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- Guinea pig complement potently measures vibriocidal activity of human antibodies in response to cholera vaccines
- Kyoung Whun Kim , Soyoung Jeong , Ki Bum Ahn , Jae Seung Yang , Cheol-Heui Yun , Seung Hyun Han
- J. Microbiol. 2017;55(12):973-978. Published online December 7, 2017
- DOI: https://doi.org/10.1007/s12275-017-7478-0
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Abstract
- The vibriocidal assay using guinea pig complement is widely used for the evaluation of immune responses to cholera vaccines in human clinical trials. However, it is unclear why guinea pig complement has been used over human complement in the measurement of vibriocidal activity of human sera and there have not been comparison studies for the use of guinea pig complement over those from other species. Therefore, we comparatively investigated the effects of complements derived from human, guinea pig, rabbit, and sheep on vibriocidal activity. Complements from guinea pig, rabbit, and human showed concentration-dependent vibriocidal activity in the presence of quality control serum antibodies. Of these complements, guinea pig complement was the most sensitive and effective over a wide concentration range. When the vibriocidal activity of complements was measured in the absence of serum antibodies, human, sheep, and guinea pig complements showed vibriocidal activity up to 40-fold, 20- fold, and 1-fold dilution, respectively. For human pre- and post-vaccination sera, the most potent vibriocidal activity was observed when guinea pig complement was used. In addition, the highest fold-increases between pre- and post- vaccinated sera were obtained with guinea pig complement. Furthermore, human complement contained a higher amount of V. cholerae- and its lipopolysaccharide-specific antibodies than guinea pig complement. Collectively, these results suggest that guinea pig complements are suitable for vibriocidal assays due to their high sensitivity and effectiveness to human sera.
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- Immunogenicity and protective efficacy of a live, oral cholera vaccine formulation stored outside-the-cold-chain for 140 days
Tew Hui Xian, Kurunathan Sinniah, Chan Yean Yean, Venkateskumar Krishnamoorthy, Mohd Baidi Bahari, Manickam Ravichandran, Guruswamy Prabhakaran
BMC Immunology.2020;[Epub] CrossRef - A high-throughput, bead-based, antigen-specific assay to assess the ability of antibodies to induce complement activation
Stephanie Fischinger, Jonathan K. Fallon, Ashlin R. Michell, Thomas Broge, Todd J. Suscovich, Hendrik Streeck, Galit Alter
Journal of Immunological Methods.2019; 473: 112630. CrossRef - Characterization of antibody response in patients with acute and chronic chikungunya virus disease
Fatih Anfasa, Stephanie M. Lim, Susan Fekken, Robert Wever, Albert D.M.E. Osterhaus, Byron E.E. Martina
Journal of Clinical Virology.2019; 117: 68. CrossRef
- Immunogenicity and protective efficacy of a live, oral cholera vaccine formulation stored outside-the-cold-chain for 140 days
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