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- Rab27b regulates extracellular vesicle production in cells infected with Kaposi’s sarcoma–associated herpesvirus to promote cell survival and persistent infection
- Hyungtaek Jeon , Su-Kyung Kang , Myung-Ju Lee , Changhoon Park , Seung-Min Yoo , Yun Hee Kang , Myung-Shin Lee
- J. Microbiol. 2021;59(5):522-529. Published online April 20, 2021
- DOI: https://doi.org/10.1007/s12275-021-1108-6
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- 4 Web of Science
- 4 Crossref
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Abstract
- Extracellular vesicles (EVs) play a crucial role in cell-to-cell communication. EVs and viruses share several properties related to their structure and the biogenesis machinery in cells. EVs from virus-infected cells play a key role in virus spread and suppression using various loading molecules, such as viral proteins, host proteins, and microRNAs. However, it remains unclear how and why viruses regulate EV production inside host cells. The purpose of this study is to investigate the molecular mechanisms underlying EV production and their roles in Kaposi’s sarcoma-associated herpesvirus (KSHV)-infected cells. Here, we found that KSHV induced EV production in human endothelial cells via Rab- 27b upregulation. The suppression of Rab27b expression in KSHV-infected cells enhanced cell death by increasing autophagic flux and autolysosome formation. Our results indicate that Rab27b regulates EV biogenesis to promote cell survival and persistent viral infection during KSHV infection, thereby providing novel insights into the crucial role of Rab- 27b in the KSHV life cycle.
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Citations
Citations to this article as recorded by
- Engineered small extracellular vesicles as a novel platform to suppress human oncovirus-associated cancers
Iman Owliaee, Mehran khaledian, Armin Khaghani Boroujeni, Ali Shojaeian
Infectious Agents and Cancer.2023;[Epub] CrossRef - HMGB1, a potential regulator of tumor microenvironment in KSHV-infected endothelial cells
Myung-Ju Lee, Joohee Park, Seokjoo Choi, Seung-Min Yoo, Changhoon Park, Hong Seok Kim, Myung-Shin Lee
Frontiers in Microbiology.2023;[Epub] CrossRef - Alpha-2-macroglobulin as a novel diagnostic biomarker for human bladder cancer in urinary extracellular vesicles
Jisu Lee, Hyun Sik Park, Seung Ro Han, Yun Hee Kang, Ji Young Mun, Dong Wook Shin, Hyun-Woo Oh, Yoon-Kyoung Cho, Myung-Shin Lee, Jinsung Park
Frontiers in Oncology.2022;[Epub] CrossRef - Long non-coding RNAs in Sus scrofa ileum under starvation stress
Shu Wang, Yi Jia Ma, Yong Shi Li, Xu Sheng Ge, Chang Lu, Chun Bo Cai, Yang Yang, Yan Zhao, Guo Ming Liang, Xiao Hong Guo, Guo Qing Cao, Bu Gao Li, Peng Fei Gao
Animal Bioscience.2022; 35(7): 975. CrossRef
- Engineered small extracellular vesicles as a novel platform to suppress human oncovirus-associated cancers
- An efficient Agrobacterium-mediated transformation method for aflatoxin generation fungus Aspergillus flavus
- Guomin Han , Qian Shao , Cuiping Li , Kai Zhao , Li Jiang , Jun Fan , Haiyang Jiang , Fang Tao
- J. Microbiol. 2018;56(5):356-364. Published online May 2, 2018
- DOI: https://doi.org/10.1007/s12275-018-7349-3
- 48 View
- 0 Download
- 20 Crossref
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Abstract
- Aspergillus flavus often invade many important corps and produce harmful aflatoxins both in preharvest and during storage stages. The regulation mechanism of aflatoxin biosynthesis in this fungus has not been well explored mainly due to the lack of an efficient transformation method for constructing a genome-wide gene mutant library. This challenge was resolved in this study, where a reliable and efficient Agrobacterium tumefaciens-mediated transformation (ATMT) protocol for A. flavus NRRL 3357 was established. The results showed that removal of multinucleate conidia, to collect a homogenous sample of uninucleate conidia for use as the transformation material, is the key step in this procedure. A. tumefaciens strain AGL-1 harboring the ble gene for zeocin resistance under the control of the gpdA promoter from A. nidulans is suitable for genetic transformation of this fungus. We successfully generated A. flavus transformants with an efficiency of ~ 60 positive transformants per 106 conidia using our protocol. A small-scale insertional mutant library (~ 1,000 mutants) was constructed using this method and the resulting several mutants lacked both production of conidia and aflatoxin biosynthesis capacity. Southern blotting analysis demonstrated that the majority of the transformants contained a single T-DNA insert on the genome. To the best of our knowledge, this is the first report of genetic transformation of A. flavus via ATMT and our protocol provides an effective tool for construction of genome-wide gene mutant libraries for functional analysis of important genes in A. flavus.
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Citations
Citations to this article as recorded by- Agrobacterium tumefaciens-mediated transformation for the genetic modification of the biotechnologically relevant fungus Aspergillus vadensis through synthetic biology
Carolina Ropero-Pérez, Paloma Manzanares, Jose F. Marcos, Sandra Garrigues
Current Research in Biotechnology.2024; 7: 100178. CrossRef - Development of Green Fluorescent Protein-Tagged Strains of Fusarium acuminatum via PEG-Mediated Genetic Transformation
Fangyi Ju, Zhongqiang Qi, Jiajin Tan, Tingting Dai
Microorganisms.2024; 12(12): 2427. CrossRef - An efficient targeted gene deletion approach for Cochliobolus heterostrophus using Agrobacterium tumefaciens-mediated transformation
Jiaying Sun, Rui Yang, Yujia Liu, Zengran Zhou, Jiaqi Jia, Hongming Huang, Shuqin Xiao, Chunsheng Xue
Journal of Microbiological Methods.2024; 216: 106863. CrossRef - Establishment of an Agrobacterium tumefaciens-Mediated Transformation System for Hirsutella sinensis
Lijuan Wu, Xinkun Hu, Shen Yan, Zenglin Wu, Xuzhong Tang, Lei Xie, Yujie Qiu, Rui Li, Ji Chen, Mengliang Tian
Current Issues in Molecular Biology.2024; 46(9): 10618. CrossRef - Role of Flavohemoglobins in the Development and Aflatoxin Biosynthesis of Aspergillus flavus
Xiaoling Zhou, Dongyue Chen, Min Yu, Yuan Jiao, Fang Tao
Journal of Fungi.2024; 10(6): 437. CrossRef - HacA, a key transcription factor for the unfolded protein response, is required for fungal development, aflatoxin biosynthesis and pathogenicity of Aspergillus flavus
Min Yu, Xiaoling Zhou, Dongyue Chen, Yuan Jiao, Guomin Han, Fang Tao
International Journal of Food Microbiology.2024; 417: 110693. CrossRef - Synthetic Biology Tools for Engineering Aspergillus oryzae
Hui Yang, Chaonan Song, Chengwei Liu, Pengchao Wang
Journal of Fungi.2024; 10(1): 34. CrossRef - Construction of Cordycepin High-Production Strain and Optimization of Culture Conditions
Hui Zhang, Ping Chen, Lin Xu, De Xu, Wendi Hu, Yong Cheng, Shengli Yang
Current Microbiology.2023;[Epub] CrossRef - Agrobacterium tumefaciens-mediated transformation of Nigrospora sp. isolated from switchgrass leaves and antagonistic toward plant pathogens
Summi Dutta, Gabriella Houdinet, Gitanjali NandaKafle, Arjun Kafle, Christine V. Hawkes, Kevin Garcia
Journal of Microbiological Methods.2023; 215: 106849. CrossRef - Systematic Characterization of bZIP Transcription Factors Required for Development and Aflatoxin Generation by High-Throughput Gene Knockout in Aspergillus flavus
Qianqian Zhao, Hao Pei, Xiaoling Zhou, Kai Zhao, Min Yu, Guomin Han, Jun Fan, Fang Tao
Journal of Fungi.2022; 8(4): 356. CrossRef - Homologous Expression and Characterization of α-L-rhamnosidase from Aspergillus niger for the Transformation of Flavonoids
Hangyu Ye, Xiaojun Li, Luyuan Li, Yinjun Zhang, Jianyong Zheng
Applied Biochemistry and Biotechnology.2022; 194(8): 3453. CrossRef - Genetic Manipulation and Transformation Methods for Aspergillus spp.
Ye-Eun Son, Hee-Soo Park
Mycobiology.2021; 49(2): 95. CrossRef - Homologous overexpression of genes in Cordyceps militaris improves the production of polysaccharides
Yifeng Wang, Xi Yang, Ping Chen, Shengli Yang, Hui Zhang
Food Research International.2021; 147: 110452. CrossRef - A Novel Site-Specific Integration System for Genetic Modification of Aspergillus flavus
Fang Tao, Kai Zhao, Qianqian Zhao, Fangzhi Xiang, Guomin Han
G3 Genes|Genomes|Genetics.2020; 10(2): 605. CrossRef - Identification of antibiotics for use in selection of the chytrid fungi Batrachochytrium dendrobatidis and Batrachochytrium salamandrivorans
Kristyn A. Robinson, Mallory Dunn, Shane P. Hussey, Lillian K. Fritz-Laylin, Louise A. Rollins-Smith
PLOS ONE.2020; 15(10): e0240480. CrossRef - Aromatic Polyketides from a Symbiotic Strain Aspergillus fumigatus D and Characterization of Their Biosynthetic Gene D8.t287
Yi Hua, Rui Pan, Xuelian Bai, Bin Wei, Jianwei Chen, Hong Wang, Huawei Zhang
Marine Drugs.2020; 18(6): 324. CrossRef - An optimized Agrobacterium tumefaciens-mediated transformation system for random insertional mutagenesis in Fonsecaea monophora
Xing Xiao, Yu Li, JingLin Qin, Ya He, Wenying Cai, Zhiwen Chen, Liyan Xi, Junmin Zhang
Journal of Microbiological Methods.2020; 170: 105838. CrossRef - Genome-wide association study leads to novel genetic insights into resistance to Aspergillus flavus in maize kernels
Guomin Han, Cuiping Li, Fangzhi Xiang, Qianqian Zhao, Yang Zhao, Ronghao Cai, Beijiu Cheng, Xuewen Wang, Fang Tao
BMC Plant Biology.2020;[Epub] CrossRef - The Efficacy of Composite Essential Oils against Aflatoxigenic Fungus Aspergillus flavus in Maize
Fangzhi Xiang, Qianqian Zhao, Kai Zhao, Hao Pei, Fang Tao
Toxins.2020; 12(9): 562. CrossRef - Ethylene and Benzaldehyde Emitted from Postharvest Tomatoes Inhibit Botrytis cinerea via Binding to G-Protein Coupled Receptors and Transmitting with cAMP-Signal Pathway of the Fungus
Yongwen Lin, Hongchun Ruan, Komivi Senyo Akutse, Baochun Lai, Yizhang Lin, Youming Hou, Fenglin Zhong
Journal of Agricultural and Food Chemistry.2019; 67(49): 13706. CrossRef
- Agrobacterium tumefaciens-mediated transformation for the genetic modification of the biotechnologically relevant fungus Aspergillus vadensis through synthetic biology
Research Support, Non-U.S. Gov't
- Identification of seven novel virulence genes from Xanthomonas citri subsp. citri by Tn5-based random mutagenesis
- Xue Song , Jing Guo , Wen-xiu Ma , Zhi-yuan Ji , Li-fang Zou , Gong-you Chen , Hua-song Zou
- J. Microbiol. 2015;53(5):330-336. Published online May 3, 2015
- DOI: https://doi.org/10.1007/s12275-015-4589-3
- 49 View
- 0 Download
- 16 Crossref
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Abstract
- To identify novel virulence genes, a mutant library of Xanthomonas citri subsp. citri 29-1 was produced using EZ-Tn5 transposon and the mutants were inoculated into susceptible grapefruit. Forty mutants with altered virulence phenotypes were identified. Nine of the mutants showed a complete loss of citrus canker induction, and the other 31 mutants resulted in attenuated canker symptoms. Southern blot analysis revealed that each of the mutants carried a single copy of Tn5. The flanking sequence was identified by plasmid rescue and 18 different ORFs were identified in the genome sequence. Of these 18 ORFs, seven had not been previously associated with the virulence of X. citri subsp. citri and were therefore confirmed by complementation analysis. Real-time PCR analysis showed that the seven genes were upregulated when the bacteria were grown in citrus plants, suggesting that the expression of these genes was essential for canker development.
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Citations
Citations to this article as recorded by- Xanthomonas citri subsp. citri type III effector PthA4 directs the dynamical expression of a putative citrus carbohydrate-binding protein gene for canker formation
Xinyu Chen, Huasong Zou, Tao Zhuo, Wei Rou, Wei Wu, Xiaojing Fan
eLife.2024;[Epub] CrossRef - The Methyltransferase HemK Regulates the Virulence and Nutrient Utilization of the Phytopathogenic Bacterium Xanthomonas citri Subsp. citri
Yu Shi, Xiaobei Yang, Xiaoxin Ye, Jiaying Feng, Tianfang Cheng, Xiaofan Zhou, Ding Xiang Liu, Linghui Xu, Junxia Wang
International Journal of Molecular Sciences.2022; 23(7): 3931. CrossRef -
A Comprehensive Overview of the Genes and Functions Required for Lettuce Infection by the Hemibiotrophic Phytopathogen Xanthomonas hortorum pv.
vitians
Lucas Morinière, Laurène Mirabel, Erwan Gueguen, Franck Bertolla, Christopher W. Schadt, Steven Lindow
mSystems.2022;[Epub] CrossRef - Identification of Essential Genes Associated With Prodigiosin Production in Serratia marcescens FZSF02
Xianbo Jia, Fangchen Liu, Ke Zhao, Junjie Lin, Yu Fang, Shouping Cai, Chenqiang Lin, Hui Zhang, Longjun Chen, Jichen Chen
Frontiers in Microbiology.2021;[Epub] CrossRef - An inducible transposon mutagenesis approach for the intracellular human pathogen Chlamydia trachomatis
Colette E. O'Neill, Rachel J. Skilton, Jade Forster, David W. Cleary, Sarah A. Pearson, David J. Lampe, Nicholas R. Thomson, Ian N. Clarke
Wellcome Open Research.2021; 6: 312. CrossRef - The carB Gene of Escherichia coli BL21(DE3) is Associated with Nematicidal Activity against the Root-Knot Nematode Meloidogyne javanica
Yanfei Xia, Shen Li, Guohui Xu, Shanshan Xie, Xueting Liu, Xiaomin Lin, Huijun Wu, Xuewen Gao
Pathogens.2021; 10(2): 222. CrossRef - Comparing bacterial properties in relation to the virulence factors of Xanthomonas citri subsp. citri strains and evaluating resistance of subtribe Citrinae cultivars to the most virulent strain
Hossein Mirzaei-Najafgholi, Milad Aeini, Saeed Tarighi, Morteza Golmohammadi
Journal of Plant Pathology.2021; 103(2): 449. CrossRef - Inhibition of the Citrus Canker Pathogen Using a Photosensitizer Assisted by Sunlight Irradiation
Libin Jiang, Yurong Liu, Xianyuan Xu, Dan Su, Huasong Zou, Jianyong Liu, Cai Yuan, Mingdong Huang
Frontiers in Microbiology.2020;[Epub] CrossRef - Tn5 Transposase Applied in Genomics Research
Niannian Li, Kairang Jin, Yanmin Bai, Haifeng Fu, Lin Liu, Bin Liu
International Journal of Molecular Sciences.2020; 21(21): 8329. CrossRef - A practical random mutagenesis system for Ralstonia solanacearum strains causing bacterial wilt of Pogostemon cablin using Tn5 transposon
Yaqin Wang, Yuyao Zhang, Hua Jin, Zhicheng Deng, Zhuan Li, Yanzhen Mai, Guangwei Li, Hong He
World Journal of Microbiology and Biotechnology.2019;[Epub] CrossRef - Global Regulator PhoP is Necessary for Motility, Biofilm Formation, Exoenzyme Production, and Virulence of Xanthomonas citri Subsp. citri on Citrus Plants
Chudan Wei, Tian Ding, Changqing Chang, Chengpeng Yu, Xingwei Li, Qiongguang Liu
Genes.2019; 10(5): 340. CrossRef - The ColRS-Regulated Membrane Protein Gene XAC1347 Is Involved in Copper Homeostasis and hrp Gene Expression in Xanthomonas citri subsp. citri
Xiaojing Fan, Jing Guo, Yinghui Zhou, Tao Zhuo, Xun Hu, Huasong Zou
Frontiers in Microbiology.2018;[Epub] CrossRef - Salmonella Persistence in Tomatoes Requires a Distinct Set of Metabolic Functions Identified by Transposon Insertion Sequencing
Marcos H. de Moraes, Prerak Desai, Steffen Porwollik, Rocio Canals, Daniel R. Perez, Weiping Chu, Michael McClelland, Max Teplitski, Harold L. Drake
Applied and Environmental Microbiology.2017;[Epub] CrossRef - Identification of New Genes Related to Virulence of <i>Xanthomonas axonopodis</i> Pv. <i>Citri</i> during Citrus Host Interactions
Cristiano B. Ferreira, Leandro M. Moreira, Joice B. Brigati, Lonjoré L. Lima, Jesus A. Ferro, Maria I. T. Ferro, Julio C. F. de Oliveira
Advances in Microbiology.2017; 07(01): 22. CrossRef - Identification of an Extracellular Endoglucanase That Is Required for Full Virulence in Xanthomonas citri subsp. citri
Tian Xia, Yanjiao Li, Dongling Sun, Tao Zhuo, Xiaojing Fan, Huasong Zou, Zonghua Wang
PLOS ONE.2016; 11(3): e0151017. CrossRef - The sigma 54 genes rpoN1 and rpoN2 of Xanthomonas citri subsp. citri play different roles in virulence, nutrient utilization and cell motility
Gibson Kamau Gicharu, Dong-ling SUN, Xun HU, Xiao-jing FAN, Tao ZHUO, Chuan-wan WU, Hua-song ZOU
Journal of Integrative Agriculture.2016; 15(9): 2032. CrossRef
- Xanthomonas citri subsp. citri type III effector PthA4 directs the dynamical expression of a putative citrus carbohydrate-binding protein gene for canker formation
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