Journal of Microbiology

Journal Articles

The NADPH oxidase AoNoxA in Arthrobotrys oligospora functions as an initial factor in the infection of Caenorhabditis elegans: Xin Li , Ying-Qian Kang , Yan-Lu Luo , Ke-Qin Zhang , Cheng-Gang Zou , Lian-Ming Liang; J. Microbiol. 2017;55(11):885-891. Published online October 27, 2017; DOI: https://doi.org/10.1007/s12275-017-7169-x

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Reactive oxygen species (ROS) produced by NADPH oxidases can serve as signaling molecules to regulate a variety of physiological processes in multi-cellular organisms. In the nematophagous fungus Arthrobotrys oligospora, we found that ROS were produced during conidial germination, hyphal extension, and trap formation in the presence of nematodes. Generation of an AoNoxA knockout strain demonstrated the crucial role of NADPH oxidase in the production of ROS in A. oligospora, with trap formation impaired in the AoNoxA mutant, even in the presence of the nematode host. In addition, the expression of virulence factor serine protease P186 was up-regulated in the wild-type strain, but not in the mutant strain, in the presence of Caenorhabditis elegans. These results indicate that ROS derived from AoNoxA are essential for full virulence of A. oligospora in nematodes.

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AoPrdx2 Regulates Oxidative Stress, Reactive Oxygen Species, Trap Formation, and Secondary Metabolism in Arthrobotrys oligospora
Na Zhao, Meichen Zhu, Qianqian Liu, Yanmei Shen, Shipeng Duan, Lirong Zhu, Jinkui Yang
Journal of Fungi.2024; 10(2): 110. CrossRef
Tools and basic procedures of gene manipulation in nematode-trapping fungi
Shunxian Wang, Xingzhong Liu
Mycology.2023; 14(2): 75. CrossRef
Caenorhabditis elegansLIN‐24, a homolog of bacterial pore‐forming toxin, protects the host from microbial infection
Huijie Zhang, Weirong Zeng, Ming‐Ming Zhao, Jiali Wang, Qiquan Wang, Ting Chen, Yuyan Zhang, Wenhui Lee, Shenghan Chen, Yun Zhang, Xinqiang Lan, Yang Xiang
The FASEB Journal.2023;[Epub] CrossRef
Key processes required for the different stages of fungal carnivory by a nematode-trapping fungus
Hung-Che Lin, Guillermo Vidal-Diez de Ulzurrun, Sheng-An Chen, Ching-Ting Yang, Rebecca J. Tay, Tomoyo Iizuka, Tsung-Yu Huang, Chih-Yen Kuo, A. Pedro Gonçalves, Siou-Ying Lin, Yu-Chu Chang, Jason E. Stajich, Erich M. Schwarz, Yen-Ping Hsueh, Aaron P. Mitc
PLOS Biology.2023; 21(11): e3002400. CrossRef
Recent Advances in Life History Transition with Nematode-Trapping Fungus Arthrobotrys oligospora and Its Application in Sustainable Agriculture
Da Wang, Nan Ma, Wanqin Rao, Ying Zhang
Pathogens.2023; 12(3): 367. CrossRef
Aolatg1 and Aolatg13 Regulate Autophagy and Play Different Roles in Conidiation, Trap Formation, and Pathogenicity in the Nematode-Trapping Fungus Arthrobotrys oligospora
Duanxu Zhou, Yingmei Zhu, Na Bai, Meihua Xie, Ke-Qin Zhang, Jinkui Yang
Frontiers in Cellular and Infection Microbiology.2022;[Epub] CrossRef
Regulatory Mechanism of Trap Formation in the Nematode-Trapping Fungi
Mei-Chen Zhu, Xue-Mei Li, Na Zhao, Le Yang, Ke-Qin Zhang, Jin-Kui Yang
Journal of Fungi.2022; 8(4): 406. CrossRef
Transcriptomic Analysis Reveals That Rho GTPases Regulate Trap Development and Lifestyle Transition of the Nematode-Trapping Fungus Arthrobotrys oligospora
Le Yang, Xuemei Li, Na Bai, Xuewei Yang, Ke-Qin Zhang, Jinkui Yang, Christina A. Cuomo
Microbiology Spectrum.2022;[Epub] CrossRef
Prey sensing and response in a nematode-trapping fungus is governed by the MAPK pheromone response pathway
Sheng-An Chen, Hung-Che Lin, Frank C Schroeder, Yen-Ping Hsueh, A Gladfelter
Genetics.2021;[Epub] CrossRef
Forward genetic screens identified mutants with defects in trap morphogenesis in the nematode-trapping fungus Arthrobotrys oligospora
Tsung-Yu Huang, Yi-Yun Lee, Guillermo Vidal-Diez de Ulzurrun, Yen-Ping Hsueh, J Dunlap
G3 Genes|Genomes|Genetics.2021;[Epub] CrossRef
The NADPH Oxidase A of Verticillium dahliae Is Essential for Pathogenicity, Normal Development, and Stress Tolerance, and It Interacts with Yap1 to Regulate Redox Homeostasis
Vasileios Vangalis, Ioannis A. Papaioannou, Emmanouil A. Markakis, Michael Knop, Milton A. Typas
Journal of Fungi.2021; 7(9): 740. CrossRef
DdaSTE12 is involved in trap formation, ring inflation, conidiation, and vegetative growth in the nematode-trapping fungus Drechslerella dactyloides
Yani Fan, Weiwei Zhang, Yue Chen, Meichun Xiang, Xingzhong Liu
Applied Microbiology and Biotechnology.2021; 105(19): 7379. CrossRef
Azaphilones biosynthesis complements the defence mechanism of Trichoderma guizhouense against oxidative stress
Guan Pang, Tingting Sun, Zhenzhong Yu, Tao Yuan, Wei Liu, Hong Zhu, Qi Gao, Dongqing Yang, Christian P. Kubicek, Jian Zhang, Qirong Shen
Environmental Microbiology.2020; 22(11): 4808. CrossRef
Natural diversity in the predatory behavior facilitates the establishment of a robust model strain for nematode-trapping fungi
Ching-Ting Yang, Guillermo Vidal-Diez de Ulzurrun, A. Pedro Gonçalves, Hung-Che Lin, Ching-Wen Chang, Tsung-Yu Huang, Sheng-An Chen, Cheng-Kuo Lai, Isheng J. Tsai, Frank C. Schroeder, Jason E. Stajich, Yen-Ping Hsueh
Proceedings of the National Academy of Sciences.2020; 117(12): 6762. CrossRef
Guttation capsules containing hydrogen peroxide: an evolutionarily conserved NADPH oxidase gains a role in wars between related fungi
Jian Zhang, Youzhi Miao, Mohammad Javad Rahimi, Hong Zhu, Andrei Steindorff, Sabine Schiessler, Feng Cai, Guan Pang, Komal Chenthamara, Yu Xu, Christian P. Kubicek, Qirong Shen, Irina S. Druzhinina
Environmental Microbiology.2019; 21(8): 2644. CrossRef

GPH1 is involved in glycerol accumulation in the three-dimensional networks of the nematode-trapping fungus Arthrobotrys oligospora: Qin-Yi Wu , Yue-Yan Zhu , Cheng-Gang Zou , Ying-Qian Kang , Lian-Ming Liang; J. Microbiol. 2016;54(11):768-773. Published online October 29, 2016; DOI: https://doi.org/10.1007/s12275-016-6272-8

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Abstract

Turgor is very important for the invasive growth of fungal pathogens. Glycerol, a highly osmotic solvent, is considered to play an important role in turgor generation. The nematophagous fungus Arthrobotrys oligospora mainly lives as a saprophyte. In the presence of nematodes, A. oligospora enters the parasitic stage by forming three-dimensional networks (traps) to capture nematodes. In A. oligospora, we found that glycerol accumulated during nematode-induced trap formation. We demonstrated that deleting gph1, which encodes glycogen phosphorylase, decreased the glycerol content, compared with that of a wild-type strain. Although the number of traps induced by nematodes was not affected in the Δgph1 mutant, the capture rate was lower. Meanwhile, deleting gph1 also affected the growth rate and conidiation capacity of the fungus. These results indicate that glycerol derived from GPH1 is essential for the full virulence of A. oligospora against nematodes.

Citations

Citations to this article as recorded by

Recent Advances in Life History Transition with Nematode-Trapping Fungus Arthrobotrys oligospora and Its Application in Sustainable Agriculture
Da Wang, Nan Ma, Wanqin Rao, Ying Zhang
Pathogens.2023; 12(3): 367. CrossRef
Tools and basic procedures of gene manipulation in nematode-trapping fungi
Shunxian Wang, Xingzhong Liu
Mycology.2023; 14(2): 75. CrossRef
Key processes required for the different stages of fungal carnivory by a nematode-trapping fungus
Hung-Che Lin, Guillermo Vidal-Diez de Ulzurrun, Sheng-An Chen, Ching-Ting Yang, Rebecca J. Tay, Tomoyo Iizuka, Tsung-Yu Huang, Chih-Yen Kuo, A. Pedro Gonçalves, Siou-Ying Lin, Yu-Chu Chang, Jason E. Stajich, Erich M. Schwarz, Yen-Ping Hsueh, Aaron P. Mitc
PLOS Biology.2023; 21(11): e3002400. CrossRef
Aminotransferase SsAro8 Regulates Tryptophan Metabolism Essential for Filamentous Growth of Sugarcane Smut Fungus Sporisorium scitamineum
Guobing Cui, Chengwei Huang, Xinping Bi, Yixu Wang, Kai Yin, Luyuan Zhu, Zide Jiang, Baoshan Chen, Yi Zhen Deng, Slavena Vylkova
Microbiology Spectrum.2022;[Epub] CrossRef
Regulatory Mechanism of Trap Formation in the Nematode-Trapping Fungi
Mei-Chen Zhu, Xue-Mei Li, Na Zhao, Le Yang, Ke-Qin Zhang, Jin-Kui Yang
Journal of Fungi.2022; 8(4): 406. CrossRef
Natural diversity in the predatory behavior facilitates the establishment of a robust model strain for nematode-trapping fungi
Ching-Ting Yang, Guillermo Vidal-Diez de Ulzurrun, A. Pedro Gonçalves, Hung-Che Lin, Ching-Wen Chang, Tsung-Yu Huang, Sheng-An Chen, Cheng-Kuo Lai, Isheng J. Tsai, Frank C. Schroeder, Jason E. Stajich, Yen-Ping Hsueh
Proceedings of the National Academy of Sciences.2020; 117(12): 6762. CrossRef

Research Support, Non-U.S. Gov't

Diversity and Metal Tolerance of Nematode-Trapping Fungi in Pb-Polluted Soils: Ming-He Mo , Wei-Min Chen , Hao-Ran Yang , Ke-Qin Zhang; J. Microbiol. 2008;46(1):16-22.; DOI: https://doi.org/10.1007/s12275-007-0174-8

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Abstract: The diversity of nematode-trapping fungi (NTF) in two lead (Pb) mines in Yunnan Province, China was investigated in 2004. In total, 20 species belonging to five genera were identified from 500 samples collected at the Lanping and the Huize mines. Pb concentrations ranged from 216~7,150 mg/kg for the former and 132~13,380 mg/kg for the latter, respectively. The fungi were divided into five groups based on different trapping mechanisms. The trapping-net producer group contained the largest number of species, with nine. Two predators, Dactylellina ellipsosporum and Arthrobotrys oligospora, were found at frequencies of 32.85% and 15.41%, respectively. The diversity indexes of NTF were positively correlated with Pb pollution levels in both the Lanping Mine (r=0.66) and the Huize Mine (r=0.72), suggesting that the distribution of NTF was not negatively affected by Pb contamination. For most strains of a given species, there was no significant difference (P>0.01) in the Pb tolerance between the strains isolated from habitats with low or high Pb concentrations. However, Pb toxicity exerted adverse effects on trap formation and predacious capability of fungi. We discuss the possible metal tolerance mechanisms and their relationships to the survival strategy of NTF in Pb-polluted environments.

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