Journal of Microbiology

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Kinetic characterization of laccase from Bacillus atrophaeus, and its potential in juice clarification in free and immobilized forms: Lokesh Kumar Narnoliya , Neera Agarwal , Satya N. Patel , Sudhir P. Singh; J. Microbiol. 2019;57(10):900-909. Published online August 28, 2019; DOI: https://doi.org/10.1007/s12275-019-9170-z

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In the present study, a laccase gene (BaLc) from a lignin degrading bacterium, Bacillus atrophaeus, has been cloned and expressed in Escherichia coli. The optimal catalytic activity of the protein was achieved at 5.5 pH and 35°C temperature, measured by oxidation of ABTS. The Km and Vmax values were determined as 1.42 mM and 4.16 μmole/min, respectively. To achieve the enzyme recovery, the biocatalyst (BaLc) was covalently attached onto the functionalized iron magnetic-nanoparticles. The nanoparticles were characterized by zeta-potential and FTIR analyses. The immobilized BaLc enzyme was physico-kinetically characterized, exhibiting retention of 60% of the residual activity after ten reaction cycles of ABTS oxidation. The immobilized biocatalyst system was tested for its biotechnological exploitability in plant juice processing, achieving 41–58% of phenol reduction, 41–58% decolorization, 50–59% turbidity reduction in the extracts of banana pseudo-stem and sweet sorghum stalk, and apple fruit juice. This is the first study to demonstrate the use of nanoparticle- laccase conjugate in juice clarification. The findings suggest that B. atrophaus laccase is a potential catalytic tool for plant juice bioprocessing activities.

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Immobilized enzymes: exploring its potential in food industry applications
K. A. Jothyswarupha, Swethaa Venkataraman, Devi Sri Rajendran, S. S. Sakthi Shri, Shivani Sivaprakasam, Tholeti Yamini, P. Karthik, Vaidyanathan Vinoth Kumar
Food Science and Biotechnology.2024;[Epub] CrossRef
Harnessing the power of bacterial laccases for xenobiotic degradation in water: A 10-year overview
Mujeeb ur Rahman, Muhammad Wajid Ullah, Junaid Ali Shah, Sivasamy Sethupathy, Hazart Bilal, Sidikov Akmal Abdikakharovich, Afaq Ullah Khan, Khalid Ali Khan, Noureddine Elboughdiri, Daochen Zhu
Science of The Total Environment.2024; 918: 170498. CrossRef
Laccase-mediated degradation of emerging contaminants: unveiling a sustainable solution
Pooja Thathola, Elda M. Melchor-Martínez, Priyanka Adhikari, Saúl Antonio Hernández Martínez, Anita Pandey, Roberto Parra-Saldívar
Environmental Science: Advances.2024; 3(11): 1500. CrossRef
Biochemical Characteristics of Laccases and Their Practical Application in the Removal of Xenobiotics from Water
Agnieszka Gałązka, Urszula Jankiewicz, Andrzej Szczepkowski
Applied Sciences.2023; 13(7): 4394. CrossRef
Application of Immobilized Enzymes in Juice Clarification
Feng Wang, Hui Xu, Miaomiao Wang, Xiaolei Yu, Yi Cui, Ling Xu, Anzhou Ma, Zhongyang Ding, Shuhao Huo, Bin Zou, Jingya Qian
Foods.2023; 12(23): 4258. CrossRef
Bacterial Laccases as Biocatalysts for the Remediation of Environmental Toxic Pollutants: A Green and Eco-Friendly Approach—A Review
Neha Agarwal, Vijendra Singh Solanki, Amel Gacem, Mohd Abul Hasan, Brijesh Pare, Amrita Srivastava, Anupama Singh, Virendra Kumar Yadav, Krishna Kumar Yadav, Chaigoo Lee, Wonjae Lee, Sumate Chaiprapat, Byong-Hun Jeon
Water.2022; 14(24): 4068. CrossRef
Enzymatic treatment, unfermented and fermented fruit-based products: current state of knowledge
Joanne Yi Hui Toy, Yuyun Lu, Dejian Huang, Keisuke Matsumura, Shao-Quan Liu
Critical Reviews in Food Science and Nutrition.2022; 62(7): 1890. CrossRef
Current research progress on laccase-like nanomaterials
Lulu Lei, Xiaoyu Yang, Yudong Song, Hui Huang, Yongxin Li
New Journal of Chemistry.2022; 46(8): 3541. CrossRef
Poly(vinyl Alcohol)-Alginate Immobilized Trametes versicolor IBL-04 Laccase as Eco-friendly Biocatalyst for Dyes Degradation
Sadia Noreen, Muhammad Asgher, Sarmad Ahmad Qamar, Muhammad Bilal, Hafiz M. N. Iqbal
Catalysis Letters.2022; 152(6): 1869. CrossRef
Recent developments in enzyme immobilization technology for high-throughput processing in food industries
Asghar Taheri-Kafrani, Sara Kharazmi, Mahmoud Nasrollahzadeh, Asieh Soozanipour, Fatemeh Ejeian, Parisa Etedali, Hajar-Alsadat Mansouri-Tehrani, Amir Razmjou, Samaneh Mahmoudi-Gom Yek, Rajender S. Varma
Critical Reviews in Food Science and Nutrition.2021; 61(19): 3160. CrossRef
Laccases in food processing: Current status, bottlenecks and perspectives
Emanueli Backes, Camila Gabriel Kato, Rúbia Carvalho Gomes Corrêa, Regina de Fátima Peralta Muniz Moreira, Rosely Aparecida Peralta, Lillian Barros, Isabel C.F.R. Ferreira, Gisella Maria Zanin, Adelar Bracht, Rosane Marina Peralta
Trends in Food Science & Technology.2021; 115: 445. CrossRef
A novel Bacillus ligniniphilus catechol 2,3-dioxygenase shows unique substrate preference and metal requirement
Peter Adewale, Alice Lang, Fang Huang, Daochen Zhu, Jianzhong Sun, Michael Ngadi, Trent Chunzhong Yang
Scientific Reports.2021;[Epub] CrossRef
Laccases as green and versatile biocatalysts: from lab to enzyme market—an overview
Tatiane Brugnari, Dayane Moreira Braga, Camila Souza Almeida dos Santos, Bruno Henrique Czelusniak Torres, Tatiani Andressa Modkovski, Charles Windson Isidoro Haminiuk, Giselle Maria Maciel
Bioresources and Bioprocessing.2021;[Epub] CrossRef
Extracting flavonoid from Ginkgo biloba using lignocellulolytic bacteria Paenarthrobacter sp. and optimized via response surface methodology
Sihai Han, Chonlong Chio, Tianxiao Ma, Aristide Laurel Mokale Kognou, Sarita Shrestha, Feifei Chen, Wensheng Qin
Biofuels, Bioproducts and Biorefining.2021; 15(3): 867. CrossRef
Magnetic nanobiocatalyst for extraction of bioactive ingredients: A novel approach
Shamraja S. Nadar, Pravin D. Patil, Nanda M. Rohra
Trends in Food Science & Technology.2020; 103: 225. CrossRef

Production of an Endoinulinase from Aspergillus niger AUMC 9375, by Solid State Fermentation of Agricultural Wastes, with Purification and Characterization of the Free and Immobilized Enzyme: Manal M. Housseiny; J. Microbiol. 2014;52(5):389-398. Published online May 9, 2014; DOI: https://doi.org/10.1007/s12275-014-3561-y

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Abstract

Two different substrates, sunflower (Helianthus annuus L.) tubers and lettuce (Lactuca sativa) roots, were tested. Using a mixture of both wastes resulted in higher production of endoinulinase than either waste alone. Also, ten fungal spe-cies grown on these substrates as inexpensive, carbon sour-ces were screened for the best production of endoinulinase activities. Of these, Aspergillus niger AUMC 9375 was the most productive, when grown on the mixture using a 6:1 w/w ratio of sun flower: lettuce, and yielded the highest levels of inulinase at 50% moisture, 30°C, pH 5.0, with seven days of incubation, and with yeast extract as the best nitrogen source. Inulinase was purified to homogeneity by ion-exchange chro-matography and gel-filtration giving a 51.11 fold purification. The mixture of sunflower tubers and lettuce roots has poten-tial to be an effective and economical substrate for inulinase production. Inulinase was successfully immobilized with an immobilization yield of 71.28%. After incubation for 2 h at 60°C, the free enzyme activity decreased markedly to 10%, whereas that of the immobilized form decreased only to 87%. A reusability test demonstrated the durability of the immo-bilized inulinase for 10 cycles and in addition, that it could be stored for 32 days at 4°C. These results indicate that this inulinase, in the immobilized form, is a potential candidate for large-scale production of high purity fructose syrups.

Citations

Citations to this article as recorded by

Production of β-mannanase, inulinase, and oligosaccharides from coffee wastes and extracts
Selin Basmak, Irfan Turhan
International Journal of Biological Macromolecules.2024; 261: 129798. CrossRef
Sustainable inulinase enzyme production from novel strain Fusarium parceramosum with mixed biomass substrates of rice husk and banana shoot through solid-state fermentation
Shreya Hegde, Ramananda Bhat M, Subbalaxmi Selvaraj
Biomass Conversion and Biorefinery.2024;[Epub] CrossRef
Bio-utilization of agricultural residue banana plant shoot through solid state fermentation for production of inulinase using newly isolated Nothophoma anigozanthi JAM
Nisarga Tippanavar, Divya Bhat, Orline Rebello, Girisa Prabhu, Subbalaxmi Selvaraj, Ramananda M. Bhat
Biomass Conversion and Biorefinery.2024; 14(13): 14755. CrossRef
Aspergillus welwitschiae inulinase enzyme cocktails obtained on agro-material inducers for the purpose of fructooligosaccharides production
Sanja Stojanović, Marina Ristović, Jelena Stepanović, Aleksandra Margetić, Bojan Duduk, Zoran Vujčić, Biljana Dojnov
Food Research International.2022; 160: 111755. CrossRef
Solid-state fermentation enhances inulinase and invertase production by Aspergillus brasiliensis
C. Guerrero-Urrutia, T. Volke-Sepulveda, F. Figueroa-Martinez, E. Favela-Torres
Process Biochemistry.2021; 108: 169. CrossRef
Statistical optimization of solid-state fermentation for the production of fungal inulinase from apple pomace
Ram Sarup Singh, Kanika Chauhan, Karminder Kaur, Ashok Pandey
Bioresource Technology Reports.2020; 9: 100364. CrossRef
Optimization of inulinase production by a newly isolated strain Aspergillus flavus var. flavus by solid state fermentation of Saccharum arundinaceum
Deblina Das, Raja Selvaraj, M. Ramananda Bhat
Biocatalysis and Agricultural Biotechnology.2019; 22: 101363. CrossRef
Review of inulinase production using solid-state fermentation
Deblina Das, Ramananda Bhat M, Raja Selvaraj
Annals of Microbiology.2019; 69(3): 201. CrossRef
Immobilized inulinase: a new horizon of paramount importance driving the production of sweetener and prebiotics
Gerard Neeraj, Shobana Ravi, Ravindran Somdutt, ShriAishvarya Kaliyur Ravi, Vaidyanathan Vinoth Kumar
Critical Reviews in Biotechnology.2018; 38(3): 409. CrossRef
The cell wall anchored β-fructosidases of Lactobacillus paracasei : Overproduction, purification, and gene expression control
Petya Velikova, Kaloyan Petrov, Penka Petrova
Process Biochemistry.2017; 52: 53. CrossRef
Continuous generation of fructose from Taraxacum officinale tap root extract and inulin by immobilized inulinase in a packed-bed reactor
Hemant Kumar Rawat, Hemant Soni, Naveen Kango, C. Ganesh Kumar
Biocatalysis and Agricultural Biotechnology.2017; 9: 134. CrossRef
Biotechnological potential of microbial inulinases: Recent perspective
Hemant Kumar Rawat, Hemant Soni, Helen Treichel, Naveen Kango
Critical Reviews in Food Science and Nutrition.2017; 57(18): 3818. CrossRef
Development of heterogeneous preparation with inulinase for tubular reactor systems
M.G. Holyavka, M.P. Evstigneev, V.G. Artyukhov, V.V. Savin
Journal of Molecular Catalysis B: Enzymatic.2016; 129: 1. CrossRef
Recent insights in enzymatic synthesis of fructooligosaccharides from inulin
Ram Sarup Singh, Rupinder Pal Singh, John F. Kennedy
International Journal of Biological Macromolecules.2016; 85: 565. CrossRef
Production of inulinase, fructosyltransferase and sucrase from fungi on low-value inulin-rich substrates and their use in generation of fructose and fructo-oligosaccharides
Hemant Kumar Rawat, Mohd Anis Ganaie, Naveen Kango
Antonie van Leeuwenhoek.2015; 107(3): 799. CrossRef

Comparative Study of Olive Oil Mill Wastewater Treatment Using Free and Immobilized Coriolopsis polyzona and Pycnoporus coccineus: Mohamed Neifar , Atef Jaouani , María Jesús Martínez , Michel J. Penninckx; J. Microbiol. 2012;50(5):746-753. Published online November 4, 2012; DOI: https://doi.org/10.1007/s12275-012-2079-4

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Abstract: The efficiency of the two white-rot fungi Pycnoporus coccineus and Coriolopsis polyzona in the Olive Oil Mill Wastewater (OOMW) treatment was investigated. Both fungi were active in the decolourisation and COD removal of OOMW at 50 g/L COD, but only the first fungus remains effective on the crude effluent (COD=100 g/L). Moreover P. coccineus was less affected by oxygen supplementation and exhibited a high tolerance to agitation in comparison to C. polyzona. However, it required a nitrogen supplementation to obtain faster and higher COD removal. To overcome the negative effect of agitation on fungi growth and efficiency, immobilisation of C. polyzona and P. coccineus in polyurethane foam was applied. The immobilized system showed better COD decreases during three consecutive batches without remarkable loss of performances. The results obtained in this study suggested that immobilized C. polyzona and especially immobilized P. coccineus might be applicable to a large scale for the removal colour and COD of OOMW.

NOTE] Comparison of the Genetic Structures Surrounding qnrA1 in Korean Enterobacter cloacae and Chinese Escherichia coli Strains Isolated in the Early 2000s: Evidence for qnrA Mobilization via Inc HI2 Type Plasmid: Sang Hoon Han , Young Ah Kim , Minggui Wang , Yangsoon Lee , Hae-Sun Chung , Jong Hwa Yum , Dongeun Yong , Kyungwon Lee , June Myung Kim; J. Microbiol. 2012;50(1):166-169. Published online February 27, 2012; DOI: https://doi.org/10.1007/s12275-012-1350-z

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Abstract: The flanking genetic structure of qnrA1 in Korean Enterobacter cloacae was identical to that of the Chinese Escherichia coli strain, the first qnrA1-carrying strain reported in Asia. Analysis of restriction enzyme sites and Southern blot hybridization results showed that qnrA1 was transferred between E. cloacae and E. coli via Inc HI2 type plasmid.

Mobilization Functions of the Bacteriocinogenic Plasmid pRJ6 of Staphylococcus aureus: Marcus Livio Varella Coelho , Hilana Ceotto , Danielle Jannuzzi Madureira , Ingolf F. Nes , Maria do Carmo de Freire Bastos; J. Microbiol. 2009;47(3):327-336. Published online June 26, 2009; DOI: https://doi.org/10.1007/s12275-009-0044-7

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Abstract: Plasmid pRJ6 is the first known bacteriocinogenic mobilizable (Mob) plasmid of Staphylococcus aureus. Its Mob region is composed of four mob genes (mobCDAB) arranged as an operon, a genetic organization uncommon among S. aureus Mob plasmids. oriTpRJ6 was detected in a region of 431 bp, positioned immediately upstream of mobC. This region, when cloned into pCN37, was able to confer mobilization to the recombinant plasmid only in the presence of pRJ6. The entire Mob region, including oriTpRJ6, is much more similar to Mob regions from several coagulase-negative staphylococci plasmids, although some remarkable similarities with S. aureus Mob plasmids can also be noted. These similarities include the presence within oriTpRJ6 of the three mcb (MobC binding sites), firstly described in pC221 and pC223, an identical nick site also found in these same plasmids, and a nearly identical srapC223 site (sequence recognized by MobA). pRJ6 was successfully transferred to S. epidermidis by conjugation in the presence of the conjugative plasmid pGO1. Altogether these findings suggest that pRJ6 might have been originally a coagulase-negative staphylococci plasmid that had been transferred successfully to S. aureus.

Laccase Production Using Pleurotus ostreatus 1804 Immobilized on PUF Cubes in Batch and Packed Bed Reactors: Influence of Culture Conditions: K. Krishna Prasad , S. Venkata Mohan , Y. Vijaya Bhaskar , S. V. Ramanaiah , V. Lalit Babu , B. R. Pati , P. N. Sarma; J. Microbiol. 2005;43(3):301-307.; DOI: https://doi.org/2209 [pii]

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Abstract: The feasibility of laccase production by immobilization of Pleurotus ostreatus 1804 on polyurethane foam (PUF) cubes with respect to media composition was studied in both batch and reactor systems. Enhanced laccase yield was evidenced due to immobilization. A relatively high maximum laccase activity of 312.6 U was observed with immobilized mycelia in shake flasks compared to the maximum laccase activity of free mycelia (272.2 U). It is evident from this study that the culture conditions studied, i.e. biomass level, pH, substrate concentration, yeast extract concentration, Cu^2^+ concentration, and alcohol nature, showed significant influence on the laccase yield. Gel electrophoretic analysis showed the molecular weight of the laccase produced by immobilized P. ostreatus to be 66 kDa. The laccase yield was significantly higher and more rapid in the packed bed reactor than in the shake flask experiments. A maximum laccase yield of 392.9 U was observed within 144 h of the fermentation period with complete glucose depletion.

Microbial Metal Transformations: Geoffrey M. Gadd; J. Microbiol. 2001;39(2):83-88.

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Abstract: There is considerable interest in how microbiological processes can affect the behaviour of metal contaminants in natural and engineered environments and their potential for bioremediation. The extent to which microorganisms can affect metal contaminants is dependent on the identity and chemical form of the metal and the physical and chemical nature of the contaminated site or substance. In general terms, microbial processes which solubilize metals increase their bioavailability and potential toxicity, whereas those that immobilize them reduce bioavailability. The balance between mobilization and immobilization varies depending on the metal, the organisms, their environment and physico-chemical conditions.

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