Journal of Microbiology

Full articles

Inhibition of cardiolipin biosynthesis partially suppresses the sensitivity of an Escherichia coli mutant lacking OmpC to envelope stress: Dae-Beom Ryu, Umji Choi, Gyubin Han, Chang-Ro Lee; J. Microbiol. 2025;63(11):e2507004. Published online November 30, 2025; DOI: https://doi.org/10.71150/jm.2507004

933 View
16 Download

Abstract PDF Supplementary Material: Porins in the outer membrane (OM) of Gram-negative bacteria play two main functions: passage of various extracellular molecules and maintenance of membrane integrity. OmpC, a non-specific porin, is involved in both functions; however, the exact mechanism of maintenance of membrane integrity remains unknown. In this study, we found that inhibiting cardiolipin biosynthesis partially restored the growth defect of the ompC mutant under envelope stress. Among the three enzymes involved in cardiolipin biosynthesis, ClsABC, this effect is primarily associated with ClsA. Notably, the deletion of ClsA also suppressed the similar phenotypes of an Escherichia coli mutant lacking YhdP, a transmembrane protein involved in phospholipid transport from the inner membrane to the OM. Collectively, these results imply that OmpC may contribute to membrane integrity, partially through mechanisms linked to transport or biosynthesis of phospholipids such as cardiolipin.

Safety evaluation and hypolipidemic ability of water-soluble blue pigment extracted by HPD-400 resin from Quambalaria cyanescens: Ruobing Shi, Chengzhong Wang, Nianping Xue, Zhiguo Zhang; J. Microbiol. 2025;63(11):e2412011. Published online November 30, 2025; DOI: https://doi.org/10.71150/jm.2412011

793 View
9 Download

Abstract PDF: The oral administration of synthetic drugs can effectively reduce blood lipid levels, but adverse reactions may occur. Because of this, the hypolipidemic ability of natural products has been increasingly investigated. We evaluate the safety and hypolipidemic characteristics of a water-soluble blue pigment extracted using HPD-400 resin from the fungus Quambalaria cyanescens. Hypolipidemic ability was examined by constructing a hyperlipidemia model with different doses of blue pigment (50, 100, and 200 mg/kg. mouse body weight) for 28 d. Blue pigment purity increased from 20.32% to 70.70% following treatment with HPD-400 resin. Acute toxicity tests revealed blue pigment sourced from Q. cyanescens to have no toxic effects on mouse body weight, mortality, or behavioral characteristics. Subacute toxicity tests revealed no significant differences in food intake, body weight, or organ weights between treatment groups and controls. Histopathological examination of the liver and kidney tissues of mice administered blue pigment were normal, and serum enzyme activities and blood constituents were also within normal ranges. Blue pigment can significantly reduce the weight of mice, reduce liver and kidney damage and fat accumulation. It can also reduce total cholesterol, triglyceride and low density lipoprotein cholesterol in serum and liver tissue, and increase the level of high density lipoprotein cholesterol. Reduce the levels of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, creatinine, urea and uric acid in serum. Increase the activities of total superoxide dismutase, glutathione peroxidase and catalase in serum and liver tissue, reduce the content of malondialdehyde, and up-regulate liver lipase and lipoprotein lipase. Our work proves that blue pigment is nontoxic, has the function of reducing blood lipid, and can alleviate obesity-related symptoms by regulating lipid metabolism and oxidative stress.

Journal Articles

Lactobacillus gasseri BNR17 and Limosilactobacillus fermentum ABF21069 Ameliorate High Sucrose-Induced Obesity and Fatty Liver via Exopolysaccharide Production and β-oxidation: Yu Mi Jo, Yoon Ji Son, Seul-Ah Kim, Gyu Min Lee, Chang Won Ahn, Han-Oh Park, Ji-Hyun Yun; J. Microbiol. 2024;62(10):907-918. Published online October 17, 2024; DOI: https://doi.org/10.1007/s12275-024-00173-6

993 View
16 Download
5 Web of Science
1 Crossref

Abstract PDF

Obesity and metabolic dysfunction-associated fatty liver disease (MAFLD) are prevalent metabolic disorders with substantial global health implications that are often inadequately addressed by current treatments and may have side effects. Probiotics have emerged as promising therapeutic agents owing to their beneficial effects on gut health and metabolism. This study investigated the synergistic effects of a probiotic combination of BNR17 and ABF21069 on obesity and MAFLD in C57BL/6 mice fed a high-sucrose diet. The probiotic combination significantly reduced body weight and fat accumulation compared with the high-sucrose diet. It also alleviated elevated serum leptin levels induced by a high-sucrose diet. Histological analysis revealed a significant reduction in white adipose tissue and fatty liver in the mice treated with the probiotic combination. Furthermore, increased expression of genes related to β-oxidation, thermogenesis, and lipolysis suggested enhanced metabolic activity. The probiotic groups, particularly the BNR17 group, showed an increase in fecal exopolysaccharides, along with a tendency toward a lower expression of intestinal sugar transport genes, indicating reduced sugar absorption. Additionally, inflammatory markers in the liver tissue exhibited lower expression in the ABF21069 group than in the HSD group. Despite each strain in the combination group having distinct characteristics and functions, their combined effect demonstrated synergy in mitigating obesity and MAFLD, likely through the modulation of fecal exopolysaccharides content and improvement in lipid metabolism. These findings underscore the potential of probiotic supplementation as a promising assistant therapy for managing obesity and MAFLD and provide valuable insights into its therapeutic mechanisms in metabolic disorders.

Citations

Citations to this article as recorded by

A unique tetrasaccharide-containing anchor glycolipid of lipoteichoic acid is commonly found in Lactobacillus gasseri and Lactobacillus paragasseri
Tsukasa Shiraishi, Ryosuke Kutomi, Yamaha Sato, Akihito Endo, Satoru Fukiya, Satoshi Takahashi, Atsushi Yokota, Shin-ichi Yokota
Bioscience, Biotechnology, and Biochemistry.2025; 89(9): 1382. CrossRef

Characterization of staphylococcal endolysin LysSAP33 possessing untypical domain composition: Jun-Hyeok Yu , Do-Won Park , Jeong-A Lim , Jong-Hyun Park; J. Microbiol. 2021;59(9):840-847. Published online August 12, 2021; DOI: https://doi.org/10.1007/s12275-021-1242-1

335 View
0 Download
8 Web of Science
7 Crossref

Abstract PDF

Endolysin, a peptidoglycan hydrolase derived from bacteriophage, has been suggested as an alternative antimicrobial agent. Many endolysins on staphylococcal phages have been identified and applied extensively against Staphylococcus spp. Among them, LysK-like endolysin, a well-studied staphylococcal endolysin, accounts for most of the identified endolysins. However, relatively little interest has been paid to LysKunlike endolysin and a few of them has been characterized. An endolysin LysSAP33 encoded on bacteriophage SAP33 shared low homology with LysK-like endolysin in sequence by 41% and domain composition (CHAP-unknown CBD). A green fluorescence assay using a fusion protein for Lys- SAP33_CBD indicated that the CBD domain (157-251 aa) was bound to the peptidoglycan of S. aureus. The deletion of LysSAP33_CBD at the C-terminal region resulted in a significant decrease in lytic activity and efficacy. Compared to LysK-like endolysin, LysSAP33 retained its lytic activity in a broader range of temperature, pH, and NaCl concentrations. In addition, it showed a higher activity against biofilms than LysK-like endolysin. This study could be a helpful tool to develop our understanding of staphylococcal endolysins not belonging to LysK-like endolysins and a potential biocontrol agent against biofilms.

Citations

Citations to this article as recorded by

Phage-Derived Endolysins Against Resistant Staphylococcus spp.: A Review of Features, Antibacterial Activities, and Recent Applications
Mina Golban, Javad Charostad, Hossein Kazemian, Hamid Heidari
Infectious Diseases and Therapy.2025; 14(1): 13. CrossRef
Characterization of a phage endolysin LysLFP01 and its antibacterial activity
Qiannan Wen, Xuecheng Huang, Wenxin Ma, Yingtong Chen, Luyao Wang, Yang Ma, Xia Chen
International Journal of Food Microbiology.2025; 432: 111110. CrossRef
ZAM-CS, a novel chimeric endolysin with enhanced stability and rapid action against methicillin-resistant Staphylococcus aureus
Yasaman Ahmadbeigi, Neda Soleimani, Farzaneh Azizmohseni, Zahra Amini-Bayat
BMC Microbiology.2025;[Epub] CrossRef
Molecular Machinery of the Triad Holin, Endolysin, and Spanin: Key Players Orchestrating Bacteriophage-Induced Cell Lysis and their Therapeutic Applications
Safia Samir
Protein & Peptide Letters.2024; 31(2): 85. CrossRef
A Novel Truncated CHAP Modular Endolysin, CHAPSAP26-161, That Lyses Staphylococcus aureus, Acinetobacter baumannii, and Clostridioides difficile, and Exhibits Therapeutic Effects in a Mouse Model of A. baumannii Infection
Yoon-Jung Choi, Shukho Kim, Ram Hari Dahal, Jungmin Kim
Journal of Microbiology and Biotechnology.2024; 34(8): 1718. CrossRef
Therapeutic potential of bacteriophage endolysins for infections caused by Gram-positive bacteria
He Liu, Zhen Hu, Mengyang Li, Yi Yang, Shuguang Lu, Xiancai Rao
Journal of Biomedical Science.2023;[Epub] CrossRef
Endolysin, a Promising Solution against Antimicrobial Resistance
Mujeeb ur Rahman, Weixiao Wang, Qingqing Sun, Junaid Ali Shah, Chao Li, Yanmei Sun, Yuanrui Li, Bailing Zhang, Wei Chen, Shiwei Wang
Antibiotics.2021; 10(11): 1277. CrossRef

The effects of deletion of cellobiohydrolase genes on carbon source-dependent growth and enzymatic lignocellulose hydrolysis in Trichoderma reesei: Meibin Ren , Yifan Wang , Guoxin Liu , Bin Zuo , Yuancheng Zhang , Yunhe Wang , Weifeng Liu , Xiangmei Liu , Yaohua Zhong; J. Microbiol. 2020;58(8):687-695. Published online June 10, 2020; DOI: https://doi.org/10.1007/s12275-020-9630-5

375 View
0 Download
13 Web of Science
11 Crossref

Abstract PDF

The saprophytic fungus Trichoderma reesei has long been used as a model to study microbial degradation of lignocellulosic biomass. The major cellulolytic enzymes of T. reesei are the cellobiohydrolases CBH1 and CBH2, which constitute more than 70% of total proteins secreted by the fungus. However, their physiological functions and effects on enzymatic hydrolysis of cellulose substrates are not sufficiently elucidated. Here, the cellobiohydrolase-encoding genes cbh1 and cbh2 were deleted, individually or combinatively, by using an auxotrophic marker-recycling technique in T. reesei. When cultured on media with different soluble carbon sources, all three deletion strains (Δcbh1, Δcbh2, and Δcbh1Δcbh2) exhibited no dramatic variation in morphological phenotypes, but their growth rates increased apparently when cultured on soluble cellulase-inducing carbon sources. In addition, Δcbh1 showed dramatically reduced growth and Δcbh1Δcbh2 could hardly grew on microcrystalline cellulose (MCC), whereas all strains grew equally on sodium carboxymethyl cellulose (CMC-Na), suggesting that the influence of the CBHs on growth was carbon source-dependent. Moreover, five representative cellulose substrates were used to analyse the influence of the absence of CBHs on saccharification efficiency. CBH1 deficiency significantly affected the enzymatic hydrolysis rates of various cellulose substrates, where acid pre-treated corn stover (PCS) was influenced the least. CBH2 deficiency reduced the hydrolysis of MCC, PCS, and acid pre-treated and delignified corncob but improved the hydrolysis ability of filter paper. These results demonstrate the specific contributions of CBHs to the hydrolysis of different types of biomass, which could facilitate the development of tailor-made strains with highly efficient hydrolysis enzymes for certain biomass types in the biofuel industry.

Citations

Citations to this article as recorded by

Functional characterization of CEL3C reveals its critical role in regulating cellulase gene expression in Trichoderma reesei Rut C30
Lu Wang, Xiao He, Tian Tian, Jian Cheng, Ruolan Cao, Jie Hou, Hui Lin, Yonghao Li
Enzyme and Microbial Technology.2025; 190: 110706. CrossRef
The Role of Non-Catalytic Region in Determining the Difference in Efficiency Between Two Cellobiohydrolases Revealed Through a Genetic Approach
Xinyuan Yan, Pankajkumar Ramdas Waghmare, Xiaoli Meng, Jianhui Zhang, Shaoming Ding, Yu Lei, Jun Yue, Guodong Liu
Journal of Fungi.2025; 11(7): 536. CrossRef
Considerations for Domestication of Novel Strains of Filamentous Fungi
Randi M. Pullen, Stephen R. Decker, Venkataramanan Subramanian, Meaghan J. Adler, Alexander V. Tobias, Matthew Perisin, Christian J. Sund, Matthew D. Servinsky, Mark T. Kozlowski
ACS Synthetic Biology.2025; 14(2): 343. CrossRef
An efficient CRISPR/Cas9 genome editing system based on a multiple sgRNA processing platform in Trichoderma reesei for strain improvement and enzyme production
Jiaxin Zhang, Kehang Li, Yu Sun, Cheng Yao, Weifeng Liu, Hong Liu, Yaohua Zhong
Biotechnology for Biofuels and Bioproducts.2024;[Epub] CrossRef
Transcriptome-wide analysis of a superior xylan degrading isolate Penicillium oxalicum 5–18 revealed active lignocellulosic degrading genes
Shuang Hu, Pei Han, Bao-Teng Wang, Long Jin, Hong-Hua Ruan, Feng-Jie Jin
Archives of Microbiology.2024;[Epub] CrossRef
Engineering the secretome of Aspergillus niger for cellooligosaccharides production from plant biomass
Fernanda Lopes de Figueiredo, Fabiano Jares Contesini, César Rafael Fanchini Terrasan, Jaqueline Aline Gerhardt, Ana Beatriz Corrêa, Everton Paschoal Antoniel, Natália Sayuri Wassano, Lucas Levassor, Sarita Cândida Rabelo, Telma Teixeira Franco, Uffe Hasb
Microbial Cell Factories.2024;[Epub] CrossRef
Constitutive overexpression of cellobiohydrolase 2 in Trichoderma reesei reveals its ability to initiate cellulose degradation
Yubo Wang, Meibin Ren, Yifan Wang, Lu Wang, Hong Liu, Mei Shi, Yaohua Zhong
Engineering Microbiology.2023; 3(1): 100059. CrossRef
Inducer-free recombinant protein production in Trichoderma reesei: secretory production of endogenous enzymes and heterologous nanobodies using glucose as the sole carbon source
Toshiharu Arai, Mayumi Wada, Hiroki Nishiguchi, Yasushi Takimura, Jun Ishii
Microbial Cell Factories.2023;[Epub] CrossRef
The Influence of Trctf1 Gene Knockout by CRISPR–Cas9 on Cellulase Synthesis by Trichoderma reesei with Various Soluble Inducers
Yudian Chen, Yushan Gao, Zancheng Wang, Nian Peng, Xiaoqin Ran, Tingting Chen, Lulu Liu, Yonghao Li
Fermentation.2023; 9(8): 746. CrossRef
The effect of cellobiohydrolase 1 gene knockout for composition and hydrolytic activity of the enzyme complex secreted by filamentous fungus Penicillium verruculosum
Valeriy Yu. Kislitsin, Andrey M. Chulkin, Ivan N. Zorov, Yuri А. Denisenko, Arkadiy P. Sinitsyn, Alexandra M. Rozhkova
Bioresource Technology Reports.2022; 18: 101023. CrossRef
Deciphering the efficient cellulose degradation by the thermophilic fungus Myceliophthora thermophila focused on the synergistic action of glycoside hydrolases and lytic polysaccharide monooxygenases
Xing Qin, Jiahuan Zou, Kun Yang, Jinyang Li, Xiaolu Wang, Tao Tu, Yuan Wang, Bin Yao, Huoqing Huang, Huiying Luo
Bioresource Technology.2022; 364: 128027. CrossRef

WasC, a WASP family protein, is involved in cell adhesion and migration through regulation of F-actin polymerization in Dictyostelium: Pyeonghwa Jeon , Taeck Joong Jeon; J. Microbiol. 2020;58(8):696-702. Published online June 10, 2020; DOI: https://doi.org/10.1007/s12275-020-0138-9

379 View
0 Download
3 Web of Science
3 Crossref

Abstract PDF

The actin cytoskeleton is involved in the regulation of cell morphology and migration. Wiskott-Aldrich Syndrome proteins (WASPs) play an important role in controlling actin polymerization by activating the Arp2/3 complex. The present study investigated the roles of WasC, one of the 3 WASPs in Dictyostelium, in cellular processes. Cells lacking WasC displayed strong cell adhesion and approximately 1.5-fold increase in F-actin levels as compared to the wild-type cells. Loss of wasC caused defects in phagocytosis and decreased the migration speed in chemoattractant-mediated cell migration but did not affect directionality. WasC was localized to the protruding region in migrating cells and, transiently and rapidly translocated to the cell cortex in response to chemoattractant stimulation, in an F-actin dependent manner. Our
results
suggest that WasC is involved in cell adhesion and migration by regulating F-actin polymerization at the leading edge of migrating cells, probably as a negative regulator. The increased strength of adhesion in wasC null cells is likely to decrease the migration speed but not the directionality.

Citations

Citations to this article as recorded by

Wiskott-Aldrich syndrome gene as a prognostic biomarker correlated with immune infiltrates in clear cell renal cell carcinoma
Guixin Ding, Tianqi Wang, Shangjing Liu, Zhongbao Zhou, Jian Ma, Jitao Wu
Frontiers in Immunology.2023;[Epub] CrossRef
Dual regulation of the actin cytoskeleton by CARMIL-GAP
Goeh Jung, Miao Pan, Christopher J. Alexander, Tian Jin, John A. Hammer
Journal of Cell Science.2022;[Epub] CrossRef
Regulation of the Actin Cytoskeleton via Rho GTPase Signalling in Dictyostelium and Mammalian Cells: A Parallel Slalom
Vedrana Filić, Lucija Mijanović, Darija Putar, Antea Talajić, Helena Ćetković, Igor Weber
Cells.2021; 10(7): 1592. CrossRef

Comparative analysis of the gut microbiota in distinct statin response patients in East China: Baoqing Sun , Luming Li , Xinfu Zhou; J. Microbiol. 2018;56(12):886-892. Published online November 27, 2018; DOI: https://doi.org/10.1007/s12275-018-8152-x

333 View
0 Download
32 Web of Science
31 Crossref

Abstract PDF

Statin response shows great interindividual variations. Recently, emerging studies have shown that gut microbiota is linked to therapeutic responses to drugs, including statins. However, the association between the gut bacteria composition and statin response is still unclear. In this study, gut microbiota of 202 hyperlipidemic patients with statin sensitive (SS) response and statin resistant (SR) response in East China were investigated by high throughput sequencing to compare the gut bacteria composition and biodiversity in distinct statin response patients. Higher biodiversity was detected in Group SS than Group SR. Specifically, group SS showed significantly increased proportion of genera Lactobacillus (P = 0.001), Eubacterium (P = 0.004), Faecalibacterium (P = 0.005), and Bifidobacterium (P = 0.002) and decreased proportion of genus Clostridium (P = 0.001) compared to Group SR. The results indicated that higher gut biodiversity was associated with statin sensitive response. The increased genera Lactobacillus, Eubacterium, Faecalibacterium, Bifidobacterium, and decreased genus Clostridium in patient gut microbiota may predict patient's statin response, and hence may guide statin dosage adjustments.

Citations

Citations to this article as recorded by

Trimethylamine N-oxide in cardiovascular disease: Pathophysiology and the potential role of statins
Fakhar Latif, Ayesha Mubbashir, Muhammad Sohaib Khan, Zain Shaikh, Aaima Memon, Jenelle Alvares, Ayesha Azhar, Hritvik Jain, Raheel Ahmed, Sai Gautham Kanagala
Life Sciences.2025; 361: 123304. CrossRef
Antibiotic-induced gut dysbiosis: unraveling the gut-heart axis and its impact on cardiovascular health
Navpreet Kaur, Pankaj Kumar, Mahadev Dhami, Khadga Raj Aran
Molecular Biology Reports.2025;[Epub] CrossRef
Lipid metabolism correction with statins and probiotics
N. V. Baryshnikova, V. M. L’Nyavina, Yu. P. Uspenskiy, A. N. Suvorov, E. I. Ermolenko
Experimental and Clinical Gastroenterology.2025; (9): 161. CrossRef
Statin therapy associated Lactobacillus intestinalis attenuates pancreatic fibrosis through remodeling intestinal homeostasis
Yuhang Sui, Tao Zhang, Suwen Ou, Guanqun Li, Liwei Liu, Tianqi Lu, Can Zhang, Yukai Cao, Rui Bai, Haoxin Zhou, Xinbo Zhao, Yue Yuan, Gang Wang, Hua Chen, Rui Kong, Bei Sun, Le Li
npj Biofilms and Microbiomes.2025;[Epub] CrossRef
Hyperlipidemia and gut microbiota: the role of prebiotics, probiotics, statins and fibrates
I. N. Grigor’eva, N. L. Tov, D. L. Nepomnyashchikh
Сибирский научный медицинский журнал.2025; 45(3): 6. CrossRef
Therapeutic potential of atorvastatin in ischemic stroke: an investigation into its anti-inflammatory effect by targeting the gut-brain axis
Liuzhu Chen, Linpei Zhuo, Jie Zheng, Xiaoyun Sun, Jing Sun, Jiaming Liu
Journal of Translational Medicine.2025;[Epub] CrossRef
Bidirectional relationship between statins and the gut microbiota: implications for cardiovascular health, diabetes, and cancer
Francisco Alejandro Lagunas-Rangel
Xenobiotica.2025; 55(7): 554. CrossRef
The Role of Gut Microbiota in the Etiopathogenesis of Multiple Chronic Diseases
Lara Pires, Ana M. González-Paramás, Sandrina A. Heleno, Ricardo C. Calhelha
Antibiotics.2024; 13(5): 392. CrossRef
Human gut microbiome: Therapeutic opportunities for metabolic syndrome—Hype or hope?
Angela Horvath, Kristina Zukauskaite, Olha Hazia, Irina Balazs, Vanessa Stadlbauer
Endocrinology, Diabetes & Metabolism.2024;[Epub] CrossRef
The Bidirectional Relationship Between Cardiovascular Medications and Oral and Gut Microbiome Health: A Comprehensive Review
Gangani Dharmarathne, Samia Kazi, Shalinie King, Thilini N. Jayasinghe
Microorganisms.2024; 12(11): 2246. CrossRef
COPD Pathogenesis and Alterations in the Oral, Lung, and Gut Microbiomes
Nobuhiro Asai, Yoshihiro Ohkuni, Hideo Kato, Mao Hagihara, Hiroshige Mikamo, Norihiro Kaneko
Microbiology Research.2024; 15(3): 1605. CrossRef
A gut feeling of statin
Jianqing She, Lizhe Sun, Yue Yu, Heze Fan, Xia Li, Xinyu Zhang, Xiaozhen Zhuo, Manyun Guo, Junhui Liu, Peining Liu, Gulinigaer Tuerhongjiang, Bin Du, Hongbing Li, Jun Yu, Zuyi Yuan, Yue Wu
Gut Microbes.2024;[Epub] CrossRef
A Systematic Review of Statins for the Treatment of Nonalcoholic Steatohepatitis: Safety, Efficacy, and Mechanism of Action
Shiqin Zhang, Xiaoling Ren, Bingzheng Zhang, Tian Lan, Bing Liu
Molecules.2024; 29(8): 1859. CrossRef
Drug-gut Microbiome Interaction in Atherosclerosis Therapeutics
Hao-Jian Zhang, Yan Wang, Jian-Dong Jiang
Current Drug Metabolism.2023; 24(7): 482. CrossRef
Treatment of Dyslipidemia through Targeted Therapy of Gut Microbiota
Brandon Flaig, Rachel Garza, Bhavdeep Singh, Sevag Hamamah, Mihai Covasa
Nutrients.2023; 15(1): 228. CrossRef
Role of Gut Microbiome in Atherosclerosis: Molecular and Therapeutic Aspects
Juan Salazar, Valery Morillo, María K Suárez, Ana Castro, Paola Ramírez, Milagros Rojas, Roberto Añez, Luis D'Marco, Maricarmen Chacín-González, Valmore Bermúdez
Current Cardiology Reviews.2023;[Epub] CrossRef
Reduced gut microbial diversity in familial hypercholesterolemia with no effect of omega-3 polyunsaturated fatty acids intervention – a pilot trial
Christopher Storm-Larsen, Liv Nesse Hande, Martin Kummen, Hilde Thunhaug, Beate Vestad, Simen Hyll Hansen, Anders Hovland, Marius Trøseid, Knut Tore Lappegård, Johannes R. Hov
Scandinavian Journal of Clinical and Laboratory Investigation.2022; 82(5): 363. CrossRef
Targets of statins intervention in LDL-C metabolism: Gut microbiota
ChangXin Sun, ZePing Wang, LanQing Hu, XiaoNan Zhang, JiYe Chen, ZongLiang Yu, LongTao Liu, Min Wu
Frontiers in Cardiovascular Medicine.2022;[Epub] CrossRef
Novel insights in the relationship of gut microbiota and coronary artery diseases
Inmaculada Ramírez-Macías, Esteban Orenes-Piñero, Anny Camelo-Castillo, José Miguel Rivera-Caravaca, Cecilia López-García, Francisco Marín
Critical Reviews in Food Science and Nutrition.2022; 62(14): 3738. CrossRef
How Brain Infarction Links With the Microbiota–Gut–Brain Axis: Hints From Studies Focusing on the Risk Factors for Ischemic Stroke
Yunpeng Liu, Jing Dong, Ziqing Zhang, Yiqi Liu, Yang Wang
Frontiers in Neuroscience.2022;[Epub] CrossRef
The promise of the gut microbiome as part of individualized treatment strategies
Daniel A. Schupack, Ruben A. T. Mars, Dayne H. Voelker, Jithma P. Abeykoon, Purna C. Kashyap
Nature Reviews Gastroenterology & Hepatology.2022; 19(1): 7. CrossRef
Human Gut Microbiota in Coronary Artery Disease: A Systematic Review and Meta-Analysis
Marcin Choroszy, Kamil Litwinowicz, Robert Bednarz, Tomasz Roleder, Amir Lerman, Takumi Toya, Karol Kamiński, Emilia Sawicka-Śmiarowska, Magdalena Niemira, Beata Sobieszczańska
Metabolites.2022; 12(12): 1165. CrossRef
The Novel Interplay between Commensal Gut Bacteria and Metabolites in Diet-Induced Hyperlipidemic Rats Treated with Simvastatin
Siruo Zhang, Lu Yuan, Huan Li, Lei Han, Wanghui Jing, Xiaokang Wu, Shakir Ullah, Ruina Liu, Yonghong Wu, Jiru Xu
Journal of Proteome Research.2022; 21(3): 808. CrossRef
A Comprehensive Analysis of Genomics and Metagenomics in a Heterozygote Familial Hypercholesterolemia Family
Honghong Liu, Ye Jin, Ran Tian, Siqin Feng, Shuyang Zhang, Chenhong Zhang
Frontiers in Cellular and Infection Microbiology.2021;[Epub] CrossRef
The gut microbiota is associated with clinical response to statin treatment in patients with coronary artery disease
Lijun Wang, Weiwei Zhou, Manyun Guo, Yiming Hua, Baihua Zhou, Xinyin Li, Xinxin Zhang, Jiakun Dong, Xiumei Yang, Yang Wang, Yue Wu, Jianqing She, Jianjun Mu
Atherosclerosis.2021; 325: 16. CrossRef
The Role of Gut Microbiota on Cholesterol Metabolism in Atherosclerosis
Margaret Vourakis, Gaétan Mayer, Guy Rousseau
International Journal of Molecular Sciences.2021; 22(15): 8074. CrossRef
Gut microbiome and cardiovascular disease
Yongzhong Zhao, Zeneng Wang
Current Opinion in Cardiology.2020; 35(3): 207. CrossRef
Gut bacterial microbiome composition and statin intake—A systematic review
Andreia M. Dias, Gonçalo Cordeiro, Maria M. Estevinho, Rui Veiga, Luis Figueira, Marta Reina‐Couto, Fernando Magro
Pharmacology Research & Perspectives.2020;[Epub] CrossRef
Unraveling Host-Gut Microbiota Dialogue and Its Impact on Cholesterol Levels
Remy Villette, Pukar KC, Sophie Beliard, Maria Fernanda Salas Tapia, Dominique Rainteau, Maryse Guerin, Philippe Lesnik
Frontiers in Pharmacology.2020;[Epub] CrossRef
Diagnostic and therapeutic potential of the gut microbiota in patients with early hepatocellular carcinoma
Francesca Romana Ponziani, Alberto Nicoletti, Antonio Gasbarrini, Maurizio Pompili
Therapeutic Advances in Medical Oncology.2019;[Epub] CrossRef
Genome analysis of Rubritalea profundi SAORIC-165T, the first deep-sea verrucomicrobial isolate, from the northwestern Pacific Ocean
Jaeho Song, Ilnam Kang, Yochan Joung, Susumu Yoshizawa, Ryo Kaneko, Kenshiro Oshima, Masahira Hattori, Koji Hamasaki, Kazuhiro Kogure, Soochan Kim, Kangseok Lee, Jang-Cheon Cho
Journal of Microbiology.2019; 57(5): 413. CrossRef

Photosynthetic and biochemical responses of the freshwater green algae Closterium ehrenbergii Meneghini (Conjugatophyceae) exposed to the metal coppers and its implication for toxicity testing: Hui Wang , Vinitha Ebenezer , Jang-Seu Ki; J. Microbiol. 2018;56(6):426-434. Published online June 1, 2018; DOI: https://doi.org/10.1007/s12275-018-8081-8

380 View
0 Download
28 Crossref

Abstract PDF

The freshwater green algae Closterium is sensitive to water quality, and hence has been suggested as ideal organisms for toxicity testing. In the present study, we evaluated the photosynthetic and biochemical responses of C. ehrenbergii to the common contaminants, coppers. The 72 h median effective concentrations (EC50) of CuSO4 and CuCl2 on the test organism were calculated to be 0.202 mg/L and 0.245 mg/L, respectively. Exposure to both coppers considerably decreased pigment levels and photosynthetic efficiency, while inducing the generation of reactive oxygen species (ROS) in cells with increased exposure time. Moreover, the coppers significantly increased the levels of lipid peroxidation and superoxide dismutase (SOD) activity, even at relatively lower concentrations. These suggest that copper contaminants may exert deleterious effects on the photosynthesis and cellular oxidative stress of C. ehrenbergii, representing its powerful potential in aquatic toxicity assessments.

Citations

Citations to this article as recorded by

Photosynthesis genes modulate cadmium tolerance in the freshwater alga Closterium acutum revealed by transcriptome analysis
Jeongmin Shin, Han-Sol Kim, Quynh Thi Nhu Bui, Taehee Kim, Jang-Seu Ki
Journal of Applied Phycology.2025; 37(3): 1951. CrossRef
Optimization of trace metal composition utilizing Taguchi orthogonal array enhances biomass and superoxide dismutase production in Tetraselmis chuii under mixotrophic condition: implications for antioxidant formulations
Chiu Peng Chan, Huang Zhen Ni, Sangkaran Pannerchelvan, Murni Halim, Joo Shun Tan, Nor Azman Kasan, Mohd Shamzi Mohamed
International Microbiology.2025;[Epub] CrossRef
Metabolic Responses, Uptake, and Export of Copper in Cyanobacteria
Jean Coutinho Oder, Thamires Emidio Sateles, Laila Barros de Souza, Adriano Nunes-Nesi, Wagner L. Araújo, Luna Alvarenga-Lucius
Biology.2025; 14(7): 798. CrossRef
Photosynthetic and antioxidant responses of Ankistrodesmus flexuosus and Curvastrum pantanale to environmentally relevant copper concentrations
Suleiman Dauda, Ana Teresa Lombardi
Physiologia Plantarum.2024;[Epub] CrossRef
Molecular characterization of a catalase gene in the freshwater green alga Closterium ehrenbergii and its putative function against abiotic stresses
Hui Wang, Peiling Wu, Fengru Li, Jeongmin Shin, Jang-Seu Ki
European Journal of Protistology.2024; 95: 126111. CrossRef
Two novel glutathione S-transferase (GST) genes in the toxic marine dinoflagellate Alexandrium pacificum and their transcriptional responses to environmental contaminants
Hyunjun Park, Han-Sol Kim, Sofia Abassi, Quynh Thi Nhu Bui, Jang-Seu Ki
Science of The Total Environment.2024; 915: 169983. CrossRef
Sub‐lethal effects of metals and pesticides on the freshwater dinoflagellate Palatinus apiculatus and environmental implications
Quynh Thi Nhu Bui, Taehee Kim, Han‐Sol Kim, Seokmin Lee, Seungjun Lee, Jang‐Seu Ki
Water Environment Research.2024;[Epub] CrossRef
Comparative assessment of algaecide performance on freshwater phytoplankton: Understanding differential sensitivities to frame cyanobacteria management
Malihe Mehdizadeh Allaf, Kevin J. Erratt, Hassan Peerhossaini
Water Research.2023; 234: 119811. CrossRef
Assessment of Membrane and Electrochemical Technologies for the Treatment of a Selenium-Bearing Mine water: Technology Performance and Effect on Toxicity and Bioaccumulation
Mohammadali Baghbanzadeh, Sylvain Dillon, Konstantin Volchek, Carrie Rickwood, Richard R. Goulet
Mine Water and the Environment.2023; 42(3): 361. CrossRef
Toxic effect of copper on soil microalgae: experimental data and critical review
Y. Maltsev, S. Maltseva, M. Kulikovskiy
International Journal of Environmental Science and Technology.2023; 20(10): 10903. CrossRef
Molecular cloning and oxidative‐stress responses of a novel Phi class glutathione S‐transferase (GSTF) gene in the freshwater algae Closterium ehrenbergii
Sofia Abassi, Hui Wang, Hansol Kim, Jang‐Seu Ki
Environmental Toxicology.2022; 37(4): 789. CrossRef
The effects of copper ions and copper nanomaterials on the output of amino acids from marine microalgae
Wenqiu Huang, Yuping Zhou, Ting Zhao, Liju Tan, Jiangtao Wang
Environmental Science and Pollution Research.2022; 29(7): 9780. CrossRef
Heavy metal–induced stress in eukaryotic algae—mechanisms of heavy metal toxicity and tolerance with particular emphasis on oxidative stress in exposed cells and the role of antioxidant response
Beatrycze Nowicka
Environmental Science and Pollution Research.2022; 29(12): 16860. CrossRef
Synthesis, characterisation and cytotoxicity of gold microwires for ultra-sensitive biosensor development
Nurul Akmal Che Lah, Robert Gray, Sonia Trigueros
Microbial Cell Factories.2021;[Epub] CrossRef
Transcriptomic sequencing reveals the response of Dunaliella salina to copper stress via the increased photosynthesis and carbon mechanism
Na Ling, Weilu Li, Guiguo Xu, Zheng Qi, Chenfeng Ji, Xiaorui Liu, Di Cui, Yuan Sun
Molecular Omics.2021; 17(5): 769. CrossRef
Evaluation of the operational conditions in the production and morphology of Chlorella sp.
L. Rendón-Castrillón, M. Ramírez-Carmona, C. Ocampo-López, R. Giraldo-Aristizabal
Brazilian Journal of Biology.2021; 81(1): 202. CrossRef
Self-polishing emulsion platforms: Eco-friendly surface engineering of coatings toward water borne marine antifouling
Jianbin Zhang, Yizhe Liu, Xingwei Wang, Chaoyang Zhang, Hui Liu, Wufang Yang, Meirong Cai, Xiaowei Pei, Feng Zhou
Progress in Organic Coatings.2020; 149: 105945. CrossRef
Molecular identification, differential expression and protective roles of iron/manganese superoxide dismutases in the green algae Closterium ehrenbergii against metal stress
Hui Wang, Jang-Seu Ki
European Journal of Protistology.2020; 74: 125689. CrossRef
Molecular characterization and expression analysis of copper‐zinc superoxide dismutases from the freshwater alga Closterium ehrenbergii under metal stress
Hui Wang, Jang‐Seu Ki
Environmental Toxicology.2020; 35(1): 5. CrossRef
Effect of polymeric biocide polyhexamethylene guanidine hydrochloride on morpho-physiological and biochemical parameters of wheat seedlings under copper stress
L. Lyoshyna, O. Tarasyuk, O. Bulko, S. Rogalsky, T. Kamenieva, M. Kuchuk
Agricultural Science and Practice.2020; 7(1): 49. CrossRef
The Growth, Population Size Structure, Physiological Changes, and Ultrastructure of the Microalga Picochlorum maculatum (Chlorophyta) under Sublethal Copper Concentrations
Zh. V. Markina
Russian Journal of Marine Biology.2020; 46(5): 346. CrossRef
Genome analyses provide insights into the evolution and adaptation of the eukaryotic Picophytoplankton Mychonastes homosphaera
Changqing Liu, Xiaoli Shi, Fan Wu, Mingdong Ren, Guang Gao, Qinglong Wu
BMC Genomics.2020;[Epub] CrossRef
Small heat shock protein genes of the green algae Closterium ehrenbergii: Cloning and differential expression under heat and heavy metal stresses
Sofia Abassi, Hui Wang, Thangaraj Ponmani, Jang‐Seu Ki
Environmental Toxicology.2019; 34(9): 1013. CrossRef
Yellow clay modulates carbohydrate and glutathione responses in the harmful dinoflagellate Cochlodinium polykrikoides and leads to sedimentation
Jang-Seu Ki, Vinitha Ebenezer, Weol-Ae Lim
European Journal of Protistology.2019; 71: 125642. CrossRef
Differential transcriptional responses of carotenoid biosynthesis genes in the marine green alga Tetraselmis suecica exposed to redox and non-redox active metals
Ramaraj Sathasivam, Jang-Seu Ki
Molecular Biology Reports.2019; 46(1): 1167. CrossRef
Origin and roles of a novel copper-zinc superoxide dismutase (CuZnSOD) gene from the harmful dinoflagellate Prorocentrum minimum
Hui Wang, Sofia Abassi, Jang-Seu Ki
Gene.2019; 683: 113. CrossRef
Enhancement of growth and paramylon production of Euglena gracilis by co-cultivation with Pseudoalteromonas sp. MEBiC 03485
Min Seo Jeon, Jeong-Joo Oh, Jee Young Kim, Sang-Il Han, Sang Jun Sim, Yoon-E Choi
Bioresource Technology.2019; 288: 121513. CrossRef
Molecular cloning and oxidative-stress responses of a novel manganese superoxide dismutase (MnSOD) gene in the dinoflagellate Prorocentrum minimum
Hui Wang, Hansol Kim, Weol-Ae Lim, Jang-Seu Ki
Molecular Biology Reports.2019; 46(6): 5955. CrossRef

Application of high-salinity stress for enhancing the lipid productivity of Chlorella sorokiniana HS1 in a two-phase process: Ramesh Kakarla , Jung-Woon Choi , Jin-Ho Yun , Byung-Hyuk Kim , Jina Heo , Sujin Lee , Dae-Hyun Cho , Rishiram Ramanan , Hee-Sik Kim; J. Microbiol. 2018;56(1):56-64. Published online January 4, 2018; DOI: https://doi.org/10.1007/s12275-018-7488-6

478 View
0 Download
43 Crossref

Abstract PDF

Increased lipid accumulation of algal cells as a response to environmental stress factors attracted much attention of researchers to incorporate this stress response into industrial algal cultivation process with the aim of enhancing algal lipid productivity. This study applies high-salinity stress condition to a two-phase process in which microalgal cells are initially grown in freshwater medium until late exponential phase and subsequently subjected to high-salinity condition that induces excessive lipid accumulation. Our initial experiment revealed that the concentrated culture of Chlorella sorokiniana HS1 exhibited the intense fluorescence of Nile red at the NaCl concentration of 60 g/L along with 1 g/L of supplemental bicarbonate after 48 h of induction period without significantly compromising cultural integrity. These conditions were further verified with the algal culture grown for 7 days in a 1 L bottle reactor that reached late exponential phase; a 12% increment in the lipid content of harvested biomass was observed upon inducing high lipid accumulation in the concentrated algal culture at the density of 5.0 g DW/L. Although an increase in the sum of carbohydrate and lipid contents of harvested biomass indicated that the external carbon source supplemented during the induction period increased overall carbon assimilation, a decrease in carbohydrate content suggested the potential reallocation of cellular carbon that promoted lipid droplet formation under high-salinity stress. These
results
thus emphasize that the two-phase process can be successfully implemented to enhance algal lipid productivity by incorporating high-salinity stress conditions into the pre-concentrated sedimentation ponds of industrial algal production system.

Citations

Citations to this article as recorded by

Synergizing food waste management and microalgae biorefinery for bioenergy production: Recent advance on direct and indirect conversion pathways
Adityas Agung Ramandani, Sze Ying Lee, Anet Režek Jambrak, Wei-Hsin Chen, Jun Wei Lim, Kuan Shiong Khoo
Process Biochemistry.2025; 151: 14. CrossRef
Harnessing the potential of Chlorella vulgaris in salmon industry wastewater treatment: The role of salinity type in optimizing biorefinery processes
Cynthia Urrutia Molina, María Eugenia González Quijón, Erwin Yañez, Claudio R. Navarro, Camilo Rodríguez-Villegas, David Silva
Algal Research.2025; 88: 103999. CrossRef
Effects of hyposalinity stress on the physiological state of the marine microalgae Coccomyxa parasitica
E.S. Kladchenko, D.S. Lavrichenko, E.A. Bogacheva, E.S. Chelebieva
Regional Studies in Marine Science.2025; 90: 104443. CrossRef
Bioremediation of dissolved organic compounds in produced water from oil and gas operations using Chlorella sorokiniana: a sustainable approach
Mohammed Omar Faruque, Tariq Mohammed, Mohammad Mozahar Hossain, Shaikh Abdur Razzak
Environmental Monitoring and Assessment.2024;[Epub] CrossRef
Optimization of iron, phosphate, and salinity in nutrient medium using response surface methodology for enhancing biochemical composition in Chlorella sp. culture
Jurmin H. Sarri, Mahmut Elp
Algal Research.2024; 84: 103747. CrossRef
Long-term acclimation to organic carbon enhances the production of loliolide from Scenedesmus deserticola
Dae-Hyun Cho, Jin-Ho Yun, Dong-Yoon Choi, Jina Heo, Eun Kyung Kim, Juran Ha, Chan Yoo, Hong Il Choi, Yong Jae Lee, Hee-Sik Kim
Bioresource Technology.2024; 412: 131408. CrossRef
An Overview of Green Bioprocessing of Algae-Derived Biochar and Biopolymers: Synthesis, Preparation, and Potential Applications
Motasem Y. D. Alazaiza, Ahmed Albahnasawi, Murat Eyvaz, Tahra Al Maskari, Dia Eddin Nassani, Salem S. Abu Amr, Mohammed Shadi S. Abujazar, Mohammed J. K. Bashir
Energies.2023; 16(2): 791. CrossRef
A review of microalgal cell wall composition and degradation to enhance the recovery of biomolecules for biofuel production
Syafiqah Md Nadzir, Norjan Yusof, Norazela Nordin, Azlan Kamari, Mohd Zulkhairi Mohd Yusoff
Biofuels.2023; 14(9): 979. CrossRef
Enhanced mixotrophic production of lutein and lipid from potential microalgae isolate Chlorella sorokiniana C16
Anil Kumar Patel, Akash Pralhad Vadrale, Reeta-Rani Singhania, Chiu-Wen Chen, Jo Shu Chang, Cheng-Di Dong
Bioresource Technology.2023; 386: 129477. CrossRef
Potential of Halophytes as Sustainable Fodder Production by Using Saline Resources: A Review of Current Knowledge and Future Directions
Maria Hasnain, Zainul Abideen, Faraz Ali, Mirza Hasanuzzaman, Ali El-Keblawy
Plants.2023; 12(11): 2150. CrossRef
Salinity stress provokes diverse physiological responses of eukaryotic unicellular microalgae
Attila Farkas, Bernadett Pap, Ottó Zsíros, Roland Patai, Prateek Shetty, Győző Garab, Tibor Bíró, Vince Ördög, Gergely Maróti
Algal Research.2023; 73: 103155. CrossRef
Utilization of Saline Water Enhances Lipid Accumulation in Green Microalgae for the Sustainable Production of Biodiesel
Maria Hasnain, Zainul Abideen, Daniel Anthony Dias, Shagufta Naz, Neelma Munir
BioEnergy Research.2023; 16(2): 1026. CrossRef
Cultivation of Freshwater Microalgae in Wastewater Under High Salinity for Biomass, Nutrients Removal, and Fatty Acids/Biodiesel Production
Qi Yang, Min Zhang, Hend A. Alwathnani, Muhammad Usman, Badr A. Mohamed, Abd El-Fatah Abomohra, El-Sayed Salama
Waste and Biomass Valorization.2022; 13(7): 3245. CrossRef
Overview on stress-induced strategies for enhanced microalgae lipid production: Application, mechanisms and challenges
Xueting Song, Bing-Feng Liu, Fanying Kong, Nan-Qi Ren, Hong-Yu Ren
Resources, Conservation and Recycling.2022; 183: 106355. CrossRef
Biodesalination of brackish water coupled with lipid production using native Scenedesmus sp. isolated from a saline lake in Ethiopia, Lake Basaka
Muluwork Maru, Feleke Zewge, Demeke Kifle, Endalkachew Sahle-Demissie
Desalination and Water Treatment.2022; 266: 39. CrossRef
Anaerobic digestates grown oleaginous microalgae for pollutants removal and lipids production
Xiao-Bo Tan, Ya-Lei Zhang, Xian-Chao Zhao, Li-Bin Yang, Shun-Cheng Yangwang, Yue Zou, Jue-Ming Lu
Chemosphere.2022; 308: 136177. CrossRef
A critical review on employing algae as a feed for polycarbohydrate synthesis
V S Lisha, Rushikesh S. Kothale, Sumati Sidharth, Balasubramanian Kandasubramanian
Carbohydrate Polymer Technologies and Applications.2022; 4: 100242. CrossRef
Salinity induces unique changes in lipid classes and fatty acids of the estuarine haptophyte Diacronema vlkianum
José-Pedro Cañavate, Catalina Fernández-Díaz
European Journal of Phycology.2022; 57(3): 297. CrossRef
Algal biopolymers as sustainable resources for a net-zero carbon bioeconomy
A. Aswathi Mohan, Aiswarya Robert Antony, Kozhumal Greeshma, Jin-Ho Yun, Rishiram Ramanan, Hee-Sik Kim
Bioresource Technology.2022; 344: 126397. CrossRef
An appraisal of the variable response of microalgal lipids to culture salinity
José‐Pedro Cañavate, Catalina Fernández‐Díaz
Reviews in Aquaculture.2022; 14(1): 192. CrossRef
Two-stage cultivation of Chlorella vulgaris using light and salt stress conditions for simultaneous production of lipid, carotenoids, and antioxidants
Hamdy Elsayed Ahmed Ali, Eman A. El-fayoumy, Wessam E. Rasmy, Ramadan M. Soliman, Mohd Azmuddin Abdullah
Journal of Applied Phycology.2021; 33(1): 227. CrossRef
Evaluation of the operational conditions in the production and morphology of Chlorella sp.
L. Rendón-Castrillón, M. Ramírez-Carmona, C. Ocampo-López, R. Giraldo-Aristizabal
Brazilian Journal of Biology.2021; 81(1): 202. CrossRef
Historical occurrences of marine microalgal blooms in Indian peninsula: Probable causes and implications
Oyeshina Gideon Oyeku, Subir Kumar Mandal
Oceanologia.2021; 63(1): 51. CrossRef
Sodium chloride and nitrogen effects on Chlorella vulgaris growth and biocommodities production
Styliani E. Biliani, Ioannis D. Manariotis
Desalination and Water Treatment.2021; 237: 159. CrossRef
Two-stage cultivation of microalgae for production of high-value compounds and biofuels: A review
Vinoj Chamilka Liyanaarachchi, Malith Premaratne, Thilini U. Ariyadasa, P.H.V. Nimarshana, Anushree Malik
Algal Research.2021; 57: 102353. CrossRef
The effect on single and combined stresses for biomass and lipid production from Nannochloris atomus using two phase culture system
So Hee Kim, Clovis Awah Che, Gwi-Taek Jeong, Sung-Koo Kim
Journal of Biotechnology.2021; 326: 40. CrossRef
Perspective Design of Algae Photobioreactor for Greenhouses—A Comparative Study
Kateřina Sukačová, Pavel Lošák, Vladimír Brummer, Vítězslav Máša, Daniel Vícha, Tomáš Zavřel
Energies.2021; 14(5): 1338. CrossRef
Development of mutant microalgae that accumulate lipids under nitrate-replete conditions
Tomoki Oyama, Yuichi Kato, Katsuya Satoh, Yutaka Oono, Mami Matsuda, Tomohisa Hasunuma, Akihiko Kondo
Algal Research.2021; 60: 102544. CrossRef
Salinity-induced microalgal-based mariculture wastewater treatment combined with biodiesel production
Chaofan Zhang, Tomohisa Hasunuma, Su Shiung Lam, Akihiko Kondo, Shih-Hsin Ho
Bioresource Technology.2021; 340: 125638. CrossRef
Cultivation and biochemical characterization of isolated Sicilian microalgal species in salt and temperature stress conditions
Rosaria Arena, Serena Lima, Valeria Villanova, Nadia Moukri, Eleonora Curcuraci, Concetta Messina, Andrea Santulli, Francesca Scargiali
Algal Research.2021; 59: 102430. CrossRef
Transcriptomic analysis of Chlorella sp. HS2 suggests the overflow of acetyl‐CoA and NADPH cofactor induces high lipid accumulation and halotolerance
Jin‐Ho Yun, Michaël Pierrelée, Dae‐Hyun Cho, Urim Kim, Jina Heo, Dong‐Yun Choi, Yong Jae Lee, Bongsoo Lee, HyeRan Kim, Bianca Habermann, Yong Keun Chang, Hee‐Sik Kim
Food and Energy Security.2021;[Epub] CrossRef
Utilization of the acid hydrolysate of defatted Chlorella biomass as a sole fermentation substrate for the production of biosurfactant from Bacillus subtilis C9
Jin-Ho Yun, Dae-Hyun Cho, Bongsoo Lee, Yong Jae Lee, Dong-Yun Choi, Hee-Sik Kim, Yong Keun Chang
Algal Research.2020; 47: 101868. CrossRef
Two-stage cultivation strategy for simultaneous increases in growth rate and lipid content of microalgae: A review
Md Maniruzzaman A. Aziz, Khairul Anuar Kassim, Zahra Shokravi, Fauzan Mohd Jakarni, Hong Yuan Liu, Nabilah Zaini, Lian See Tan, A.B.M. Saiful Islam, Hoofar Shokravi
Renewable and Sustainable Energy Reviews.2020; 119: 109621. CrossRef
Exploring the distinctiveness of biomass and biomolecules from limnic microalgae of unexplored waters of Noyyal River, Western Ghats, for exploitation
Pandian Sureshkumar, Jibu Thomas
Environmental Science and Pollution Research.2020; 27(18): 23309. CrossRef
Enhanced lipid productivity of Chlamydomonas reinhardtii with combination of NaCl and CaCl2 stresses
Le Thai Hang, Kazuhiro Mori, Yasuhiro Tanaka, Masaaki Morikawa, Tadashi Toyama
Bioprocess and Biosystems Engineering.2020; 43(6): 971. CrossRef
Dynamic Interactions between Autophagosomes and Lipid Droplets in Chlamydomonas reinhardtii
Quynh-Giao Tran, Hyang Ran Yoon, Kichul Cho, Seon-Jin Lee, José L. Crespo, Rishiram Ramanan, Hee-Sik Kim
Cells.2019; 8(9): 992. CrossRef
Evaluation of the potential of Chlorella sp. HS2, an algal isolate from a tidal rock pool, as an industrial algal crop under a wide range of abiotic conditions
Jin-Ho Yun, Dae-Hyun Cho, Jina Heo, Yong Jae Lee, Bongsoo Lee, Yong Keun Chang, Hee-Sik Kim
Journal of Applied Phycology.2019; 31(4): 2245. CrossRef
Maximizing biomass and lipid production in Ettlia sp. by ultraviolet stress in a continuous culture
Seong-Hyun Seo, Ankita Srivastava, Myung-Soo Han, Hyung-Gwan Lee, Hee-Mock Oh
Bioresource Technology.2019; 288: 121472. CrossRef
Identification of significant proxy variable for the physiological status affecting salt stress-induced lipid accumulation in Chlorella sorokiniana HS1
Seung Hwan Oh, Yong Keun Chang, Jay Hyung Lee
Biotechnology for Biofuels.2019;[Epub] CrossRef
Isolation and genome analysis of Winogradskyella algicola sp. nov., the dominant bacterial species associated with the green alga Dunaliella tertiolecta
Jaeho Song, Yeonjung Lim, Hye-Jin Jang, Yochan Joung, Ilnam Kang, Seong-Joo Hong, Choul-Gyun Lee, Jang-Cheon Cho
Journal of Microbiology.2019; 57(11): 982. CrossRef
Nitrogen modulation under chemostat cultivation mode induces biomass and lipid production by Chlorella vulgaris and reduces antenna pigment accumulation
Kichul Cho, Dae-Hyun Cho, Jina Heo, Urim Kim, Yong Jae Lee, Dong-Yun Choi, Hee-Sik Kim
Bioresource Technology.2019; 281: 118. CrossRef
Optimization of microalgal growth and cultivation parameters for increasing bioenergy potential: Case study using the oleaginous microalga Chlorella pyrenoidosa Chick (IPPAS C2)
Kateřina Sukačová, Diana Búzová, Petr Trávníček, Jan Červený, Monika Vítězová, Tomáš Vítěz
Algal Research.2019; 40: 101519. CrossRef
Application of biosurfactant from Bacillus subtilis C9 for controlling cladoceran grazers in algal cultivation systems
Jin-Ho Yun, Dae-Hyun Cho, Bongsoo Lee, Hee-Sik Kim, Yong Keun Chang
Scientific Reports.2018;[Epub] CrossRef

Effect of amikacin on cell wall glycopeptidolipid synthesis in Mycobacterium abscessus: So-Young Lee , Hee-Youn Kim , Byoung-Jun Kim , Hong Kim , Seung-hyeok Seok , Bum-Joon Kim , Yoon-Hoh Kook; J. Microbiol. 2017;55(8):640-647. Published online July 28, 2017; DOI: https://doi.org/10.1007/s12275-017-6503-7

329 View
0 Download
7 Crossref

Abstract PDF

Cultivation of the smooth colony Mycobacterium abscessus at the sub-minimum inhibitory concentration (MIC) of amikacin changed its growth pattern including its colony morphology (smooth to rough) and cell arrangement (dispersed to cord formation). In addition, reduced sliding motility and biofilm formation were observed. The amount of glycogpetidolipid (GPL) and mRNA expression of key genes involved in GPL synthesis were decreased in the amikacin-treated M. abscessus strain. An in vitro infection assay revealed that the amikacin-treated smooth M. abscessus strain induced more pro-inflammatory cytokines (TNF-α, IL-1β, IL-6) than that of the smooth strain in murine macrophage cells. These results suggest that long-term exposure to a low concentration of amikacin causes a physical change in the cell wall which may increase its virulence.

Citations

Citations to this article as recorded by

Drugs for treating infections caused by non-tubercular mycobacteria: a narrative review from the study group on mycobacteria of the Italian Society of Infectious Diseases and Tropical Medicine
A. Calcagno, N. Coppola, L. Sarmati, M. Tadolini, R. Parrella, A. Matteelli, N. Riccardi, M. Trezzi, A. Di Biagio, V. Pirriatore, A. Russo, G. Gualano, E. Pontali, L. Surace, E. Falbo, J. Mencarini, F. Palmieri, A. Gori, M. Schiuma, G. Lapadula, D. Golett
Infection.2024; 52(3): 737. CrossRef
Moles of Molecules against Mycobacterium abscessus: A Review of Current Research
Mario Cocorullo, Christian Bettoni, Sara Foiadelli, Giovanni Stelitano
Future Pharmacology.2023; 3(3): 637. CrossRef
Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2017–2018
David J. Harvey
Mass Spectrometry Reviews.2023; 42(1): 227. CrossRef
The role of amikacin in the treatment of nontuberculous mycobacterial disease
Jelmer Raaijmakers, Jodie Anne Schildkraut, Wouter Hoefsloot, Jakko van Ingen
Expert Opinion on Pharmacotherapy.2021; 22(15): 1961. CrossRef
Integrated Quantitative Proteomics and Metabolome Profiling Reveal MSMEG_6171 Overexpression Perturbing Lipid Metabolism of Mycobacterium smegmatis Leading to Increased Vancomycin Resistance
Zhuhua Wu, Wenjing Wei, Ying Zhou, Huixin Guo, Jiao Zhao, Qinghua Liao, Liang Chen, Xiaoli Zhang, Lin Zhou
Frontiers in Microbiology.2020;[Epub] CrossRef
Glycopeptidolipids, a Double-Edged Sword of the Mycobacterium abscessus Complex
Ana Victoria Gutiérrez, Albertus Viljoen, Eric Ghigo, Jean-Louis Herrmann, Laurent Kremer
Frontiers in Microbiology.2018;[Epub] CrossRef
Role of the Mycobacterium marinum ESX-1 Secretion System in Sliding Motility and Biofilm Formation
Li-Yin Lai, Tzu-Lung Lin, Yi-Yin Chen, Pei-Fang Hsieh, Jin-Town Wang
Frontiers in Microbiology.2018;[Epub] CrossRef

A rapid and simple method for identifying bacterial polar lipid components in wet biomass: Tuan Manh Nguyen , Jaisoo Kim; J. Microbiol. 2017;55(8):635-639. Published online July 4, 2017; DOI: https://doi.org/10.1007/s12275-017-7092-1

381 View
0 Download
24 Crossref

Abstract PDF

There are marked differences between wet and freeze-dried cells with regard to the identification of polar lipid components. The determination of the polar lipid composition of freeze-dried cells is well established. However, several approaches to identifying polar lipid components in wet cells have met with limited success owing to the presence of non-polar compounds in the extracts, resulting in a lipid composition with a narrow scope. In this study, we surveyed the lipid profiles of the wet biomasses of three Gram-positive (Microbacterium lacticum, Rhodococcus koreensis, and Streptomyces longwoodensis) and two Gram-negative (Pseudomonas aeruginosa and Novosphingobium capsulatum) bacteria; the results were comparable in quality to those obtained using a standard freeze-dried approach. Moreover, our improved method ensures simple lipid extraction. Overall, the results of the analysis showed minor lipid profile differences between the two approaches with regard to quantity, and lipid identification was consistent in both methods for all species.

Citations

Citations to this article as recorded by

Streptomyces lothianensis sp. nov., an Antibiotic‐Producing Strain Isolated From the Indian Sundarbans Mangrove Forest
Pankaj Paul, Kaushik Biswas, Sergio de los Santos Villalobos, Maria Edith Ortega Urquieta, Dhruba Bhattacharya, Sanmoy Karmakar, Kamalika Mazumder, Hariom Kushwaha
Journal of Basic Microbiology.2025;[Epub] CrossRef
Amycolatopsis mongoliensis sp. nov., a novel actinobacterium with antifungal activity isolated from a coal mining site in Mongolia
Bilguun Oyuntsetseg, Hyang Burm Lee, Seung Bum Kim
International Journal of Systematic and Evolutionary Microbiology .2024;[Epub] CrossRef
Amycolatopsis nalaikhensis sp. nov. and Amycolatopsis carbonis sp. nov., two novel actinobacteria with antimicrobial activity isolated from a coal mining site in Mongolia
Bilguun Oyuntsetseg, Seung Bum Kim
International Journal of Systematic and Evolutionary Microbiology .2024;[Epub] CrossRef
Isoptericola haloaureus sp. nov., a dimorphic actinobacterium isolated from mangrove sediments of southeast India, implicating biosaline agricultural significance through nitrogen fixation and salt tolerance genes
Munisamy Prathaban, Ragothaman Prathiviraj, Mythili Ravichandran, Sharmila Devi Natarajan, Murugesan Sobanaa, S. Hari Krishna Kumar, Varadharaju Chandrasekar, Joseph Selvin
Antonie van Leeuwenhoek.2024;[Epub] CrossRef
Quorum Quenching Potential of Reyranella sp. Isolated from Riverside Soil and Description of Reyranella humidisoli sp. nov.
Dong Hyeon Lee, Seung Bum Kim
Journal of Microbiology.2024; 62(6): 449. CrossRef
Mycovorax composti gen. nov., sp. nov., a member of the family Chitinophagaceae isolated from button mushroom compost
Meghann Thai, Tina L. Bell, Michael A. Kertesz
International Journal of Systematic and Evolutionary Microbiology .2024;[Epub] CrossRef
Description of Chryseobacterium fluminis sp. nov., a keratinolytic bacterium isolated from a freshwater river
Moonsoo Kim, Eun Tak Oh, Seung Bum Kim
International Journal of Systematic and Evolutionary Microbiology .2024;[Epub] CrossRef
Shewanella metallivivens sp. nov., a deep-sea hydrothermal vent tube worm endobiont capable of dissimilatory anaerobic metalloid oxyanion reduction
Chris Maltman, Steven B. Kuzyk, John A. Kyndt, George Lengyel, Vladimir Yurkov
International Journal of Systematic and Evolutionary Microbiology .2023;[Epub] CrossRef
Flavobacterium humidisoli sp. nov., isolated from riverside soil
Jun Sik Ra, Eun Tak Oh, Ji-Hye Han, Seung Bum Kim
International Journal of Systematic and Evolutionary Microbiology .2023;[Epub] CrossRef
Description of Microcella humidisoli sp. nov. and Microcella daejeonensis sp. nov., isolated from riverside soil, reclassification of Marinisubtilis pacificus as Microcella pacifica comb. nov., and emended description of the genus Microcella
Katya Michelle Molina Ayala, Seung Bum Kim
International Journal of Systematic and Evolutionary Microbiology .2023;[Epub] CrossRef
Comparing 16S rRNA gene similarity with simple polar lipids profiling amongst Salmonella isolates
IM T Fadlalla, ME Hamid, AG A Rahim, ED M Elamin
Nigerian Journal of Health Sciences.2023; 23(1): 10. CrossRef
Characterization of Streptomyces nymphaeiformis sp. nov., and its taxonomic relatedness to other polyhydroxybutyrate-degrading streptomycetes
Gary R. Hix, Muhammad S. Khan, Mikayla T. Miller, Elisha C. Napier, Allison L. O'Brien, Roger P. White, Stephen F. Baron
International Journal of Systematic and Evolutionary Microbiology .2022;[Epub] CrossRef
Micromonospora antibiotica sp. nov. and Micromonospora humidisoli sp. nov., two new actinobacterial species exhibiting antimicrobial potential
Dong Hyeon Lee, Jun Sik Ra, Min Ji Kim, Seung Bum Kim
International Journal of Systematic and Evolutionary Microbiology .2022;[Epub] CrossRef
Colwellia maritima sp. nov. and Polaribacter marinus sp. nov., isolated from seawater
Sylvia Kristyanto, Jaejoon Jung, Jeong Min Kim, Keunpil Kim, Mi-hwa Lee, Lujiang Hao, Che Ok Jeon
International Journal of Systematic and Evolutionary Microbiology .2022;[Epub] CrossRef
Chryseobacterium pennae sp. nov., isolated from poultry feather waste
Adeline Lum Nde, George Charimba, Arina Hitzeroth, Lize Oosthuizen, Laurinda Steyn, Jeffrey D. Newman, Celia Hugo
International Journal of Systematic and Evolutionary Microbiology .2021;[Epub] CrossRef
Myceligenerans indicum sp. nov., an actinobacterium isolated from mangrove sediment of Sundarbans, India
Kannan Asha, Punyasloke Bhadury
Archives of Microbiology.2021; 203(4): 1577. CrossRef
Flavobacterium solisilvae sp. nov. and Flavobacterium silvaticum sp. nov., isolated from forest soil
Hye Su Jung, Byung Hee Chun, Hyung Min Kim, Che Ok Jeon
International Journal of Systematic and Evolutionary Microbiology .2021;[Epub] CrossRef
Ciceribacter ferrooxidans sp. nov., a nitrate-reducing Fe(II)-oxidizing bacterium isolated from ferrous ion-rich sediment
Tongchu Deng, Youfen Qian, Xingjuan Chen, Xunan Yang, Jun Guo, Guoping Sun, Meiying Xu
Journal of Microbiology.2020; 58(5): 350. CrossRef
Paenibacillus piri sp. nov., isolated from urban soil
Ngoc Hoang Trinh, Jaisoo Kim
International Journal of Systematic and Evolutionary Microbiology.2020; 70(1): 656. CrossRef
Psychrobacillus glaciei sp. nov., a psychrotolerant species isolated from an Antarctic iceberg
Jun Young Choi, Pyung Cheon Lee
International Journal of Systematic and Evolutionary Microbiology .2020; 70(3): 1947. CrossRef
Sphingobium aromaticivastans sp. nov., a novel aniline- and benzene-degrading, and antimicrobial compound producing bacterium
Tuan Manh Nguyen, Jaisoo Kim
Archives of Microbiology.2019; 201(2): 155. CrossRef
Flavobacterium ureilyticum sp. nov., a novel urea hydrolysing bacterium isolated from stream bank soil
Ram Hari Dahal, Jaisoo Kim
Antonie van Leeuwenhoek.2018; 111(11): 2131. CrossRef
Proposal of three novel species of soil bacteria, Variovorax ureilyticus, Variovorax rhizosphaerae, and Variovorax robiniae, in the family Comamonadaceae
Tuan Manh Nguyen, Ngoc Hoang Trinh, Jaisoo Kim
Journal of Microbiology.2018; 56(7): 485. CrossRef
Characterization of Flavobacterium aquimarinum sp. nov., a halotolerant bacterium isolated from seawater
Sylvia Kristyanto, Tuan Manh Nguyen, Dhiraj Kumar Chaudhary, Sang-Seob Lee, Jaisoo Kim
Journal of Microbiology.2018; 56(5): 317. CrossRef

The protein and neutral lipid composition of lipid droplets isolated from the fission yeast, Schizosaccharomyces pombe: Alex Meyers , Karuna Chourey , Taylor M. Weiskittel , Susan Pfiffner , John R. Dunlap , Robert L. Hettich , Paul Dalhaimer; J. Microbiol. 2017;55(2):112-122. Published online January 26, 2017; DOI: https://doi.org/10.1007/s12275-017-6205-1

338 View
0 Download
16 Crossref

Abstract PDF

Lipid droplets consist of a core of neutral lipids surrounded by a phospholipid monolayer with bound proteins. Much of the information on lipid droplet function comes from proteomic and lipodomic studies that identify the components of droplets isolated from organisms throughout the phylogenetic tree. Here, we add to that important inventory by reporting lipid droplet factors from the fission yeast, Schizosaccharomyces pombe. Unique to this study was the fact that cells were cultured in three different environments: 1) late log growth phase in glucose-based media, 2) stationary phase in glucosebased media, and 3) late log growth phase in media containing oleic acid. We confirmed colocalization of major factors with lipid droplets using live-cell fluorescent microscopy. We also analyzed droplets from each of the three conditions for sterol ester (SE) and triacylglycerol (TAG) content, along with their respective fatty acid compositions. We identified a previously undiscovered lipid droplet protein, Vip1p, which affects droplet size distribution. The results provide further insight into the workings of these ubiquitous organelles.

Citations

Citations to this article as recorded by

Cbf11 and Mga2 function together to activate transcription of lipid metabolism genes and promote mitotic fidelity in fission yeast
Anna Marešová, Michaela Grulyová, Miluše Hradilová, Viacheslav Zemlianski, Jarmila Princová, Martin Převorovský, Cathy Savage-Dunn
PLOS Genetics.2024; 20(12): e1011509. CrossRef
Mild Heat Stress Alters the Physical State and Structure of Membranes in Triacylglycerol-Deficient Fission Yeast, Schizosaccharomyces pombe
Péter Gudmann, Imre Gombos, Mária Péter, Gábor Balogh, Zsolt Török, László Vígh, Attila Glatz
Cells.2024; 13(18): 1543. CrossRef
Lipid droplets: a cellular organelle vital in cancer cells
Yi Jin, Yanjie Tan, Jian Wu, Zhuqing Ren
Cell Death Discovery.2023;[Epub] CrossRef
Oleaginous yeasts: Time to rethink the definition?
José Manuel Salvador López, Meriam Vandeputte, Inge N. A. Van Bogaert
Yeast.2022; 39(11-12): 553. CrossRef
Proteomic and lipidomic analyses of lipid droplets in Aurantiochytrium limacinum ATCC MYA-1381
Kohei Yoneda, Yohei Ishibashi, Masaki Yoshida, Makoto M. Watanabe, Makoto Ito, Iwane Suzuki
Algal Research.2022; 67: 102844. CrossRef
ER-localized phosphatidylethanolamine synthase plays a conserved role in lipid droplet formation
Mehmet Oguz Gok, Natalie Ortiz Speer, W. Mike Henne, Jonathan R. Friedman, James Olzmann
Molecular Biology of the Cell.2022;[Epub] CrossRef
Lipid Droplet Nucleation
Abdou Rachid Thiam, Elina Ikonen
Trends in Cell Biology.2021; 31(2): 108. CrossRef
Metabolism of Storage Lipids and the Role of Lipid Droplets in the Yeast Schizosaccharomyces pombe
Ivan Hapala, Peter Griac, Roman Holic
Lipids.2020; 55(5): 513. CrossRef
Lipid Droplets in Neurodegenerative Disorders
Brandon C. Farmer, Adeline E. Walsh, Jude C. Kluemper, Lance A. Johnson
Frontiers in Neuroscience.2020;[Epub] CrossRef
Harnessing the Power of Mutagenesis and Adaptive Laboratory Evolution for High Lipid Production by Oleaginous Microalgae and Yeasts
Neha Arora, Hong-Wei Yen, George P. Philippidis
Sustainability.2020; 12(12): 5125. CrossRef
Mechanisms of protein targeting to lipid droplets: A unified cell biological and biophysical perspective
Ravi Dhiman, Stefanie Caesar, Abdou Rachid Thiam, Bianca Schrul
Seminars in Cell & Developmental Biology.2020; 108: 4. CrossRef
The New Face of the Lipid Droplet: Lipid Droplet Proteins
Congyan Zhang, Pingsheng Liu
PROTEOMICS.2019;[Epub] CrossRef
Effect of Selenium on Lipid and Amino Acid Metabolism in Yeast Cells
Marek Kieliszek, Stanisław Błażejak, Anna Bzducha-Wróbel, Anna M. Kot
Biological Trace Element Research.2019; 187(1): 316. CrossRef
The Peroxygenase Activity of the Aspergillus flavus Caleosin, AfPXG, Modulates the Biosynthesis of Aflatoxins and Their Trafficking and Extracellular Secretion via Lipid Droplets
Abdulsamie Hanano, Mari Alkara, Ibrahem Almousally, Mouhnad Shaban, Farzana Rahman, Mehedi Hassan, Denis J. Murphy
Frontiers in Microbiology.2018;[Epub] CrossRef
Mitotic defects in fission yeast lipid metabolism ‘cut’ mutants are suppressed by ammonium chloride
Róbert Zach, Jarmila Tvarůžková, Martin Schätz, Ondřej Ťupa, Beáta Grallert, Martin Převorovský
FEMS Yeast Research.2018;[Epub] CrossRef
Lipid Droplets: Formation to Breakdown
Alex Meyers, Taylor M. Weiskittel, Paul Dalhaimer
Lipids.2017; 52(6): 465. CrossRef

Review

REVIEW] All about that fat: Lipid modification of proteins in Cryptococcus neoformans: Felipe H. Santiago-Tirado , Tamara L. Doering; J. Microbiol. 2016;54(3):212-222. Published online February 27, 2016; DOI: https://doi.org/10.1007/s12275-016-5626-6

334 View
0 Download
10 Crossref

Abstract PDF

Lipid modification of proteins is a widespread, essential process whereby fatty acids, cholesterol, isoprenoids, phospholipids, or glycosylphospholipids are attached to polypeptides. These hydrophobic groups may affect protein structure, function, localization, and/or stability; as a consequence such modifications play critical regulatory roles in cellular systems. Recent advances in chemical biology and proteomics have allowed the profiling of modified proteins, enabling dissection of the functional consequences of lipid addition. The enzymes that mediate lipid modification are specific for both the lipid and protein substrates, and are conserved from fungi to humans. In this article we review these enzymes, their substrates, and the processes involved in eukaryotic lipid modification of proteins. We further focus on its occurrence in the fungal pathogen Cryptococcus neoformans, highlighting unique features that are both relevant for the biology of the organism and potentially important in the search for new therapies.

Citations

Citations to this article as recorded by

The anti-oomycete activity and potential mechanisms of pamidronate against development and pathogenicity in Phytophthora sojae
Xina Wu, Xiu Li, Ruilong Wang, Yan Sun, Yang Peng, Xinhang Li, Xinyu Yang
Physiological and Molecular Plant Pathology.2026; 141: 102986. CrossRef
Recapitulating the potential contribution of protein S-palmitoylation in cancer
Suchi Chaturvedi, Avinash Sonawane
Cancer and Metastasis Reviews.2025;[Epub] CrossRef
Acylation of non‐specific phospholipase C4 determines its function in plant response to phosphate deficiency
Bao Yang, Ke Zhang, Xiong Jin, Jiayu Yan, Shaoping Lu, Qingwen Shen, Lei Guo, Yueyun Hong, Xuemin Wang, Liang Guo
The Plant Journal.2021; 106(6): 1647. CrossRef
Genome Sequence, Assembly, and Characterization of the Antagonistic Yeast Candida oleophila Used as a Biocontrol Agent Against Post-harvest Diseases
Yuan Sui, Michael Wisniewski, Samir Droby, Edoardo Piombo, Xuehong Wu, Junyang Yue
Frontiers in Microbiology.2020;[Epub] CrossRef
Peeling the onion: the outer layers of Cryptococcus neoformans
Daniel P Agustinho, Liza C Miller, Lucy X Li, Tamara L Doering
Memórias do Instituto Oswaldo Cruz.2018;[Epub] CrossRef
The molecular era of protein S-acylation: spotlight on structure, mechanisms, and dynamics
María-Eugenia Zaballa, F. Gisou van der Goot
Critical Reviews in Biochemistry and Molecular Biology.2018; 53(4): 420. CrossRef
Glucosamine stimulates pheromone-independent dimorphic transition in Cryptococcus neoformans by promoting Crz1 nuclear translocation
Xinping Xu, Jianfeng Lin, Youbao Zhao, Elyssa Kirkman, Yee-Seul So, Yong-Sun Bahn, Xiaorong Lin, Aaron P. Mitchell
PLOS Genetics.2017; 13(9): e1006982. CrossRef
Alpha-helicoidal HEAT-like Repeat Proteins (αRep) Selected as Interactors of HIV-1 Nucleocapsid Negatively Interfere with Viral Genome Packaging and Virus Maturation
Sudarat Hadpech, Sawitree Nangola, Koollawat Chupradit, Kanda Fanhchaksai, Wilhelm Furnon, Agathe Urvoas, Marie Valerio-Lepiniec, Philippe Minard, Pierre Boulanger, Saw-See Hong, Chatchai Tayapiwatana
Scientific Reports.2017;[Epub] CrossRef
Human fungal pathogens: Why should we learn?
Jeong-Yoon Kim
Journal of Microbiology.2016; 54(3): 145. CrossRef
Interactions between Melanin Enzymes and Their Atypical Recruitment to the Secretory Pathway by Palmitoylation
Srijana Upadhyay, Xinping Xu, Xiaorong Lin, J. Andrew Alspaugh
mBio.2016;[Epub] CrossRef

Research Support, Non-U.S. Gov'ts

Bacillus cheonanensis sp. nov. Isolated from Near Poultry Farm Soil: Hyun-Ju Kim , Cheol-Su Park , Siwon Lee , Tae-Young Ahn; J. Microbiol. 2014;52(7):554-558. Published online May 30, 2014; DOI: https://doi.org/10.1007/s12275-014-3458-9

353 View
0 Download
1 Crossref

Abstract PDF

A novel bacterial strain, designated PFS-5T, was isolated from the soil environment with feces of a live poultry farm located in Cheonan, Republic of Korea. Strain PFS-5T was Gram-staining-positive, motile, strictly aerobic bacterium, rod-shaped, and endospore-forming. The strain contained meso-diaminopimelic acid in their peptidoglycan and MK-7 menaquinone. The major fatty acids were anteiso-C15:0 (44.2%), C16:0 (22.2%), and iso-C15:0 (16.7%). The DNA G+C content was 40.1 mol%. Comparative 16S rRNA gene sequence analysis identified strain PFS-5T in the genus Bacillus, exhibiting the highest level of sequence similarity with type strain of B. herbersteinensis D-1,5aT (96.9%), B. humi LMG 22167T (96.7%), B. alkalitelluris BA288T (96.1%), B. litoralis SW-211T (96.0%), and B. luteolus YIM93174T (95.5%). The major polar lipids of PFS-5T were diphosphatidylglycerol and phosphatidylglycerol. On the basis of result from poly-phasic data, strain PFS-5T represents a novel species, for which the name Bacillus cheonanensis sp. nov. is proposed (Type strain PFS-5T= KACC 17469T= JCM19333T).

Citations

Citations to this article as recorded by

Bacillus coreaensis sp. nov.: a xylan-hydrolyzing bacterium isolated from the soil of Jeju Island, Republic of Korea
Won-Jae Chi, Young Sang Youn, Jae-Seon Park, Soon-Kwang Hong
Journal of Microbiology.2015; 53(7): 448. CrossRef

NOTE] Pedobacter jeongneungensis sp. nov., Isolated from Forest Soil: Jaejoon Jung , Woojun Park; J. Microbiol. 2012;50(4):660-664. Published online July 21, 2012; DOI: https://doi.org/10.1007/s12275-012-1629-0

312 View
0 Download
8 Crossref

Abstract PDF

Strain BH45T was isolated from forest soil of Mt. Bukhan in Jeongneung, Seoul, Korea. The Gram-staining-negative strain BH45T grows at 4–30°C (optimum of 25–30°C) and between pH 5–8 (optimum of pH 6–8). Its major cellular fatty acids are C18:3 ω6c (6,9,12) and C10:0. The G+C content of genomic DNA was 40.2 mol%. The major respiratory quinone system in strain BH45T is menaquinone-7. Phylogenetic analysis based on 16S rRNA gene sequences indicates that strain BH45T is closely related to the genus Pedobacter. Sequence similarities with P. terrae KCTC 12762T, P. suwonensis KACC 11317T, P. soli KACC 14939T, P. alluvionis DSM 19624T, P. roseus KCCM 42272T, P. yonginense KCTC 22721T were 97.5, 97.1, 97.0, 97.0, 97.0, and 96.0%, respectively. DNA-DNA hybridization results distinguish strain BH45T from two Pedobacter species with high 16S rRNA gene sequence similarities. According to the phenotypic and molecular data, the strain BH45T clearly represents a novel species within the genus Pedobacter; thus, the name Pedobacter jeongneungensis sp. nov. is proposed for this strain. The type strain is BH45T (=KACC 15514T =JCM 17626T).

Citations

Citations to this article as recorded by

Validation List no. 222. Valid publication of new names and new combinations effectively published outside the IJSEM
Aharon Oren, Markus Göker
International Journal of Systematic and Evolutionary Microbiology .2025;[Epub] CrossRef
Pedobacter faecalis sp. nov., isolated from the faeces of eland, Taurotragus oryx
Yerim Park, Jihyeon Min, Bitnara Kim, Woojun Park
International Journal of Systematic and Evolutionary Microbiology .2024;[Epub] CrossRef
Rheinheimera faecalis sp. nov., isolated from Ceratotherium simum feces
Yerim Park, Minkyung Kim, Yeji Cha, Woojun Park
Archives of Microbiology.2023;[Epub] CrossRef
Aquibium microcysteis gen. nov., sp. nov., isolated from a Microcystis aeruginosa culture and reclassification of Mesorhizobium carbonis as Aquibium carbonis comb. nov. and Mesorhizobium oceanicum as Aquibium oceanicum comb. nov
Minkyung Kim, Wonjae Kim, Woojun Park
International Journal of Systematic and Evolutionary Microbiology .2022;[Epub] CrossRef
Niveibacterium microcysteis sp. nov., isolated from a cyanobacterial bloom sample
Mingyeong Kang, Woojun Park
International Journal of Systematic and Evolutionary Microbiology .2021;[Epub] CrossRef
Pedobacter ghigonii sp. nov., Isolated from the Microbiota of the Planarian Schmidtea mediterranea
Luis Johnson Kangale, Didier Raoult, Fournier Pierre-Edouard
Microbiology Research.2021; 12(2): 268. CrossRef
Flavobacterium phycosphaerae sp. nov. isolated from the phycosphere of Microcystis aeruginosa
Minkyung Kim, Byoung-Hee Lee, Ki-Eun Lee, Woojun Park
International Journal of Systematic and Evolutionary Microbiology .2019;[Epub] CrossRef
Pedobacter vanadiisoli sp. nov., isolated from soil of a vanadium mine
Zhiyong Wang, Yuanqing Tan, Ding Xu, Gejiao Wang, Jihong Yuan, Shixue Zheng
International Journal of Systematic and Evolutionary Microbiology.2016; 66(12): 5112. CrossRef

Research Support, N.I.H., Extramural

Sulfolipid Accumulation in Mycobacterium tuberculosis Disrupted in the mce2 Operon: Olivera Marjanovic , Anthony T. Iavarone , Lee W. Riley; J. Microbiol. 2011;49(3):441-447. Published online June 30, 2011; DOI: https://doi.org/10.1007/s12275-011-0435-4

283 View
0 Download
23 Crossref

Abstract PDF

Mycobacterium tuberculosis, the causative agent of tuberculosis, has a lipid-rich cell wall that serves as an effective barrier against drugs and toxic host cell products, which may contribute to the organism’s persistence in a host. M. tuberculosis contains four homologous operons called mce (mce1-4) that encode putative ABC transporters involved in lipid importation across the cell wall. Here, we analyzed the lipid composition of M. tuberculosis disrupted in the mce2 operon. High resolution mass spectrometric and thin layer chromatographic analyses of the mutant’s cell wall lipid extracts showed accumulation of SL-1 and SL1278 molecules. Radiographic quantitative analysis and densitometry revealed 2.9, 3.9 and 9.8-fold greater amount of [35S] SL-1 in the mce2 operon mutant compared to the wild type M. tuberculosis during the early/mid logarithmic, late logarithmic and stationary phase of growth in liquid broth, respectively. The amount of [35S] SL1278 in the mutant also increased progressively over the same growth phases. The expression of the mce2 operon genes in the wild type strain progressively increased from the logarithmic to the stationary phase of bacterial growth in vitro, which inversely correlated with the proportion of radiolabel incorporation into SL-1 and SL1278 at these phases. Since the mce2 operon is regulated in wild type M. tuberculosis, its cell wall may undergo changes in SL-1 and SL1278 contents during a natural course of infection and this may serve as an important adaptive strategy for M. tuberculosis to maintain persistence in a host.

Citations

Citations to this article as recorded by

Diversification of gene content in the Mycobacterium tuberculosis complex is determined by phylogenetic and ecological signatures
Taiana Tainá Silva-Pereira, Naila Cristina Soler-Camargo, Ana Marcia Sá Guimarães, Se-Ran Jun
Microbiology Spectrum.2024;[Epub] CrossRef
An Update on Mycobacterium Tuberculosis Lipoproteins
María M Bigi, Marina A Forrellad, Julia S García, Federico C Blanco, Cristina L Vázquez, Fabiana Bigi
Future Microbiology.2023; 18(18): 1381. CrossRef
Mce1R of Mycobacterium tuberculosis prefers long-chain fatty acids as specific ligands: a computational study
Dipanwita Maity, Dheeraj Singh, Amitava Bandhu
Molecular Diversity.2023; 27(6): 2523. CrossRef
An In Vivo Model of Separate M. tuberculosis Phagocytosis by Neutrophils and Macrophages: Gene Expression Profiles in the Parasite and Disease Development in the Mouse Host
Elena Kondratieva, Konstantin Majorov, Artem Grigorov, Yulia Skvortsova, Tatiana Kondratieva, Elvira Rubakova, Irina Linge, Tatyana Azhikina, Alexander Apt
International Journal of Molecular Sciences.2022; 23(6): 2961. CrossRef
Mycobacterial MCE proteins as transporters that control lipid homeostasis of the cell wall
Laura I. Klepp, Julia Sabio y Garcia, FabianaBigi
Tuberculosis.2022; 132: 102162. CrossRef
The rate and role of pseudogenes of the Mycobacterium tuberculosis complex
Naila Cristina Soler-Camargo, Taiana Tainá Silva-Pereira, Cristina Kraemer Zimpel, Maurício F. Camacho, André Zelanis, Alexandre H. Aono, José Salvatore Patané, Andrea Pires dos Santos, Ana Marcia Sá Guimarães
Microbial Genomics .2022;[Epub] CrossRef
Role of Ring6 in the Function of the E. coli MCE Protein LetB
Casey Vieni, Nicolas Coudray, Georgia L. Isom, Gira Bhabha, Damian C. Ekiert
Journal of Molecular Biology.2022; 434(7): 167463. CrossRef
Commonalities of Mycobacterium tuberculosis Transcriptomes in Response to Defined Persisting Macrophage Stresses
Catherine Vilchèze, Bo Yan, Rosalyn Casey, Suzie Hingley-Wilson, Laurence Ettwiller, William R. Jacobs
Frontiers in Immunology.2022;[Epub] CrossRef
Structural insights into the substrate-binding proteins Mce1A and Mce4A from Mycobacterium tuberculosis
Pooja Asthana, Dhirendra Singh, Jan Skov Pedersen, Mikko J. Hynönen, Ramita Sulu, Abhinandan V. Murthy, Mikko Laitaoja, Janne Jänis, Lee W. Riley, Rajaram Venkatesan
IUCrJ.2021; 8(5): 757. CrossRef
Transporters Involved in the Biogenesis and Functionalization of the Mycobacterial Cell Envelope
Mary Jackson, Casey M. Stevens, Lei Zhang, Helen I. Zgurskaya, Michael Niederweis
Chemical Reviews.2021; 121(9): 5124. CrossRef
Molecular Cloning, Purification and Characterization of Mce1R of Mycobacterium tuberculosis
Dipanwita Maity, Rajasekhara Reddy Katreddy, Amitava Bandhu
Molecular Biotechnology.2021; 63(3): 200. CrossRef
The Actinobacterial mce Operon: Structure and Functions
M. V. Zaychikova, V. N. Danilenko
Biology Bulletin Reviews.2020; 10(6): 520. CrossRef
Mammalian cell entry operons; novel and major subset candidates for diagnostics with special reference to Mycobacterium avium subspecies paratuberculosis infection
Zahra Hemati, Abdollah Derakhshandeh, Masoud Haghkhah, Kundan Kumar Chaubey, Saurabh Gupta, Manju Singh, Shoorvir V. Singh, Kuldeep Dhama
Veterinary Quarterly.2019; 39(1): 65. CrossRef
Shared Pathogenomic Patterns Characterize a New Phylotype, Revealing Transition toward Host-Adaptation Long before Speciation of Mycobacterium tuberculosis
Guillaume Sapriel, Roland Brosch, Eric Bapteste
Genome Biology and Evolution.2019; 11(8): 2420. CrossRef
MCE domain proteins: conserved inner membrane lipid-binding proteins required for outer membrane homeostasis
Georgia L. Isom, Nathaniel J. Davies, Zhi-Soon Chong, Jack A. Bryant, Mohammed Jamshad, Maria Sharif, Adam F. Cunningham, Timothy J. Knowles, Shu-Sin Chng, Jeffrey A. Cole, Ian R. Henderson
Scientific Reports.2017;[Epub] CrossRef
Molecular and functional analysis of the mce4 operon in Mycobacterium smegmatis
Julia García‐Fernández, Kadamba Papavinasasundaram, Beatriz Galán, Christopher M. Sassetti, José L. García
Environmental Microbiology.2017; 19(9): 3689. CrossRef
Unravelling the pleiotropic role of the MceGATPase in Mycobacterium smegmatis
Julia García‐Fernández, Kadamba Papavinasasundaram, Beatriz Galán, Christopher M. Sassetti, José L. García
Environmental Microbiology.2017; 19(7): 2564. CrossRef
Lipid metabolism in mycobacteria—Insights using mass spectrometry-based lipidomics
Peter J. Crick, Xue Li Guan
Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids.2016; 1861(1): 60. CrossRef
Lipid transport in Mycobacterium tuberculosis and its implications in virulence and drug development
Rebeca Bailo, Apoorva Bhatt, José A. Aínsa
Biochemical Pharmacology.2015; 96(3): 159. CrossRef
Study of the in vivo role of Mce2R, the transcriptional regulator of mce2 operon in Mycobacterium tuberculosis
Marina Andrea Forrellad, María Verónica Bianco, Federico Carlos Blanco, Javier Nuñez, Laura Inés Klepp, Cristina Lourdes Vazquez, María de la Paz Santangelo, Rosana Valeria Rocha, Marcelo Soria, Paul Golby, Maximiliano Gabriel Gutierrez, Fabiana Bigi
BMC Microbiology.2013;[Epub] CrossRef
Total synthesis, stereochemical elucidation and biological evaluation of Ac2SGL; a 1,3-methyl branched sulfoglycolipid from Mycobacterium tuberculosis
Danny Geerdink, Bjorn ter Horst, Marco Lepore, Lucia Mori, Germain Puzo, Anna K. H. Hirsch, Martine Gilleron, Gennaro de Libero, Adriaan J. Minnaard
Chem. Sci..2013; 4(2): 709. CrossRef
Virulence factors of theMycobacterium tuberculosiscomplex
Marina A. Forrellad, Laura I. Klepp, Andrea Gioffré, Julia Sabio y García, Hector R. Morbidoni, María de la Paz Santangelo, Angel A. Cataldi, Fabiana Bigi
Virulence.2013; 4(1): 3. CrossRef
Characterization of Sulfolipids of Mycobacterium tuberculosis H37Rv by Multiple-Stage Linear Ion-Trap High-Resolution Mass Spectrometry with Electrospray Ionization Reveals That the Family of Sulfolipid II Predominates
Elizabeth R. Rhoades, Cassandra Streeter, John Turk, Fong-Fu Hsu
Biochemistry.2011; 50(42): 9135. CrossRef

Research Support, U.S. Gov't, Non-P.H.S.

Transcriptional Control of Genes Involved in Yeast Phospholipid Biosynthesis: Roshini Wimalarathna , Chen-Han Tsai , Chang-Hui Shen; J. Microbiol. 2011;49(2):265-273. Published online May 3, 2011; DOI: https://doi.org/10.1007/s12275-011-1130-1

257 View
0 Download
11 Crossref

Abstract PDF

Phospholipid biosynthetic genes encode enzymes responsible for phospholipid biosynthesis. They are coordinately regulated by the availability of phospholipid precursors through the inositol-sensitive upstream activating sequence (UASINO). However, not all phospholipid genes are UASINO-containing genes and not all UASINO-containing genes have the same response to the phospholipid precursors. Therefore, the transcriptional regulation of phospholipid genes in response to the availability of phospholipid precursors is still unclear. Here, 22 out of 47 phospholipid biosynthetic genes were identified as UASINO-containing genes, including EKI1, EPT1, INM1, IPK2, KCS1, PAH1, and PIK1 which have never been reported before. We also showed, using qRTPCR technique, that 12 UASINO-containing genes are down-regulated by 100 μM inositol in the wild type cells and up-regulated by 100 μM inositol in the ino2Δ cells. Therefore, it is possible that these genes are transcriptionally regulated by the UASINO through the negative response of Ino2p to inositol. One other UASINO-containing gene might be regulated by the positive response of Ino2p to 100 μM inositol. Surprisingly, we found 9 UASINO-containing genes are not dependent on the response of Ino2p to 100 μM inositol, indicating that they may be regulated by other pathway. Furthermore, we identified 9 and 3 non-UASINO-containing genes that are possibly regulated by the negative and positive response of Ino2p to 100 μM inositol, respectively. Therefore, these observations provide insight into the understanding of the co-regulated phospholipid biosynthetic genes expression.

Citations

Citations to this article as recorded by

Identification of a crucial INO2 allele for enhancing ethanol resistance in an industrial fermentation strain of Saccharomyces cerevisiae
Sonia Albillos-Arenal, Javier Alonso del Real, Ana Cristina Adam, María Lairón-Peris, Eladio Barrio, Amparo Querol, Pedro H. Oliveira
mSystems.2025;[Epub] CrossRef
Opi1-mediated transcriptional modulation orchestrates genotoxic stress response in budding yeast
Giovanna Marques Panessa, Eduardo Tassoni-Tsuchida, Marina Rodrigues Pires, Rodrigo Rodrigues Felix, Rafaella Jekabson, Nadja Cristhina de Souza-Pinto, Fernanda Marques da Cunha, Onn Brandman, José Renato Rosa Cussiol, J Surtees
GENETICS.2023;[Epub] CrossRef
Effect of magnesium ions on glucaric acid production in the engineered Saccharomyces cerevisiae
Yunying Zhao, Jie Li, Ruifang Su, Yingli Liu, Jing Wang, Yu Deng
Journal of Biotechnology.2021; 332: 61. CrossRef
Opi1p translocation to the nucleus is regulated by hydrogen peroxide inSaccharomyces cerevisiae
Carolina Camelo, Filipe Vilas-Boas, Andreia Pereira Cepeda, Carla Real, Joana Barros-Martins, Francisco Pinto, Helena Soares, H. Susana Marinho, Luisa Cyrne
Yeast.2017; 34(9): 383. CrossRef
The Transcriptional Activator Ino2p Dissociates from the Yeast INM1 Promoter in Induction
Lingzhi Zhang, Jing Di
DNA and Cell Biology.2014; 33(12): 863. CrossRef
Co-dependent recruitment of Ino80p and Snf2p is required for yeast CUP1 activation
Roshini N. Wimalarathna, Po Yun Pan, Chang-Hui Shen
Biochemistry and Cell Biology.2014; 92(1): 69. CrossRef
Artificial transcription regulator as a tool for improvement of cellular property in Saccharomyces cerevisiae
Sang-Woo Lee, Eunji Kim, Jin-Soo Kim, Min-Kyu Oh
Chemical Engineering Science.2013; 103: 42. CrossRef
Neuroprotective role of taurine during aging
Abdeslem El Idrissi, Chang Hui Shen, William J. L’Amoreaux
Amino Acids.2013; 45(4): 735. CrossRef
Regulation of lipid biosynthesis by phosphatidylinositol‐specific phospholipase C through the transcriptional repression of upstream activating sequence inositol containing genes
Sunny D. Rupwate, Preeti S. Rupwate, Ram Rajasekharan
FEBS Letters.2012; 586(10): 1555. CrossRef
INO1 induction requires chromatin remodelers Ino80p and Snf2p but not the histone acetylases
Paulina Konarzewska, Michelle Esposito, Chang-Hui Shen
Biochemical and Biophysical Research Communications.2012; 418(3): 483. CrossRef
Chromatin repositioning activity and transcription machinery are both recruited by Ace1p in yeast CUP1 activation
Roshini N. Wimalarathna, Po Yun Pan, Chang-Hui Shen
Biochemical and Biophysical Research Communications.2012; 422(4): 658. CrossRef

Journal Article

The Effect of Lipid Supplements on Ruminal Bacteria in Continuous Culture Fermenters Varies with the Fatty Acid Composition: Ramesh B. Potu , Amer A. AbuGhazaleh , Darcie Hastings , Karen Jones , Salam A. Ibrahim; J. Microbiol. 2011;49(2):216-223. Published online May 3, 2011; DOI: https://doi.org/10.1007/s12275-011-0365-1

265 View
0 Download
38 Crossref

Abstract PDF

A single flow continuous culture fermenter system was used in this study to investigate the influence of dietary lipid supplements varying in their fatty acid content on the DNA concentration of selected rumen bacteria. Four continuous culture fermenters were used in a 4×4 Latin square design with four periods of 10 d each. Treatment diets were fed at 45 g/d (DM basis) in three equal portions during the day. The diets were: 1) control (CON), 2) control with animal fat source (SAT), 3) control with soybean oil (SBO), and 4) control with fish oil (FO). Lipid supplements were added at 3% of diet DM. The concentrations of total volatile fatty acids and acetate were not affected (P>0.05) by lipid supplements. Concentrations of propionate, iso-butyrate, valerate and iso-valerate were highest (P<0.05) with the FO diet compared with the other treatment diets. The concentration of t11 C18:1 (vaccenic acid, VA) in effluents increased (P<0.05) with SBO and FO diets and was highest with the SBO diet. The concentrations of C18:0 in effluents were lowest (P<0.05) for the FO diet compared with the other treatment diets. Concentrations of DNA for Anaerovibrio lipolytica, and Butyrivibrio proteoclasticus in fermenters were similar (P>0.05) for all diets. The DNA concentrations of Butyrivibrio fibrisolvens and Ruminococcus albus in fermenters were lowest (P<0.05) with the FO diet but were similar (P>0.05) among the other treatment diets. Selenomonas ruminantium DNA concentration in fermenters was highest (P<0.05) with the FO diet. In conclusion, SBO had no effect on bacterial DNA concentrations tested in this study and the VA accumulation in the rumen observed on the FO diet may be due in part to FO influence on B. fibrisolvens, R. albus, and S. ruminantium.

Citations

Citations to this article as recorded by

Nanoemulsified Corn Oil in Lactating Barki Nutrition: Effect on Intake, Nutrient Digestibility, Rumen Fermentation Characteristics, and Microbial Population
Min Gao, Rong-Qing Li, Mostafa S. A. Khattab, Ahmed M. Abd El Tawab, Yong-Bin Liu, Mohamed El-Sherbiny
Animals.2025; 15(10): 1424. CrossRef
Meta-analysis of in vitro studies on the effects of rumen-protected oils on rumen fermentation products, methane mitigation, microbial populations, and feed digestibility
Hajrian Rizqi Albarki, Muhammad Sulaiman Daulai, Raihani Indah Kusuma, Chanon Suntara, Chuleemas Boonthai Iwai, Anuraga Jayanegara, Anusorn Cherdthong
Animal Feed Science and Technology.2025; 329: 116514. CrossRef
Simulating Precision Feeding of High-Concentrate Diets with High-Fat Inclusion and Different Plant-Based Saturated, Unsaturated, and Animal Fat Sources in Continuous Culture Fermenters
Saad M. Hussein, Thomas C. Jenkins, Matias J. Aguerre, William C. Bridges, Gustavo J. Lascano
Animals.2025; 15(16): 2406. CrossRef
Effects of supplementing tributyrin on serum biochemical indices and meat quality characteristics of longissimus thoracis et lumborum of weaned Small-Tailed Han lambs
Ya-Xin Wang, Xue-Er Wang, Zhi-Wei Li, Ran An, Qing-Chang Ren, Jian-Zhuang Tan
Animal Nutriomics.2024;[Epub] CrossRef
Efficacy of zinc nanoparticle supplementation on ruminal environment in lambs
Daniel Petrič, Klára Mikulová, Alexandra Bombárová, Dominika Batťányi, Klaudia Čobanová, Pavel Kopel, Anna Łukomska, Piotr Pawlak, Pola Sidoruk, Szymon Kotwica, Adam Cieslak, Zora Váradyová
BMC Veterinary Research.2024;[Epub] CrossRef
Effects of Supplementing Tributyrin on Meat Quality Characteristics of Foreshank Muscle of Weaned Small-Tailed Han Sheep Lambs
Xue-Er Wang, Zhi-Wei Li, Li-Lin Liu, Qing-Chang Ren
Animals.2024; 14(8): 1235. CrossRef
Investigating the effect of supplementing different levels of Isochrysis galbana on in vitro rumen fermentation parameters
Zahra Salehian, Hamed Khalilvandi‐Behroozyar, Rasoul Pirmohammadi, Nasrollah Ahmadifard, Hadi Almasi, Mohammad Ramin
Animal Science Journal.2024;[Epub] CrossRef
Does macauba cake alter nutrient digestibility and microorganism population in the rumen of sheep?
Luciana Castro Geraseev, Eduardo Robson Duarte, Edvaldo Alves Vieira, Niurca González Ibarra, Amália Saturnino Chaves, Sarah Silva Santos, Alcinei Mistico Azevedo, Rodrigo Augusto Cortêz Passetti
Tropical Animal Health and Production.2023;[Epub] CrossRef
Effect of Feeding Dried Apple Pomace on Ruminal Fermentation, Methane Emission, and Biohydrogenation of Unsaturated Fatty Acids in Dairy Cows
Khaled Gadulrab, Pola Sidoruk, Martyna Kozłowska, Małgorzata Szumacher-Strabel, Dorota Lechniak, Paweł Kołodziejski, Jarosław Pytlewski, Nina Strzałkowska, Jarosław Olav Horbańczuk, Artur Jóźwik, Yulianri Rizki Yanza, Agung Irawan, Amlan Kumar Patra, Adam
Agriculture.2023; 13(10): 2032. CrossRef
Oil-in-Water Nanoemulsion Can Modulate the Fermentation, Fatty Acid Accumulation, and the Microbial Population in Rumen Batch Cultures
Mohamed El-Sherbiny, Mostafa S. A. Khattab, Ahmed M. Abd El Tawab, Mostafa Elnahr, Adam Cieślak, Małgorzata Szumacher-Strabel
Molecules.2023; 28(1): 358. CrossRef
Milk fatty acid composition, rumen microbial population and animal performance in response to diets rich in linoleic acid supplemented with Piper betle leaves in Saanen goats
R. A. P. Purba, C. Yuangklang, S. Paengkoum, P. Paengkoum
Animal Production Science.2022; 62(14): 1391. CrossRef
Dietary Coleus amboinicus Lour. decreases ruminal methanogenesis and biohydrogenation, and improves meat quality and fatty acid composition in longissimus thoracis muscle of lambs
Yulianri Rizki Yanza, Malgorzata Szumacher-Strabel, Dorota Lechniak, Sylwester Ślusarczyk, Pawel Kolodziejski, Amlan Kumar Patra, Zora Váradyová, Dariusz Lisiak, Mina Vazirigohar, Adam Cieslak
Journal of Animal Science and Biotechnology.2022;[Epub] CrossRef
Effect of Paulownia Leaves Extract Levels on In Vitro Ruminal Fermentation, Microbial Population, Methane Production, and Fatty Acid Biohydrogenation
Bogumiła Nowak, Barbara Moniuszko-Szajwaj, Maria Skorupka, Julia Puchalska, Martyna Kozłowska, Jan Bocianowski, Paweł Antoni Kołodziejski, Małgorzata Szumacher-Strabel, Amlan Kumar Patra, Anna Stochmal, Adam Cieslak
Molecules.2022; 27(13): 4288. CrossRef
Effect of Sainfoin (Onobrychis viciifolia) Pellets on Rumen Microbiome and Histopathology in Lambs Exposed to Gastrointestinal Nematodes
Daniel Petrič, Michaela Komáromyová, Dominika Batťányi, Martyna Kozłowska, Weronika Filipiak, Anna Łukomska, Sylwester Ślusarczyk, Malgorzata Szumacher-Strabel, Adam Cieślak, Marián Várady, Svetlana Kišidayová, Zora Váradyová
Agriculture.2022; 12(2): 301. CrossRef
Effects of heat stress on in vivo and in vitro ruminal metabolism in fat-tailed ewes
A. Amini, R. Pirmohammadi, H. Khalilvandi-Behroozyar, R. Mazaheri-Khameneh, John Gaughan
Animal Production Science.2022; 62(9): 860. CrossRef
Metagenomic Characterization and Volatile Compounds Determination in Rumen from Saanen Goat Kids Fed Olive Leaves
Francesca Bennato, Camillo Martino, Marco Di Domenico, Andrea Ianni, Benli Chai, Lisa Di Marcantonio, Cesare Cammà, Giuseppe Martino
Veterinary Sciences.2022; 9(9): 452. CrossRef
Subgingival Microbiome and Specialized Pro-Resolving Lipid Mediator Pathway Profiles Are Correlated in Periodontal Inflammation
Chun-Teh Lee, Ruoxing Li, Lisha Zhu, Gena D. Tribble, W. Jim Zheng, Brittney Ferguson, Krishna Rao Maddipati, Nikola Angelov, Thomas E. Van Dyke
Frontiers in Immunology.2021;[Epub] CrossRef
Effects of feeding urea-treated triticale and oat grain mixtures on ruminal fermentation, microbial population, and milk production performance of midlactation dairy cows
Kacper Libera, Malgorzata Szumacher-Strabel, Mina Vazirigohar, Wiktor Zieliński, Rafal Lukow, Klaudia Wysocka, Pawel Kołodziejski, Dorota Lechniak, Zora Varadyova, Amlan Kumar Patra, Adam Cieslak
Annals of Animal Science.2021; 21(3): 1007. CrossRef
Potential of walnut (Juglans regia) leave ethanolic extract to modify ruminal fermentation, microbial populations and mitigate methane emission
M. Sahebi Ala, R. Pirmohammadi, H. Khalilvandi-Behroozyar, E. Anassori
Animal Production Science.2020; 60(9): 1189. CrossRef
Ruminal fermentation, microbial population and lipid metabolism in gastrointestinal nematode-infected lambs fed a diet supplemented with herbal mixtures
Paulina Szulc, Dominika Mravčáková, Malgorzata Szumacher-Strabel, Zora Váradyová, Marián Várady, Klaudia Čobanová, Linggawastu Syahrulawal, Amlan Kumar Patra, Adam Cieslak, Simon Russell Clegg
PLOS ONE.2020; 15(4): e0231516. CrossRef
Dietary supplemental plant oils reduce methanogenesis from anaerobic microbial fermentation in the rumen
Julio Ernesto Vargas, Sonia Andrés, Lorena López-Ferreras, Timothy J. Snelling, David R. Yáñez-Ruíz, Carlos García-Estrada, Secundino López
Scientific Reports.2020;[Epub] CrossRef
Rapeseed and linseed oil supplementation affects hydrolytic activities in the rumen of sheep
Małgorzata P. Majewska, Renata Miltko, Agata Krawczyńska, Grzegorz Bełżecki, Barbara Kowalik
Livestock Science.2020; 240: 104175. CrossRef
Influence of grain type and oil concentration of dried corn distillers’ grain with solubles on ruminal fermentation and in vitro gas production in cattle fed high-concentrate diets
Faithe E. Keomanivong, Megan C. Ruch, Mary A. Rodenhuis, Matthew S. Crouse, James D. Kirsch, Marc L. Bauer, Md S. Borhan, Shafiqur Rahman, Kendall C. Swanson, J. Plaizier
Canadian Journal of Animal Science.2019; 99(1): 160. CrossRef
Effects of dairy cow diets supplied with flaxseed oil and propolis extract, with or without vitamin E, on the ruminal microbiota, biohydrogenation, and digestion
E.H. Yoshimura, N.W. Santos, E. Machado, B.C. Agustinho, L.M. Pereira, S.C. de Aguiar, R. Franzolin, E. Gasparino, G.T. dos Santos, L.M. Zeoula
Animal Feed Science and Technology.2018; 241: 163. CrossRef
Effect of soya bean oil supplementation and forage type on methane production and fibre digestibility using the in vitro gas production system
J. P. P. Rodrigues, M. Ramin, P. Huhtanen, F. Aru, E. Detmann, M. I. Marcondes
Grass and Forage Science.2018; 73(2): 368. CrossRef
Effect of Sunflower and Marine Oils on Ruminal Microbiota, In vitro Fermentation and Digesta Fatty Acid Profile
Julio E. Vargas, Sonia Andrés, Timothy J. Snelling, Lorena López-Ferreras, David R. Yáñez-Ruíz, Carlos García-Estrada, Secundino López
Frontiers in Microbiology.2017;[Epub] CrossRef
The effects of the unsaturated degree of long-chain fatty acids on the rumen microbial protein content and the activities of transaminases and dehydrogenase in vitro
Jian GAO, Yu-jia JING, Meng-zhi WANG, Liang-feng SHI, Shi-min LIU
Journal of Integrative Agriculture.2016; 15(2): 424. CrossRef
Impacts of the unsaturation degree of long-chain fatty acids on the volatile fatty acid profiles of rumen microbial fermentation in goats in vitro
Jian GAO, Meng-zhi WANG, Yu-jia JING, Xue-zhao SUN, Tian-yi WU, Liang-feng SHI
Journal of Integrative Agriculture.2016; 15(12): 2827. CrossRef
Quantitative analysis of ruminal bacterial populations involved in lipid metabolism in dairy cows fed different vegetable oils
E. Vargas-Bello-Pérez, N. Cancino-Padilla, J. Romero, P.C. Garnsworthy
Animal.2016; 10(11): 1821. CrossRef
Rumen fermentation, methane concentration and fatty acid proportion in the rumen and milk of dairy cows fed condensed tannin and/or fish-soybean oils blend
J. Szczechowiak, M. Szumacher-Strabel, M. El-Sherbiny, E. Pers-Kamczyc, P. Pawlak, A. Cieslak
Animal Feed Science and Technology.2016; 216: 93. CrossRef
The effects of cinnamaldehyde, monensin and quebracho condensed tannin on rumen fermentation, biohydrogenation and bacteria in continuous culture system
A. Ishlak, M. Günal, A.A. AbuGhazaleh
Animal Feed Science and Technology.2015; 207: 31. CrossRef
Tomato seeds as a novel by-product feed for lactating dairy cows
C.A. Cassinerio, J.G. Fadel, J. Asmus, J.M. Heguy, S.J. Taylor, E.J. DePeters
Journal of Dairy Science.2015; 98(7): 4811. CrossRef
Effects of incremental amounts of fish oil on trans fatty acids and Butyrivibrio bacteria in continuous culture fermenters
A. A. AbuGhazaleh, A. Ishlak
Journal of Animal Physiology and Animal Nutrition.2014; 98(2): 271. CrossRef
Short communication: Effect of blackberry and pomegranate oils on vaccenic acid formation in a single-flow continuous culture fermentation system
A. Ishlak, A.A. AbuGhazaleh, M. Günal
Journal of Dairy Science.2014; 97(2): 1067. CrossRef
Garlic derived compounds modify ruminal fatty acid biohydrogenation and induce shifts in the Butyrivibrio community in continuous-culture fermenters
E. Ramos-Morales, G. Martínez-Fernández, L. Abecia, A.I. Martin-García, E. Molina-Alcaide, D.R. Yáñez-Ruiz
Animal Feed Science and Technology.2013; 184(1-4): 38. CrossRef
Dietary Fish Oil Supplements Modify Ruminal Biohydrogenation, Alter the Flow of Fatty Acids at the Omasum, and Induce Changes in the Ruminal Butyrivibrio Population in Lactating Cows
Kevin J. Shingfield, Piia Kairenius, Anu Ärölä, Delphine Paillard, Stefan Muetzel, Seppo Ahvenjärvi, Aila Vanhatalo, Pekka Huhtanen, Vesa Toivonen, J. Mikko Griinari, R. John Wallace
The Journal of Nutrition.2012; 142(8): 1437. CrossRef
The effect of level of forage and oil supplement on biohydrogenation intermediates and bacteria in continuous cultures
P. Gudla, A.A. AbuGhazaleh, A. Ishlak, K. Jones
Animal Feed Science and Technology.2012; 171(2-4): 108. CrossRef
The Effect of Forage Level and Oil Supplement on Butyrivibrio fibrisolvens and Anaerovibrio lipolytica in Continuous Culture Fermenters
P. Gudla, A. Ishlak, A. A. AbuGhazaleh
Asian-Australasian Journal of Animal Sciences.2011; 25(2): 234. CrossRef

Research Support, Non-U.S. Gov't

Novel Pathogenetic Mechanism in a Clinical Isolate of Yersinia enterocolitica KU14: Yoshinori Sato , Kenichi Kaneko , Takeshi Sasahara , Matsuhisa Inoue; J. Microbiol. 2006;44(1):98-105.; DOI: https://doi.org/2330 [pii]

213 View
0 Download

Abstract PDF: Yersinia enterocolitica induces a broad range of gastrointestinal syndromes, including acute enteritis. We previously reported that the clinical isolate, Y. enterocolitica KU14, which lacks pYV, was still capable of causing clinical infection. The present study demonstrated that KU14 did not trigger the death of macrophages in vitro, unlike WA-314 (ATCC51871, which harbors the pYV virulence plasmid). However, the intracellular growth of KU14 in the macrophages was greater than that of WA-C (ATCC51872, a non-plasmid harboring the derivative pYV plasmid). Treatment with a cholesterol-binding drug (β-cyclodextrin) that affected lipid rafts resulted in a dramatic reduction in the intracellular growth of KU14. These data clearly indicate that the enhanced intracellular growth of KU14 is related to lipid raft-mediated infection.

Adsorption of Pb^2+ in the components of bacterial cell membrane: Kim , Mal Nam; J. Microbiol. 1995;33(4):278-282.

215 View
0 Download

Abstract PDF: S. epidermidis cell was fractionated into cell wall, cell membrane and cytoplasm. The cell membrane adsorbed the most abundant Pb^2+ per unit dry weight of the three fractions tested. Adsorption behavior of Pb^2+ in lipid and protein, which are the main components of the cell membrane, indicated that phosphatidylethanolamine and phosphatidylinositol having phosphoryl group and gangliosides containing carboxyl groups adsorbed much more Pb^2+ than triglycerides lacking any chargeable functional groups. Protein purified from cell membrane adsorbed larger amount of Pb^2+ than total native cell membrane or cell membrane lipid.

First
Prev
Page of 1
Next
Last

Search