Journal of Microbiology

Research Support, Non-U.S. Gov't

Differential Expression of citA Gene Encoding the Mitochondrial Citrate Synthase of Aspergillus nidulans in Response to Developmental Status and Carbon Sources: In Sook Min , Ji Young Bang , Soon Won Seo , Cheong Ho Lee , Pil Jae Maeng; J. Microbiol. 2010;48(2):188-198. Published online May 1, 2010; DOI: https://doi.org/10.1007/s12275-010-0096-8

53 View
0 Download
8 Crossref

As an extension of our previous studies on the mitochondrial citrate synthase of Aspergillus nidulans and cloning of its coding gene (citA), we analyzed differential expression of citA in response to the progress of development and change of carbon source. The cDNA consisted of 1,700 nucleotides and was predicted to encode a 474-amino acid protein. By comparing the cDNA sequence with the corresponding genomic sequence, we confirmed that citA gene contains 7 introns and that its transcription starts at position -26 (26-nucleotide upstream from the initiation codon). Four putative CreA binding motifs and three putative stress-response elements (STREs) were found within the 1.45-kb citA promoter region. The mode of citA expression was examined by both Northern blot and confocal microscopy using green fluorescent protein (sGFP) as a vital reporter. During vegetative growth and asexual development, the expression of citA was ubiqiutous throughout the whole fungal body including mycelia and conidiophores. During sexual development, the expression of citA was quite strong in cleistothecial shells, but significantly weak in the content of cleistothecia including ascospores. Acetate showed a strong inductive effect on citA expression, which is subjected to carbon catabolite repression (CCR) caused by glucose. The recombinant fusion protein CitA40::sGFP (sGFP containing the 40-amino acid N-terminal segment of CitA) was localized into mitochondria, which supports that a mitochondrial targeting signal is included within the 40-amino acid N-terminal segment of CitA.

Citations

Citations to this article as recorded by

Efficient carbon flux allocation towards D-pantothenic acid production via growth-decoupled strategy in Escherichia coli
Yihong Wang, Junping Zhou, Zheng Zhang, Lianggang Huang, Bo Zhang, Zhiqiang Liu, Yuguo Zheng
Bioresource Technology.2024; 411: 131325. CrossRef
Shining a light on the impact of antifungals on Aspergillus fumigatus subcellular dynamics through fluorescence imaging
I. S. R. Storer, L. E. Sastré-Velásquez, T. Easter, B. Mertens, A. Dallemulle, M. Bottery, R. Tank, M. Offterdinger, M. J. Bromley, N. van Rhijn, F. Gsaller, Andreas H. Groll
Antimicrobial Agents and Chemotherapy.2024;[Epub] CrossRef
Multiplex Genetic Engineering Exploiting Pyrimidine Salvage Pathway-Based Endogenous Counterselectable Markers
Lukas Birštonas, Alex Dallemulle, Manuel S. López-Berges, Ilse D. Jacobsen, Martin Offterdinger, Beate Abt, Maria Straßburger, Ingo Bauer, Oliver Schmidt, Bettina Sarg, Herbert Lindner, Hubertus Haas, Fabio Gsaller, Gustavo H. Goldman
mBio.2020;[Epub] CrossRef
A novel citrate synthase isoform contributes infection and stress resistance of the stripe rust fungus
Dan Li, Lijing Pang, Pu Yuan, Peijing Zheng, Baoyu Huai, Mohan Yao, Zhensheng Kang, Jie Liu
Environmental Microbiology.2018; 20(11): 4037. CrossRef
In silico characterization of the citrate synthase family in Mycobacterium tuberculosis / Mycobacterium tuberculosis’te sitrat sentaz ailesinin in silico karakterizasyonu
Sezer Okay
Turkish Journal of Biochemistry.2016; 41(2): 118. CrossRef
Three genes encoding citrate synthases in Saccharopolyspora erythraea are regulated by the global nutrient‐sensing regulators GlnR, DasR, and CRP
Cheng‐Heng Liao, Li‐li Yao, Bang‐Ce Ye
Molecular Microbiology.2014; 94(5): 1065. CrossRef
Gene Identification and Functional Analysis of Methylcitrate Synthase in Citric Acid-ProducingAspergillus nigerWU-2223L
Keiichi KOBAYASHI, Takasumi HATTORI, Yuki HONDA, Kohtaro KIRIMURA
Bioscience, Biotechnology, and Biochemistry.2013; 77(7): 1492. CrossRef
Utility of Aspergillus niger citrate synthase promoter for heterologous expression
Kashyap Dave, Narayan S. Punekar
Journal of Biotechnology.2011; 155(2): 173. CrossRef

First
Prev
Page of 1
Next
Last

Search