MSK
Search
- Page Path
- HOME > Search
Research Support, Non-U.S. Gov't
- Isolation and Characterization of a Mycovirus in Lentinula edodes
- Hyo-Kyoung Won , So-Jung Park , Dong-Kyu Kim , Myeung Ju Shin , Nari Kim , Song-Hee Lee , Young-Chul Kwon , Han Kyu Ko , Hyeon-Su Ro , Hyun-Sook Lee
- J. Microbiol. 2013;51(1):118-122. Published online March 2, 2013
- DOI: https://doi.org/10.1007/s12275-013-2351-2
- 276 View
- 0 Download
- 31 Crossref
-
Abstract
PDF - A mycovirus was isolated from an edible mushroom, Lentinula edodes, that was suffering from a severe epidemic. Fractionation of the diseased cell extract by isopycnic centrifugation with 50% CsCl revealed that the diseased mushroom was infected by Lentinula edodes spherical virus (LeSV), a new spherical virus with a diameter of 55 nm. The particle of LeSV encapsidated the 12 kb RNA genome by a 120 kDa coat protein. BLAST analysis of the partially sequenced LeSV genome showed 95% sequence identity with a putative RNAdependent RNA polymerase (RdRp) gene of the mycovirus HKB, which was previously reported as being a doublestranded RNA (dsRNA) element. In contrast to HKB, the RNA genome in LeSV is encapsidated by the 120 kDa coat protein. To confirm that the LeSV coat protein is encoded by the viral genome, the N-terminal amino acid sequence of the coat protein was determined. The resulting N-terminal amino acid sequence, N-SALDVAPVVPELYFXXLEV-C, was found to be located in the middle of the HKB ORF1, suggesting that the LeSV coat protein was indeed encoded by the virus. To detect LeSV in L. edodes, a primer set targeting the RdRp gene was designed based on the partial sequence of the LeSV genome. RT-PCR analysis showed that 56 of the 84 commercially available dikaryotic cultivars carry LeSV. The transmission pattern of the virus was determined by analysing basidiospores from LeSV-infected and LeSVfree fruiting bodies. Nine out of 10 basidiospores from the LeSV-infected cultivars contained the virus while the spores from the LeSV-free parent were free of LeSV, suggesting that vertical transmission is the primary mode of LeSV propagation.
-
Citations
Citations to this article as recorded by
- Mycoviruses and their ecological impacts on fungi
Mingde Wu, Guoqing Li
Virology.2025; 610: 110562. CrossRef - Characterization of a betaendornavirus isolated from the edible fungus Morchella sextelata
Ruiling Lyu, Junlin Chen, Qing Tang, Du Hai, Tun Wu, Huagang Xiao, Jiatao Xie, Yang Xiao
Archives of Virology.2025;[Epub] CrossRef -
New insights into temperature-impacted mycovirus-fungus interactions regulated by a microRNA in
Lentinula edodes
Chun-Xi Liu, Meng-Pei Guo, Jun-Zhuo Zhou, Yi-Jia Sun, Yin-Bing Bian, Zhang-Yi Xu, W. Allen Miller
Journal of Virology.2025;[Epub] CrossRef - Transmission of Oyster Mushroom Spherical Virus to Progeny via Basidiospores and Horizontally to a New Host Pleurotus floridanus
Yifan Wang, Zhidong Wen, Yaoyao Yang, Xiangting Hu, Zhizhong Song, Haijing Hu, Guoyue Song, Lunhe You, Jianrui Wang, Yu Liu, Xianhao Cheng, Xiaoyan Zhang
International Journal of Molecular Sciences.2024; 25(11): 5677. CrossRef - An Overview of Mycoviral Curing Strategies Used in Evaluating Fungal Host Fitness
Haris Ahmed Khan, Danish Ilyas Baig, Muhammad Faraz Bhatti
Molecular Biotechnology.2023; 65(10): 1547. CrossRef - Horizontal and Vertical Transmission of a Mycovirus Closely Related to the Partitivirus RhsV717 That Confers Hypovirulence in Rhizoctonia solani
Aili Sun, Lianjing Zhao, Yang Sun, Yingrui Chen, Chengyun Li, Wenhan Dong, Genhua Yang
Viruses.2023; 15(10): 2088. CrossRef - The good, the bad and the cryptic: The multifaceted roles of mycoviruses and their potential applications for a sustainable agriculture
Haris Ahmed Khan, Luca Nerva, Muhammad Faraz Bhatti
Virology.2023; 585: 259. CrossRef - Progress in research on diseases of edible fungi and their detection methods: A review
Xiaolong Hou, Changqing Luo, Songshu Chen, Xiuwei Zhang, Junmei Jiang, Zaifu Yang, Fen Wang, Xin Xie
Crop Protection.2023; 174: 106420. CrossRef - Prevalence and diversity of mycoviruses occurring in Chinese Lentinula edodes germplasm resource
Chunxi Liu, Mengpei Guo, Jinjie Wang, Yijia Sun, Yinbing Bian, Zhangyi Xu
Virology.2023; 582: 71. CrossRef - Oyster Mushroom Spherical Virus Crosses the Species Barrier and Is Pathogenic to a New Host Pleurotus pulmonarius
Xiaoyan Zhang, Haijing Hu, Yanxiang Zhao, Yifan Wang, Wenjing Zhang, Lunhe You, Jianrui Wang, Yu Liu, Xianhao Cheng
International Journal of Molecular Sciences.2023; 24(13): 10584. CrossRef - Curing two predominant viruses occurring in Lentinula edodes by chemotherapy and mycelial fragmentation methods
Yijia Sun, Mengpei Guo, Jinjie Wang, Yinbing Bian, Zhangyi Xu
Journal of Virological Methods.2022; 300: 114370. CrossRef - A novel fungal negative-stranded RNA virus related to mymonaviruses in Auricularia heimuer
Xuefei Li, Qingcheng Liu, Shiyu Li, Frederick Leo Sossah, Xuerong Han, Guosheng Zhu, Yu Li, Changtian Li, Yongping Fu
Archives of Virology.2022; 167(11): 2223. CrossRef - Multiplex Detection of Pleurotus ostreatus Mycoviruses
Xiaoyan Zhang, Haijing Hu, Yifan Wang, Junjie Yan, Yu Liu, Jianrui Wang, Xianhao Cheng
Current Issues in Molecular Biology.2022; 44(11): 5778. CrossRef - Diversity of mycoviruses in edible fungi
Yanjing Zhang, Jie Gao, Yu Li
Virus Genes.2022; 58(5): 377. CrossRef - Mycoviral diversity and characteristics of a negative-stranded RNA virus LeNSRV1 in the edible mushroom Lentinula edodes
Mengpei Guo, Guiyu Shen, Jinjie Wang, Mingjie Liu, Yinbing Bian, Zhangyi Xu
Virology.2021; 555: 89. CrossRef - Molecular characterization of a novel fusarivirus infecting the edible fungus Auricularia heimuer
Xuefei Li, Kunpeng Sui, Jiatao Xie, Du Hai, Weiqi Yin, Frederick Leo Sossah, Daohong Jiang, Bing Song, Yu Li
Archives of Virology.2020; 165(11): 2689. CrossRef - Molecular characteristics of a novel ssRNA virus isolated from Auricularia heimuer in China
Xuefei Li, Jiatao Xie, Du Hai, Kunpeng Sui, Weiqi Yin, Frederick Leo Sossah, Daohong Jiang, Bing Song, Yu Li
Archives of Virology.2020; 165(6): 1495. CrossRef - Two novel fungal negative-strand RNA viruses related to mymonaviruses and phenuiviruses in the shiitake mushroom (Lentinula edodes)
Yu-Hsin Lin, Miki Fujita, Sotaro Chiba, Kiwamu Hyodo, Ida Bagus Andika, Nobuhiro Suzuki, Hideki Kondo
Virology.2019; 533: 125. CrossRef - Novel and divergent viruses associated with Australian orchid-fungus symbioses
Jamie W.L. Ong, Hua Li, Krishnapillai Sivasithamparam, Kingsley W. Dixon, Michael G.K. Jones, Stephen J. Wylie
Virus Research.2018; 244: 276. CrossRef - Vertical Transmission of Fusarium circinatum Mitoviruses FcMV1 and FcMV2-2 via Microconidia
Carmen Romeralo, Diana Bezos, Pablo Martínez-Álvarez, Julio Javier Diez
Forests.2018; 9(6): 356. CrossRef - Viruses infecting macrofungi
Ergin Sahin, Ilgaz Akata
VirusDisease.2018; 29(1): 1. CrossRef - Genetic variation and phylogenetic analyses reveal transmission clues of Lentinula edodes partitivirus 1 (LePV1) from the Chinese L. edodes core collection
Jinjie Wang, Mengpei Guo, Yijia Sun, Yinbing Bian, Yan Zhou, Zhangyi Xu
Virus Research.2018; 255: 127. CrossRef - Biological and Molecular Characteristics of a Novel Partitivirus Infecting the Edible Fungus Lentinula edodes
Mengpei Guo, Yinbing Bian, Jinjie Wang, Gangzheng Wang, Xiaolong Ma, Zhangyi Xu
Plant Disease.2017; 101(5): 726. CrossRef - Viral Effects of a dsRNA Mycovirus (PoV-ASI2792) on the Vegetative Growth of the Edible Mushroom Pleurotus ostreatus
Ha-Yeon Song, Hyo-Jin Choi, Hansaem Jeong, Dahye Choi, Dae-Hyuk Kim, Jung-Mi Kim
Mycobiology.2016; 44(4): 283. CrossRef - Molecular characterization of a new monopartite dsRNA mycovirus from mycorrhizal Thelephora terrestris (Ehrh.) and its detection in soil oribatid mites (Acari: Oribatida)
Karel Petrzik, Tatiana Sarkisova, Josef Starý, Igor Koloniuk, Lenka Hrabáková, Olga Kubešová
Virology.2016; 489: 12. CrossRef - Changes in the mycovirus (LeV) titer and viral effect on the vegetative growth of the edible mushroom Lentinula edodes
Jung-Mi Kim, Ha-Yeon Song, Hyo-Jin Choi, Suk-Hyun Yun, Kum-Kang So, Han-Kyu Ko, Dae-Hyuk Kim
Virus Research.2015; 197: 8. CrossRef - Characterization of a Novel Megabirnavirus from Sclerotinia sclerotiorum Reveals Horizontal Gene Transfer from Single-Stranded RNA Virus to Double-Stranded RNA Virus
Minghong Wang, Yong Wang, Xiangzhong Sun, Jiasen Cheng, Yanping Fu, Huiquan Liu, Daohong Jiang, Said A. Ghabrial, Jiatao Xie, A. Simon
Journal of Virology.2015; 89(16): 8567. CrossRef - Genome sequence of a novel mycovirus of Rhizoctonia solani, a plant pathogenic fungus
Jie Zhong, Chuan-Yuan Chen, Bi-Da Gao
Virus Genes.2015; 51(1): 167. CrossRef - Infection of Mycovirus in Imported Lentinula edodes
Song Hee Lee, Seo-Young Kwak, Han Kyu Ko, Hyun-Sook Lee
The Korean Journal of Mycology.2014; 42(1): 64. CrossRef - Diverse double-stranded RNA viruses infecting fungi
Sotaro CHIBA, Nobuhiro SUZUKI
Uirusu.2014; 64(2): 225. CrossRef - Occurrence of dsRNA Mycovirus (LeV-FMRI0339) in the Edible Mushroom Lentinula edodes and Meiotic Stability of LeV-FMRI0339 among Monokaryotic Progeny
Jung-Mi Kim, Suk-Hyun Yun, Seung-Moon Park, Han-Gyu Ko, Dae-Hyuk Kim
The Plant Pathology Journal.2013; 29(4): 460. CrossRef
- Mycoviruses and their ecological impacts on fungi
First
Prev
TOP
