Research Support, Non-U.S. Gov't
- Acinetobacter baumannii Outer Membrane Protein A Modulates the Biogenesis of Outer Membrane Vesicles
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Dong Chan Moon , Chul Hee Choi , Jung Hwa Lee , Chi-Won Choi , Hye-Yeon Kim , Jeong Soon Park , Seung Il Kim , Je Chul Lee
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J. Microbiol. 2012;50(1):155-160. Published online February 27, 2012
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DOI: https://doi.org/10.1007/s12275-012-1589-4
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Abstract
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Acinetobacter baumannii secretes outer membrane vesicles
(OMVs) during both in vitro and in vivo growth, but the
biogenesis mechanism by which A. baumannii produces
OMVs remains undefined. Outer membrane protein A of
A. baumannii (AbOmpA) is a major protein in the outer
membrane and the C-terminus of AbOmpA interacts with
diaminopimelate of peptidoglycan. This study investigated
the role of AbOmpA in the biogenesis of A. baumannii
OMVs. Quantitative and qualitative approaches were used
to analyze OMV biogenesis in A. baumannii ATCC 19606T
and an isogenic ΔAbOmpA mutant. OMV production was
significantly increased in the ΔAbOmpA mutant compared
to wild-type bacteria as demonstrated by quantitation of
proteins and lipopolysaccharides (LPS) packaged in OMVs.
LPS profiles prepared from OMVs from wild-type bacteria
and the ΔAbOmpA mutant had identical patterns, but
proteomic analysis showed different protein constituents in
OMVs from wild-type bacteria compared to the ΔAbOmpA
mutant. In conclusion, AbOmpA influences OMV biogenesis
by controlling OMV production and protein composition.
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