Journal of Microbiology

1 "modified immunoblot method"

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Research Support, Non-U.S. Gov't

A Modified Immunoblot Method to Identify Substrates of Protein Kinases: Choong-Min Kang , Wan Jin Jahng , Robert N. Husson , Sang Hee Lee; J. Microbiol. 2011;49(3):499-501. Published online June 30, 2011; DOI: https://doi.org/10.1007/s12275-011-0465-y

Abstract: While protein kinases are key components in multiple cellular processes, efficient identification of cognate in vivo substrates remains challenging. Here we describe a powerful method to screen potential substrates of protein kinases by partial transfer of proteins from a 2D-PAGE gel to a Western blot membrane. This approach allowed precise pinpointing of candidate substrate spots in the 2D gel, and identifying physiological substrates of protein kinases in Mycobacterium tuberculosis.

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