MSK
Search
- Page Path
- HOME > Search
Journal Article
- Performance of nested multiplex PCR assay targeting MTP40 and IS6110 gene sequences for the diagnosis of tubercular lymphadenitis
- Pallavi Sinha , Pradyot Prakash , Shashikant C.U. Patne , Shampa Anupurba , Sweety Gupta , G. N. Srivastava
- J. Microbiol. 2017;55(1):63-67. Published online December 30, 2016
- DOI: https://doi.org/10.1007/s12275-017-6127-y
- 45 View
- 0 Download
- 5 Crossref
-
Abstract
- The conventional methods for diagnosis of tubercular lymphadenitis (TBLN) such as - fine needle aspiration cytology, Ziehl-Neelsen staining and culture have limitations of low sensitivity and/or specificity. So, it becomes essential to develop a rapid, sensitive, and specific method for an early diagnosis of TBLN. Therefore, the present study was conducted to evaluate nested multiplex polymerase chain reaction (nMPCR) targeting MTP40 and IS6110 gene sequences of Mycobacterium tuberculosis and Mycobacterium tuberculosis complex, respectively in 48 successive patients of TBLN and 20 random patients with non-tubercular lymph node lesions. Out of the 48 cases of TBLN, 14 (29.2%) were found to be positive by Ziehl-Neelsen staining, 15 (31.2%) were positive by culture and 43 (89.6%) cases were positive after first round of PCR while 48 (100%) cases were positive by nMPCR assay. The sensitivity and specificity of nMPCR was found to be 100% for the diagnosis of TBLN. The results thus obtained indicate that nMPCR assay is a highly sensitive and specific tool for the diagnosis of TBLN.
-
Citations
Citations to this article as recorded by
- Diagnosis of tuberculous lymphadenitis by molecular and immunological tools
Nitin Kumar, Anish Khan, Sanjit Boora, Neha Chadha, Nisha Khan, Puneet Raina, Rajesh Gupta, Raj Singh, Samander Kaushik
Medicine in Microecology.2024; 22: 100116. CrossRef - Evaluating the Sensitivity of Different Molecular Techniques for Detecting Mycobacterium tuberculosis Complex in Patients with Pulmonary Infection
Hassan A. Hemeg, Hamzah O. Albulushi, Hani A. Ozbak, Hamza M. Ali, Emad K. Alahmadi, Yahya A. Almutawif, Sari T. Alhuofie, Rana A. Alaeq, Areej A. Alhazmi, Mustafa A. Najim, Ahmed M. Hanafy
Polish Journal of Microbiology.2023; 72(4): 421. CrossRef - The Relevance of Genomic Epidemiology for Control of Tuberculosis in West Africa
Prince Asare, Adwoa Asante-Poku, Stephen Osei-Wusu, Isaac Darko Otchere, Dorothy Yeboah-Manu
Frontiers in Public Health.2021;[Epub] CrossRef - Two target genes based multiple cross displacement amplification combined with a lateral flow biosensor for the detection of Mycobacterium tuberculosis complex
Junfei Huang, Ziyu Xiao, Xinggui Yang, Xu Chen, Xiaojuan Wang, Yijiang Chen, Wenlin Zheng, Wei Chen, Huijuan Chen, Shijun Li
BMC Microbiology.2021;[Epub] CrossRef - Duplex PCR for Detection of Aleutian Disease Virus from Biological and Environmental Samples
Marek Kowalczyk, Andrzej Jakubczak, Magdalena Gryzińska
Acta Veterinaria.2019; 69(4): 402. CrossRef
- Diagnosis of tuberculous lymphadenitis by molecular and immunological tools
First
Prev
TOP
