Journal of Microbiology

Journal Articles

Adenosylhomocysteinase like 1 interacts with nonstructural 5A and regulates hepatitis C virus propagation: Yun-Sook Lim , Han N. Mai , Lap P. Nguyen , Sang Min Kang , Dongseob Tark , Soon B. Hwang; J. Microbiol. 2021;59(1):101-109. Published online December 23, 2020; DOI: https://doi.org/10.1007/s12275-021-0470-8

48 View
0 Download
3 Web of Science
3 Crossref

Hepatitis C virus (HCV) life cycle is highly dependent on cellular proteins for viral propagation. In order to identify the cellular factors involved in HCV propagation, we previously performed a protein microarray assay using the HCV nonstructural 5A (NS5A) protein as a probe. Of ~9,000 human cellular proteins immobilized in a microarray, adenosylhomocysteinase like 1 (AHCYL1) was among 90 proteins identified as NS5A interactors. Of these candidates, AHCYL1 was selected for further study. In the present study, we verified the physical interaction between NS5A and AHCYL1 by both in vitro pulldown and coimmunoprecipitation assays. Furthermore, HCV NS5A interacted with endogenous AHCYL1 in Jc1-infected cells. Both NS5A and AHCYL1 were colocalized in the cytoplasmic region in HCV-replicating cells. siRNAmediated knockdown of AHCYL1 abrogated HCV propagation. Exogenous expression of the siRNA-resistant AHCYL1 mutant, but not of the wild-type AHCYL1, restored HCV protein expression levels, indicating that AHCYL1 was required specifically for HCV propagation. Importantly, AHCYL1 was involved in the HCV internal ribosome entry site-mediated translation step of the HCV life cycle. Finally, we demonstrated that the proteasomal degradation pathway of AHCYL1 was modulated by persistent HCV infection. Collectively, these data suggest that HCV may modulate the AHCYL1 protein to promote viral propagation.

Citations

Citations to this article as recorded by

Amuvatinib Blocks SARS-CoV-2 Infection at the Entry Step of the Viral Life Cycle
Trang T. X. Huynh, Thuy X. Pham, Gun-Hee Lee, Jae-Bong Lee, Sung-Geun Lee, Dongseob Tark, Yun-Sook Lim, Soon B. Hwang, Donna M. Neumann
Microbiology Spectrum.2023;[Epub] CrossRef
Inhibition of KIF20A suppresses the replication of influenza A virus by inhibiting viral entry
Hoyeon Jeon, Younghyun Lim, In-Gu Lee, Dong-In Kim, Keun Pil Kim, So-Hee Hong, Jeongkyu Kim, Youn-Sang Jung, Young-Jin Seo
Journal of Microbiology.2022; 60(11): 1113. CrossRef
Asunaprevir, a Potent Hepatitis C Virus Protease Inhibitor, Blocks SARS-CoV-2 Propagation
Yun-Sook Lim, Lap P. Nguyen, Gun-Hee Lee, Sung-Geun Lee, Kwang-Soo Lyoo, Bumseok Kim, Soon B. Hwang
Molecules and Cells.2021; 44(9): 688. CrossRef

Comparison of virulence between matt and mucoid colonies of Klebsiella pneumoniae coproducing NDM-1 and OXA-232 isolated from a single patient: Haejeong Lee , Jin Yang Baek , So Yeon Kim , HyunJi Jo , KyeongJin Kang , Jae-Hoon Ko , Sun Young Cho , Doo Ryeon Chung , Kyong Ran Peck , Jae-Hoon Song , Kwan Soo Ko; J. Microbiol. 2018;56(9):665-672. Published online August 23, 2018; DOI: https://doi.org/10.1007/s12275-018-8130-3

48 View
0 Download
20 Crossref

Abstract

Nine Klebsiella pneumoniae isolates coproducing NDM-1 and OXA-232 carbapenemases were successively isolated from a single patient. Although they were isolated simultaneously and were isogenic, they presented different colony phenotypes (matt and mucoid). All nine isolates were resistant to most antibiotics except colistin and fosfomycin. In addition, matt-type isolates were resistant to tigecycline. No differences were detected in the cps cluster sequences, except for the insertion of IS5 in the wzb gene of two matt-type isolates. In vitro virulence assays based on production of capsular polysaccharide, biofilm formation, and resistance to human serum indicated that the mucoid-type isolates were significantly more virulent than the matt-type. In addition, mucoid-type isolates showed higher survival rates than the matt-type ones in infection experiments in the fruit fly, suggesting a higher virulence of K. pneumoniae isolates with a mucoid phenotype. To our knowledge, this is the first report of K. pneumoniae colonies with different phenotypes being isolated from the same sample. In addition, we show that virulence varies with colony phenotype. Dissemination of K. pneumoniae isolates expressing both antibiotic resistance and high virulence would constitute a great threat.

Citations

Citations to this article as recorded by

Animal models of Klebsiella pneumoniae mucosal infections
Lucas Assoni, Ana Julia Melo Couto, Brenda Vieira, Bárbara Milani, Alice Souza Lima, Thiago Rojas Converso, Michelle Darrieux
Frontiers in Microbiology.2024;[Epub] CrossRef
Development and validation of a quick, automated, and reproducible ATR FT-IR spectroscopy machine-learning model for Klebsiella pneumoniae typing
Ângela Novais, Ana Beatriz Gonçalves, Teresa G. Ribeiro, Ana R. Freitas, Gema Méndez, Luis Mancera, Antónia Read, Valquíria Alves, Lorena López-Cerero, Jesús Rodríguez-Baño, Álvaro Pascual, Luísa Peixe, Patricia J. Simner
Journal of Clinical Microbiology.2024;[Epub] CrossRef
Genetic, virulence, and antimicrobial resistance characteristics associated with distinct morphotypes in ST11 carbapenem-resistant Klebsiella pneumoniae
Tao Chen, Yuan Wang, Xiaohui Chi, Luying Xiong, Ping Lu, Xueting Wang, Yunbo Chen, Qixia Luo, Ping Shen, Yonghong Xiao
Virulence.2024;[Epub] CrossRef
Roles of crrAB two-component regulatory system in Klebsiella pneumoniae: growth yield, survival in initial colistin treatment stage, and virulence
Sun Ju Kim, Jong Hyun Shin, Hyunkeun Kim, Kwan Soo Ko
International Journal of Antimicrobial Agents.2024; 63(1): 107011. CrossRef
Clinical and Genomic Characterization of Carbapenem-Resistant Klebsiella pneumoniae with Concurrent Production of NDM and OXA-48-like Carbapenemases in Southern California, 2016–2022
Stacey Cerón, Zackary Salem-Bango, Deisy A. Contreras, Elizabeth L. Ranson, Shangxin Yang
Microorganisms.2023; 11(7): 1717. CrossRef
Improvement of transformation efficiency in hypermucoviscous Klebsiella pneumoniae using citric acid
Suyeon Park, Kwan Soo Ko
Journal of Microbiological Methods.2023; 205: 106673. CrossRef
Emergence of the clinical rdar morphotype carbapenem-resistant and hypervirulent Klebsiella pneumoniae with enhanced adaption to hospital environment
Congcong Liu, Ning Dong, Xueting Huang, Zixian Huang, Chang Cai, Jiayue Lu, Hongwei Zhou, Jingren Zhang, Yu Zeng, Fan Yang, Gongxiang Chen, Zhangqi Shen, Rong Zhang, Sheng Chen
Science of The Total Environment.2023; 889: 164302. CrossRef
Species identification, antibiotic resistance, and virulence in Enterobacter cloacae complex clinical isolates from South Korea
Michidmaral Ganbold, Jungyu Seo, Yu Mi Wi, Ki Tae Kwon, Kwan Soo Ko
Frontiers in Microbiology.2023;[Epub] CrossRef
Genomic and Phenotypic Evolution of Tigecycline-Resistant Acinetobacter baumannii in Critically Ill Patients
Jiangang Zhang, Jinru Xie, Henan Li, Zhiren Wang, Yuyao Yin, Shuyi Wang, Hongbin Chen, Qi Wang, Hui Wang, Daria Van Tyne
Microbiology Spectrum.2022;[Epub] CrossRef
Comparison of Virulence between Two Main Clones (ST11 and ST307) of Klebsiella pneumoniae Isolates from South Korea
Yun Young Cho, Jee Hong Kim, Hyunkeun Kim, Junghwa Lee, Se Jin Im, Kwan Soo Ko
Microorganisms.2022; 10(9): 1827. CrossRef
Recent progress toward the implementation of phage therapy in Western medicine
Jean-Paul Pirnay, Tristan Ferry, Grégory Resch
FEMS Microbiology Reviews.2022;[Epub] CrossRef
Two Distinct Genotypes of KPC-2-Producing Klebsiella pneumoniae Isolates from South Korea
Jee Hong Kim, Yun Young Cho, Ji Young Choi, Yu Mi Wi, Kwan Soo Ko
Antibiotics.2021; 10(8): 911. CrossRef
Increased Capsule Thickness and Hypermotility Are Traits of Carbapenem-Resistant Acinetobacter baumannii ST3 Strains Causing Fulminant Infection
Nadya Rakovitsky, Jonathan Lellouche, Debby Ben David, Sammy Frenk, Polet Elmalih, Gabriel Weber, Hadas Kon, David Schwartz, Liat Wolfhart, Elizabeth Temkin, Yehuda Carmeli
Open Forum Infectious Diseases.2021;[Epub] CrossRef
Effect of multiple, compatible plasmids on the fitness of the bacterial host by inducing transcriptional changes
Haejeong Lee, Kwan Soo Ko
Journal of Antimicrobial Chemotherapy.2021; 76(10): 2528. CrossRef
Co-introduction of plasmids harbouring the carbapenemase genes, blaNDM-1 and blaOXA-232, increases fitness and virulence of bacterial host
Haejeong Lee, Juyoun Shin, Yeun-Jun Chung, Myungseo Park, Kyeong Jin Kang, Jin Yang Baek, Dongwoo Shin, Doo Ryeon Chung, Kyong Ran Peck, Jae-Hoon Song, Kwan Soo Ko
Journal of Biomedical Science.2020;[Epub] CrossRef
Animal infection models using non‐mammals
Chikara Kaito, Kanade Murakami, Lina Imai, Kazuyuki Furuta
Microbiology and Immunology.2020; 64(9): 585. CrossRef
Change of Hypermucoviscosity in the Development of Tigecycline Resistance in Hypervirulent Klebsiella pneumoniae Sequence Type 23 Strains
Suyeon Park, Haejeong Lee, Dongwoo Shin, Kwan Soo Ko
Microorganisms.2020; 8(10): 1562. CrossRef
Epidemiology of β-Lactamase-Producing Pathogens
Karen Bush, Patricia A. Bradford
Clinical Microbiology Reviews.2020;[Epub] CrossRef
Emergence of NDM-4 and OXA-181 carbapenemase-producing Klebsiella pneumoniae
Jin Seok Kim, Chae-Kyu Hong, Sang-Hun Park, Young-Hee Jin, Sunghee Han, Hee Soon Kim, Joo-Hyun Park, Byung-Noe Bae, Ju-Young Chung, Tae-Hee Han, Jib-Ho Lee, Sang-Me Lee, Young-Hee Oh
Journal of Global Antimicrobial Resistance.2020; 20: 332. CrossRef
Evolution of Klebsiella pneumoniae with mucoid and non-mucoid type colonies within a single patient
Haejeong Lee, Juyoun Shin, Yeun-Jun Chung, Jin Yang Baek, Doo Ryeon Chung, Kyong Ran Peck, Jae-Hoon Song, Kwan Soo Ko
International Journal of Medical Microbiology.2019; 309(3-4): 194. CrossRef

[PROTOCOL] Drosophila melanogaster as a polymicrobial infection model for Pseudomonas aeruginosa and Staphylococcus aureus: Young-Joon Lee , Hye-Jeong Jang , In-Young Chung , You-Hee Cho; J. Microbiol. 2018;56(8):534-541. Published online July 25, 2018; DOI: https://doi.org/10.1007/s12275-018-8331-9

48 View
0 Download
17 Crossref

Abstract

Non-mammalian infection models have been developed over the last two decades, which is a historic milestone to understand the molecular basis of bacterial pathogenesis. They also provide small-scale research platforms for identification of virulence factors, screening for antibacterial hits, and evaluation of antibacterial efficacy. The fruit fly, Drosophila melanogaster is one of the model hosts for a variety of bacterial pathogens, in that the innate immunity pathways and tissue physiology are highly similar to those in mammals. We here present a relatively simple protocol to assess the key aspects of the polymicrobial interaction in vivo between the human opportunistic pathogens, Pseudomonas aeruginosa and Staphylococcus aureus, which is based on the systemic infection by needle pricking at the dorsal thorax of the flies. After infection, fly survival and bacteremia over time for both P. aeruginosa and S. aureus within the infected flies can be monitored as a measure of polymicrobial virulence potential. The infection takes ~24 h including bacterial cultivation. Fly survival and bacteremia are assessed using the infected flies that are monitored up to ~60 h post-infection. These methods can be used to identify presumable as well as unexpected phenotypes during polymicrobial interaction between P. aeruginosa and S. aureus mutants, regarding bacterial pathogenesis and host immunity.

Citations

Citations to this article as recorded by

Kanamycin promotes biofilm viability of MRSA strains showing extremely high resistance to kanamycin
Guangchao Yu, Teng Yi Huang, Yu Li
Microbial Pathogenesis.2024; 196: 106986. CrossRef
Design, synthesis, and evaluation of N1,N3-dialkyldioxonaphthoimidazoliums as antibacterial agents against methicillin-resistant Staphylococcus aureus
Taewoo Kim, Shin-Yae Choi, Hee-Won Bae, Hyun Su Kim, Hoon Jeon, Haejun Oh, Sung-Hoon Ahn, Jongkook Lee, Young-Ger Suh, You-Hee Cho, Seok-Ho Kim
European Journal of Medicinal Chemistry.2024; 272: 116454. CrossRef
Autolysis of Pseudomonas aeruginosa Quorum-Sensing Mutant Is Suppressed by Staphylococcus aureus through Iron-Dependent Metabolism
Shin-Yae Choi, In-Young Chung, Hee-Won Bae, You-Hee Cho
Journal of Microbiology and Biotechnology.2024; 34(4): 795. CrossRef
Drosophila melanogaster as an organism model for studying cystic fibrosis and its major associated microbial infections
Hamadoun Touré, Jean-Louis Herrmann, Sébastien Szuplewski, Fabienne Girard-Misguich, Anthony R. Richardson
Infection and Immunity.2023;[Epub] CrossRef
Drosophila melanogaster Systemic Infection Model to Study Altered Virulence during Polymicrobial Infection by Aeromonas
Alexandre Robert, Emilie Talagrand-Reboul, Maria-Jose Figueras, Raymond Ruimy, Laurent Boyer, Brigitte Lamy
Pathogens.2023; 12(3): 405. CrossRef
An iron-chelating sulfonamide identified from Drosophila-based screening for antipathogenic discovery
Yeon-Ji Yoo, In-Young Chung, Shivakumar S. Jalde, Hyun-Kyung Choi, You-Hee Cho
Virulence.2022; 13(1): 833. CrossRef
Artemisinin displays bactericidal activity via copper-mediated DNA damage
In-Young Chung, Hye-Jeong Jang, Yeon-Ji Yoo, Joonseong Hur, Hyo-Young Oh, Seok-Ho Kim, You-Hee Cho
Virulence.2022; 13(1): 149. CrossRef
Colistin-degrading proteases confer collective resistance to microbial communities during polymicrobial infections
Do-Hoon Lee, Ju-Hee Cha, Dae-Wi Kim, Kihyun Lee, Yong-Seok Kim, Hyo-Young Oh, You-Hee Cho, Chang-Jun Cha
Microbiome.2022;[Epub] CrossRef
Drosophila melanogaster as a model to study innate immune memory
Marta Arch, Maria Vidal, Romina Koiffman, Solomon Tibebu Melkie, Pere-Joan Cardona
Frontiers in Microbiology.2022;[Epub] CrossRef
An antipathogenic compound that targets the OxyR peroxide sensor in Pseudomonas aeruginosa
Hyo-Young Oh, Shivakumar S. Jalde, In-Young Chung, Yeon-Ji Yoo, Hye-Jeong Jang, Hyun-Kyung Choi, You-Hee Cho
Journal of Medical Microbiology .2021;[Epub] CrossRef
Glucose-Binding of Periplasmic Protein GltB Activates GtrS-GltR Two-Component System in Pseudomonas aeruginosa
Chenchen Xu, Qiao Cao, Lefu Lan
Microorganisms.2021; 9(2): 447. CrossRef
Silkworm model for Bacillus anthracis infection and virulence determination
Atmika Paudel, Yoshikazu Furuta, Hideaki Higashi
Virulence.2021; 12(1): 2285. CrossRef
Friends or enemies? The complicated relationship between Pseudomonas aeruginosa and Staphylococcus aureus
Deborah Bow Yue Yung, Kathleen Jean Sircombe, Daniel Pletzer
Molecular Microbiology.2021; 116(1): 1. CrossRef
Genetic and Biochemical Analysis of CodY-Mediated Cell Aggregation in Staphylococcus aureus Reveals an Interaction between Extracellular DNA and Polysaccharide in the Extracellular Matrix
Kevin D. Mlynek, Logan L. Bulock, Carl J. Stone, Luke J. Curran, Marat R. Sadykov, Kenneth W. Bayles, Shaun R. Brinsmade, Yves V. Brun
Journal of Bacteriology.2020;[Epub] CrossRef
Challenges with Wound Infection Models in Drug Development
Sandeep K. Shukla, Ajay K. Sharma, Vanya Gupta, Aman Kalonia, Priyanka Shaw
Current Drug Targets.2020; 21(13): 1301. CrossRef
Redirecting an Anticancer to an Antibacterial Hit Against Methicillin-Resistant Staphylococcus aureus
Hye-Jeong Jang, In-Young Chung, Changjin Lim, Sungkyun Chung, Bi-o Kim, Eun Sook Kim, Seok-Ho Kim, You-Hee Cho
Frontiers in Microbiology.2019;[Epub] CrossRef
Brain and bone cancer targeting by a ferrofluid composed of superparamagnetic iron-oxide/silica/carbon nanoparticles (earthicles)
Victoria M. Wu, Eric Huynh, Sean Tang, Vuk Uskoković
Acta Biomaterialia.2019; 88: 422. CrossRef

Research Support, Non-U.S. Gov't

Sequential Immunosuppressive Activities of Bacterial Secondary Metabolites from the Entomopahogenic Bacterium Xenorhabdus nematophila: Seonghyeon Eom , Youngjin Park , Yonggyun Kim; J. Microbiol. 2014;52(2):161-168. Published online February 1, 2014; DOI: https://doi.org/10.1007/s12275-014-3251-9

48 View
0 Download
37 Crossref

Abstract

The entomopathogenic bacterium Xenorhabdus nematophila secretes at least eight bacterial metabolites that play crucial roles suppressing target insect immune responses by inhibiting eicosanoid biosynthesis. We analyzed sequential changes in bacterial metabolite production during bacterial growth and analyzed their individual immunosuppressive activities against the insect host, Spodoptera exigua. X. nematophila exhibited a typical bacterial growth pattern in both insect host and culture medium, and eight metabolites were secreted at different time points. At the early growth phase (6–12 h), Ac-FGV and PHPP were detected in significant amounts in the culture broth. At this early phase, both Ac-FGV (18 μg/ml) and oxindole (110 μg/ml) levels significantly inhibited phenoloxidase and phospholipase A2 activities in S. exigua hemolymph. At the late growth phase (12–36 h), all eight metabolites were detected at significant levels (10–140 μg/ml) in the culture broth and were sufficient to induce hemocyte toxicity. These results suggest that X. nematophila sequentially produces immunosuppressive metabolites that might sequentially and cooperatively inhibit different steps of insect immune responses.

Citations

Citations to this article as recorded by

Exploring Xenorhabdus and Photorhabdus Nematode Symbionts in Search of Novel Therapeutics
Ewa Sajnaga, Waldemar Kazimierczak, Magdalena Anna Karaś, Monika Elżbieta Jach
Molecules.2024; 29(21): 5151. CrossRef
From soil to host: Discovering the tripartite interactions between entomopathogenic nematodes, symbiotic bacteria and insect pests and related challenges
Najwa Seddiqi Kallali, Abderrahman Ouijja, Khadija Goura, Salah-Eddine Laasli, Jihane Kenfaoui, Youssef Benseddik, Abdelali Blenzar, Abdelmalek Boutaleb Joutei, Moussa El Jarroudi, Fouad Mokrini, Rachid Lahlali
Journal of Natural Pesticide Research.2024; 7: 100065. CrossRef
Effects of TmTak1 silencing on AMP production as an Imd pathway component in Tenebrio molitor
Su Hyeon Hwang, Ho Am Jang, Maryam Ali Mohammadie Kojour, Keunho Yun, Yong Seok Lee, Yeon Soo Han, Yong Hun Jo
Scientific Reports.2023;[Epub] CrossRef
Assessment of biocontrol potential of different Steinernema species and their bacterial symbionts, Xenorhabdus species against larvae of almond moth, Ephestia cautella (Walker)
Ebubekir Yüksel, Nesrin Ormanoğlu, Mustafa İmren, Ramazan Canhilal
Journal of Stored Products Research.2023; 101: 102082. CrossRef
Association of Midgut Bacteria and Their Metabolic Pathways with Zika Infection and Insecticide Resistance in Colombian Aedes aegypti Populations
Andrea Arévalo-Cortés, Ashish Damania, Yurany Granada, Sara Zuluaga, Rojelio Mejia, Omar Triana-Chavez
Viruses.2022; 14(10): 2197. CrossRef
The Entomopathogenic Nematodes H. bacteriophora and S. carpocapsae Inhibit the Activation of proPO System of the Nipa Palm Hispid Octodonta nipae (Coleoptera: Chrysomelidae)
Nafiu Sanda, Bofeng Hou, Youming Hou
Life.2022; 12(7): 1019. CrossRef
Larvicidal activity of Photorhabdus and Xenorhabdus bacteria isolated from insect parasitic nematodes against Aedes aegypti and Aedes albopictus
Chanakan Subkrasae, Jiranun Ardpairin, Abdulhakam Dumidae, Pichamon Janthu, Paramaporn Muangpat, Raxsina Polseela, Sarunporn Tandhavanant, Aunchalee Thanwisai, Apichat Vitta
Acta Tropica.2022; 235: 106668. CrossRef
Entomopathogenic nematodes as potential and effective biocontrol agents against cutworms, Agrotis spp.: present and future scenario
Deepak Kumar, Pritam Kumari, Rubal Kamboj, Anil Kumar, Prakash Banakar, Vinod Kumar
Egyptian Journal of Biological Pest Control.2022;[Epub] CrossRef
Integrated Biological Control Using a Mixture of Two Entomopathogenic Bacteria, Bacillus thuringiensis and Xenorhabdus hominickii, against Spodoptera exigua and Other Congeners
Md Tafim Hossain Hrithik, Youngjin Park, Hyemi Park, Yonggyun Kim
Insects.2022; 13(10): 860. CrossRef
Photorhabdus and Xenorhabdus as Symbiotic Bacteria for Bio-Control Housefly (Musca domestica L.)
Ahmed Mohamed Abd El-Rah, Amr Mohamed Abdelazeem, Hanaa Elbrense, Santiago Vergara-Pi
Pakistan Journal of Biological Sciences.2022; 25(7): 586. CrossRef
Control Potentials of Three Entomopathogenic Bacterial Isolates for the Carob Moth, Ectomyelois ceratoniae (Lepidoptera: Pyralidae) in Pomegranates
Saqer S. Alotaibi, Hadeer Darwish, Sarah Alharthi, Akram Alghamdi, Ahmed Noureldeen, Ahmed M. Fallatah, András Fodor, Amal Al-Barty, Bander Albogami, Alaa Baazeem
Agriculture.2021; 11(12): 1256. CrossRef
Functional interaction of bacterial virulence factors of Xenorhabdus nematophila with a calcium-independent cytosolic PLA2 of Spodoptera exigua
Vatanparast Mohammad, Yonggyun Kim
Journal of Invertebrate Pathology.2020; 169: 107309. CrossRef
Immunosuppressive Activities of Novel PLA2 Inhibitors from Xenorhabdus hominickii, an Entomopathogenic Bacterium
Md. Mahi Imam Mollah, Aman Dekebo, Yonggyun Kim
Insects.2020; 11(8): 505. CrossRef
The great potential of entomopathogenic bacteria Xenorhabdus and Photorhabdus for mosquito control: a review
Wellington Junior da Silva, Harry Luiz Pilz-Júnior, Ralf Heermann, Onilda Santos da Silva
Parasites & Vectors.2020;[Epub] CrossRef
Benzylideneacetone and other phenylethylamide bacterial metabolites induce apoptosis to kill insects
Md. Mahi Imam Mollah, Fatema Yeasmin, Yonggyun Kim
Journal of Asia-Pacific Entomology.2020; 23(2): 449. CrossRef
Partner-specific induction of Spodoptera frugiperda immune genes in response to the entomopathogenic nematobacterial complex Steinernema carpocapsae-Xenorhabdus nematophila
Louise Huot, Audrey Bigourdan, Sylvie Pagès, Jean-Claude Ogier, Pierre-Alain Girard, Nicolas Nègre, Bernard Duvic
Developmental & Comparative Immunology.2020; 108: 103676. CrossRef
The prophenoloxidase system in Drosophila participates in the anti-nematode immune response
Dustin Cooper, Caitlin Wuebbolt, Christa Heryanto, Ioannis Eleftherianos
Molecular Immunology.2019; 109: 88. CrossRef
Variation in pathogenicity of different strains of Xenorhabdus nematophila; Differential immunosuppressive activities and secondary metabolite production
Md. Ariful Hasan, Shabbir Ahmed, Md. Mahi Imam Mollah, Dongwoon Lee, Yonggyun Kim
Journal of Invertebrate Pathology.2019; 166: 107221. CrossRef
Prostaglandins and Other Eicosanoids in Insects: Biosynthesis and Biological Actions
David Stanley, Yonggyun Kim
Frontiers in Physiology.2019;[Epub] CrossRef
Identification of entomopathogenic nematodes and symbiotic bacteria from Nam Nao National Park in Thailand and larvicidal activity of symbiotic bacteria against Aedes aegypti and Aedes albopictus
Temsiri Yooyangket, Paramaporn Muangpat, Raxsina Polseela, Sarunporn Tandhavanant, Aunchalee Thanwisai, Apichat Vitta, Guido Favia
PLOS ONE.2018; 13(4): e0195681. CrossRef
Differential immunosuppression by inhibiting PLA2 affects virulence of Xenorhabdus hominickii and Photorhabdus temperata temperata
Shabbir Ahmed, Yonggyun Kim
Journal of Invertebrate Pathology.2018; 157: 136. CrossRef
Challenging the larvae of Helicoverpa armigera and assessing the immune responses to nematode-bacterium complex
Istkhar, Ashok Kumar Chaubey
Phytoparasitica.2018; 46(1): 75. CrossRef
Refining the Natural Product Repertoire in Entomopathogenic Bacteria
Nicholas Jay Tobias, Yi-Ming Shi, Helge B. Bode
Trends in Microbiology.2018; 26(10): 833. CrossRef
Role of Endosymbionts in Insect–Parasitic Nematode Interactions
Ioannis Eleftherianos, Shruti Yadav, Eric Kenney, Dustin Cooper, Yaprak Ozakman, Jelena Patrnogic
Trends in Parasitology.2018; 34(5): 430. CrossRef
Eicosanoid mediation of immune responses at early bacterial infection stage and its inhibition by Photorhabdus temperata subsp. temperata, an entomopathogenic bacterium
Hyoil Kim, Duyeol Choi, Jihyeon Jung, Yonggyun Kim
Archives of Insect Biochemistry and Physiology.2018;[Epub] CrossRef
Identification and bacterial characteristics of Xenorhabdus hominickii ANU101 from an entomopathogenic nematode, Steinernema monticolum
Youngjin Park, Sangjin Kang, Md. Sadekuzzaman, Hyeonghwan Kim, Jin-Kyo Jung, Yonggyun Kim
Journal of Invertebrate Pathology.2017; 144: 74. CrossRef
The Global Transcription Factor Lrp Is both Essential for and Inhibitory to Xenorhabdus nematophila Insecticidal Activity
Ángel M. Casanova-Torres, Upasana Shokal, Neta Morag, Ioannis Eleftherianos, Heidi Goodrich-Blair, Harold L. Drake
Applied and Environmental Microbiology.2017;[Epub] CrossRef
Effects of an entomopathogen nematode on the immune response of the insect pest red palm weevil: Focus on the host antimicrobial response
Simona Binda-Rossetti, Maristella Mastore, Marina Protasoni, Maurizio F. Brivio
Journal of Invertebrate Pathology.2016; 133: 110. CrossRef
A Mixture ofBacillus thuringiensissubsp.israelensisWithXenorhabdus nematophila-Cultured Broth Enhances Toxicity Against MosquitoesAedes albopictusandCulex pipiens pallens(Diptera: Culicidae)
Youngjin Park, Jin Kyo Jung, Yonggyun Kim
Journal of Economic Entomology.2016; 109(3): 1086. CrossRef
Microbiology of sugar‐rich environments: diversity, ecology and system constraints
Bart Lievens, John E. Hallsworth, Maria I. Pozo, Zouhaier Ben Belgacem, Andrew Stevenson, Kris A. Willems, Hans Jacquemyn
Environmental Microbiology.2015; 17(2): 278. CrossRef
Comparison of Xenorhabdus bovienii bacterial strain genomes reveals diversity in symbiotic functions
Kristen E. Murfin, Amy C. Whooley, Jonathan L. Klassen, Heidi Goodrich-Blair
BMC Genomics.2015;[Epub] CrossRef
Entomopathogenic bacterium, Xenorhabdus nematophila and Photorhabdus luminescens, enhances Bacillus thuringiensis Cry4Ba toxicity against yellow fever mosquito, Aedes aegypti (Diptera: Culicidae)
Youngjin Park
Journal of Asia-Pacific Entomology.2015; 18(3): 459. CrossRef
A Technique to Enhance Insecticidal Efficacy Using Bt Cry Toxin Mixture and Eicosanoid Biosynthesis Inhibitor
Seonghyeon Eom, Youngjin Park, Yonggyun Kim
The Korean Journal of Pesticide Science.2015; 19(3): 301. CrossRef
Anopheles gambiae eicosanoids modulate Plasmodium berghei survival from oocyst to salivary gland invasion
Susana Ramos, Ana Custódio, Henrique Silveira
Memórias do Instituto Oswaldo Cruz.2014; 109(5): 668. CrossRef
Integrin β subunit and its RNA interference in immune and developmental processes of the Oriental tobacco budworm, Helicoverpa assulta
Youngjin Park, Seung-Joon Ahn, Heiko Vogel, Yonggyun Kim
Developmental & Comparative Immunology.2014; 47(1): 59. CrossRef
A Technique to Enhance Bacillus thuringiensis Spectrum and Control Efficacy Using Cry Toxin Mixture and Immunosuppressant
Seonghyeon Eom, Youngjin Park, Yonggyun Kim
The Korean Journal of Pesticide Science.2014; 18(3): 181. CrossRef
Effect of Cellular Phospholipase A2Inhibition on Enhancement of Bt Insecticidal Activity
Seonghyeon Eom, Jiyeong Park, Kunwoo Kim, Yonggyun Kim
Korean journal of applied entomology.2014; 53(3): 271. CrossRef

Review

MINIREVIEW] Development of Diagnostic and Vaccine Markers Through Cloning, Expression, and Regulation of Putative Virulence-Protein-Encoding Genes of Aeromonas hydrophila: Vijai Singh , Dharmendra Kumar Chaudhary , Indra Mani , Rohan Jain , B.N. Mishra; J. Microbiol. 2013;51(3):275-282. Published online June 28, 2013; DOI: https://doi.org/10.1007/s12275-013-2437-x

38 View
0 Download
24 Scopus

Abstract: Aeromonas hydrophila is an opportunistic bacterial pathogen that is associated with a number of diseases in fish, amphibians, reptiles, and humans. In fish it causes several disease symptoms including tail and skin rot, and haemorrhagic septicemia; in human it causes soft-tissue wound infection and diarrhoea. The pathogenesis of A. hydrophila is multifactorial, but the mechanism is unknown so far. It is considered to be mediated by expression and secretion of extracellular proteins such as aerolysin, lipase, chitinase, amylase, gelatinase, hemolysins, and enterotoxins. A number of the putative virulence-protein-encoding genes that are present in the genome of A. hydrophila have been targeted by PCR for molecular diagnosis. These significant genes are also targeted for over-production of proteins by cloning and expression methods. In this review, we emphasize recent progress in the cloning, expression, and regulation of putative virulence-protein-encoding genes of A. hydrophila for a better understanding of the pathogenesis and also help to provide effective strategies for control of diseases.

Research Support, Non-U.S. Gov'ts

Phospholipase A2 Inhibitors in Bacterial Culture Broth Enhance Pathogenicity of a Fungus Nomuraea rileyi: Jung-A Park , Yonggyun Kim; J. Microbiol. 2012;50(4):644-651. Published online July 21, 2012; DOI: https://doi.org/10.1007/s12275-012-2108-3

46 View
0 Download
10 Scopus

Abstract: An entomopathogenic fungus, Nomuraea rileyi, was isolated and its identity was confirmed by its internal transcribed spacer DNA sequence. The isolated N. rileyi exhibited a specific pathogenicity to lepidopteran species. This study was focused on enhancing the fungal pathogenicity by using immunosuppressive agents. In response to infection of N. rileyi, Spodoptera exigua larvae significantly induced catalytic activity of phospholipase A2 (PLA2) in three immune-associated tissues, namely hemocytes, fat body, and hemolymph plasma. Furthermore, the infected S. exigua larvae induced transcription of several antimicrobial peptide (AMP) genes. Two entomopathogenic bacteria, Xenorhabdus nematophila (Xn) and Photorhabdus temperata subsp. temperata (Ptt), possessed specific PLA2-inhibitory activities and their culture broths significantly inhibited the enzyme activities in hemocytes, fat body, and plasma of S. exigua. In addition, the bacterial metabolites inhibited transcription of AMP genes in S. exigua that would normally respond to the immune challenge by N. rileyi. The immunosuppressive effect of Xn or Ptt bacterial broth resulted in significant enhancement of the fungal pathogenicity against late instar larvae of S. exigua and Plutella xylostella. The effect of such a mixture was confirmed by field assay against two lepidopteran species. These results suggest that the bacterial and fungal mixture can be applied to develop a novel biopesticide to control lepidopteran species.

Immune Response Induced by ppGpp-Defective Salmonella enterica serovar Gallinarum in Chickens: Sang-Ik Park , Jae-Ho Jeong , Hyon E. Choy , Joon Haeng Rhee , Hee-Sam Na , Tae-Hoon Lee , Moon Her , Kyoung-Oh Cho , Yeongjin Hong; J. Microbiol. 2010;48(5):674-681. Published online November 3, 2010; DOI: https://doi.org/10.1007/s12275-010-0179-6

28 View
0 Download
17 Scopus

Abstract: To protect chickens from typhoid caused by Salmonella enterica serovar Gallinarum (S. Gallinarum), the attenuated 9R strain has been used in the field as a vaccine. However, safety concerns have been raised because the mutations in 9R are undefined while its efficacy is still a question under debate. A global regulator, ppGpp, synthesized by RelA and SpoT, has been shown to induce various virulence genes in S. Gallinarum (Jeong et al., 2008). In this study, two mutant strains defective in ppGpp-synthesis were constructed in wild-type S. Gallinarum (∆ppGpp) and 9R strain (9R-∆ppGpp) backgrounds and tested as live vaccines in chickens. After oral inoculation, the LD50 values of ∆ppGpp and 9R-∆ppGpp were approximately 5×1010 colony forming unit (CFU) similarly as 9R strain, which was ~105-fold higher than that of the wildtype S. Gallinarum strain. Immunological analyses revealed immunization with either of the two attenuated ppGpp-defective strains induced significant antibody responses, the production of antibody-secreting B cells in blood, proliferation of CD4+ and CD8+ T cells in the spleen, and splenic expression of proinflammatory cytokines, such as IFN-γ and TGF-β4, at levels comparable to the 9R strain. Chickens immunized with the mutants (1×108 CFU) were 80% protected against oral challenge with 1×109 wild-type virulent bacteria (4,000-fold LD50 dose), similar to the level of protection achieved by 9R immunization. Based on these data, live attenuated ∆ppGpp-defective strains may serve as novel vaccines to control fowl typhoid in chickens.

Genomic Diversity of Legionella pneumophila Serogroup 1 from Environmental Water Sources and Clinical Specimens Using Pulsed-Field Gel Electrophoresis (PFGE) from 1985 to 2007, Korea: Hae Kyung Lee , Yeon Ho Kang , Jae Yon Yu; J. Microbiol. 2010;48(5):547-553. Published online November 3, 2010; DOI: https://doi.org/10.1007/s12275-010-0031-z

37 View
0 Download
3 Scopus

Abstract: The molecular typing of 202 Legionella pneumophila sg 1 isolates obtained from environmental water sources and clinical specimens from 1985 to 2007 was conducted using pulsed-field gel electrophoresis (PFGE). In this study, a total of 212 isolates were grouped into 35 different PFGE types and Type 1 was the predominant type, accounting for 28.7% in PFGE types. Type 1 and Type 8 were observed continuously from 1985 to 2007. In the analysis of the distribution of PFGE types in six geographic regions (Seoul-Incheon, Gangwon, Chungcheong, Gyeongsang, Jeolla, and Jeju), Type 1 was predominant throughout four regions except for Jeolla and Jeju, and Type 6 was observed in four regions except two regions (Gangwon and Jeju). Six clinical isolates belonged to PFGE Type 1, Type 6, Type 9, and Type 15. Type 1 among these types, was isolated from 3 patients with confirmed nosocomial infection at the hospital and Type 6, Type 9, and Type 15 were isolated 3 patients with suspected community-acquired infection. Type R, PFGE pattern of L. pneumophila sg 1 (ATCC 33152, Philadelphia-1), was not observed in the isolates evaluated in this study. Therefore, our results suggest that PFGE Type 1 was very prevalent in the environmental and clinical isolates in Korea. Type 1 was distributed continuously for many years throughout Korea.

Biochemical Characteristics of Immune-Associated Phospholipase A2 and Its Inhibition by an Entomopathogenic Bacterium, Xenorhabdus nematophila: Sony Shrestha , Yonggyun Kim; J. Microbiol. 2009;47(6):774-782. Published online February 4, 2010; DOI: https://doi.org/10.1007/s12275-009-0145-3

26 View
0 Download
29 Scopus

Abstract: An entomopathogenic bacterium, Xenorhabdus nematophila, induces an immunosuppression of target insects by inhibiting phospholipase A2 (PLA2) activity. Recently, an immune-associated PLA2 gene was identified from the red flour beetle, Tribolium castaneum. This study cloned this PLA2 gene in a bacterial expression vector to produce a recombinant enzyme. The recombinant T. castaneum PLA2 (TcPLA2) exhibited its characteristic enzyme activity with substrate concentration, pH, and ambient temperature. Its biochemical characteristics matched to a secretory type of PLA2 (sPLA2) because its activity was inhibited by dithiothreitol (a reducing agent of disulfide bond) and bromophenacyl bromide (a specific sPLA2 inhibitor) but not by methylarachidonyl fluorophosphonate (a specific cytosolic type of PLA2). The X. nematophila culture broth contained PLA2 inhibitory factor(s), which was most abundant in the media obtained at a stationary bacterial growth phase. The PLA2 inhibitory factor(s) was heat-resistant and extracted in both aqueous and organic fractions. Effect of a PLA2-inhibitory fraction on the immunosuppression of T. castaneum was equally comparable with that resulted from inhibition of the TcPLA2 gene expression by RNA interference.

Dark Septate Endophyte (DSE) Fungi Isolated from Metal Polluted Soils: Their Taxonomic Position, Tolerance, and Accumulation of Heavy Metals In Vitro: Yujie Zhang , Yan Zhang , Maojun Liu , Xiaodong Shi , Zhiwei Zhao; J. Microbiol. 2008;46(6):624-632. Published online December 24, 2008; DOI: https://doi.org/10.1007/s12275-008-0163-6

38 View
0 Download
84 Scopus

Abstract: To understand the possible role of the plant root associated fungi on metal tolerance, their role in the uptake of heavy metals and the potential transfer of these metal ions to the plant, three strains of dark septate endophytic (DSE) fungi were isolated from a waste smelter site in southwest China, and one strain was isolated from a non-contaminated site. According to molecular phylogenetic analysis of the ITS 1-5.8S rDNA-ITS 2 gene regions and morphological characteristics, one is identified as Exophiala pisciphila, and the other three are non-sporulating fungi under the experiment condition with the nearest phylogenetic affinities to the Thysanorea papuana strain EU041814. Tolerance and accumulation abilities of the three DSE strains for metals were investigated in liquid culture. Minimum inhibitory concentrations (MIC) of Pb, Zn, and Cd were determined. It was demonstrated that the tolerance of the DSE strains varied between metal species and strains. The E. pisciphila strain is able to accumulate lead and cadmium over 20% and 5% of dry weight of biomass, respectively. Partial of the sequestrated metals can be washed with CaCl2. Morphological and enzyme activity changes taking place in the presence of excessive Pb, Cd, and/or Zn also indicate that the mechanism of heavy metal tolerance and accumulation of the DSE strains would be a complex process. The findings indicated promising tolerance and accumulation of the DSE strains with potential values in metal cycling and restoration of soil and water system.

Use of Clostridium septicum Alpha Toxins for Isolation of Various Glycosylphosphatidylinositol-Deficient Cells: Dong-Jun Shin , Hyon E. Choy , Yeongjin Hong; J. Microbiol. 2005;43(3):266-271.; DOI: https://doi.org/2214 [pii]

43 View
0 Download

Abstract: In eukaryotic cells, various proteins are anchored to the plasma membrane through glycosylphosphatidylinositol (GPI). To study the biosynthetic pathways and modifications of GPI, various mutant cells have been isolated from the cells of Chinese hamster ovaries (CHO) supplemented with several exogenous genes involved in GPI biosynthesis using aerolysin, a toxin secreted from gram-negative bacterium Aeromonas hydrophila. Alpha toxin from Gram-positive bacterium Clostridium septicum is homologous to large lobes (LL) of aerolysin, binds GPI-anchored proteins and possesses a cell-destroying mechanism similar to aerolysin. Here, to determine whether alpha toxins can be used as an isolation tool of GPI-mutants, like aerolysin, CHO cells stably transfected with several exogenous genes involved in GPI biosynthesis were chemically mutagenized and cultured in a medium containing alpha toxins. We isolated six mutants highly resistant to alpha toxins and deficient in GPI biosynthesis. By genetic complementation, we determined that one mutant cell was defective of the second subunit of dolichol phosphate mannose synthase (DPM2) and other five cells were of a putative catalytic subunit of inositol acyltransferase (PIG-W). Therefore, C. septicum alpha toxins are a useful screening probe for the isolation of various GPI-mutant cells.

Journal Article

Comparative Analysis of Intracellular Trans-Splicing Ribozyme Activity Against Hepatitis C Virus Internal Ribosome Entry Site: Kyung-Ju Ryu Seong-Wook Lee; J. Microbiol. 2004;42(4):361-364.; DOI: https://doi.org/2097 [pii]

28 View
0 Download

Abstract: Internal ribosome entry site (IRES) of the hepatitis C virus (HCV) is known to be essential for HCV replication and most conserved among HCV variants. Hence, IRES RNA is a good therapeutic target for RNA-based inhibitors, such as ribozymes. We previously proposed a new anti-HCV modulation strategy based on trans-splicing ribozymes, which can selectively replace HCV transcripts with a new RNA that exerts anti-HCV activity. To explore this procedure, sites which are accessible to ribozymes in HCV IRES were previously determined by employing an RNA mapping method in vitro. In this study, we evaluate the intracellular accessibility of the ribozymes by comparing the trans-splicing activities in cells of several ribozymes targeting different sites of the HCV IRES RNA. We assessed the intracellular activities of the ribozymes by monitoring their target-specific induction degree of both reporter gene activity and cytotoxin expression. The ribozyme capable of targeting the most accessible site identified by the mapping studies then harbored the most active trans-splicing activity in cells. These results suggest that the target sites predicted to be accessible are truly the most accessible in the cells, and thus, could be applied to the development of various RNA-based anti-HCV therapies.

Methods of the extraction of DNA from water samples for polymerase chain reaction: Jung, Hae Sung , Lee, Young Jong; J. Microbiol. 1997;35(4):354-359.

30 View
0 Download

Abstract: Methods for the extraction of DNA from water sample were approximated. Four different procedures of DNA extraction were carried out with pellets obtained from centrifugation of 4 liter water samples. The recovery efficiency and purity of DNA extracted by each method from different sources were compared. DNA yield varied with extraction methods, Method I, which involves enzymatic and freeze-thaw lysis steps and phenol and phenol-chloroform purification of extracted nucleic acid, showed a significantly higher yield and purity than the other methods. The use of glass beads in the DNA extraction methods improved the purity of DNA suitable for PCR. Bovine serum albumin in the PCR reaction mixture was useful in reducing inhibitory effects of contaminants. The efficiency of an extraction method was determined by the detection of the aer of Aeromonas hydrophila with PCR. The lower limit of detection of A. hydrophila from seeded tap water was 2 CFU/ml in PCR when method I was used for DNA preparation.

Cloning and Sequencing of the rph Gene Encoding RNase PH from Legionella pneumophila: Se Jin Kim , Jong-Seok Lim , Nicholas P. Cianciotto , Yong-Kyung Choe; J. Microbiol. 1999;37(4):218-223.

35 View
0 Download

Abstract: Legionella pneumophila, the cause of Legionnaires disease, is able to survive intracellularily in eukaryotic cells such as monocytes, macorphages, and protozoan ogranisms. During protein biosynthesis, the rph gene encodes ribonuclease (RNase) PH which functions as a phosphorolytic nuclease that removes nucleotides following the CCA terminus of tRNA and as a nucleotidyl-transferase which adds nucleotides to the ends of RNA molecules by usingnucelside diohosphates as substrates.In this sutdy, the rph gene was screened in pUC19 library employing a DNA probe whcich was constructed from PCR based on a consensus pattern of multiple alignment of RNas PH. The encoded protein consists of 235 amino acid residues with a calculated molecualr weight of 26,112 Daltons. The RNase PH signature domains are completely conserved.

Characterization of Aeromonas hydrophila Isolated from Rainbow Trouts in Korea: Soondeuk Lee , Sookyung Kim , Yoojung Oh , Yeonhee Lee; J. Microbiol. 2000;38(1):1-7.

41 View
0 Download

Abstract: Eight strains of Aeromonas hydrophila isolated from diseased trout in Korea were characterized and compared with an American type strain by various methods including biochemical and physiological tests, PCR, randomly amplified polymorphic DNA (RAPD), plasmid profiling, and gel electrophoresis of total, membrane, and extracellular proteins. Virulence factors such as surface array proteins, cytotoxin, hemolysin, haemagglutinin, and protease were also investigated. The Korean strains showed het-erogeneity in lysine decarboxylase production, utilization of various carbon sources, and production of acetoin. Five strains had the same profiles of total and membrane proteins. Six strains haemag-glutinated with trout red blood cells (RBCs) which was inhibited by fucose, galactose, and mannose, except for No. 1 where haemagglutination was inhibited by only galactose and mannose, but not by fucose. Four isolates haemagglutinated with human RBCs which was inhibited by fucose and mannose yet not by galactose. The type strain haemagglutinated only with trout RBCs which was inhibited by fucose, galactose, and mannose. Every isolate secreted protease, hemolysin, cytotoxin, and siderophore, but no enterotoxin. Results showed that the Korean isolates, except for No. 7, had very different biochemical and molecular characteristics from those of the American type strain.

First
Prev
Page of 2
Next
Last

Search