun Lee, Ara Jo, Dahye Yoon, Ahran Kim, Woo-Jin Kim, Kung Ahn, Do-Hyung Kim, Suhkmann Kim, Hee-Jae Cha, Yung Hyun Choi, Chan-Il Park, Heui-Soo Kim
Scientific Reports.2018;[Epub] CrossRef - Complete genomic sequence of an infectious pancreatic necrosis virus isolated from rainbow trout (Oncorhynchus mykiss) in China
Feng Ji, Jing-zhuang Zhao, Miao Liu, Tong-yan Lu, Hong-bai Liu, Jiasheng Yin, Li-Ming Xu
Virus Genes.2017; 53(2): 215. CrossRef - Invitromatics, invitrome, and invitroomics: introduction of three new terms for in vitro biology and illustration of their use with the cell lines from rainbow trout
Niels C. Bols, Phuc H. Pham, Vivian R. Dayeh, Lucy E. J. Lee
In Vitro Cellular & Developmental Biology - Animal.2017; 53(5): 383. CrossRef - Surveillance of aquatic animal viruses in seawater and shellfish in Korea
Kwang Il Kim, Woo Ju Kwon, Young Chul Kim, Myoung-Sug Kim, Suhee Hong, Hyun Do Jeong
Aquaculture.2016; 461: 17. CrossRef - The impact of co-infections on fish: a review
Mohamed H. Kotob, Simon Menanteau-Ledouble, Gokhlesh Kumar, Mahmoud Abdelzaher, Mansour El-Matbouli
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Diseases of Aquatic Organisms.2014; 109(1): 9. CrossRef - A survey of fish viruses isolated from wild marine fishes from the coastal waters of southern Korea
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Journal of Veterinary Diagnostic Investigation.2013; 25(6): 750. CrossRef - Evaluation of rapid and sensitive reverse transcription loop-mediated isothermal amplification method for detecting Infectious pancreatic necrosis virus in chum salmon (Oncorhynchus keta)
Rungkarn Suebsing, Myung-Joo Oh, Jeong-Ho Kim
Journal of Veterinary Diagnostic Investigation.2011; 23(4): 704. CrossRef - Detection and Genetic Analysis of Aquabirnaviruses in Subclinically Infected Aquarium Fish
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Current Opinion in Virology.2011; 1(1): 73. CrossRef - Distribution of marine birnavirus (MABV) in marine organisms from Okinawa, Japan, and a unique sequence variation of the VP2/NS region
Manami Inaba, Satoru Suzuki, Shin-Ichi Kitamura, Norichika Kumazawa, Hiroshi Kodama
The Journal of Microbiology.2009; 47(1): 76. CrossRef
- Co-infection of viral nervous necrosis (VNN) and bacterial infection outbreak in cage cultured cobia fingerlings
- Genetic Analysis of the VP2/NS Junction Region on Segment A of Marine Birnavirus Isolated from Cultured Flounder Paralichthys olivaceous
- Seong-Joon Joh , Jeong-Ho Kim , Gang-Joon Heo
- J. Microbiol. 2000;38(1):44-47.
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Abstract
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- The cDNA of VP2/NS junction region on segment A of the marine birnavirus (MBV) isolated from flounder (DS strain) was amplified using the reverse transcription (RT)-polymerase chain reaction (PCR). Its cDNA nucleotide and deduced amino acid sequences were analyzed, and compared with the reference strains of MBV and infectious pancreatic necrosis virus (IPNV). Analyses of the nucleotide and deduced amino acid sequences revealed that the DS strain is very similar to the reference strains of MBV, distant from the IPNV.
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