Journal Articles
- Vaginal Microbiome Dysbiosis is Associated with the Different Cervical Disease Status
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Yingying Ma , Yanpeng Li , Yanmei Liu , Le Cao , Xiao Han , Shujun Gao , Chiyu Zhang
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J. Microbiol. 2023;61(4):423-432. Published online April 3, 2023
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DOI: https://doi.org/10.1007/s12275-023-00039-3
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7
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Abstract
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Vaginal microbiome composition was demonstrated to be associated with cervical disease. The colonization characteristics
of vaginal microbes and their association with the different cervical disease status, especially cervical cancer (CC), are
rarely investigated. In this cross-sectional study, we characterized the vaginal microbiome of women with different status of
cervical diseases, including 22 NV + (normal tissue with HPV infection), low-grade squamous intraepithelial lesion (LSIL,
n = 45), high-grade squamous intraepithelial lesion (HSIL, n = 36) and CC (n = 27) using bacterial 16S DNA sequencing.
Thirty HPV-negative women with normal tissue were used as the control group. We found that higher diversity of microbiome
with gradual depletion of Lactobacillus, especially L. crispatus, was associated with the severity of cervical disease.
High-risk HPV16 infection was associated with higher microbiome diversity and depletion of Lactobacillus in high-grade
cervical diseases (i.e. HSIL and CC). The CC group was characterized by higher levels of Fannyhessea vaginae, Prevotella,
Bacteroides, Finegoldia, Vibrio, Veillonella, Peptostreptococcus, and Dialister. Co-occurrence network analyses showed that
negative correlations were exclusively observed between Lactobacillus and other bacteria, and almost all non-Lactobacillus
bacteria were positively correlated with each other. In particular, the most diverse and complex co-occurrence network of
vaginal bacteria, as well as a complete loss of L. crispatus, was observed in women with CC. Logistic regression model
identified HPV16 and Lactobacillus as significant risk and protective factors for CC, respectively. These results suggest that
specific Lactobacillus species (e.g. L. crispatus and L. iners) can be used as important markers to target prevention measures
prioritizing HPV16-infected women and other hrHPV-infected women for test, vaccination and treat initiatives.
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- Vaginal Microbiome and Pregnancy Complications: A Review
Angeliki Gerede, Konstantinos Nikolettos, Eleftherios Vavoulidis, Chrysoula Margioula-Siarkou, Stamatios Petousis, Maria Giourga, Panagiotis Fotinopoulos, Maria Salagianni, Sofoklis Stavros, Konstantinos Dinas, Nikolaos Nikolettos, Ekaterini Domali
Journal of Clinical Medicine.2024; 13(13): 3875. CrossRef - Advancements in the Vaginal Microenvironment and Regression of High-Risk Human Papillomavirus
Na He, Cunjian Yi, Qingsong Zeng, Wumei Jing, Wenrong He
Indian Journal of Microbiology.2024;[Epub] CrossRef - Research Progress on Related Factors of Cervical High-Grade Squamous Intraepithelial Lesions
红颖 王
Advances in Clinical Medicine.2023; 13(12): 20536. CrossRef - Role of the vaginal microbiome in miscarriage: exploring the relationship
Marwa Saadaoui, Parul Singh, Osman Ortashi, Souhaila Al Khodor
Frontiers in Cellular and Infection Microbiology.2023;[Epub] CrossRef
- Gamma-glutamyltransferase of Helicobacter pylori alters the proliferation, migration, and pluripotency of mesenchymal stem cells by affecting metabolism and methylation status
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Zeyu Wang , Weijun Wang , Huiying Shi , Lingjun Meng , Xin Jiang , Suya Pang , Mengke Fan , Rong Lin
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J. Microbiol. 2022;60(6):627-639. Published online April 18, 2022
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DOI: https://doi.org/10.1007/s12275-022-1575-4
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56
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7
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5
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Abstract
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Virulence factor gamma-glutamyltransferase (GGT) of H.
pylori consumes glutamine (Gln) in the stomach to decrease
the tricarboxylic acid metabolite alpha-ketoglutarate (α-kg)
and alter the downstream regulation of α-kg as well as cellular
biological characteristics. Our previous research indicated
that under H. pylori infection, mesenchymal stem cells
(MSCs) migrated to the stomach and participated in gastric
cancer (GC) development either by differentiating into epithelial
cells or promoting angiogenesis. However, how MSCs
themselves participate in H. pylori-indicated GC remains
unclear. Therefore, a GGT knockout H. pylori strain (Hp-
KS-1) was constructed, and downstream histone H3K9 and
H3K27 methylation and the PI3K/AKT signaling pathway
of α-kg were detected using Western blotting. The biological
characteristics of MSCs were also examined. An additive α-kg
supplement was also added to H. pylori-treated MSCs to investigate
alterations in these aspects. Compared to the control
and Hp-KS-1 groups, H. pylori-treated MSCs reduced Gln
and α-kg, increased H3K9me3 and H3K27me3, activated the
PI3K-AKT signaling pathway, and promoted the proliferation,
migration, self-renewal, and pluripotency of MSCs. The
addition of α-kg rescued the H. pylori-induced alterations.
Injection of MSCs to nude mice resulted in the largest tumors
in the H. pylori group and significantly reduced tumor sizes
in the Hp-KS-1 and α-kg groups. In summary, GGT of H.
pylori affected MSCs by interfering with the metabolite α-kg
to increase trimethylation of histone H3K9 and H3K27, activating
the PI3K/AKT signaling pathway, and promoting
proliferation, migration, self-renewal, and pluripotency in tumorigenesis,
elucidating the mechanisms of MSCs in GC
development.
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- Gamma-glutamyl transferase secreted by Helicobacter pylori promotes the development of gastric cancer by affecting the energy metabolism and histone methylation status of gastric epithelial cells
Xin Jiang, Weijun Wang, Zeyu Wang, Zhe Wang, Huiying Shi, Lingjun Meng, Suya Pang, Mengke Fan, Rong Lin
Cell Communication and Signaling.2024;[Epub] CrossRef - Design of a Helicobacter pylori multi-epitope vaccine based on immunoinformatics
Man Cui, Xiaohui Ji, Fengtao Guan, Guimin Su, Lin Du
Frontiers in Immunology.2024;[Epub] CrossRef - Gastric cancer and mesenchymal stem cell-derived exosomes: from pro-tumorigenic effects to anti-cancer vehicles
Maryam Dolatshahi, Ahmad Reza Bahrami, Qaiser Iftikhar Sheikh, Mohsen Ghanbari, Maryam M. Matin
Archives of Pharmacal Research.2024; 47(1): 1. CrossRef - Mesenchymal Stem Cell-Derived Exosomes Modulate Angiogenesis in Gastric Cancer
Fawzy Akad, Veronica Mocanu, Sorin Nicolae Peiu, Viorel Scripcariu, Bogdan Filip, Daniel Timofte, Florin Zugun-Eloae, Magdalena Cuciureanu, Monica Hancianu, Teodor Oboroceanu, Laura Condur, Radu Florin Popa
Biomedicines.2023; 11(4): 1031. CrossRef - Helicobacter pylori and Its Role in Gastric Cancer
Victor E. Reyes
Microorganisms.2023; 11(5): 1312. CrossRef
Research Support, Non-U.S. Gov't
- Clades of γ-Glutamyltransferases (GGTs) in the Ascomycota and Heterologous Expression of Colletotrichum graminicola CgGGT1, a Member of the Pezizomycotina-only GGT Clade
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Marco H. Bello , Lynn Epstein
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J. Microbiol. 2013;51(1):88-99. Published online March 2, 2013
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DOI: https://doi.org/10.1007/s12275-013-2434-0
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Abstract
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Gamma-glutamyltransferase (GGT, EC 2.3.2.2) cleaves the γ-glutamyl linkage in glutathione (GSH). Ascomycetes in either the Saccharomycotina or the Taphrinomycotina have one to three GGTs, whereas members of the Pezizomycotina have two to four GGTs. A Bayesian analysis indicates there are three well-supported main clades of GGTs in the Ascomycota. 1) A Saccharomycotina and a Taphrinomycotinaspecific GGT sub-clade form a yeast main clade. This clade has the three relatively well-characterized fungal GGTs: (Saccharomyces cerevisiae CIS2 and Schizosaccharomyces pombe Ggt1 and Ggt2) and most of its members have all 14 of the highly conserved and critical amino acids that are found in GGTs in the other kingdoms. 2) In contrast, a main clade (GGT3) differs in 11 of the 14 highly conserved amino acids that are found in GGTs in the other kingdoms. All of the 44 Pezizomycotina analyzed have either one or two GGT3s. 3) There is a Pezizomycotina-only GGT clade that has two wellsupported sub-clades (GGT1 and GGT2); this clade differs in only two of the 14 highly conserved amino acids found in GGTs in the other kingdoms. Because the Pezizomycotina GGTs differ in apparently critical amino acids from the crosskingdom consensus, a putative GGT from Colletotrichum graminicola, a member of the Pezizomycotina, was cloned and the protein product was expressed as a secreted protein in Pichia pastoris. A GGT enzyme assay of the P. pastoris supernatant showed that the recombinant protein was active, thereby demonstrating that CgGGT1 is a bona fide GGT.