Journal of Microbiology

Isolation of Synthetic Lethal Mutations in the rsm1-null Mutant of Fission Yeast: DongGeRaMi Moon , Yun-Sun Park , Cha-Yeon Kim , Jin Ho Yoon; J. Microbiol. 2010;48(5):701-705. Published online November 3, 2010; DOI: https://doi.org/10.1007/s12275-010-0353-x

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Abstract: To identify mutations in genes that are genetically linked to rsm1, we performed a synthetic lethal genetic screen in the fission yeast, Schizosaccharomyces pombe. Four mutations that showed synthetic lethality in combination with the rsm1null allele were isolated from approximately 320,000 colonies and defined in three complementation groups. One mutant (SLrsm1) exhibited a significant accumulation of poly(A)+ RNA in the nucleus under synthetic lethal conditions, while the rest had no mRNA export defects. In addition, some genes (spmex67, rae1, or mlo3) required for mRNA export complemented the growth defects of the identified mutants. These results suggest that the isolated mutants contain mutations in genes that are involved in mRNA export and/or pre-mRNA retention.

Nup211, the Fission Yeast Homolog of Mlp1/Tpr, Is Involved in mRNA Export: Jin-Ah Bae , DongGeRaMi Moon , Jin Ho Yoon; J. Microbiol. 2009;47(3):337-343. Published online June 26, 2009; DOI: https://doi.org/10.1007/s12275-009-0125-7

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Synthetic lethal mutants have been previously isolated in fission yeast Schizosaccharomyces pombe, which genetically interact with spmex67, in order to identify the genes involved in mRNA export. The nup211 gene was isolated by complementation of the growth defect in one of the synthetic lethal mutants, SLMex2, under synthetic lethal condition. We showed that Nup211, fission yeast homolog of Mlp1/Mlp2/Tpr, is essential for vegetative growth and Nup211-GFP proteins expressed at endogenous level are localized mainly in nuclear periphery. The accumulation of poly(A)+ RNA in the nucleus is exhibited when expression of nup211 is repressed or over-expressed. These results suggest that the Nup211 protein plays a pivotal role of mRNA export in fission yeast.

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Fission yeast essential nuclear pore protein Nup211 regulates the expression of genes involved in cytokinesis
Domenick Kamel, Ayisha Sookdeo, Ayana Ikenouchi, Hualin Zhong, Juan Mata
PLOS ONE.2024; 19(12): e0312095. CrossRef
Quantitative analysis of nuclear pore complex organization in Schizosaccharomyces pombe
Joseph M Varberg, Jay R Unruh, Andrew J Bestul, Azqa A Khan, Sue L Jaspersen
Life Science Alliance.2022; 5(7): e202201423. CrossRef
Asymmetrical localization of Nup107-160 subcomplex components within the nuclear pore complex in fission yeast
Haruhiko Asakawa, Tomoko Kojidani, Hui-Ju Yang, Chizuru Ohtsuki, Hiroko Osakada, Atsushi Matsuda, Masaaki Iwamoto, Yuji Chikashige, Koji Nagao, Chikashi Obuse, Yasushi Hiraoka, Tokuko Haraguchi, Gregory P. Copenhaver
PLOS Genetics.2019; 15(6): e1008061. CrossRef
The fission yeast nucleoporin Alm1 is required for proteasomal degradation of kinetochore components
Silvia Salas-Pino, Paola Gallardo, Ramón R. Barrales, Sigurd Braun, Rafael R. Daga
Journal of Cell Biology.2017; 216(11): 3591. CrossRef
Tts1, the fission yeast homologue of the TMEM33 family, functions in NE remodeling during mitosis
Dan Zhang, Snezhana Oliferenko, Daniel J. Lew
Molecular Biology of the Cell.2014; 25(19): 2970. CrossRef
Characterization of nuclear pore complex components in fission yeastSchizosaccharomyces pombe
Haruhiko Asakawa, Hui-Ju Yang, Takaharu G Yamamoto, Chizuru Ohtsuki, Yuji Chikashige, Kumiko Sakata-Sogawa, Makio Tokunaga, Masaaki Iwamoto, Yasushi Hiraoka, Tokuko Haraguchi
Nucleus.2014; 5(2): 149. CrossRef
Isolation of synthetic lethal mutations in combination with spnab2 of fission yeast
Yun-Sun Park, Jin Ho Yoon
Genes & Genomics.2012; 34(3): 275. CrossRef
Double duty for nuclear proteins – the price of more open forms of mitosis
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Trends in Genetics.2009; 25(12): 545. CrossRef

The Fission Yeast Homologue of Gle1 is Essential for Growth and Involved in mRNA Export: DongGeRaMi Moon , Jin-Ah Bae , Hyun Jin Cho , Jin Ho Yoon; J. Microbiol. 2008;46(4):422-428. Published online August 31, 2008; DOI: https://doi.org/10.1007/s12275-008-0177-0

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Abstract: We have isolated Gle1 homologue (named as spgle1) as a partial multicopy suppressor of the synthetic lethality of rae1-167 elf1-21 in fission yeast Schizosaccharomyces pombe. The spgle1 is also able to complement partially temperature-sensitive phenotype of rae1-167 only at a lower restrictive temperature. The spgle1 gene contains one intron and encodes a 480 amino-acid protein with predicted molecular weight of 56.2 kDa. We showed that spgle1 gene is essential for vegetative growth and functional Gle1-GFP protein is localized mainly in NPC. The accumulation of poly(A)+ RNA in the nucleus is exhibited when expression of spgle1 is repressed or over-expressed. These results suggest that the spGle1 protein is also involved in mRNA export in fission yeast.

Schizosaccharomyces pombe nup97, which Genetically Interacts with mex67, is Essential for Growth and Involved in mRNA Export: Hyun Jin Cho , Duk Kyung Hwang , Sun Im Jung , Jin Ho Yoon; J. Microbiol. 2007;45(4):344-349.; DOI: https://doi.org/2562 [pii]

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Abstract: We have isolated previously three synthetic lethal mutants in Schizosaccharomyces pombe, which genetically interact with mex67, in order to identify the genes involved in mRNA export. A novel nup97 gene was isolated by complementation of the growth defect in one of the synthetic lethal mutants, SLMex3. The nup97 gene contains one intron and encodes an 851 amino-acid protein that is similar to nucleoporins, Npp106p in S. pombe and Nic96p in Saccharomyces cerevisiae. The nup97 gene is essential for vegetative growth, and nup97 null mutant harboring pREP41X-Nup97 showed poly(A)+ RNA export defect when expression of nup97 is repressed in the presence of thiamine. These results suggest that nup97 is involved in mRNA export from the nucleus to cytoplasm.

Schizosaccharomyces pombe rsm1 Genetically Interacts with spmex67, Which Is Involved in mRNA Export: Jin Ho Yoon; J. Microbiol. 2004;42(1):32-36.; DOI: https://doi.org/2004 [pii]

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Abstract: We have previously isolated three synthetic lethal mutants from Schizosaccharomyces pombe in order to identify mutations in the genes that are functionally linked to spmex67 with respect to mRNA export. A novel rsm1 gene was isolated by complementation of the growth defect in one of the synthetic lethal mutants, SLMex1. The rsm1 gene contains no introns and encodes a 296 amino-acid-long protein with the RING finger domain, a C3HC4 in the N-terminal half. The [delta]rsm1 null mutant is viable, but it showed a slight poly(A)^+ RNA accumulation in the nucleus. Also, the combination of [delta]rsm1 and [delta]spmex67 mutations confers synthetic lethality that is accompanied by the severe poly(A)^+ RNA export defect. These results suggest that rsm1 is involved in mRNA export from the nucleus.

Synthetic Lethal Mutations with spmex67 of Schizosaccharomyces pombe in the Mediation of mRNA Export: Jin Ho Yoon; J. Microbiol. 2003;41(2):115-120.

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Abstract: Mex67p/Tap are evolutionally conserved mRNA export factors. To identify mutations in genes that are functionally linked to mex67 with respect to mRNA export, we used a synthetic lethal genetic screen in Schizosaccharomyces pombe. Three synthetic lethal mutants were isolated and mutations in these mutants defined separate complementation groups. These mutants exhibited the accumulation of poly(A)^+ RNA in the nucleus, with a decrease in the cytoplasm under synthetically lethal conditions, suggesting that the mutations cause an mRNA nuclear export defect. In addition, the S. pombe genes that were found to be involved in mRNA export did not suppress the synthetic lethality of these mutants. These results indicate that the isolated mutants contain mutations in new genes, which are involved in mRNA export from the nucleus.

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