MSK
Search
- Page Path
- HOME > Search
Research Support, Non-U.S. Gov't
- Transcriptional Regulation of fksA, a β-1,3-Glucan Synthase Gene, by the APSES Protein StuA during Aspergillus nidulans Development
- Bum-Chan Park , Yun-Hee Park , Soohyun Yi , Yu Kyung Choi , Eun-Hye Kang , Hee-Moon Park
- J. Microbiol. 2014;52(11):940-947. Published online October 31, 2014
- DOI: https://doi.org/10.1007/s12275-014-4517-y
- 52 View
- 0 Download
- 12 Crossref
-
Abstract
- The temporal and spatial regulation of β-1,3-glucan synthesis plays an important role in morphogenesis during fungal growth and development. Northern blot analysis showed that the transcription of fksA, the gene encoding β-1,3-glucan synthase in Aspergillus nidulans, was cell-cycle-dependent and increased steadily over the duration of the vegetative period, but its overall expression during the asexual and sexual stages was fairly constant up until the time of transcription cessation. In an A. nidulans strain mutated in the eukaryotic bHLH-like APSES transcription factor stuA1, the transcriptional level of fksA, and consequently the content of alkali-insoluble cell wall β-glucan, significantly increased at the conidial chain formation and maturation stage. Electrophoretic mobility shift assays revealed that StuA was bound to StREs (StuA Response Elements) on the fksA promoter region. Promoter analysis with sGFP-fusion constructs also indicated the negative regulation of fksA expression by StuA, especially during asexual development. Taken together, these data suggest that StuA plays an important role in cell wall biogenesis during the development of A. nidulans, by controlling the transcription level of fksA.
-
Citations
Citations to this article as recorded by
- Survival Factor A (SvfA) Contributes to Aspergillus nidulans Pathogenicity
Joo-Yeon Lim, Ye-Eun Jung, Hye-Eun Hwang, Cheol-Hee Kim, Nese Basaran-Akgul, Sri Harshini Goli, Steven P. Templeton, Hee-Moon Park
Journal of Fungi.2023; 9(2): 143. CrossRef - Potential utility of endophytic Bacillus altitudinis strain P32-3 as a biocontrol agent for the postharvest prevention of sweet potato black rot
Yong-Jing Zhang, Xiao-Ying Cao, Yu-Jie Chen, Hao Cong, Yi-Ming Wang, Ji-Hong Jiang, Lu-Dan Li
Biological Control.2023; 186: 105350. CrossRef - Survival factor SvfA plays multiple roles in differentiation and is essential for completion of sexual development in Aspergillus nidulans
Joo-Yeon Lim, Eun-Hye Kang, Yun-Hee Park, Jun-Ho Kook, Hee-Moon Park
Scientific Reports.2020;[Epub] CrossRef - Expression Analysis of Cell Wall-Related Genes in the Plant Pathogenic Fungus Drechslera teres
Aurélie Backes, Jean-Francois Hausman, Jenny Renaut, Essaid Ait Barka, Cédric Jacquard, Gea Guerriero
Genes.2020; 11(3): 300. CrossRef - Dynamic Transcriptomic and Phosphoproteomic Analysis During Cell Wall Stress in Aspergillus nidulans
Cynthia Chelius, Walker Huso, Samantha Reese, Alexander Doan, Stephen Lincoln, Kelsi Lawson, Bao Tran, Raj Purohit, Trevor Glaros, Ranjan Srivastava, Steven D. Harris, Mark R. Marten
Molecular & Cellular Proteomics.2020; 19(8): 1310. CrossRef - Molecular Dialogues between Early Divergent Fungi and Bacteria in an Antagonism versus a Mutualism
Olga A. Lastovetsky, Lev D. Krasnovsky, Xiaotian Qin, Maria L. Gaspar, Andrii P. Gryganskyi, Marcel Huntemann, Alicia Clum, Manoj Pillay, Krishnaveni Palaniappan, Neha Varghese, Natalia Mikhailova, Dimitrios Stamatis, T. B. K. Reddy, Chris Daum, Nicole Sh
mBio.2020;[Epub] CrossRef - The Basic-Region Helix-Loop-Helix Transcription Factor DevR Significantly Affects Polysaccharide Metabolism in Aspergillus oryzae
Miao Zhuang, Zhi-Min Zhang, Long Jin, Bao-Teng Wang, Yasuji Koyama, Feng-Jie Jin, Maia Kivisaar
Applied and Environmental Microbiology.2019;[Epub] CrossRef - The Dual-Specificity LAMMER Kinase Affects Stress-Response and Morphological Plasticity in Fungi
Joo-Yeon Lim, Hee-Moon Park
Frontiers in Cellular and Infection Microbiology.2019;[Epub] CrossRef - The APSES transcription factor Vst1 is a key regulator of development in microsclerotium‐ and resting mycelium‐producing Verticillium species
Jorge L. Sarmiento‐Villamil, Nicolás E. García‐Pedrajas, Lourdes Baeza‐Montañez, María D. García‐Pedrajas
Molecular Plant Pathology.2018; 19(1): 59. CrossRef - Essential APSES Transcription Factors for Mycotoxin Synthesis, Fungal Development, and Pathogenicity in Aspergillus flavus
Guangshan Yao, Feng Zhang, Xinyi Nie, Xiuna Wang, Jun Yuan, Zhenhong Zhuang, Shihua Wang
Frontiers in Microbiology.2017;[Epub] CrossRef - A Transcriptome Meta-Analysis Proposes Novel Biological Roles for the Antifungal Protein AnAFP in Aspergillus niger
Norman Paege, Sascha Jung, Paul Schäpe, Dirk Müller-Hagen, Jean-Paul Ouedraogo, Caroline Heiderich, Johanna Jedamzick, Benjamin M. Nitsche, Cees A. van den Hondel, Arthur F. Ram, Vera Meyer, Kap-Hoon Han
PLOS ONE.2016; 11(11): e0165755. CrossRef - Role of LAMMER Kinase in Cell Wall Biogenesis during Vegetative Growth ofAspergillus nidulans
Yu Kyung Choi, Eun-Hye Kang, Hee-Moon Park
Mycobiology.2014; 42(4): 422. CrossRef
- Survival Factor A (SvfA) Contributes to Aspergillus nidulans Pathogenicity
- Antifungal activities of peptides with the sequence 10-17 of magainin 2 at the N-termini against aspergillus fumigatus
- Lee, Myung Kyu , Lee, Dong Gun , Shin, Song Yub , Lee, Sung Gu , Kang, Joo Hyun , Hahm, Kyung Soo
- J. Microbiol. 1996;34(3):274-278.
- 38 View
- 0 Download
-
Abstract
- Two peptides, MA-inv AND MA-ME, with the sequence 10-17 of maganin 2 at their-N-termini were designed and synthesized. The peptides had higher antifungal activities against Aspergilus fumigatus without hemolytic activities. The minimal inhibition concentratory (MIC) values of both peptides against A. fumigatus were 5 ㎍/ml, whereas those of the native peptides, magainin 2 and melittin, were 10㎍/ml. At 3 ㎍/ml, MA-inv and MA-ME inhibited the mycelium growth of A. fumigatus by 94.6% and 97.3% respectively, whereas magainin 2 and melittin inhibited by 62.2% and 32.4, respectively. MA-inv showed up to 80% inhibition of (1, 3)-β-D-glucan synthase activity of A. fumigatus. The peptides also showed up to 80% inhibition of (1, 3)-β-D glucan synthase activity of A. fumigatus. The peptides also showed antifungal activities for other fungi of Aspergillus sp. However, the antibiotic activities of MA-ME against Escherichia coli, Bacillus subtilis and Fusarium oxysporum were more effective than those of MA-inv, suggesting that the C-terminal sequences of MA-inv and MA-ME may also influence their antibiotic activities. These results suggest that the N-terminal sequence of the designed peptides, KKFGKAFV, is important for their antifungal activities against A. fumigatus and their C- terminal sequences are related to the organism selectivity.
First
Prev
TOP
