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- Gamma-irradiation of Streptococcus pneumoniae for the use as an immunogenic whole cell vaccine
- Min Yong Jwa , Soyoung Jeong , Eun Byeol Ko , A Reum Kim , Hyun Young Kim , Sun Kyung Kim , Ho Seong Seo , Cheol-Heui Yun , Seung Hyun Han
- J. Microbiol. 2018;56(8):579-585. Published online July 25, 2018
- DOI: https://doi.org/10.1007/s12275-018-8347-1
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Abstract
- Streptococcus pneumoniae is a major respiratory pathogen
that causes millions of deaths worldwide. Although subunit
vaccines formulated with the capsular polysaccharides or
their protein conjugates are currently-available, low-cost
vaccines with wide serotype coverage still remain to be developed,
especially for developing countries. Recently, gamma-
irradiation has been considered as an effective inactivation
method
to prepare S. pneumoniae vaccine candidate. In this study, we investigated the immunogenicity and protective immunity of gamma-irradiated S. pneumoniae (r-SP), by comparing with heat-inactivated S. pneumoniae (h-SP) and formalin-inactivated S. pneumoniae (f-SP), both of which were made by traditional inactivation methods. Intranasal immunization of C57BL/6 mice with r-SP in combination with cholera toxin as an adjuvant enhanced S. pneumoniaespecific antibodies on the airway mucosal surface and in sera more potently than that with h-SP or f-SP under the same conditions. In addition, sera from mice immunized with r- SP potently induced opsonophagocytic killing activity more effectively than those of h-SP or f-SP, implying that r-SP could induce protective antibodies. Above all, immunization with r-SP effectively protected mice against S. pneumoniae infection. Collectively, these results suggest that gamma- irradiation is an effective method for the development of a killed whole cell pneumococcal vaccine that elicits robust mucosal and systemic immune responses.
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