Journal of Microbiology

Journal Articles

Biosynthesis of Chryseno[2,1,c]oxepin‑12‑Carboxylic Acid from Glycyrrhizic Acid in Aspergillus terreus TMZ05‑2, and Analysis of Its Anti‑inflammatory Activity: Liangliang Chen , Lin Zhao , Ju Han , Ping Xiao , Mingzhe Zhao , Sen Zhang , Jinao Duan; J. Microbiol. 2024;62(2):113-124. Published online February 27, 2024; DOI: https://doi.org/10.1007/s12275-024-00105-4

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Glycyrrhizic acid, glycyrrhetinic acid, and their oxo, ester, lactone, and other derivatives, are known for their anti-inflammatory, anti-oxidant, and hypoglycemic pharmacological activities. In this study, chryseno[2,1-c]oxepin-12-carboxylic acid (MG) was first biosynthesized from glycyrrhizic acid through sequential hydrolysis, oxidation, and esterification using Aspergillus terreus TMZ05-2, providing a novel in vitro biosynthetic pathway for glycyrrhizic acid derivatives. Assessing the influence of fermentation conditions and variation of strains during culture under stress-induction strategies enhanced the final molar yield to 88.3% (5 g/L glycyrrhizic acid). CCK8 assays showed no cytotoxicity and good cell proliferation, and anti-inflammatory experiments demonstrated strong inhibition of NO release (36.3%, low-dose MG vs. model), transcriptional downregulation of classical effective cellular factors tumor necrosis factor-α (TNF-α; 72.2%, low-dose MG vs. model), interleukin-6 (IL-6; 58.3%, low-dose MG vs. model) and interleukin-1β (IL-1β; 76.4%, low-dose MG vs. model), and decreased abundance of P-IKK-α, P-IKB-α, and P-P65 proteins, thereby alleviating inflammatory responses through the NF-κB pathway in LPS-induced RAW264.7 cells. The findings provide a reference for the biosynthesis of lactone compounds from medicinal plants.

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Effect of different crosslinking agents on carboxymethyl chitosan-glycyrrhizic acid hydrogel: Characterization and biological activities comparison
Yinbing Wu, Zimin Gu, Tingting Chen, Duntao Zu, Yuhui Gan, Honglin Chen, Jianni Yang, Xin Yu, Huaihong Cai, Pinghua Sun, Jianying Ning, Haibo Zhou, Junxia Zheng
International Journal of Biological Macromolecules.2025; 298: 139977. CrossRef
New oxepin and dihydrobenzofuran derivatives from Bauhinia saccocalyx roots and their anti-inflammatory, cytotoxic, and antioxidant activities
Lueacha Tabtimmai, Thanyathon Phonchan, Natrinee Thongprik, Sutin Kaennakam, Nuttapon Yodsin, Kiattawee Choowongkomon, Chanikan Sonklin, Supachai Jadsadajerm, Awat Wisetsai
Journal of Natural Medicines.2025;[Epub] CrossRef
Efficient directional biosynthesis of isoquercitrin from quercetin by Bacillus subtilis CD-2 and its anti-inflammatory activity
Ju Han, Jingru Ma, Ruiqi He, Fan Yang, Jingyi Meng, Jiaqi Liu, Fanxing Shi, Jinao Duan, Liangliang Chen, Sen Zhang
Natural Product Research.2024; : 1. CrossRef

Those Nematode‑Trapping Fungi That are not Everywhere: Hints Towards Soil Microbial Biogeography: Wei Deng , Fa Zhang , Davide Fornacca , Xiao-Yan Yang , Wen Xiao; J. Microbiol. 2023;61(5):511-523. Published online April 6, 2023; DOI: https://doi.org/10.1007/s12275-023-00043-7

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Abstract

The existence of biogeography for microorganisms is a raising topic in ecology and researchers are employing better distinctions between single species, including the most rare ones, to reveal potential hidden patterns. An important volume of evidence supporting heterogeneous distributions for bacteria, archaea and protists is accumulating, and more recently a few efforts have targeted microscopic fungi. We propose an insight into this latter kingdom by looking at a group of soil nematode-trapping fungi whose species are well-known and easily recognizable. We chose a pure culture approach because of its reliable isolation procedures for this specific group. After morphologically and molecularly identifying all species collected from 2250 samples distributed in 228 locations across Yunnan province of China, we analyzed occurrence frequencies and mapped species, genera, and richness. Results showed an apparent cosmopolitan tendency for this group of fungi, including species richness among sites. However, only four species were widespread across the region, while nonrandom heterogeneous distributions were observed for the remaining 40 species, both in terms of statistical distribution of species richness reflected by a significant variance-to-mean ratio, as well as in terms of visually discernible spatial clusters of rare species and genera on the map. Moreover, several species were restricted to only one location, raising the question of whether endemicity exists for this microbial group. Finally, environmental heterogeneity showed a marginal contribution in explaining restricted distributions, suggesting that other factors such as geographical isolation and dispersal capabilities should be explored. These findings contribute to our understanding of the cryptic geographic distribution of microorganisms and encourage further research in this direction.

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Linking watershed formation with the phylogenetic distribution of a soil microscopic fungus in Yunnan Province, China
Davide Fornacca, Wei Deng, Yaoquan Yang, Fa Zhang, Xiaoyan Yang, Wen Xiao
BMC Microbiology.2024;[Epub] CrossRef
Analysis of Nuclear Dynamics in Nematode-Trapping Fungi Based on Fluorescent Protein Labeling
Liang Zhou, Zhiwei He, Keqin Zhang, Xin Wang
Journal of Fungi.2023; 9(12): 1183. CrossRef

Research Support, Non-U.S. Gov'ts

Multiple roles of a putative vacuolar protein sorting associated protein 74, FgVPS74, in the cereal pathogen Fusarium graminearum: Hee-Kyoung Kim , Ki Woo Kim , Sung-Hwan Yun; J. Microbiol. 2015;53(4):243-249. Published online April 8, 2015; DOI: https://doi.org/10.1007/s12275-015-5067-7

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Abstract

Fusarium graminearum, a member of the F. graminearum species complex, is a filamentous ascomycetous group that causes serious diseases in cereal crops. A screen of insertional mutants of F. graminearum, generated using a restriction enzyme-mediated integration method, identified a mutant designated R7048 showing pleiotropic phenotypes in several mycological traits. The vector insertion site in the R7048 genome was identified as the KpnI site within an ORF annotated as FGSG_06346 (designated FgVPS74), which showed similarity to vacuolar protein sorting-associated protein 74 in the baker yeast. Both targeted gene deletion and complementation analyses confirmed that FgVPS74 was involved in hyphal growth, conidiation, sexual development, mycotoxin production, and virulence towards host plants in F. graminearum. Electron microscopy analysis revealed no significant changes in morphology of the vacuole or other organelles, but a greater number of mitochondria were produced in the ΔFgVPS74 strain compared to the wild-type progenitor. Expression of a GFP-tagged FgVPS74 construct under its native promoter in the ΔFgVPS74 strain exhibited localization of GFP signal to putative vesicle structures, but not to the vacuolar membrane. Taken together, these findings demonstrated that a functional vacuolar protein-sorting pathway mediated by FgVPS74 is crucial for fungal growth and development in F. graminearum.

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Multi-omics lights on the toxicological effects of Cr contamination on Penicillium janthinellum P1
Qiuquan Chen, Binbin Chi, Huiying Chen, Xia Li, Bixia Xiong, Quan Guo, F. Song, Q. Chen, M. Anpo
E3S Web of Conferences.2024; 561: 03011. CrossRef
Expanding the Biological Role of Lipo-Chitooligosaccharides and Chitooligosaccharides in Laccaria bicolor Growth and Development
Manuel I. Villalobos Solis, Nancy L. Engle, Margaret K. Spangler, Sylvain Cottaz, Sébastien Fort, Junko Maeda, Jean-Michel Ané, Timothy J. Tschaplinski, Jesse L. Labbé, Robert L. Hettich, Paul E. Abraham, Tomás A. Rush
Frontiers in Fungal Biology.2022;[Epub] CrossRef
Comparative proteomics analyses of mycelial, conidial, and Secreted Proteins of high-pathogenic and weak-pathogenic Fusarium oxysporum f. sp. cucumerinum isolates
Eman Elagamey, Magdi A.E. Abdellatef, Arunima Sinha, Said M. Kamel
Physiological and Molecular Plant Pathology.2021; 115: 101675. CrossRef
A Novel DCL2-Dependent Micro-Like RNA Vm-PC-3p-92107_6 Affects Pathogenicity by Regulating the Expression of Vm-VPS10 in Valsa mali
Feiran Guo, Jiahao Liang, Ming Xu, Gao Zhang, Lili Huang, Hao Feng
Frontiers in Microbiology.2021;[Epub] CrossRef
Comparative proteomic analysis reveals molecular differences between incompatible and compatible interaction of Erysiphe pisi in garden pea
Sheetal M. Bhosle, Ragiba Makandar
Microbiological Research.2021; 248: 126736. CrossRef
Photodynamic treatment with phenothiazinium photosensitizers kills both ungerminated and germinated microconidia of the pathogenic fungi Fusarium oxysporum, Fusarium moniliforme and Fusarium solani
Henrique Dantas de Menezes, Ludmilla Tonani, Luciano Bachmann, Mark Wainwright, Gilberto Úbida Leite Braga, Marcia Regina von Zeska Kress
Journal of Photochemistry and Photobiology B: Biology.2016; 164: 1. CrossRef

Deletion analysis of LSm, FDF, and YjeF domains of Candida albicans Edc3 in hyphal growth and oxidative-stress response: Eung-Chul Kim , Jinmi Kim; J. Microbiol. 2015;53(2):111-115. Published online January 28, 2015; DOI: https://doi.org/10.1007/s12275-015-4727-y

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Abstract

Candida albicans is an opportunistic fungal pathogen whose responses to environmental changes are associated with the virulence attributes. Edc3 is known to be an enhancer of the mRNA decapping reactions and a scaffold protein of cytoplasmic processing bodies (P-bodies). Recent studies of C. albicans Edc3 suggested its critical roles in filamentous growth and stress-induced apoptotic cell death. The edc3/edc3 deletion mutant strain showed increased cell survival and less ROS accumulation upon treatment with hydrogen peroxide. To investigate the diverse involvement of Edc3 in the cellular processes, deletion mutations of LSm, FDF, or YjeF domain of Edc3 were constructed. The edc3-LSmΔ or edc3-YjeFΔ mutation showed the filamentation defect, resistance to oxidative stress, and decreased ROS accumulation. In contrast, the edc3-FDFΔ mutation exhibited a wild-type level of filamentous growth and a mild defect in ROS accumulation. These results suggest that Lsm and YjeF domains of Edc3 are critical in hyphal growth and oxidative stress response.

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The FomYjeF Protein Influences the Sporulation and Virulence of Fusarium oxysporum f. sp. momordicae
Chenxing Wei, Caiyi Wen, Yuanyuan Zhang, Hongyan Du, Rongrong Zhong, Zhengzhe Guan, Mengjiao Wang, Yanhong Qin, Fei Wang, Luyang Song, Ying Zhao
International Journal of Molecular Sciences.2023; 24(8): 7260. CrossRef
Intersection of phosphate transport, oxidative stress and TOR signalling in Candida albicans virulence
Ning-Ning Liu, Priya Uppuluri, Achille Broggi, Angelique Besold, Kicki Ryman, Hiroto Kambara, Norma Solis, Viola Lorenz, Wanjun Qi, Maikel Acosta-Zaldívar, S. Noushin Emami, Bin Bao, Dingding An, Francisco A. Bonilla, Martha Sola-Visner, Scott G. Filler,
PLOS Pathogens.2018; 14(7): e1007076. CrossRef
Mutational analysis of metacaspase CaMca1 and decapping activator Edc3 in the pathogenicity of Candida albicans
Jeong-Hoon Jeong, Seok-Eui Lee, Jinmi Kim
Fungal Genetics and Biology.2016; 97: 18. CrossRef

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