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Research Support, Non-U.S. Gov't
- NOTE] A Rapid PCR-Based Approach for Molecular Identification of Filamentous Fungi
- Yuanyuan Chen , Bernard A. Prior , Guiyang Shi , Zhengxiang Wang
- J. Microbiol. 2011;49(4):675-679. Published online September 2, 2011
- DOI: https://doi.org/10.1007/s12275-011-0525-3
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- 13 Scopus
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Abstract
- In this study, a novel rapid and efficient DNA extraction method based on alkaline lysis, which can deal with a large number of filamentous fungal isolates in the same batch, was established. The filamentous fungal genomic DNA required only 20 min to prepare and can be directly used as a template for PCR amplification. The amplified internal transcribed spacer regions were easy to identify by analysis. The extracted DNA also can be used to amplify other protein-coding genes for fungal identification. This method can be used for rapid systematic identification of filamentous fungal isolates.
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