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- The small RNA RsaF regulates the expression of secreted virulence factors in Staphylococcus aureus Newman
- Niralee Patel , Mrinalini Nair
- J. Microbiol. 2021;59(10):920-930. Published online September 23, 2021
- DOI: https://doi.org/10.1007/s12275-021-1205-6
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- 5 Web of Science
- 5 Crossref
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Abstract
- The pathogenesis of Staphylococcus aureus, from local infections to systemic dissemination, is mediated by a battery of virulence factors that are regulated by intricate mechanisms, which include regulatory proteins and small RNAs (sRNAs) as key regulatory molecules. We have investigated the involvement of sRNA RsaF, in the regulation of pathogenicity genes hyaluronate lyase (hysA) and serine proteaselike protein D (splD), by employing S. aureus strains with disruption and overexpression of rsaF. Staphylococcus aureus strain with disruption of rsaF exhibited marked down-regulation of hysA transcripts by 0.2 to 0.0002 fold, and hyaluronate lyase activity by 0.2–0.1 fold, as well as increased biofilm formation, during growth from log phase to stationery phase. These mutants also displayed down-regulation of splD transcripts by 0.8 to 0.005 fold, and reduced activity of multiple proteases by zymography. Conversely, overexpression of rsaF resulted in a 2- to 4- fold increase in hysA mRNA levels and hyaluronidase activity. Both hysA and splD mRNAs demonstrated an increased stability in RsaF+ strains. In silico RNA-RNA interaction indicated a direct base pairing of RsaF with hysA and splD mRNAs, which was established in electrophoretic mobility shift assays. The findings demonstrate a positive regulatory role for small RNA RsaF in the expression of the virulence factors, HysA and SplD.
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Citations
Citations to this article as recorded by
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Jun Ren, Nuong Thi Nong, Phuong N. Lam Vo, Hyang-Mi Lee, Dokyun Na
ACS Synthetic Biology.2024; 13(10): 3256. CrossRef - A comprehensive review on microbial hyaluronan-degrading enzymes: from virulence factors to biotechnological tools
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Applied Biochemistry and Microbiology.2022; 58(S1): S47. CrossRef - A Review of Biofilm Formation of Staphylococcus aureus and Its Regulation Mechanism
Qi Peng, Xiaohua Tang, Wanyang Dong, Ning Sun, Wenchang Yuan
Antibiotics.2022; 12(1): 12. CrossRef - Thirty Years of sRNA-Mediated Regulation in Staphylococcus aureus: From Initial Discoveries to In Vivo Biological Implications
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International Journal of Molecular Sciences.2022; 23(13): 7346. CrossRef
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