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- Genomic Characterization and Comparative Analysis of Streptococcus zhangguiae sp. nov. Isolated from the Respiratory Tract of Marmota Himalayana
- Caixin Yang, Jiajia Ma, Huimin Zhou, Jing Yang, Ji Pu, Shan Lu, Dong Jin, Liyun Liu, Kui Dong, Jianguo Xu
- J. Microbiol. 2024;62(11):951-963. Published online November 4, 2024
- DOI: https://doi.org/10.1007/s12275-024-00177-2
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Abstract
- Two Gram-stain-positive, oxidase-negative, non-motile, facultative anaerobic, α-hemolytic, coccus-shaped bacteria (zg-86T and zg-70) were isolated from the respiratory tracts of marmots (Marmota Himalayana) on the Qinghai-Tibet Plateau of China. Phylogenetic analysis of the 16S rRNA gene and 545 core genes revealed that these two strains belong to the Streptococcus genus. These strains were most closely related to Streptococcus respiraculi HTS25T, Streptococcus cuniculi CCUG 65085T, and Streptococcus marmotae HTS5T. The average nucleotide identity (ANI) and digital DNA‒DNA hybridization (dDDH) were below the threshold for species delineation. The predominant cellular fatty acids (CFAs) in this novel species were C16:0, C18:0, and C18:1ω9c, whereas the primary polar lipids were phosphatidylglycerol (PG), phosphatidylethanolamine (PE) and an unknown phosphoglycolipid (PGL). The optimal growth conditions for the strains were 37 °C, pH 7.0, and 0.5% (w/v) NaCl on brain-heart infusion (BHI) agar supplemented with 5% defibrinated sheep blood. Comparative genomics analyses revealed the potential pathogenicity of strain zg-86T through comparisons with suis subclade strains in terms of virulence factors, pathogen-host interactions (PHIs) and mobile genetic factors (MGEs). Based on the phenotypic characteristics and phylogenetic analyses, we propose that these two isolates represent novel species in the genus Streptococcus, for which the names Streptococcus zhangguiae sp. nov. (the type strain zg-86T=GDMCC 1.1758T=JCM 34273T) is proposed.
- Complete genetic dissection and cell type-specific replication of old world alphaviruses, getah virus (GETV) and sagiyama virus (SAGV)
- Yiwen Zhang , Jinhan Yu , Lu Tan , Xingxing Wang , Runsheng Li , Dal Young Kim
- J. Microbiol. 2021;59(11):1044-1055. Published online September 27, 2021
- DOI: https://doi.org/10.1007/s12275-021-1361-8
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- 4 Web of Science
- 3 Crossref
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Abstract
- Getah virus (GETV), which was first isolated in Malaysia in 1955, and Sagiyama virus (SAGV), isolated in Japan in 1956, are members of the genus Alphavirus in the family Togaviridae. It is a consensus view that SAGV is a variant of GETV. In the present study, we determined the complete sequences of the prototype GETV MM2021 and SAGV M6-Mag132 genomic RNA extracted from plaque-purified viruses. The MM2021 genome was 11,692 nucleotides (nt) in length in the absence of 3poly(A) tail, and the length of M6-Mag132 genome was 11,698 nt. Through sequence alignment of MM2021 and M6-Mag132, we located all the amino acid differences between these two strains, which were scattered in all the encoded proteins. Subsequently, we validated the close evolutionary relationship between GETV and SAGV by constructing phylogenetic trees based on either complete genomes or structural genomes. We eventually analyzed the growth kinetics of GETV and SAGV as well as other representative alphaviruses in various mammalian and insect cell lines. It was shown that human-oriented cell lines such as HEK-293T and Hela cells were relatively resistant to GETV and SAGV infection due to absence of proviral factors or species-specific barrier. On the other hand, both GETV and SAGV replicated efficiently in non-human cell lines. Our results provide essential genetic information for future epidemiological surveillance on Alphaviruses and lay the foundation for developing effective interventions against GETV and SAGV.
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- Novel Porcine Getah Virus from Diarrheal Piglets in Jiangxi Province, China: Prevalence, Genome Sequence, and Pathogenicity
Jianhui Lan, Mengtao Fang, Leilei Duan, Zhong Liu, Guanggao Wang, Qi Wu, Ke Fan, Dongyan Huang, Yu Ye, Gen Wan, Yuxin Tang, Deping Song
Animals.2024; 14(20): 2980. CrossRef - Characterization and arbovirus susceptibility of cultured CERNI cells derived from sika deer (Cervus nippon)
Makoto Takeishi, Shigeru Morikawa, Ryusei Kuwata, Mitsumori Kawaminami, Hiroshi Shimoda, Haruhiko Isawa, Ken Maeda, Yasuhiro Yoshikawa
In Vitro Cellular & Developmental Biology - Animal.2024; 60(8): 935. CrossRef - Getah Virus Infection Rapidly Causes Testicular Damage and Decreases Sperm Quality in Male Mice
Fengqing Li, Bing Zhang, Zhiwen Xu, Chaoyuan Jiang, Mincai Nei, Lei Xu, Jun Zhao, Huidan Deng, Xiangang Sun, Yuancheng Zhou, Ling Zhu
Frontiers in Veterinary Science.2022;[Epub] CrossRef
- Novel Porcine Getah Virus from Diarrheal Piglets in Jiangxi Province, China: Prevalence, Genome Sequence, and Pathogenicity
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