Journal Article
- In vitro disinfection efficacy and clinical protective effects of common disinfectants against acute hepatopancreatic necrosis disease (AHPND)-causing Vibrio isolates in Pacific white shrimp Penaeus vannamei
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Peizhuo Zou , Qian Yang , Hailiang Wang , Guosi Xie , Zhi Cao , Xing Chen , Wen Gao , Jie Huang
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J. Microbiol. 2020;58(8):675-686. Published online July 27, 2020
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DOI: https://doi.org/10.1007/s12275-020-9537-1
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Acute hepatopancreatic necrosis disease (AHPND) is one
of the most significant bacterial diseases in global shrimp
culture, causing severe economic losses. In the present study,
we carried out in vitro antimicrobial tests to investigate the
disinfection efficacy of 14 common disinfectants toward different
AHPND-causing Vibrio spp., including eight isolates
of V. parahaemolyticus, four isolates of V. campbellii, and
one isolate of V. owensii. Polyhexamethylene biguanidine hydrochloride
(PHMB) was revealed to possess the strongest
inhibitory activity. Through analyzing and evaluating the results
of antimicrobial tests and acute toxicity test, we selected
PHMB and hydrogen peroxide (H2O2) for further clinical
protection test. Clinical manifestations indicated that both
PHMB (2 mg/L and 4 mg/L) and H2O2 (12 mg/L) could effectively
protect juvenile Penaeus vannamei from the infection
of V. parahaemolyticus isolate Vp362 at 106 CFU/ml, and the
survival rate was over 80%. When the bacterial concentration
was reduced to 105 CFU/ml, 104 CFU/ml, and 103 CFU/ml,
the survival rate after treated by 1 mg/L PHMB was 64.44%,
93.33%, and 100%, respectively. According to the results,
PHMB and H2O2 showed a lower toxicity while a better protection
activity, particularly against a lower concentration of
the pathogens. Therefore, these two disinfectants are proved
to be promising disinfectants that can be applied to prevent
and control AHPND in shrimp culture. Moreover, the methods
of this study also provided valuable information for the
prevention of other important bacterial diseases and suggested
a reliable means for screening potential drugs in aquaculture.
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Citations
Citations to this article as recorded by

- Bactericidal Effect and Mechanism of Polyhexamethylene Biguanide (PHMB) on Pathogenic Bacteria in Marine Aquaculture
Lanting Wu, Chunyuan Wang, Yingeng Wang, Yongxiang Yu, Zheng Zhang, Cuiping Ma, Xiaojun Rong, Ling Chen, Meijie Liao, Yapeng Yang
Biology.2025; 14(5): 470. CrossRef - Comparative disinfection efficacy in aquaculture: novel methylene bis(thiocyanate) vs. conventional trichloroisocyanuric acid against Aeromonas hydrophila
Guang Yang, Ying Huang, Ning Ma, Kai Li, Xiao-mei Wang, Lian-bo Zhang, Wen-bo Yang, Wan-li Zhang, Lei Xia, Hong-Yu Zhang, Li-lai Yuan
Frontiers in Microbiology.2025;[Epub] CrossRef - Basigin binds bacteria and activates Dorsal signaling to promote antibacterial defense in Penaeus vannamei
Linwei Yang, Zi-ang Wang, Yushi Gan, Hongliang Zuo, Hengwei Deng, Shaoping Weng, Jianguo He, Xiaopeng Xu
Fish & Shellfish Immunology.2023; 142: 109123. CrossRef - Clinical protective effects of polyhexamethylene biguanide hydrochloride (PHMB) against Vibrio parahaemolyticus causing translucent post-larvae disease (VTPD) in Penaeus vannamei
Tianchang Jia, Tingting Xu, Jitao Xia, Shuang Liu, Wenqiang Li, Ruidong Xu, Jie Kong, Qingli Zhang
Journal of Invertebrate Pathology.2023; 201: 108002. CrossRef
Research Support, Non-U.S. Gov't
- Isolation and Characterization of Marine Pigmented Bacteria from Norwegian Coastal Waters and Screening for Carotenoids with UVA-Blue Light Absorbing Properties
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Marit H. Stafsnes , Kjell D Josefsen , Geir Kildahl-Andersen , Svein Valla , Trond E. Ellingsen , Per Bruheim
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J. Microbiol. 2010;48(1):16-23. Published online March 11, 2010
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DOI: https://doi.org/10.1007/s12275-009-0118-6
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251
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Microbial culture collections are important resources for isolation of natural compounds with novel properties. In this study, a culture collection of around 1,500 pigmented heterotrophic bacteria was established. The bacteria were isolated from the sea surface microlayer at different sampling sites along the mid-part of the Norwegian coast. The bacterial isolates produced pigments of various coloration (e.g. golden, yellow, red, pink and orange). Methanol extracts of sixteen isolates were characterized with LC-Diodearray-TOF mass spectrometry analysis. The number of pigments per isolate varied considerably, and a tentative identification of the pigments was performed based on UV-absorbance profile and molecular formula
assignation based on the accurate mass determination. The LC-MS analyses evealed that most of the pigments probably were carotenoids. Furthermore, we developed a high throughput LC-MS method for characterization and screening of a larger sub-fraction (300 isolates) of the culture collection. The aim was to screen and identify bacterial isolates producing carotenoids that absorb light in the UVA-Blue light. Six of the bacterial strains were selected for detailed investigation, including 16s rRNA sequencing, preparative HPLC for purification of major carotenoids and subsequent structural elucidation with NMR. Among the identified
carotenoids were zeaxanthin, nostoxanthin and sarcinaxanthin, some with novel glycosylation patterns.
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