Journal of Microbiology

1 "multiplex nested-polymerase chain reaction (PCR)"

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Detection of Lymphotropic Herpesviruses by Multiplex Polymerase Chain Reaction: Sang-Tae Park , Seung-Han Kim , Dong-Gun Lee , Jung-Hyun Choi , Wan-Shik Shin , Tai - Gyu Ki m , Soon-Young Paik , Chun-Choo Kim; J. Microbiol. 2001;39(3):226-228.

Abstract: Human lymphotropic herpesvirus is known to be a major pathogen associated with various diseases in bone marrow transplantation (BMT) recipients. A multiplex nested-polymerase chain reaction (PCR) method was developed for the simultaneous detection of human lymphotropic herpesviruses, including Ebstein-Barr virus (EBV), cytomegalovirus (CMV), and human herpesvirus 6 variants A and B (HHV6-A, HHV6-B). To demonstrate the usefulness of multiplex PCR for the analysis of clinical samples, peripheral blood mononuclear cells and serum from BMT recipients were analysed. The results showed that a clear detection could be made between EBV, HCMV and HHV-6. This multiplex PCR assay is an efficient and cost-effective approach to the analysis of large numbers of samples to determine the epidemiological importance of EBV, HCMV and HHV-6.

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