MSK
Search
- Page Path
- HOME > Search
Journal Article
- Construction of a genetic linkage map and QTL mapping of agronomic traits in Auricularia auricula-judae
- Li-Xin Lu , Fang-Jie Yao , Peng Wang , Ming Fang , You-Min Zhang , Wei-Tong Zhang , Xiang-Hui Kong , Jia Lu
- J. Microbiol. 2017;55(10):792-799. Published online September 28, 2017
- DOI: https://doi.org/10.1007/s12275-017-7241-6
- 46 View
- 0 Download
- 13 Crossref
-
Abstract
- Auricularia auricula-judae is a traditional edible fungus that is cultivated widely in China. In this study, a genetic linkage map for A. auricula-judae was constructed using a mapping population consisting of 138 monokaryons derived from a hybrid strain (A119-5). The monokaryotic parent strains A14-5 and A18-119 were derived from two cultivated varieties, A14 (Qihei No. 1) and A18 (Qihei No. 2), respectively. In total, 130 simple sequence repeat markers were mapped. These markers were developed using the whole genome sequence of A. auricula-judae and amplified in A14-5, A18- 119, and the mapping population. The map consisted of 11 linkage groups (LGs) spanning 854 cM, with an average interval length of 6.57 cM. A testcross population was derived from crossing between the monokaryon A184-57 (from the wild strain A184 as a tester strain) and the mapping population. Important agronomic trait-related QTLs, including mycelium growth rate on potato dextrose agar for the mapping population, mycelium growth rate on potato dextrose agar and sawdust for the testcross population, growth period (days from inoculation to fruiting body harvesting), and yield for the testcross population, were identified using the composite interval mapping method. Six mycelium growth raterelated QTLs were identified on LG1 and LG4, two growth period-related QTLs were identified on LG2, and three yieldrelated QTLs were identified on LG2 and LG6. The results showed no linkage relationship between mycelium growth rate and growth period. The present study provides a foundation for locating genes for important agronomic characteristics in A. auricula-judae in the future.
-
Citations
Citations to this article as recorded by
- Exploring the potential of black fungus, Auricularia auricula, as a feed additive in African catfish, Clarias gariepinus, farming
Lee Seong Wei, Alvin Amos Adrian Susin, Albaris B. Tahiluddin, Liew Vui Kien, Wendy Wee
Heliyon.2024; 10(13): e33810. CrossRef - Evaluation of Drought Tolerance and Trehalose Response in Auricularia heimuer
Jian Sun, Fangjie Yao, Lixin Lu, Youmin Zhang, Ming Fang, Xiaoxu Ma, Kaisheng Shao, Xu Sun
Horticulturae.2024; 10(12): 1312. CrossRef -
Medicinal Value, Genetic Diversity, and Genetic Relationship Analysis of Auricularia cornea (Agaricomycetes) Based on ITS, ISSR, and SRAP Markers
Ping Du, Hong-Yan He, Ni-Ya Wu, Tianxu Cao, Bao-Kai Cui
International Journal of Medicinal Mushrooms.2024; 26(5): 43. CrossRef - Velvet Family Members Regulate Pigment Synthesis of the Fruiting Bodies of Auricularia cornea
Xiaoxu Ma, Lixin Lu, Youmin Zhang, Ming Fang, Kaisheng Shao, Xu Sun, Fangjie Yao, Peng Wang
Journal of Fungi.2023; 9(4): 412. CrossRef - The polysaccharides from Auricularia auricula alleviate non-alcoholic fatty liver disease via modulating gut microbiota and bile acids metabolism
Yifan Shu, Yujie Huang, Wei Dong, Xia Fan, Yi Sun, Guijie Chen, Xiaoxiong Zeng, Hong Ye
International Journal of Biological Macromolecules.2023; 246: 125662. CrossRef - Detection of quantitative trait loci underlying fruiting body and yield-related traits in Hericium erinaceus
Wenbing Gong, Xiaoya Song, Chunliang Xie, Yingjun Zhou, Zuohua Zhu, Chao Xu, Yuande Peng
Scientia Horticulturae.2022; 293: 110729. CrossRef - Analysis of the Genome Sequence of Strain GiC-126 of Gloeostereum incarnatum with Genetic Linkage Map
Wan-Zhu Jiang, Fang-Jie Yao, Ming Fang, Li-Xin Lu, You-Min Zhang, Peng Wang, Jing-Jing Meng, Jia Lu, Xiao-Xu Ma, Qi He, Kai-Sheng Shao, Asif Ali Khan, Yun-Hui Wei
Mycobiology.2021; 49(4): 406. CrossRef - SNP-Based Genetic Linkage Map and Quantitative Trait Locus Mapping Associated with the Agronomically Important Traits of Hypsizygus marmoreus
Youn-Lee Oh, In-Geol Choi, Kab-Yeul Jang, Min-Seek Kim, Min ji Oh, Ji-Hoon Im
Mycobiology.2021; 49(6): 589. CrossRef - Genetic linkage map construction and quantitative trait loci mapping of agronomic traits in Gloeostereum incarnatum
Wan-Zhu Jiang, Fang-Jie Yao, Li-Xin Lu, Ming Fang, Peng Wang, You-Min Zhang, Jing-Jing Meng, Jia Lu, Xiao-Xu Ma, Qi He, Kai-Sheng Shao
Journal of Microbiology.2021; 59(1): 41. CrossRef - Polysaccharides from Auricularia auricula: Preparation, structural features and biological activities
Nana Chen, Hao Zhang, Xin Zong, Siyu Li, Jiaojiao Wang, Yizhen Wang, Mingliang Jin
Carbohydrate Polymers.2020; 247: 116750. CrossRef - Genome Sequence Analysis of Auricularia heimuer Combined with Genetic Linkage Map
Ming Fang, Xiaoe Wang, Ying Chen, Peng Wang, Lixin Lu, Jia Lu, Fangjie Yao, Youmin Zhang
Journal of Fungi.2020; 6(1): 37. CrossRef - A Resequencing-Based Ultradense Genetic Map of Hericium erinaceus for Anchoring Genome Sequences and Identifying Genetic Loci Associated With Monokaryon Growth
Wenbing Gong, Chunliang Xie, Yingjun Zhou, Zuohua Zhu, Yahui Wang, Yuande Peng
Frontiers in Microbiology.2020;[Epub] CrossRef - Comparative transcriptome analysis reveals relationship of three major domesticated varieties of Auricularia auricula-judae
Yuhui Zhao, Liang Wang, Dongshan Zhang, Rong Li, Tianyou Cheng, Yibi Zhang, Xueju Liu, Gary Wong, Yuguo Tang, Hui Wang, Shan Gao
Scientific Reports.2019;[Epub] CrossRef
- Exploring the potential of black fungus, Auricularia auricula, as a feed additive in African catfish, Clarias gariepinus, farming
Research Support, Non-U.S. Gov't
- Biochemical Properties and Physiological Roles of NADP-Dependent Malic Enzyme in Escherichia coli
- Baojuan Wang , Peng Wang , Enxia Zheng , Xiangxian Chen , Hanjun Zhao , Ping Song , Ruirui Su , Xiaoning Li , Guoping Zhu
- J. Microbiol. 2011;49(5):797-802. Published online November 9, 2011
- DOI: https://doi.org/10.1007/s12275-011-0487-5
- 43 View
- 0 Download
- 21 Crossref
-
Abstract
- Malic enzymes catalyze the reversible oxidative decarboxylation of L-malate using NAD(P)+ as a cofactor. NADP-dependent malic enzyme (MaeB) from Escherichia coli MG1655 was expressed and purified as a fusion protein. The molecular weight of MaeB was about 83 kDa, as determined by SDS-PAGE. The recombinant MaeB showed a maximum activity at pH 7.8 and 46°C. MaeB activity was dependent on the presence of Mn2+ but was strongly inhibited by Zn2+. In order to understand the physiological roles, recombinant E. coli strains (icdNADP/ΔmaeB and icdNAD/ΔmaeB) containing NADP-dependent isocitrate dehydrogenase (IDH), or engineered NAD-dependent IDH with the deletion of the maeB gene, were constructed using homologous recombination. During growth on acetate, icdNAD/ΔmaeB grew poorly, having a growth rate only 60% that of the wild-type strain (icdNADP). Furthermore, icdNADP/ΔmaeB exhibited a 2-fold greater adaptability to acetate than icdNAD/ΔmaeB, which may be explained by more NADPH production for biosynthesis in icdNADP/ΔmaeB due to its NADP-dependent IDH. These results indicated that MaeB was important for NADPH production for bacterial growth on acetate. We also observed that MaeB activity was significantly enhanced (7.83-fold) in icdNAD, which was about 3-fold higher than that in icdNADP, when switching from glucose to acetate. The marked increase of MaeB activity was probably induced by the shortage of NADPH in icdNAD. Evidently, MaeB contributed to the NADPH generation needed for bacterial growth on two carbon compounds.
-
Citations
Citations to this article as recorded by- TA-Cloning for Diabetes Treatment: Expressing Corynebacterium Malic Enzyme Gene in E. coli
Somayeh Farahmand, Saber SamadiAfshar, Ladan Hosseini
Current Microbiology.2024;[Epub] CrossRef - Recent advances, challenges and metabolic engineering strategies in the biosynthesis of 3‐hydroxypropionic acid
Bo Liang, Guannan Sun, Xinping Zhang, Qingjuan Nie, Yukun Zhao, Jianming Yang
Biotechnology and Bioengineering.2022; 119(10): 2639. CrossRef - Metabolic engineering of Halomonas elongata: Ectoine secretion is increased by demand and supply driven approaches
Karina Hobmeier, Martin Oppermann, Natalie Stasinski, Andreas Kremling, Katharina Pflüger-Grau, Hans Jörg Kunte, Alberto Marin-Sanguino
Frontiers in Microbiology.2022;[Epub] CrossRef - Improved production of 2′-fucosyllactose in engineered Saccharomyces cerevisiae expressing a putative α-1, 2-fucosyltransferase from Bacillus cereus
Mingyuan Xu, Xiangfeng Meng, Weixin Zhang, Yu Shen, Weifeng Liu
Microbial Cell Factories.2021;[Epub] CrossRef - One stone two birds: Biosynthesis of 3-hydroxypropionic acid from CO2 and syngas-derived acetic acid in Escherichia coli
Ningyu Lai, Yuanchan Luo, Peng Fei, Peng Hu, Hui Wu
Synthetic and Systems Biotechnology.2021; 6(3): 144. CrossRef -
Transcriptomics and Methylomics Study on the Effect of Iodine-Containing Drug FS-1 on
Escherichia Coli
ATCC BAA-196
Ilya S Korotetskiy, Ardak B Jumagaziyeva, Sergey V Shilov, Tatyana V Kuznetsova, Auyes N Myrzabayeva, Zhanar A Iskakbayeva, Aleksandr I Ilin, Monique Joubert, Setshaba Taukobong, Oleg N Reva
Future Microbiology.2021; 16(14): 1063. CrossRef - Enzymatic characterization of blood lymphocytes in various clinical and pathogenetic variants of respiratory allergy
A. M. Lazareva, O. A. Kolenchukova, S. V. Smirnova
Medical Immunology (Russia).2021; 23(1): 107. CrossRef - The Protein-Protein Interaction Network Reveals a Novel Role of the Signal Transduction Protein PII in the Control of c-di-GMP Homeostasis in Azospirillum brasilense
Edileusa C. M. Gerhardt, Erick Parize, Fernanda Gravina, Flávia L. D. Pontes, Adrian R. S. Santos, Gillize A. T. Araújo, Ana C. Goedert, Alysson H. Urbanski, Maria B. R. Steffens, Leda S. Chubatsu, Fabio O. Pedrosa, Emanuel M. Souza, Karl Forchhammer, Ele
mSystems.2020;[Epub] CrossRef - Metabolic changes in peripheral blood lymphocytes from children with recurrent respiratory infections
L. M. Kurtasova, N. A. Shakina, T. V. Lubnina
Russian Journal of Infection and Immunity.2020; 10(3): 515. CrossRef - The NADP-dependent malic enzyme MaeB is a central metabolic hub controlled by the acetyl-CoA to CoASH ratio
Luciano F. Huergo, Gillize A.T. Araújo, Adrian S.R. Santos, Edileusa C.M. Gerhardt, Fabio O. Pedrosa, Emanuel M. Souza, Karl Forchhammer
Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics.2020; 1868(9): 140462. CrossRef - Rediscovering Acetate Metabolism: Its Potential Sources and Utilization for Biobased Transformation into Value-Added Chemicals
Hyun Gyu Lim, Ji Hoon Lee, Myung Hyun Noh, Gyoo Yeol Jung
Journal of Agricultural and Food Chemistry.2018; 66(16): 3998. CrossRef - Enhanced performance of the methylerythritol phosphate pathway by manipulation of redox reactions relevant to IspC, IspG, and IspH
Jia Zhou, Liyang Yang, Chonglong Wang, Eui-Sung Choi, Seon-Won Kim
Journal of Biotechnology.2017; 248: 1. CrossRef - Rational design of a synthetic Entner–Doudoroff pathway for improved and controllable NADPH regeneration
Chiam Yu Ng, Iman Farasat, Costas D. Maranas, Howard M. Salis
Metabolic Engineering.2015; 29: 86. CrossRef - Metabolic network capacity of Escherichia coli for Krebs cycle-dependent proline hydroxylation
Eleni Theodosiou, Oliver Frick, Bruno Bühler, Andreas Schmid
Microbial Cell Factories.2015;[Epub] CrossRef - NADPH-generating systems in bacteria and archaea
Sebastiaan K. Spaans, Ruud A. Weusthuis, John van der Oost, Servé W. M. Kengen
Frontiers in Microbiology.2015;[Epub] CrossRef - Central metabolic responses to the overproduction of fatty acids in Escherichia coli based on 13C‐metabolic flux analysis
Lian He, Yi Xiao, Nikodimos Gebreselassie, Fuzhong Zhang, Maciek R. Antoniewicz, Yinjie J. Tang, Lifeng Peng
Biotechnology and Bioengineering.2014; 111(3): 575. CrossRef - Optimal cofactor swapping can increase the theoretical yield for chemical production in Escherichia coli and Saccharomyces cerevisiae
Zachary A. King, Adam M. Feist
Metabolic Engineering.2014; 24: 117. CrossRef - Engineering of NADPH regenerators in Escherichia coli for enhanced biotransformation
Won-Heong Lee, Myoung-Dong Kim, Yong-Su Jin, Jin-Ho Seo
Applied Microbiology and Biotechnology.2013; 97(7): 2761. CrossRef - Expression characteristics of the maeA and maeB genes by extracellular malate and pyruvate in Escherichia coli
Irisappan Ganesh, Sambandam Ravikumar, Si Jae Park, Seung Hwan Lee, Soon Ho Hong
Korean Journal of Chemical Engineering.2013; 30(7): 1443. CrossRef - Optimizing Cofactor Specificity of Oxidoreductase Enzymes for the Generation of Microbial Production Strains—OptSwap
Zachary A. King, Adam M. Feist
Industrial Biotechnology.2013; 9(4): 236. CrossRef - Investigating the effects of perturbations to pgi and eno gene expression on central carbon metabolism in Escherichia coli using 13 C metabolic flux analysis
Yuki Usui, Takashi Hirasawa, Chikara Furusawa, Tomokazu Shirai, Natsuko Yamamoto, Hirotada Mori, Hiroshi Shimizu
Microbial Cell Factories.2012;[Epub] CrossRef
- TA-Cloning for Diabetes Treatment: Expressing Corynebacterium Malic Enzyme Gene in E. coli
- Effects of Genetically Different 2.4-D-degradative Plasmids on Degradation Phenotype and Competitiveness of Soil Microorganisms
- Hong, Seok Myeong , Ahn, Young Joon , Park, Yong Keun , Min, Kyung Hee , Kim, Chi Kyung , Ka, Jong Ok
- J. Microbiol. 1995;33(3):208-214.
- 33 View
- 0 Download
-
Abstract
- The effects of various 2, 4-D-degradative plasmids on the axenic growth patterns, the degradation phenotypes, and the competitiveness of different host bacteria were evaluated in liquid cultures; the organisms and plasmids used were Alcaligenes eutrophus JMP134/pJP4, Alcaligenes paradoxus/p2811, Pseudomonas pickettii/p712, pJP4, and p712 or p 2811 exhibited very different restriction fragment profiles in restriction endonuclease digests. These plasmids were transferred to the recipients (P. cepacia and Alcaligenes JMP228) at relatively high frequencies ranging from 8.9 × 10^-3 to 1.6 × 10^-5 per donor cell. In the axenic liquid cultures the fast-growing strains, such as P. pseudomallei/p745 and P. cepacia/pJP4, exhibited short lag periods, high specific growth rates, and high relative fitness coefficients, while the slow-growing strains, such as P. pickettii/p712 and A. paradoxus/p2811, had long lag periods, low specific growth rates, and low relative fitness coefficients. Depending on the type of plasmid containing the genes for the 2, 4-D pathway, some transconjugants exhibited intermediate growth patterns between the fast-growing strains and the slow-growing strains. The plasmid and plasmid-host interactions determined specific growth rate and lag time, respectively, which were shown to be principal determinants of competitiveness among the strains, but relative fitness coefficient derived from the axenic culture was not always predictive for the mixed culture condition.
First
Prev
TOP
