Journal of Microbiology

Research Support, Non-U.S. Gov't

Involvement of Phosphatidylinositol 3-Kinase/Akt Signaling Pathway in β1 Integrin-Mediated Internalization of Staphylococcus aureus by Alveolar Epithelial Cells: Jia-He Wang , Ke Zhang , Nan Wang , Xiao-Min Qiu , Yi-Bing Wang , Ping He; J. Microbiol. 2013;51(5):644-650. Published online June 25, 2013; DOI: https://doi.org/10.1007/s12275-013-3040-x

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The invasion of Staphylococcus aureus into alveolar epithelial cells is regarded as the key step for S. aureus lung infection. However, the mechanism of internalization of S. aureus by alveolar epithelial cells is not clear, and was the aim of this investigation Human lung adenocarcinomic epithelial cells and A549 cells were used. Human β1 integrin and rat β1 integrin were detected by real-time reverse transcription (RT)-PCR. The expressions of β1 integrin, Akt and p-Akt were detected by Western blot analysis. To further investigate the role of β1 integrin in S. aureus internalization by alveolar epithelial cells, we next performed siRNA-mediated knockdown of β1 integrin expression. In this study, we found that S. aureus invades human alveolar epithelial cells and rat primary alveolar epithelial cells. The β1 integrin ligand competitive inhibitor, GRGDS-peptide, blocked the internalization of S. aureus by A549 cells. Knockdown of β1 integrin also inhibited the internalization of S. aureus. In addition, the PI3K/Akt signaling pathway in alveolar epithelial cells was activated by the infection with S. aureus. Furthermore, Akt phosphorylation was abolished by transient transfection with β1 integrin siRNA in A549 cells challenged with S. aureus. Our results suggest that the phosphatidylinositol 3-kinase/Akt signaling pathway plays an important role in β1 integrin-mediated internalization of S. aureus by alveolar epithelial cells.

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Rapid PCR Method for Detecting Candida albicans Using Primers Derived from the Integrin-like Protein Gene [alpha]INT1 of Candida albicans: Young-Hee Lim , Do-Hyun Lee; J. Microbiol. 2000;38(2):105-108.

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Abstract: Oligonucleotide primers amplifying a 344 bp fragment on the integrin-like protein alpha-INT1p gene ([alpha]INT1) of Candida albicans were synthesized for screening of C. albicans from clinicalsamples by the polymerase chain reaction (PCR). The PCR specifically amplified DNA from C. albicans and none from any other Candida, fungal, or human DNA in standard strains used here. The PCR assay showed that the primers (LH1 and LH2) were specific for 26 isolates of C. albicans from clinical samples, whereas the positive fragment, 344 bp, was not amplified from 15 clinical isolates including 14 other medically important Candida species and an isolate of Saccharomyces cerevisiae. PCR was conducted on the urine samples of 20 patients and 4 samples were C. albicans positive. The detection limit of the PCR assay for C. albicans was shown to be approximately 10 cells/ml saline. The PCR system using 344 bp [alpha]INT1 as a target is more specific and rapid than the conventional culture method, and the sensitive detection method is applicable to clinical diagnosis of C. albicans infections.

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