Journal of Microbiology

Review

Progress and challenges in CRISPR/Cas applications in microalgae: Quynh-Giao Tran, Trang Thi Le, Dong-Yun Choi, Dae-Hyun Cho, Jin-Ho Yun, Hong Il Choi, Hee-Sik Kim, Yong Jae Lee; J. Microbiol. 2025;63(3):e2501028. Published online March 28, 2025; DOI: https://doi.org/10.71150/jm.2501028

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Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) technologies have emerged as powerful tools for precise genome editing, leading to a revolution in genetic research and biotechnology across diverse organisms including microalgae. Since the 1950s, microalgal production has evolved from initial cultivation under controlled conditions to advanced metabolic engineering to meet industrial demands. However, effective genetic modification in microalgae has faced significant challenges, including issues with transformation efficiency, limited target selection, and genetic differences between species, as interspecies genetic variation limits the use of genetic tools from one species to another. This review summarized recent advancements in CRISPR systems applied to microalgae, with a focus on improving gene editing precision and efficiency, while addressing organism-specific challenges. We also discuss notable successes in utilizing the class 2 CRISPR-associated (Cas) proteins, including Cas9 and Cas12a, as well as emerging CRISPR-based approaches tailored to overcome microalgal cellular barriers. Additionally, we propose future perspectives for utilizing CRISPR/Cas strategies in microalgal biotechnology.

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Advancing microbial engineering through synthetic biology
Ki Jun Jeong
Journal of Microbiology.2025; 63(3): e2503100. CrossRef

Journal Article

Application of high-salinity stress for enhancing the lipid productivity of Chlorella sorokiniana HS1 in a two-phase process: Ramesh Kakarla , Jung-Woon Choi , Jin-Ho Yun , Byung-Hyuk Kim , Jina Heo , Sujin Lee , Dae-Hyun Cho , Rishiram Ramanan , Hee-Sik Kim; J. Microbiol. 2018;56(1):56-64. Published online January 4, 2018; DOI: https://doi.org/10.1007/s12275-018-7488-6

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Abstract

Increased lipid accumulation of algal cells as a response to environmental stress factors attracted much attention of researchers to incorporate this stress response into industrial algal cultivation process with the aim of enhancing algal lipid productivity. This study applies high-salinity stress condition to a two-phase process in which microalgal cells are initially grown in freshwater medium until late exponential phase and subsequently subjected to high-salinity condition that induces excessive lipid accumulation. Our initial experiment revealed that the concentrated culture of Chlorella sorokiniana HS1 exhibited the intense fluorescence of Nile red at the NaCl concentration of 60 g/L along with 1 g/L of supplemental bicarbonate after 48 h of induction period without significantly compromising cultural integrity. These conditions were further verified with the algal culture grown for 7 days in a 1 L bottle reactor that reached late exponential phase; a 12% increment in the lipid content of harvested biomass was observed upon inducing high lipid accumulation in the concentrated algal culture at the density of 5.0 g DW/L. Although an increase in the sum of carbohydrate and lipid contents of harvested biomass indicated that the external carbon source supplemented during the induction period increased overall carbon assimilation, a decrease in carbohydrate content suggested the potential reallocation of cellular carbon that promoted lipid droplet formation under high-salinity stress. These
results
thus emphasize that the two-phase process can be successfully implemented to enhance algal lipid productivity by incorporating high-salinity stress conditions into the pre-concentrated sedimentation ponds of industrial algal production system.

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Bioremediation of dissolved organic compounds in produced water from oil and gas operations using Chlorella sorokiniana: a sustainable approach
Mohammed Omar Faruque, Tariq Mohammed, Mohammad Mozahar Hossain, Shaikh Abdur Razzak
Environmental Monitoring and Assessment.2024;[Epub] CrossRef
Optimization of iron, phosphate, and salinity in nutrient medium using response surface methodology for enhancing biochemical composition in Chlorella sp. culture
Jurmin H. Sarri, Mahmut Elp
Algal Research.2024; 84: 103747. CrossRef
Long-term acclimation to organic carbon enhances the production of loliolide from Scenedesmus deserticola
Dae-Hyun Cho, Jin-Ho Yun, Dong-Yoon Choi, Jina Heo, Eun Kyung Kim, Juran Ha, Chan Yoo, Hong Il Choi, Yong Jae Lee, Hee-Sik Kim
Bioresource Technology.2024; 412: 131408. CrossRef
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Motasem Y. D. Alazaiza, Ahmed Albahnasawi, Murat Eyvaz, Tahra Al Maskari, Dia Eddin Nassani, Salem S. Abu Amr, Mohammed Shadi S. Abujazar, Mohammed J. K. Bashir
Energies.2023; 16(2): 791. CrossRef
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Syafiqah Md Nadzir, Norjan Yusof, Norazela Nordin, Azlan Kamari, Mohd Zulkhairi Mohd Yusoff
Biofuels.2023; 14(9): 979. CrossRef
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Anil Kumar Patel, Akash Pralhad Vadrale, Reeta-Rani Singhania, Chiu-Wen Chen, Jo Shu Chang, Cheng-Di Dong
Bioresource Technology.2023; 386: 129477. CrossRef
Potential of Halophytes as Sustainable Fodder Production by Using Saline Resources: A Review of Current Knowledge and Future Directions
Maria Hasnain, Zainul Abideen, Faraz Ali, Mirza Hasanuzzaman, Ali El-Keblawy
Plants.2023; 12(11): 2150. CrossRef
Salinity stress provokes diverse physiological responses of eukaryotic unicellular microalgae
Attila Farkas, Bernadett Pap, Ottó Zsíros, Roland Patai, Prateek Shetty, Győző Garab, Tibor Bíró, Vince Ördög, Gergely Maróti
Algal Research.2023; 73: 103155. CrossRef
Utilization of Saline Water Enhances Lipid Accumulation in Green Microalgae for the Sustainable Production of Biodiesel
Maria Hasnain, Zainul Abideen, Daniel Anthony Dias, Shagufta Naz, Neelma Munir
BioEnergy Research.2023; 16(2): 1026. CrossRef
Cultivation of Freshwater Microalgae in Wastewater Under High Salinity for Biomass, Nutrients Removal, and Fatty Acids/Biodiesel Production
Qi Yang, Min Zhang, Hend A. Alwathnani, Muhammad Usman, Badr A. Mohamed, Abd El-Fatah Abomohra, El-Sayed Salama
Waste and Biomass Valorization.2022; 13(7): 3245. CrossRef
Overview on stress-induced strategies for enhanced microalgae lipid production: Application, mechanisms and challenges
Xueting Song, Bing-Feng Liu, Fanying Kong, Nan-Qi Ren, Hong-Yu Ren
Resources, Conservation and Recycling.2022; 183: 106355. CrossRef
Biodesalination of brackish water coupled with lipid production using native Scenedesmus sp. isolated from a saline lake in Ethiopia, Lake Basaka
Muluwork Maru, Feleke Zewge, Demeke Kifle, Endalkachew Sahle-Demissie
Desalination and Water Treatment.2022; 266: 39. CrossRef
Anaerobic digestates grown oleaginous microalgae for pollutants removal and lipids production
Xiao-Bo Tan, Ya-Lei Zhang, Xian-Chao Zhao, Li-Bin Yang, Shun-Cheng Yangwang, Yue Zou, Jue-Ming Lu
Chemosphere.2022; 308: 136177. CrossRef
A critical review on employing algae as a feed for polycarbohydrate synthesis
V S Lisha, Rushikesh S. Kothale, Sumati Sidharth, Balasubramanian Kandasubramanian
Carbohydrate Polymer Technologies and Applications.2022; 4: 100242. CrossRef
Salinity induces unique changes in lipid classes and fatty acids of the estuarine haptophyte Diacronema vlkianum
José-Pedro Cañavate, Catalina Fernández-Díaz
European Journal of Phycology.2022; 57(3): 297. CrossRef
Algal biopolymers as sustainable resources for a net-zero carbon bioeconomy
A. Aswathi Mohan, Aiswarya Robert Antony, Kozhumal Greeshma, Jin-Ho Yun, Rishiram Ramanan, Hee-Sik Kim
Bioresource Technology.2022; 344: 126397. CrossRef
An appraisal of the variable response of microalgal lipids to culture salinity
José‐Pedro Cañavate, Catalina Fernández‐Díaz
Reviews in Aquaculture.2022; 14(1): 192. CrossRef
Two-stage cultivation of Chlorella vulgaris using light and salt stress conditions for simultaneous production of lipid, carotenoids, and antioxidants
Hamdy Elsayed Ahmed Ali, Eman A. El-fayoumy, Wessam E. Rasmy, Ramadan M. Soliman, Mohd Azmuddin Abdullah
Journal of Applied Phycology.2021; 33(1): 227. CrossRef
Evaluation of the operational conditions in the production and morphology of Chlorella sp.
L. Rendón-Castrillón, M. Ramírez-Carmona, C. Ocampo-López, R. Giraldo-Aristizabal
Brazilian Journal of Biology.2021; 81(1): 202. CrossRef
Historical occurrences of marine microalgal blooms in Indian peninsula: Probable causes and implications
Oyeshina Gideon Oyeku, Subir Kumar Mandal
Oceanologia.2021; 63(1): 51. CrossRef
Sodium chloride and nitrogen effects on Chlorella vulgaris growth and biocommodities production
Styliani E. Biliani, Ioannis D. Manariotis
Desalination and Water Treatment.2021; 237: 159. CrossRef
Two-stage cultivation of microalgae for production of high-value compounds and biofuels: A review
Vinoj Chamilka Liyanaarachchi, Malith Premaratne, Thilini U. Ariyadasa, P.H.V. Nimarshana, Anushree Malik
Algal Research.2021; 57: 102353. CrossRef
The effect on single and combined stresses for biomass and lipid production from Nannochloris atomus using two phase culture system
So Hee Kim, Clovis Awah Che, Gwi-Taek Jeong, Sung-Koo Kim
Journal of Biotechnology.2021; 326: 40. CrossRef
Perspective Design of Algae Photobioreactor for Greenhouses—A Comparative Study
Kateřina Sukačová, Pavel Lošák, Vladimír Brummer, Vítězslav Máša, Daniel Vícha, Tomáš Zavřel
Energies.2021; 14(5): 1338. CrossRef
Development of mutant microalgae that accumulate lipids under nitrate-replete conditions
Tomoki Oyama, Yuichi Kato, Katsuya Satoh, Yutaka Oono, Mami Matsuda, Tomohisa Hasunuma, Akihiko Kondo
Algal Research.2021; 60: 102544. CrossRef
Salinity-induced microalgal-based mariculture wastewater treatment combined with biodiesel production
Chaofan Zhang, Tomohisa Hasunuma, Su Shiung Lam, Akihiko Kondo, Shih-Hsin Ho
Bioresource Technology.2021; 340: 125638. CrossRef
Cultivation and biochemical characterization of isolated Sicilian microalgal species in salt and temperature stress conditions
Rosaria Arena, Serena Lima, Valeria Villanova, Nadia Moukri, Eleonora Curcuraci, Concetta Messina, Andrea Santulli, Francesca Scargiali
Algal Research.2021; 59: 102430. CrossRef
Transcriptomic analysis of Chlorella sp. HS2 suggests the overflow of acetyl‐CoA and NADPH cofactor induces high lipid accumulation and halotolerance
Jin‐Ho Yun, Michaël Pierrelée, Dae‐Hyun Cho, Urim Kim, Jina Heo, Dong‐Yun Choi, Yong Jae Lee, Bongsoo Lee, HyeRan Kim, Bianca Habermann, Yong Keun Chang, Hee‐Sik Kim
Food and Energy Security.2021;[Epub] CrossRef
Utilization of the acid hydrolysate of defatted Chlorella biomass as a sole fermentation substrate for the production of biosurfactant from Bacillus subtilis C9
Jin-Ho Yun, Dae-Hyun Cho, Bongsoo Lee, Yong Jae Lee, Dong-Yun Choi, Hee-Sik Kim, Yong Keun Chang
Algal Research.2020; 47: 101868. CrossRef
Two-stage cultivation strategy for simultaneous increases in growth rate and lipid content of microalgae: A review
Md Maniruzzaman A. Aziz, Khairul Anuar Kassim, Zahra Shokravi, Fauzan Mohd Jakarni, Hong Yuan Liu, Nabilah Zaini, Lian See Tan, A.B.M. Saiful Islam, Hoofar Shokravi
Renewable and Sustainable Energy Reviews.2020; 119: 109621. CrossRef
Exploring the distinctiveness of biomass and biomolecules from limnic microalgae of unexplored waters of Noyyal River, Western Ghats, for exploitation
Pandian Sureshkumar, Jibu Thomas
Environmental Science and Pollution Research.2020; 27(18): 23309. CrossRef
Enhanced lipid productivity of Chlamydomonas reinhardtii with combination of NaCl and CaCl2 stresses
Le Thai Hang, Kazuhiro Mori, Yasuhiro Tanaka, Masaaki Morikawa, Tadashi Toyama
Bioprocess and Biosystems Engineering.2020; 43(6): 971. CrossRef
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Quynh-Giao Tran, Hyang Ran Yoon, Kichul Cho, Seon-Jin Lee, José L. Crespo, Rishiram Ramanan, Hee-Sik Kim
Cells.2019; 8(9): 992. CrossRef
Evaluation of the potential of Chlorella sp. HS2, an algal isolate from a tidal rock pool, as an industrial algal crop under a wide range of abiotic conditions
Jin-Ho Yun, Dae-Hyun Cho, Jina Heo, Yong Jae Lee, Bongsoo Lee, Yong Keun Chang, Hee-Sik Kim
Journal of Applied Phycology.2019; 31(4): 2245. CrossRef
Maximizing biomass and lipid production in Ettlia sp. by ultraviolet stress in a continuous culture
Seong-Hyun Seo, Ankita Srivastava, Myung-Soo Han, Hyung-Gwan Lee, Hee-Mock Oh
Bioresource Technology.2019; 288: 121472. CrossRef
Identification of significant proxy variable for the physiological status affecting salt stress-induced lipid accumulation in Chlorella sorokiniana HS1
Seung Hwan Oh, Yong Keun Chang, Jay Hyung Lee
Biotechnology for Biofuels.2019;[Epub] CrossRef
Isolation and genome analysis of Winogradskyella algicola sp. nov., the dominant bacterial species associated with the green alga Dunaliella tertiolecta
Jaeho Song, Yeonjung Lim, Hye-Jin Jang, Yochan Joung, Ilnam Kang, Seong-Joo Hong, Choul-Gyun Lee, Jang-Cheon Cho
Journal of Microbiology.2019; 57(11): 982. CrossRef
Nitrogen modulation under chemostat cultivation mode induces biomass and lipid production by Chlorella vulgaris and reduces antenna pigment accumulation
Kichul Cho, Dae-Hyun Cho, Jina Heo, Urim Kim, Yong Jae Lee, Dong-Yun Choi, Hee-Sik Kim
Bioresource Technology.2019; 281: 118. CrossRef
Optimization of microalgal growth and cultivation parameters for increasing bioenergy potential: Case study using the oleaginous microalga Chlorella pyrenoidosa Chick (IPPAS C2)
Kateřina Sukačová, Diana Búzová, Petr Trávníček, Jan Červený, Monika Vítězová, Tomáš Vítěz
Algal Research.2019; 40: 101519. CrossRef
Application of biosurfactant from Bacillus subtilis C9 for controlling cladoceran grazers in algal cultivation systems
Jin-Ho Yun, Dae-Hyun Cho, Bongsoo Lee, Hee-Sik Kim, Yong Keun Chang
Scientific Reports.2018;[Epub] CrossRef

Research Support, Non-U.S. Gov'ts

NOTE] Quantification of Toxic Effects of the Herbicide Metolachlor on Marine Microalgae Ditylum brightwellii (Bacillariophyceae), Prorocentrum minimum (Dinophyceae), and Tetraselmis suecica (Chlorophyceae): Vinitha Ebenezer , Jang-Seu Ki; J. Microbiol. 2013;51(1):136-139. Published online March 2, 2013; DOI: https://doi.org/10.1007/s12275-013-2114-0

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Abstract: Toxic effects of the herbicide metolachlor (MC) were evaluated for three marine microalgae, Tetraselmis suecica (chlorophyte), Ditylum brightwellii (diatom), and Prorocentrum minimum (dinoflagellate). MC showed a significant reduction in cell counts and chlorophyll a levels. Median effective concentration (EC50) was calculated based on chlorophyll a levels after a 72-h MC exposure. EC50 values for T. suecica, D. brightwellii, and P. minimum were 21.3, 0.423, and 0.07 mg/L, respectively. These values showed that the dinoflagellate was most sensitive when exposed to the herbicide, at a concentration comparable to freshwater algae, suggesting its potential as an appropriate model organism for ecotoxicity assessments in marine environments.

Accumulation of Lipid Production in Chlorella minutissima by Triacylglycerol Biosynthesis-Related Genes Cloned from Saccharomyces cerevisiae and Yarrowia lipolytica: Hsin-Ju Hsieh , Chia-Hung Su , Liang-Jung Chien; J. Microbiol. 2012;50(3):526-534. Published online June 30, 2012; DOI: https://doi.org/10.1007/s12275-012-2041-5

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Abstract

Discovery of an alternative fuel is now an urgent matter because of the impending issue of oil depletion. Lipids synthesized in algal cells called triacylglycerols (TAGs) are thought to be of the most value as a potential biofuel source because they can use transesterification to manufacture biodiesel. Biodiesel is deemed as a good solution to overcoming the problem of oil depletion since it is capable of providing good performance similar to that of petroleum. Expression of several genomic sequences, including glycerol-3-phosphate dehydrogenase, glycerol-3-phosphate acyltransferase, lysophosphatidic acid acyltransferase, phosphatidic acid phosphatase, diacylglycerol acyltransferase, and phospholipid:diacylglycerol acyltransferase, can be useful for manipulating metabolic pathways for biofuel production. In this study, we found this approach indeed increased the storage lipid content of C. minutissima UTEX 2219 up to 2-fold over that of wild type. Thus, we conclude this approach can be used with the biodiesel production platform of C. minutissima UTEX 2219 for high lipid production that will, in turn, enhance productivity.

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Sustainable Energy Technologies and Assessments.2025; 74: 104190. CrossRef
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Chuchi Chen, Valerie C. A. Ward
SynBio.2024; 2(2): 223. CrossRef
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Johanna Becker, Ulf W Liebal, An NT Phan, Lena Ullmann, Lars M Blank
Current Opinion in Biotechnology.2023; 79: 102849. CrossRef
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Prachi Nawkarkar, Vikas U. Kapase, Sarika Chaudhary, Sachin Kajla, Shashi Kumar
GCB Bioenergy.2023; 15(10): 1240. CrossRef
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Enver Ersoy Andeden, Sahlan Ozturk, Belma Aslim
Biocatalysis and Agricultural Biotechnology.2023; 48: 102628. CrossRef
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Phytochemistry Reviews.2023; 22(4): 993. CrossRef
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Biocatalysis and Agricultural Biotechnology.2023; 53: 102877. CrossRef
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Rupal Gupta, Srijoni Banerjee, Soumya Pandit, Piyush Kumar Gupta, Abhilasha Singh Mathriya, Sanjay Kumar, Dibyajit Lahiri, Moupriya Nag, Rina Rani Ray, Sanket Joshi
Biomass Conversion and Biorefinery.2023; 13(17): 15357. CrossRef
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Frontiers in Microbiology.2023;[Epub] CrossRef
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Anjani Devi Chintagunta, N. S. Sampath Kumar, P. Naveen Kumar, P. Senthil Kumar, S.P. Jeevan Kumar
SSRN Electronic Journal.2022;[Epub] CrossRef
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Zhi Zhu, Jing Sun, Yun Fa, Xufeng Liu, Peter Lindblad
Frontiers in Microbiology.2022;[Epub] CrossRef
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Lakhan Kumar, Lalit Mohan, Raksha Anand, Vandana Joshi, Mohita Chugh, Navneeta Bharadvaja
Frontiers in Chemical Engineering.2022;[Epub] CrossRef
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Jyoti Rawat, Piyush Kumar Gupta, Soumya Pandit, Kanu Priya, Daksh Agarwal, Manu Pant, Vijay Kumar Thakur, Veena Pande
Energies.2022; 15(4): 1550. CrossRef
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Nam Kyu Kang, Kwangryul Baek, Hyun Gi Koh, Christine Anne Atkinson, Donald R. Ort, Yong-Su Jin
Bioresource Technology.2022; 345: 126529. CrossRef
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Adarsha Gupta, Colin J. Barrow, Munish Puri
Trends in Biotechnology.2022; 40(4): 448. CrossRef
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Swathi Somaiyan Babu, Rashmi Gondi, Godvin Sharmila Vincent, Godwin Christopher JohnSamuel, Rajesh Banu Jeyakumar
Sustainability.2022; 14(22): 15070. CrossRef
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Anna Santin, Monia Teresa Russo, Maria Immacolata Ferrante, Sergio Balzano, Ida Orefice, Angela Sardo
Molecules.2021; 26(24): 7697. CrossRef
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Wei Xing, Ruihao Zhang, Qun Shao, Chunxiao Meng, Xiaodong Wang, Zuoxi Wei, Fengjie Sun, Chang Wang, Kai Cao, Bingkui Zhu, Zhengquan Gao
Agronomy.2021; 11(5): 961. CrossRef
Insights into the genetic and metabolic engineering approaches to enhance the competence of microalgae as biofuel resource: A review
Amandeep Brar, Manish Kumar, Twinkle Soni, V Vivekanand, Nidhi Pareek
Bioresource Technology.2021; 339: 125597. CrossRef
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Han Sun, Tao Wu, Stephenie Hiu Yuet Chen, Yuanyuan Ren, Shufang Yang, Junchao Huang, Haijin Mou, Feng Chen
Renewable and Sustainable Energy Reviews.2021; 152: 111609. CrossRef
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Critical Reviews in Food Science and Nutrition.2020; 60(19): 3195. CrossRef
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Zahra Shokravi, Hoofar Shokravi, Ong Hwai Chyuan, Woei Jye Lau, Seyed Saeid Rahimian Koloor, Michal Petrů, Ahmad Fauzi Ismail
Sustainability.2020; 12(21): 9083. CrossRef
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Gulshan Kumar, Ajam Shekh, Sunaina Jakhu, Yogesh Sharma, Ritu Kapoor, Tilak Raj Sharma
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Susrita Sahoo, Soumya R. Mahapatra, Nayanika Das, Bikram K. Parida, Satyajit Rath, Namrata Misra, Mrutyunjay Suar
Algal Research.2020; 47: 101887. CrossRef
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Ayesha Shahid, Abd ur Rehman, Muhammad Usman, Muhammad Umer Farooq Ashraf, Muhammad Rizwan Javed, Aqib Zafar Khan, Saba Shahid Gill, Muhammad Aamer Mehmood
Biotechnology and Applied Biochemistry.2020; 67(1): 41. CrossRef
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Biofuels.2019; 10(1): 45. CrossRef
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Fatty Acid Biosynthesis in Eukaryotic Photosynthetic Microalgae: Identification of a Microsomal Delta 12 Desaturase in Chlamydomonas reinhardtii: Xiaoyuan Chi , Xiaowen Zhang , Xiangyu Guan , Ling Ding , Youxun Li , Mingqing Wang , Hanzhi Lin , Song Qin; J. Microbiol. 2008;46(2):189-201. Published online June 11, 2008; DOI: https://doi.org/10.1007/s12275-007-0223-3

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Abstract

Polyunsaturated fatty acids (PUFAs) are important components of infant and adult nutrition because they serve as structural elements of cell membranes. Fatty acid desaturases are responsible for the insertion of double bonds into pre-formed fatty acid chains in reactions that require oxygen and reducing equivalents. In this study, the genome-wide characterization of the fatty acid desaturases from seven eukaryotic photosynthetic microalgae was undertaken according to the conserved histidine-rich motifs and phylogenetic profiles. Analysis of these genomes provided insight into the origin and evolution of the pathway of fatty acid biosynthesis in eukaryotic plants. In addition, the candidate enzyme from Chlamydomonas reinhardtii with the highest similarity to the microsomal Δ12 desaturase of Chlorella vulgaris was isolated, and its function was verified by heterologous expression in yeast (Saccharomyces cerevisiae).

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Genome-wide analysis of fatty acid desaturase genes in rice (Oryza sativa L.)
Zhiguo E, Chen Chen, Jinyu Yang, Hanhua Tong, Tingting Li, Lei Wang, Hongqi Chen
Scientific Reports.2019;[Epub] CrossRef
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Chetan Paliwal, Madhusree Mitra, Khushbu Bhayani, S.V. Vamsi Bharadwaj, Tonmoy Ghosh, Sonam Dubey, Sandhya Mishra
Bioresource Technology.2017; 244: 1216. CrossRef
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De novo transcriptome analysis of carotenoid and polyunsaturated fatty acid metabolism in Rhodomonas sp.
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Journal of Applied Phycology.2016; 28(3): 1649. CrossRef
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Joris Beld, Raffaela Abbriano, Kara Finzel, Mark Hildebrand, Michael D. Burkart
Molecular BioSystems.2016; 12(4): 1299. CrossRef
Cisgenesis and intragenesis in microalgae: promising advancements towards sustainable metabolites production
Janeth I. Galarza, Ninoska Delgado, Vitalia Henríquez
Applied Microbiology and Biotechnology.2016; 100(24): 10225. CrossRef
Rapid Lipid Induction in Chlorella sp. by UV-C Radiation
Kalpesh K. Sharma, Yan Li, Peer M. Schenk
BioEnergy Research.2015; 8(4): 1824. CrossRef
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Yuval Kaye, Omer Grundman, Stefan Leu, Aliza Zarka, Boris Zorin, Shoshana Didi-Cohen, Inna Khozin-Goldberg, Sammy Boussiba
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Yonghua Li‐Beisson, Fred Beisson, Wayne Riekhof
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Gabriel O. James, Charles H. Hocart, Warwick Hillier, Hancai Chen, Farzaneh Kordbacheh, G. Dean Price, Michael A. Djordjevic
Bioresource Technology.2011; 102(3): 3343. CrossRef
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Plant Cell Reports.2011; 30(8): 1393. CrossRef
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Journal of Phycology.2011; 47(4): 763. CrossRef
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Lipids.2010; 45(2): 179. CrossRef
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Biochimie.2009; 91(6): 679. CrossRef

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