MSK
Search
- Page Path
- HOME > Search
Journal Article
- CA‑CAS‑01‑A: A Permissive Cell Line for Isolation and Live Attenuated Vaccine Development Against African Swine Fever Virus
- Seung-Chul Lee , Yongkwan Kim , Ji-Won Cha , Kiramage Chathuranga , Niranjan Dodantenna , Hyeok-Il Kwon , Min Ho Kim , Weonhwa Jheong , In-Joong Yoon , Joo Young Lee , Sung-Sik Yoo , Jong-Soo Lee
- J. Microbiol. 2024;62(2):125-134. Published online March 13, 2024
- DOI: https://doi.org/10.1007/s12275-024-00116-1
- 520 View
- 5 Download
- 7 Web of Science
- 8 Crossref
-
Abstract
PDF - African swine fever virus (ASFV) is the causative agent of the highly lethal African swine fever disease that affects domestic pigs and wild boars. In spite of the rapid spread of the virus worldwide, there is no licensed vaccine available. The lack of a suitable cell line for ASFV propagation hinders the development of a safe and effective vaccine. For ASFV propagation, primary swine macrophages and monocytes have been widely studied. However, obtaining these cells can be time-consuming and expensive, making them unsuitable for mass vaccine production. The goal of this study was to validate the suitability of novel CA-CAS-01-A (CAS-01) cells, which was identified as a highly permissive cell clone for ASFV replication in the MA-104 parental cell line for live attenuated vaccine development. Through a screening experiment, maximum ASFV replication was observed in the CAS-01 cell compared to other sub-clones of MA-104 with 14.89 and log10 7.5 ± 0.15 Ct value and TCID50/ ml value respectively. When CAS-01 cells are inoculated with ASFV, replication of ASFV was confirmed by Ct value for ASFV DNA, HAD50/ ml assay, TCID50/ ml assay, and cytopathic effects and hemadsoption were observed similar to those in primary porcine alveolar macrophages after 5th passage. Additionally, we demonstrated stable replication and adaptation of ASFV over the serial passage. These results suggest that CAS-01 cells will be a valuable and promising cell line for ASFV isolation, replication, and development of live attenuated vaccines.
-
Citations
Citations to this article as recorded by
- Establishment of a highly sensitive porcine alveolar macrophage cell line for African swine fever virus
Xiangwan Lu, Xiadan Gong, Yingshuo Sun, Lang Gong, Yan Zhang
In Vitro Cellular & Developmental Biology - Animal.2025; 61(4): 425. CrossRef - Genetic and Pathogenic Characteristic of High Pathogenic Korean NADC34‐Like Porcine Reproductive and Respiratory Syndrome Virus
Sehyeong Ham, Chanhee Chae, Nan-hua Chen
Transboundary and Emerging Diseases.2025;[Epub] CrossRef - Efficient and modular reverse genetics system for rapid generation of recombinant severe acute respiratory syndrome coronavirus 2
Sojung Bae, Jinjong Myoung
Journal of Microbiology.2025; 63(7): e2504015. CrossRef - Progress in the in vitro propagation of African swine fever virus and implications for vaccine development
Thanathom Chailangkarn, Theeradej Thaweerattanasinp, Asawin Wanitchang, Janya Saenboonrueng, Challika Kaewborisuth, Anan Jongkaewwattana
Animal Diseases.2025;[Epub] CrossRef - Analysis of novel African swine fever variants circulating in wild boars in South Korea isolated in 2021 by deep sequencing
Van Dam Lai, Yong-kwan Kim, Min-Ho Kim, Yeeun Moon, Hyeok-Il Kwon, Weon-hwa Jheong, Jong-Soo Lee, Sung-Sik Yoo, In Pil Mo
Infection, Genetics and Evolution.2025; 135: 105833. CrossRef - Domain-Specific Impacts of Spike Protein Mutations on Infectivity and Antibody Escape in SARS-CoV-2 Omicron BA.1
Tae-Hun Kim, Sojung Bae, Jinjong Myoung
Journal of Microbiology and Biotechnology.2025;[Epub] CrossRef - Current status of African swine fevervaccine research and thoughts
Jiarong Yu, Shengbin Gao, Jiao Xu, Yonggang Zhao, Linlin Fang, Xiaozhen Wang, Yingli Wang, Jingyue Bao, Zhiliang Wang
Journal of Integrative Agriculture.2025;[Epub] CrossRef - Development and characterization of high-efficiency cell-adapted live attenuated vaccine candidate against African swine fever
Min Ho Kim, Ashan Subasinghe, Yongkwan Kim, Hyeok-Il Kwon, Yehjin Cho, Kiramage Chathuranga, Ji-Won Cha, Ji-Yoon Moon, Ji-Hyeon Hong, Jin Kim, Seung-Chul Lee, Niranjan Dodantenna, Nuwan Gamage, W. A. Gayan Chathuranga, Yeonji Kim, In-Joong Yoon, Joo Young
Emerging Microbes & Infections.2024;[Epub] CrossRef
- Establishment of a highly sensitive porcine alveolar macrophage cell line for African swine fever virus
First
Prev
TOP
