Journal of Microbiology

Research Support, Non-U.S. Gov'ts

Molecular Characterization of Atoxigenic Aspergillus flavus Isolates Collected in China: Dandan Wei , Lu Zhou , Jonathan Nimal Selvaraj , Chushu Zhang , Fuguo Xing , Yueju Zhao , Yan Wang , Yang Liu; J. Microbiol. 2014;52(7):559-565. Published online May 30, 2014; DOI: https://doi.org/10.1007/s12275-014-3629-8

53 View
0 Download
25 Crossref

Aspergillus flavus strains were isolated from peanut fields of Liaoning, Shandong, Hubei and Guangdong Provinces in China, and identified through phenotypic and molecular approaches. Of the 323 A. flavus strains isolated, 76 strains did not produce aflatoxins detectable by UPLC. The incidence of atoxigenic A. flavus strains decreased with increase in temperature and increased with increase in latitude in different geographical locations. Amplification of all the aflatoxin genes in the aflatoxin gene cluster in the atoxigenic isolates showed that there were 25 deletion patterns (A-Y), with 22 deletion patterns identified for the first time. Most of the atoxigenic A. flavus isolates with gene deletions (97%) had deletions in at least one of the four genes (aflT, nor-1, aflR, and hypB), indicating that these four genes could be targeted for rapid identification of atoxigenic strains. The atoxigenic isolates with gene deletions, especially the isolates with large deletions, are potential candidates for aflatoxin control.

Citations

Citations to this article as recorded by

Aflatoxin profiles of Aspergillus flavus isolates in Sudanese fungal rhinosinusitis
Shaoqin Zhou, Mawahib A I Ismail, Vishukumar Aimanianda, G Sybren de Hoog, Yingqian Kang, Sarah A Ahmed
Medical Mycology.2024;[Epub] CrossRef
Mitigation of aflatoxin contamination of maize, groundnut, and sorghum by commercial biocontrol products in farmers’ fields across Burkina Faso, Mali, Niger, and Togo
Saïdou Bonkoungou, Karim Dagno, Adamou Basso, Tedihou Ekanao, Joseph Atehnkeng, Daniel Agbetiameh, Adama Neya, Mahama Toure, Assiata Tiendrebeogo, Mamadou Konate, Bibata Outani, Matieyedou Konlambigue, Kenneth A. Callicott, Peter J. Cotty, Ibnou Dieng, Ti
CABI Agriculture and Bioscience.2024;[Epub] CrossRef
The biodiversity of Aspergillus flavus in stored rice grain leads to a decrease in the overall aflatoxin B1 production in these species
Song Tan, Fang Ma, Yajie Wu, Yuancheng Xu, Ajuan Niu, Yuping Chen, Guangyu Wang, Weifen Qiu
International Journal of Food Microbiology.2023; 406: 110416. CrossRef
Biocontrol efficacy of atoxigenic Aspergillus flavus strains against aflatoxin contamination in peanut field in Guangdong province, South China
Firew Tafesse Mamo, Bo Shang, Jonathan Nimal Selvaraj, Yongquan Zheng, Yang Liu
Mycology.2022; 13(2): 143. CrossRef
Selection of Atoxigenic Aspergillus flavus for Potential Use in Aflatoxin Prevention in Shandong Province, China
Jia Xu, Peng Wang, Zehua Zhou, Peter John Cotty, Qing Kong
Journal of Fungi.2021; 7(9): 773. CrossRef
Distribution of Aspergillus Fungi and Recent Aflatoxin Reports, Health Risks, and Advances in Developments of Biological Mitigation Strategies in China
Firew Tafesse Mamo, Birhan Addisie Abate, Yougquan Zheng, Chengrong Nie, Mingjun He, Yang Liu
Toxins.2021; 13(10): 678. CrossRef
Co-Inoculation of Aflatoxigenic and Non-Aflatoxigenic Strains of Aspergillus flavus to Assess the Efficacy of Non-Aflatoxigenic Strains in Growth Inhibition and Aflatoxin B1 Reduction
Rahim Khan, Farinazleen Mohamad Ghazali, Nor Ainy Mahyudin, Nik Iskandar Putra Samsudin
Agriculture.2021; 11(3): 198. CrossRef
Insight into the substrate-dependent anti-aflatoxigenic effects of nanosized ZnO film: Electron transfer directed oxidative stress mechanisms
Yichuan Zhang, Miya Zhou, Huanmei Cheng, Songyi Luo, Qi Sun
Colloids and Surfaces B: Biointerfaces.2021; 207: 111997. CrossRef
Deciphering the origin ofAspergillus flavusNRRL21882, the active biocontrol agent of Afla‐Guard®
P.‐K. Chang, T.D. Chang, K. Katoh
Letters in Applied Microbiology.2021; 72(5): 509. CrossRef
Inhibition of the aflatoxin‐producing fungus Aspergillus flavus by a plasma jet system
Winai Intanon, Norrapon Vichiansan, Komgrit Leksakul, Dheerawan Boonyawan, Jaturong Kumla, Nakarin Suwannarach, Saisamorn Lumyong
Journal of Food Processing and Preservation.2021;[Epub] CrossRef
Molecular profile of non-aflatoxigenic phenotype in native strains of Aspergillus flavus
K. Raksha Rao, A. V. Vipin, G. Venkateswaran
Archives of Microbiology.2020; 202(5): 1143. CrossRef
Field efficacy of two atoxigenic biocontrol products for mitigation of aflatoxin contamination in maize and groundnut in Ghana
Daniel Agbetiameh, Alejandro Ortega-Beltran, Richard T. Awuah, Joseph Atehnkeng, Abuelgasim Elzein, Peter J. Cotty, Ranajit Bandyopadhyay
Biological Control.2020; 150: 104351. CrossRef
Mechanism of inhibition of aflatoxin synthesis by non-aflatoxigenic strains of Aspergillus flavus
K. Raksha Rao, A.V. Vipin, G. Venkateswaran
Microbial Pathogenesis.2020; 147: 104280. CrossRef
Ethanol Inhibits Aflatoxin B1 Biosynthesis in Aspergillus flavus by Up-Regulating Oxidative Stress-Related Genes
Yaoyao Ren, Jing Jin, Mumin Zheng, Qingli Yang, Fuguo Xing
Frontiers in Microbiology.2020;[Epub] CrossRef
The bZIP transcription factor Afap1 mediates the oxidative stress response and aflatoxin biosynthesis in Aspergillus flavus
Xuanli Guan, Yueju Zhao, Xiao Liu, Bo Shang, Fuguo Xing, Lu Zhou, Yan Wang, Chushu Zhang, Deepak Bhatnagar, Yang Liu
Revista Argentina de Microbiología.2019; 51(4): 292. CrossRef
The Development of a qPCR Assay to Measure Aspergillus flavus Biomass in Maize and the Use of a Biocontrol Strategy to Limit Aflatoxin Production
Alfred Mitema, Sheila Okoth, Suhail M. Rafudeen
Toxins.2019; 11(3): 179. CrossRef
Extrinsic harmful residues in Chinese herbal medicines: types, detection, and safety evaluation
Cong-min Liu, Jia-an Qin, Xiao-wen Dou, Mei-hua Yang, Xiao-bo Sun
Chinese Herbal Medicines.2018; 10(2): 117. CrossRef
Versicolorin A is a potential indicator of aflatoxin contamination in the granary-stored corn
Shu-Yao Zhang, Hao Wang, Min Yang, Dong-Sheng Yao, Chun-Fang Xie, Da-Ling Liu
Food Additives & Contaminants: Part A.2018; 35(5): 972. CrossRef
Isolation and characterization of Aspergillus flavus strains in China
Firew Tafesse Mamo, Bo Shang, Jonathan Nimal Selvaraj, Yan Wang, Yang Liu
Journal of Microbiology.2018; 56(2): 119. CrossRef
Large-Scale Comparative Analysis of Eugenol-Induced/Repressed Genes Expression in Aspergillus flavus Using RNA-seq
Cong Lv, Ping Wang, Longxue Ma, Mumin Zheng, Yang Liu, Fuguo Xing
Frontiers in Microbiology.2018;[Epub] CrossRef
Prevalence of Aflatoxin Contamination in Maize and Groundnut in Ghana: Population Structure, Distribution, and Toxigenicity of the Causal Agents
D. Agbetiameh, A. Ortega-Beltran, R. T. Awuah, J. Atehnkeng, P. J. Cotty, R. Bandyopadhyay
Plant Disease.2018; 102(4): 764. CrossRef
Cinnamaldehyde inhibits fungal growth and aflatoxin B1 biosynthesis by modulating the oxidative stress response of Aspergillus flavus
Qi Sun, Bo Shang, Ling Wang, Zhisong Lu, Yang Liu
Applied Microbiology and Biotechnology.2016; 100(3): 1355. CrossRef
A strain ofAspergillus flavusfrom China shows potential as a biocontrol agent for aflatoxin contamination
Lu Zhou, Dan-Dan Wei, Jonathan Nimal Selvaraj, Bo Shang, Chu-Shu Zhang, Fu-Guo Xing, Yue-Ju Zhao, Yan Wang, Yang Liu
Biocontrol Science and Technology.2015; 25(5): 583. CrossRef
High sequence variations in the region containing genes encoding a cellular morphogenesis protein and the repressor of sexual development help to reveal origins of Aspergillus oryzae
Perng-Kuang Chang, Leslie L. Scharfenstein, Cesar D. Solorzano, Hamed K. Abbas, Sui-Sheng T. Hua, Walker A. Jones, Robert M. Zablotowicz
International Journal of Food Microbiology.2015; 200: 66. CrossRef
Diversity, Saccharification Capacity, and Toxigenicity Analyses of Fungal Isolates in Nuruk
Min Sik Kim, Sinil Kim, Byeong-Seok Ha, Hye-Young Park, Seong-Yeol BaeK, Soo-Hwan Yeo, Hyeon-Su Ro
The Korean Journal of Mycology.2014; 42(3): 191. CrossRef

Identification and Characterization of Ectoine Biosynthesis Genes and Heterologous Expression of the ectABC Gene Cluster from Halomonas sp. QHL1, a Moderately Halophilic Bacterium Isolated from Qinghai Lake: Derui Zhu , Jian Liu , Rui Han , Guoping Shen , Qifu Long , Xiaoxing Wei , Deli Liu; J. Microbiol. 2014;52(2):139-147. Published online February 1, 2014; DOI: https://doi.org/10.1007/s12275-014-3389-5

44 View
0 Download
16 Crossref

Abstract

The moderately halophilic bacterium Halomonas sp. QHL1 was identified as a member of the genus Halomonas by 16S rRNA gene sequencing. HPLC analysis showed that strain QHL1 synthesizes ectoine in its cytoplasm. The genes involved in the ectoine biosynthesis pathway were identified on the chromosome in the order ectABC. Subsequently, the ectB gene from this strain was amplified by PCR, and the entire ectABC gene cluster (3,580 bp) was cloned using genome walking. Analysis showed that the ectA (579 bp), ectB (1269 bp), and ectC (390 bp) genes were organized in a single transcriptional unit and were predicted to encode three peptides of 21.2 kDa, 46.4 kDa, and 14.7 kDa, respectively. Two putative promoters, a δ70-dependent promoter and a δ38-controlled promoter, as well as several conserved motifs with unknown function were identified. Individual ectA, ectB, and ectC genes, and the entire ectABC gene cluster were inserted into the expression plasmid pET-28a(+) to generate the recombinant plasmids pET-28a(+)-ectA, pET-28a(+)-ectB, pET-28a(+)-ectC and pET-28a(+)-ectABC, respectively. Heterologous expression of these proteins in Escherichia coli BL21 (DE3) was confirmed by SDS-PAGE. The recombinant E. coli strain BL21 (pET-28a (+)-ectABC) displayed a higher salt tolerance than native E. coli cells but produced far less ectoine than the wild-type QHL1 strain.

Citations

Citations to this article as recorded by

Biotechnological production of ectoine: current status and prospects
Jun Chen, Deliang Qiao, Tao Yuan, Yeyuan Feng, Pengjun Zhang, Xuejun Wang, Li Zhang
Folia Microbiologica.2024; 69(2): 247. CrossRef
Comparative genomic analysis of Halomonas campaniensis wild-type and ultraviolet radiation-mutated strains reveal genomic differences associated with increased ectoine production
Zhibo Wang, Yongzhen Li, Xiang Gao, Jiangwa Xing, Rong Wang, Derui Zhu, Guoping Shen
International Microbiology.2023; 26(4): 1009. CrossRef
Comparative Genome Analysis of a Novel Alkaliphilic Actinobacterial Species Nesterenkonia haasae
Shuang Wang, Lei Sun, Manik Prabhu Narsing Rao, Bao‑zhu Fang, Wen‑jun Li
Polish Journal of Microbiology.2022; 71(3): 453. CrossRef
Whole genome sequencing of the halophilic Halomonas qaidamensis XH36, a novel species strain with high ectoine production
Tiantian Zhang, Tianqi Cui, Yaning Cao, Yongzhen Li, Fenghui Li, Derui Zhu, Jiangwa Xing
Antonie van Leeuwenhoek.2022; 115(4): 545. CrossRef
Identification and characterization of an ectoine biosynthesis gene cluster from Aestuariispira ectoiniformans sp. nov., isolated from seawater
Ji Young Kang, Binna Lee, Jeong Ah Kim, Min-Soo Kim, Chul Ho Kim
Microbiological Research.2022; 254: 126898. CrossRef
Genome Sequence of Streptomyces sp. Strain GQFP Isolated from Soil Near the Roots of Pharmaceutical Plant Elaeagnus pungens
Jie Zhu, David A. Baltrus
Microbiology Resource Announcements.2022;[Epub] CrossRef
Enhanced production of ectoine from methane using metabolically engineered Methylomicrobium alcaliphilum 20Z
Sukhyeong Cho, Yun Seo Lee, Hanyu Chai, Sang Eun Lim, Jeong Geol Na, Jinwon Lee
Biotechnology for Biofuels and Bioproducts.2022;[Epub] CrossRef
Ectoine Production Using Novel Heterologous EctABCS. salarius from Marine Bacterium Salinicola salarius
Yue Su, Wenting Peng, Tong Wang, Yanhui Li, Luyu Zhao, Xinyu Wang, Ying Li, Ling Lin
Applied Sciences.2021; 11(15): 6873. CrossRef
High ectoine production by an engineered Halomonas hydrothermalis Y2 in a reduced salinity medium
Qi Zhao, Shannan Li, Peiwen Lv, Simian Sun, Cuiqing Ma, Ping Xu, Haijun Su, Chunyu Yang
Microbial Cell Factories.2019;[Epub] CrossRef
Analysis of Zobellella denitrificans ZD1 draft genome: Genes and gene clusters responsible for high polyhydroxybutyrate (PHB) production from glycerol under saline conditions and its CRISPR-Cas system
Yu-Wei Wu, Shih-Hung Yang, Myung Hwangbo, Kung-Hui Chu, Chih-Horng Kuo
PLOS ONE.2019; 14(9): e0222143. CrossRef
Engineering the Salt-Inducible Ectoine Promoter Region of Halomonas elongata for Protein Expression in a Unique Stabilizing Environment
Lisa Stiller, Erwin Galinski, Elisabeth Witt
Genes.2018; 9(4): 184. CrossRef
New Alpiniamides From Streptomyces sp. IB2014/011-12 Assembled by an Unusual Hybrid Non-ribosomal Peptide Synthetase Trans-AT Polyketide Synthase Enzyme
Constanze Paulus, Yuriy Rebets, Josef Zapp, Christian Rückert, Jörn Kalinowski, Andriy Luzhetskyy
Frontiers in Microbiology.2018;[Epub] CrossRef
Metabolic engineering of Escherichia coli for L-tryptophan production
V. B. Panichkin, V. A. Livshits, I. V. Biryukova, S. V. Mashko
Applied Biochemistry and Microbiology.2016; 52(9): 783. CrossRef
Draft Genome Sequence of Halomonas elongata Strain K4, an Endophytic Growth-Promoting Bacterium Enhancing Salinity Tolerance In Planta
Feras F. Lafi, Juan S. Ramirez-Prado, Intikhab Alam, Vladimir B. Bajic, Heribert Hirt, Maged M. Saad
Genome Announcements.2016;[Epub] CrossRef
High production of ectoine from aspartate and glycerol by use of whole-cell biocatalysis in recombinant Escherichia coli
Yong-Zhi He, Jiao Gong, Hai-Ying Yu, Yong Tao, Shan Zhang, Zhi-Yang Dong
Microbial Cell Factories.2015;[Epub] CrossRef
Design of an ectoine-responsive AraC mutant and its application in metabolic engineering of ectoine biosynthesis
Wei Chen, Shan Zhang, Peixia Jiang, Jun Yao, Yongzhi He, Lincai Chen, Xiwu Gui, Zhiyang Dong, Shuang-Yan Tang
Metabolic Engineering.2015; 30: 149. CrossRef

Cloning and Characterization of the Gene Cluster for Biosynthesis of Ectoine from Nesterenkonia halobia DSM 20541: Bo Zhang , Xin Bao , Lei Wang , Su Sheng Yang; J. Microbiol. 2008;46(3):309-318. Published online July 5, 2008; DOI: https://doi.org/10.1007/s12275-008-0001-x

37 View
0 Download
7 Scopus

Abstract: The ectABC genes encoding the biosynthesis of ectoine were identified from Nesterenkonia halobia DSM 20541. The intergenic regions of the ectABC genes from N. halobia DSM 20541 were more loosely spaced than those that had been reported before. The amino acid sequence deduced from ectABC of the strain was highly homologous to the EctABC of Brevibacterium linens BL2 (EctA 50%, EctB 70%, and EctC 68% identities). The osmoprotection of ectABC was studied in the Escherichia coli KNabc and E. coli XL1-Blue. The results revealed that ectABC could shorten the lag phase and enhance the final OD600 of E. coli XL1-Blue in MM63 medium containing 0.68 M NaCl, and could initiate KNabc growth in 0.2 M NaCl. Ectoine was proven to be accumulated in E. coli KNabc/pGEM-Nect using HPLC-UV, and validated by LC-MSD-Trap-VL.

Genetic Characterization of the Escherichia coli O66 Antigen and Functional Identification of its wzy Gene: Jiansong Cheng , Bin Liu , David A. Bastin , Weiqing Han , Lei Wang , Lu Feng; J. Microbiol. 2007;45(1):69-74.; DOI: https://doi.org/2488 [pii]

40 View
0 Download

Abstract: Escherichia coli is a clonal species, and occurs as both commensal and pathogenic strains, which are normally classified on the basis of their O, H, and K antigens. The O-antigen (O-specific polysaccharide), which consists of a series of oligosaccharide (O-unit) repeats, contributes major antigenic variability to the cell surface. The O-antigen gene cluster of E. coli O66 was sequenced in this study. The genes putatively responsible for the biosynthesis of dTDP-6-deoxy-L-talose and GDP-mannose, as well as those responsible for the transfer of sugars and for O-unit processing were identified based on their homology. The function of the wzy gene was confirmed by the results of a mutation test. Genes specific for E. coli O66 were identified via PCR screening against representatives of 186 E. coli and Shigella O type strains. The comparison of intergenic sequences located between galF and the O-antigen gene cluster in a range of E. coli and Shigella showed that this region may perform an important function in the homologous recombination of the O-antigen gene clusters.

First
Prev
Page of 1
Next
Last

Search