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- Heterologous prime-boost immunization with live SPY1 and DnaJ protein of Streptococcus pneumoniae induces strong Th1 and Th17 cellular immune responses in mice
- Yulan Qiu , Xuemei Zhang , Xinyuan Zhang , Yunjun Mo , Xiaoyu Sun , Jichao Wang , Yibing Yin , Wenchun Xu
- J. Microbiol. 2017;55(10):823-829. Published online September 28, 2017
- DOI: https://doi.org/10.1007/s12275-017-7262-1
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- 4 Crossref
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Abstract
-
diseases in children under 5-year-old. Vaccine has been used
as an indispensable strategy to prevent S. pneumoniae infection
for more than 30 years. Our previous studies confirmed
that mucosal immunization with live attenuated strain SPY1
can protect mice against nasopharyngeal colonization of S.
pneumoniae and lethal pneumococcal infection, and the
protective effects are comparable with those induced by commercially
available 23-valent polysaccharide vaccine. However,
live attenuated vaccine SPY1 needs four inoculations to
get satisfactory protective effect, which may increase the risk
of virulence recovery. It is reported that heterologous primeboost
approach is more effective than homologous primeboost
approach. In the present study, to decrease the doses
of live SPY1 and improve the safety of SPY1 vaccine, we immunized
mice with SPY1 and DnaJ protein alternately. Our
results
showed that heterologous prime-boost immunization with SPY1 and DnaJ protein could significantly reduce the colonization of S. pneumoniae in the respiratory tract of mice, and induce stronger Th1 and Th17 cellular immune responses than SPY1 alone. These results indicate heterologous prime-boost immunization method not only elicits better protective effect than SPY1 alone, but also reduces the doses of live SPY1 and decreases the risk of SPY1 vaccine. This work is the first time to study the protective efficiency with two different forms of S. pneumoniae candidate vaccine, and provides a new strategy for the development of S. pneumoniae vaccine. -
Citations
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Frontiers in Immunology.2018;[Epub] CrossRef
- Subcutaneous immunization with the fusion protein ΔA146Ply-SP0148 confers protection against Streptococcus pneumoniae infection
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