Research Support, Non-U.S. Gov't
- Alternative Mechanism for the Evaluation of Indole-3-Acetic Acid (IAA) Production by Azospirillum brasilense Strains and Its Effects on the Germination and Growth of Maize Seedlings
-
Oscar Masciarelli , Lucia Urbani , Herminda Reinoso , Virginia Luna
-
J. Microbiol. 2013;51(5):590-597. Published online September 14, 2013
-
DOI: https://doi.org/10.1007/s12275-013-3136-3
-
-
44
View
-
0
Download
-
32
Crossref
-
Abstract
-
We evaluated the production of indole-3-acetic acid (IAA) by Azospirillum brasilense strains in vitro (cell culture supernatants) and in vivo (stems and roots of maize seedlings) to clarify the role of this phytohormone as a signaling and effector molecule in the symbiotic interaction between maize and A. brasilense. The three strains all showed IAA production when cultured in NFb medium supplemented with 100 μg/ml L-tryptophan. The level of IAA production was 41.5 μg/ml for Yu62, 12.9 μg/ml for Az39, and 0.15 μg/ml for ipdC-. The release of IAA into culture medium by the bacteria appeared to be the main activator of the early growth promotion observed in the inoculated maize seedlings. The application of supernatants with different IAA contents caused significant differences in the seedling growth. This observation provides the basis for novel technological tools for effective quality control procedures on inoculants. The approach described can be incorporated into different inoculation methods, including line sowing, downspout, and foliar techniques, and increase the sustainability of symbiotic plant-bacteria systems.
-
Citations
Citations to this article as recorded by

- Enhancing Growth in Vigna radiata through the Inhibition of Charcoal Rot Disease: A Strategic Approach Using Plant Growth-Promoting Rhizobacteria
Imran Khan, Sahar Ghulam Mohyuddin, Sohail, Shah Zaman, Muhammad Qadir, Juxian Guo, Guihua Li
Microorganisms.2024; 12(9): 1852. CrossRef - Auxin-Mediated Modulation of Maize Rhizosphere Microbiome: Insights from Azospirillum Inoculation and Indole-3-Acetic Acid Treatment
Anahí Coniglio, Giovanni Larama, Sofía Nievas, Natalie L. Cale, Verónica Mora, Daniela Torres, Gastón Lopez, Florencia Donadio, Belén Rodriguez, Anelis Marin, Matias Rovere, Patricio Javier Barra, Mark F. Belmonte, Emanuel Maltempi de Souza, Fabricio Cass
Journal of Soil Science and Plant Nutrition.2024; 24(4): 6906. CrossRef - Growth and metabolism enhancement in microalgae co-cultured in suspension with the bacterium Azospirillum brasilense under heterotrophic conditions
Francisco J. Choix, Oskar A. Palacios, Claudia A. Contreras, José Carlos Espinoza-Hicks, Pedro Mondragón-Cortez, Jony R. Torres
Journal of Applied Phycology.2023; 35(1): 57. CrossRef - In Furrow Co-inoculation of Rhizobia and Azospirilla Influences the Growth and Productivity of the Common Bean
Matheus Messias, Princewill Chukwuma Asobia, Enderson Petrônio de Brito Ferreira
International Journal of Plant Production.2023; 17(4): 637. CrossRef - Effect of the Exogenous Application of Different Concentrations of Indole-3-Acetic Acid as a Growth Regulator on Onion (Allium cepa L.) Cultivation
Carlos Solano, Adriana Artola, Raquel Barrena, Cindy Ballardo, Antoni Sánchez
Agronomy.2023; 13(9): 2204. CrossRef - Pseudomonas and Curtobacterium Strains from Olive Rhizosphere Characterized and Evaluated for Plant Growth Promoting Traits
Martino Schillaci, Aida Raio, Fabiano Sillo, Elisa Zampieri, Shahid Mahmood, Muzammil Anjum, Azeem Khalid, Mauro Centritto
Plants.2022; 11(17): 2245. CrossRef - Optimization of cultural conditions using response surface methodology and modeling of indole-3-acetic acid production by Saccharothrix texasensis MB15
Abderrahmane Benadjila, Miyada Zamoum, Lamia Aouar, Abdelghani Zitouni, Yacine Goudjal
Biocatalysis and Agricultural Biotechnology.2022; 39: 102271. CrossRef - Microbiological quality analysis of inoculants based on Bradyrhizobium spp. and Azospirillum brasilense produced “on farm” reveals high contamination with non-target microorganisms
Camila Rafaeli Bocatti, Eduara Ferreira, Renan Augusto Ribeiro, Ligia Maria de Oliveira Chueire, Jakeline Renata Marçon Delamuta, Renata Katsuko Takayama Kobayashi, Mariangela Hungria, Marco Antonio Nogueira
Brazilian Journal of Microbiology.2022; 53(1): 267. CrossRef - Using plant growth-promoting microorganisms (PGPMs) to improve plant development under in vitro culture conditions
Daniel Cantabella, Ramon Dolcet-Sanjuan, Neus Teixidó
Planta.2022;[Epub] CrossRef - Synergism of Bradyrhizobium and Azospirillum baldaniorum improves growth and symbiotic performance in lima bean under salinity by positive modulations in leaf nitrogen compounds
Ágda Lorena de Oliveira Lopes, Ingrid Silva Setubal, Vicente Paulo da Costa Neto, Jerri Edson Zilli, Artenisa Cerqueira Rodrigues, Aurenivia Bonifacio
Applied Soil Ecology.2022; 180: 104603. CrossRef - Application of Azospirillum on seeds and leaves, associated with Rhizobium inoculation, increases growth and yield of common bean
Letícia Dambroz Filipini, Fernanda Kokowicz Pilatti, Edenilson Meyer, Barbara Santos Ventura, Cledimar Rogério Lourenzi, Paulo Emílio Lovato
Archives of Microbiology.2021; 203(3): 1033. CrossRef - Chickpea (Cicer arietinum L.) as model legume for decoding the co-existence of Pseudomonas fluorescens and Mesorhizobium sp. as bio-fertilizer under diverse agro-climatic zones
Sharon Nagpal, Poonam Sharma, Asmita Sirari, K.C. Kumawat, Leela Wati, S.C. Gupta, Kamalpreet Singh Mandahal
Microbiological Research.2021; 247: 126720. CrossRef - Enhancement in yield and nutritive qualities of strawberry fruits by the application of organic manures and biofertilizers
Yogesh Kumar Negi, Paramjeet Sajwan, Shweta Uniyal, A.C. Mishra
Scientia Horticulturae.2021; 283: 110038. CrossRef - Zinc solubilizing bacteria (Bacillus megaterium) with multifarious plant growth promoting activities alleviates growth in Capsicum annuum L.
Kalpana Bhatt, Dinesh Kumar Maheshwari
3 Biotech.2020;[Epub] CrossRef - What Did We Learn From Plant Growth-Promoting Rhizobacteria (PGPR)-Grass Associations Studies Through Proteomic and Metabolomic Approaches?
Dayane Alberton, Glaucio Valdameri, Vivian Rotuno Moure, Rose Adele Monteiro, Fabio de Oliveira Pedrosa, Marcelo Müller-Santos, Emanuel Maltempi de Souza
Frontiers in Sustainable Food Systems.2020;[Epub] CrossRef - Chlorothalonil tolerance of indole producing bacteria associated to wheat (Triticum turgidum L.) rhizosphere in the Yaqui Valley, Mexico
Alondra M. Díaz Rodríguez, Fannie I. Parra Cota, Gustavo Santoyo, Sergio de los Santos Villalobos
Ecotoxicology.2019; 28(5): 569. CrossRef - Decoding multifarious role of cow dung bacteria in mobilization of zinc fractions along with growth promotion of C. annuum L.
Kalpana Bhatt, Dinesh Kumar Maheshwari
Scientific Reports.2019;[Epub] CrossRef - Co-existence of Leclercia adecarboxylata (LSE-1) and Bradyrhizobium sp. (LSBR-3) in nodule niche for multifaceted effects and profitability in soybean production
K. C. Kumawat, Poonam Sharma, Inderjeet Singh, Asmita Sirari, B. S. Gill
World Journal of Microbiology and Biotechnology.2019;[Epub] CrossRef - Sulfur-oxidizing buffalo dung bacteria enhance growth and yield of Foeniculum vulgare Mill.
Sandhya Dhiman, Ramesh Chand Dubey, Dinesh Kumar Maheshwari, Sandeep Kumar
Canadian Journal of Microbiology.2019; 65(5): 377. CrossRef - Application of potassium-solubilising Proteus mirabilis MG738216 inhabiting cattle dung in improving nutrient use efficiency of Foeniculum vulgare Mill.
Sandhya Dhiman, Ramesh Chand Dubey, Nitin Baliyan, Sandeep Kumar, Dinesh Kumar Maheshwari
Environmental Sustainability.2019; 2(4): 401. CrossRef - Potential of native cold tolerant plant growth promoting bacilli to enhance nutrient use efficiency and yield of Amaranthus hypochondriacus
Chitra Pandey, Yogesh Kumar Negi, D. K. Maheshwari, Deepa Rawat, Deepti Prabha
Plant and Soil.2018; 428(1-2): 307. CrossRef - Revealing strategies of quorum sensing in Azospirillum brasilense strains Ab-V5 and Ab-V6
Josiane Fukami, Julia Laura Fernandes Abrantes, Pablo del Cerro, Marco Antonio Nogueira, Francisco Javier Ollero, Manuel Megías, Mariangela Hungria
Archives of Microbiology.2018; 200(1): 47. CrossRef - Genetic and Phenotypic Characterization of Indole-Producing Isolates of Pseudomonas syringae pv. actinidiae Obtained From Chilean Kiwifruit Orchards
Oriana Flores, Camila Prince, Mauricio Nuñez, Alejandro Vallejos, Claudia Mardones, Carolina Yañez, Ximena Besoain, Roberto Bastías
Frontiers in Microbiology.2018;[Epub] CrossRef - Co‐inoculation with Bradyrhizobium and Azospirillum Increases Yield and Quality of Soybean Seeds
Carlos Henrique Queiroz Rego, Fernanda Brito Cardoso, Ana Carina da Silva Cândido, Paulo Eduardo Teodoro, Charline Zaratin Alves
Agronomy Journal.2018; 110(6): 2302. CrossRef - MIR166a Affects the Germination of Somatic Embryos in Larixleptolepis by Modulating IAA Biosynthesis and Signaling Genes
Zhe-Xin Li, Li-Feng Zhang, Wan-Feng Li, Li-Wang Qi, Su-Ying Han
Journal of Plant Growth Regulation.2017; 36(4): 889. CrossRef - Assessment of affinity and specificity of Azospirillum for plants
Lily Pereg, Luz E. de-Bashan, Yoav Bashan
Plant and Soil.2016; 399(1-2): 389. CrossRef - Growth enhancement and drought tolerance of hybrid poplar upon inoculation with endophyte consortia
Zareen Khan, Hyungmin Rho, Andrea Firrincieli, Shang Han Hung, Virginia Luna, Oscar Masciarelli, Soo-Hyung Kim, Sharon L Doty
Current Plant Biology.2016; 6: 38. CrossRef - Morphoagronomic and productive traits of RR soybean due to inoculation via Azospirillum brasilense groove
Mario Zuffo Alan, Teodoro Bruzi Adriano, Milanez de Rezende Pedro, Cristina Bianchi Mariane, Vinicius Zambiazzi Everton, Oliveri Soares Igor, Belchior Marchetti Ribeiro Augusto, Leite Dias Vilela Guilherme
African Journal of Microbiology Research.2016; 10(13): 438. CrossRef - Foliar application of Azospirillum brasilense in soybean and seed physiological quality
Mario Zuffo Alan, Teodoro Bruzi Adriano, Milanez de Rezende Pedro, Laene Moreira de Carvalho Maria, Vinicius Zambiazzi Everton, Oliveri Soares Igor, Barroso Silva Karina
African Journal of Microbiology Research.2016; 10(20): 675. CrossRef - Composition and activity of endophytic bacterial communities in field-grown maize plants inoculated with Azospirillum brasilense
Emilyn Emy Matsumura, Vinícius Andrade Secco, Renata Stolf Moreira, Odair José Andrade Pais dos Santos, Mariangela Hungria, André Luiz Martinez de Oliveira
Annals of Microbiology.2015; 65(4): 2187. CrossRef - Indole: a signaling molecule or a mere metabolic byproduct that alters bacterial physiology at a high concentration?
Jisun Kim, Woojun Park
Journal of Microbiology.2015; 53(7): 421. CrossRef - A new PGPR co-inoculated with Bradyrhizobium japonicum enhances soybean nodulation
Oscar Masciarelli, Analía Llanes, Virginia Luna
Microbiological Research.2014; 169(7-8): 609. CrossRef