Journal of Microbiology

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Calculibacillus koreensis gen. nov., sp. nov., an anaerobic Fe(III)-reducing bacterium isolated from sediment of mine tailings: Ui-Gi Min , So-Jeong Kim , Heeji Hong , Song-Gun Kim , Joo-Han Gwak , Man-Young Jung , Jong-Geol Kim , Jeong-Geol Na , Sung-Keun Rhee; J. Microbiol. 2016;54(6):413-419. Published online May 27, 2016; DOI: https://doi.org/10.1007/s12275-016-6086-8

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A strictly anaerobic bacterium, strain B5T, was isolated from sediment of an abandoned coal mine in Taebaek, Republic of Korea. Cells of strain B5T were non-spore-forming, straight, Gram-positive rods. The optimum pH and temperature for growth were pH 7.0 and 30°C, respectively, while the strain was able to grow within pH and temperature ranges of 5.5– 7.5 and 25–45°C, respectively. Growth of strain B5T was observed at NaCl concentrations of 0 to 6.0% (w/v) with an optimum at 3.0–4.0% (w/v). The polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol, an unknown phospholipid and three unknown polar lipids. Strain B5T grew anaerobically by reducing nitrate, nitrite, ferric-citrate, ferric-nitrilotriacetate, elemental sulfur, thiosulfate, and anthraquinone- 2-sulfonate in the presence of proteinaceous compounds, organic acids, and carbohydrates as electron donors. The isolate was not able to grow by fermentation. Strain B5T did not grow under aerobic or microaerobic conditions. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain B5T is most closely related to the genus Tepidibacillus (T. fermentans STGHT; 96.3%) and Vulcanibacillus (V. modesticaldus BRT; 94.6%). The genomic DNA G+C content (36.9 mol%) of strain B5T was higher than those of T. fermentans STGHT (34.8 mol%) and V. modesticaldus BRT (34.5 mol%). Based on its phenotypic, chemotaxonomic, and phylogenetic properties, we describe a new species of a novel genus Calculibacillus, represented by strain B5T (=KCTC 15397T =JCM 19989T), for which we propose the name Calculibacillus koreensis gen. nov., sp. nov.

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Bacillales: From Taxonomy to Biotechnological and Industrial Perspectives
Sharareh Harirchi, Taner Sar, Mohaddaseh Ramezani, Habibu Aliyu, Zahra Etemadifar, Seyed Ali Nojoumi, Fatemeh Yazdian, Mukesh Kumar Awasthi, Mohammad J. Taherzadeh
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List of new names and new combinations that have appeared in effective publications outside of the IJSEM and are submitted for valid publication
Aharon Oren, George M. Garrity
International Journal of Systematic and Evolutionary Microbiology .2019;[Epub] CrossRef
Complete genome sequence of Clostridium perfringens CBA7123 isolated from a faecal sample from Korea
Yeon Bee Kim, Joon Yong Kim, Hye Seon Song, Changsu Lee, Joseph Kwon, Jisu Kang, Jin-Kyu Rhee, Myeong Seon Jeong, Young-Do Nam, Seong Woon Roh
Gut Pathogens.2017;[Epub] CrossRef

Research Support, Non-U.S. Gov'ts

Bacterial Diversity and Composition of an Alkaline Uranium Mine Tailings-Water Interface: Nurul H. Khan , Viorica F. Bondici , Prabhakara G. Medihala , John R. Lawrence , Gideon M. Wolfaardt , Jeff Warner , Darren R. Korber; J. Microbiol. 2013;51(5):558-569. Published online September 14, 2013; DOI: https://doi.org/10.1007/s12275-013-3075-z

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Abstract

The microbial diversity and biogeochemical potential associated with a northern Saskatchewan uranium mine watertailings interface was examined using culture-dependent and -independent techniques. Morphologically-distinct colonies from uranium mine water-tailings and a reference lake (MC) obtained using selective and non-selective media were selected for 16S rRNA gene sequencing and identification, revealing that culturable organisms from the uranium tailings interface were dominated by Firmicutes and Betaproteobacteria; whereas, MC organisms mainly consisted of Bacteroidetes and Gammaproteobacteria. Ion Torrent (IT) 16S rRNA metagenomic analysis carried out on extracted DNA from tailings and MC interfaces demonstrated the dominance of Firmicutes in both of the systems. Overall, the tailings-water interface environment harbored a distinct bacterial community relative to the MC, reflective of the ambient conditions (i.e., total dissolved solids, pH, salinity, conductivity, heavy metals) dominating the uranium tailings system. Significant correlations among the physicochemical data and the major bacterial groups present in the tailings and MC were also observed. Presence of sulfate reducing bacteria demonstrated by culture-dependent analyses and the dominance of Desulfosporosinus spp. indicated by Ion Torrent analyses within the tailings-water interface suggests the existence of anaerobic microenvironments along with the potential for reductive metabolic processes.

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Environmental Microbiome.2023;[Epub] CrossRef
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Jianping Lu, Rongyue Geng, Han Zhang, Zhen Yu, Tao Chen, Baogang Zhang
Journal of Hazardous Materials.2023; 452: 131222. CrossRef
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Uday Kumar Banala, Nilamadhab Prasad Indradyumna Das, Subba Rao Toleti
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Alkaliphilic bacteria retrieved from uranium mining effluent: Characterization, U sequestration and remediation potential
Uday Kumar Banala, Nilamadhab Prasad Indradyumna Das, Ranjib Kumar Padhi, Subba Rao Toleti
Environmental Technology & Innovation.2021; 24: 101893. CrossRef
Geochemistry of uranium mill tailings in the Athabasca Basin, Saskatchewan, Canada: A review
Jared Robertson, M. Jim Hendry, T. Kotzer, Kebbi A. Hughes
Critical Reviews in Environmental Science and Technology.2019; 49(14): 1237. CrossRef
In vitro biomedicinal properties of Pyrrolidine-2,4-Dione derived from a novel actinobacterium Streptomyces rochei, a green approach
Mookkan Bodhaguru, Prakash Santhiyagu, Mariselvam Lakshmanan, Ramasubburayan Ramasamy, Ahila Natarajan Kumari, Kannapiran Ethiraj, Palavesam Arunachalam, Immanuel Grasian
Biocatalysis and Agricultural Biotechnology.2019; 20: 101244. CrossRef
Nutrients Availability Shapes Fungal Community Composition and Diversity in the Rare Earth Mine Tailings of Southern Jiangxi, China
Genhe He, Xiaodong Wang, Xu Liu, Xiaoyu Xiao, Shoucheng Huang, Jichun Wu
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Biogeochemical Importance of the Bacterial Community in Uranium Waste Deposited at Key Lake, Northern Saskatchewan
Viorica F. Bondici, George D. W. Swerhone, James J. Dynes, John R. Lawrence, Gideon M. Wolfaardt, Jeff Warner, Darren R. Korber
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Biogeochemical activity of microbial biofilms in the water column overlying uranium mine tailings
V.F. Bondici, N.H. Khan, G.D.W. Swerhone, J.J. Dynes, J.R. Lawrence, E. Yergeau, G.M. Wolfaardt, J. Warner, D.R. Korber
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Arsenite Oxidation by a Facultative Chemolithotrophic Bacterium SDB1 Isolated from Mine Tailing: Rovimar T. Lugtu , Sung-Chan Choi , Young-Sook Oh; J. Microbiol. 2009;47(6):686-692. Published online February 4, 2010; DOI: https://doi.org/10.1007/s12275-009-0279-3

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Abstract: An arsenite (As[III])-oxidizing bacterium, SDB1, was isolated from mine tailing collected from the Sangdong mine area in Korea and showed chemolithotrophic growth on As[III] and CO2 as the respective electron and carbon sources. SDB1 is Gram-negative, rod-shaped, and belongs to the Sinorhizobium-Ensifer branch of α-Proteobacteria. Growth and As[III] oxidation was enhanced significantly by the presence of yeast extract (0.005%) in minimal salt medium containing 5 mM As[III]; decreasing the doubling time from 9.8 to 2.1 h and increasing the As[III] oxidation rate from 0.014 to 0.349 pmol As[III] oxidized cell-1 h-1. As[III] oxidation nearly stopped at pH around 4 and should be performed at pH 7~8 to be most effective. SDB1 was immobilized in calcium-alginate beads and the oxidation capacity was investigated. Specific As[III] oxidation rates obtained with SDB1 (10.1~33.7 mM As[III] oxidized g-1 dry cell h-1) were 10~16-times higher than those reported previously with a heterotrophic bacterial strain (Simeonova et al., 2005). The stability and reusability of immobilized SDB1 strongly suggested that the immobilized SDB1 cell system can make the As[III] oxidation process technically and economically feasible in practical applications.

Bacterial Diversity at Different Depths in Lead-Zinc Mine Tailings as Revealed by 16S rRNA Gene Libraries: Han-Bo Zhang , Wen Shi , Ming-Xia Yang , Tao Sha , Zhi-Wei Zhao; J. Microbiol. 2007;45(6):479-484.; DOI: https://doi.org/2648 [pii]

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Abstract: Bacterial communities at 10 cm, 100 cm, and 200 cm depths in a 100-year-old lead-zinc tailing heap were evaluated by constructing 16S rRNA gene libraries. In total, 98 operational taxonomic units (OTUs) were identified from 193 clones at a 3% sequence difference level. The OTU number and species richness decreased with the depth. Species composition was significantly different between the three libraries. Fifty-seven percent of the examined clones were Acidobacteria and 27% belonged to Proteobacteria. Other sequences included Chloroflexi, Firmicutes, Chlamydiae, Actinobacteria, Gemmatimonadetes, Nitrospira, and three unclassified OTUs. Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, Firmicutes, and Actinobacteria were mainly distributed in the rhizosphere of naturally colonizing plants; however, Deltaproteobacteria, Acidobacteria, and Chloroflexi tended to inhabit the deeper tailings (below the 100 cm-depth).

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