As an ancient seed plant, cycads are one of the few gymnosperms
that develop a root symbiosis with cyanobacteria,
which has allowed cycads to cope with harsh geologic and
climatic conditions during the evolutionary process. However,
the endophytic microbes in cycad roots remain poorly identified.
In this study, using next-generation sequencing techniques,
we investigated the microbial diversity and composition
of both the coralloid and regular roots of Cycas bifida
(Dyer) K.D. Hill. Highly diverse endophytic communities were
observed in both the coralloid and regular roots. Of the associated
bacteria, the top five families were the Nostocaceae,
Sinobacteraceae, Bradyrhizobiaceae, Bacillaceae, and Hyphomicrobiaceae.
The Nectriaceae, Trichocomaceae, and Incertae
sedis were the predominant fungal families in all root samples.
A significant difference in the endophytic bacterial community
was detected between coralloid roots and regular
roots, but no difference was observed between the fungal communities
in the two root types. Cyanobacteria were more dominant
in coralloid roots than in regular roots. The divergence
of cycad root structures and the modified physiological
processes may have contributed to the abundance of cyanobionts
in coralloid roots. Consequently, the colonization of
cyanobacteria inhibits the assemblage of other endophytes.
Our results contribute to an understanding of the species diversity
and composition of the cycad-endophyte microbiome
and provide an abbreviated list of potential ecological roles
of the core microbes present.
Citations
Citations to this article as recorded by
Dictyophora indusiata and Bacillus aryabhattai improve sugarcane yield by endogenously associating with the root and regulating flavonoid metabolism Mingzheng Duan, Xiang Li, Xiaojian Wu, Shengfeng Long, Hairong Huang, Yijie Li, Qi-Huai Liu, Guanghu Zhu, Bin Feng, Sunqian Qin, Changning Li, Hai Yang, Jie Qin, Zhendong Chen, Zeping Wang Frontiers in Plant Science.2024;[Epub] CrossRef
Phylometagenomics of cycad coralloid roots reveals shared symbiotic signals Edder D. Bustos-Diaz, Arely Cruz-Perez, Diego Garfias-Gallegos, Paul M. D'Agostino, Michelle M. Gehringer, Angelica Cibrian-Jaramillo, Francisco Barona-Gomez
Microbial Genomics
.2024;[Epub] CrossRef
Cycadales' defense against insect and mammalian herbivory: Do endophytic fungi have to say something? - A hypothesis Yogesh Joshi, Pooja Bansal, Arjun Lal Yadav Fungal Biology Reviews.2024; 50: 100393. CrossRef
Insight into saffron associated microbiota from different origins and explore the endophytes for enhancement of bioactive compounds Sirui Xu, Liang Hong, Tong Wu, Xinting Liu, Zihan Ding, Li Liu, Qingsong Shao, Ying Zheng, Bingcong Xing Food Chemistry.2024; 456: 140006. CrossRef
Leaf Endophytes Relationship with Host Metabolome Expression in Tropical Gymnosperms Adriel M. Sierra, Omayra Meléndez, Rita Bethancourt, Ariadna Bethancourt, Lilisbeth Rodríguez-Castro, Christian A. López, Brian E. Sedio, Kristin Saltonstall, Juan Carlos Villarreal A. Journal of Chemical Ecology.2024; 50(11): 815. CrossRef
Diversity of symbiotic cyanobacteria in cycad coralloid roots using a short-read rbcL-X amplicon Adriel M. Sierra, Sandrine Toupin, Marta Alonso-García, Juan Carlos Villarreal A. Symbiosis.2024; 92(2): 271. CrossRef
Emerging nitrogen-fixing cyanobacteria for sustainable cotton cultivation Lucía Jiménez-Ríos, Alejandro Torrado, José Luis González-Pimentel, Macarena Iniesta-Pallarés, Fernando P. Molina-Heredia, Vicente Mariscal, Consolación Álvarez Science of The Total Environment.2024; 924: 171533. CrossRef
The improvement of kinsenoside in wild-imitated cultivation Anoectochilus roxburghii associated with endophytic community Ying Zheng, Lihong Li, Xinting Liu, Sirui Xu, Xutong Sun, Zili Zhang, Haipeng Guo, Qingsong Shao Industrial Crops and Products.2024; 208: 117896. CrossRef
Cyanobacteria as a critical reservoir of the environmental antimicrobial resistome V. J. Timms, K. A. Hassan, L. A. Pearson, B. A. Neilan Environmental Microbiology.2023; 25(11): 2266. CrossRef
Effects of antibiotics on the endophyte and phyllosphere bacterial communities of lotus from above and below surface water in a typical shallow lake Ling Zhang, Junhong Bai, Yujia Zhai, Kegang Zhang, Yaqi Wang, Rong Xiao, Milko A. Jorquera Plant Physiology and Biochemistry.2023; 201: 107812. CrossRef
Core Endophytic Bacteria and Their Roles in the Coralloid Roots of Cultivated Cycas revoluta (Cycadaceae) Jiating Liu, Haiyan Xu, Zhaochun Wang, Jian Liu, Xun Gong Microorganisms.2023; 11(9): 2364. CrossRef
Changes in rice rhizosphere and bulk soil bacterial communities in the Doñana wetlands at different growth stages Macarena Iniesta-Pallarés, Manuel Brenes-Álvarez, Ana V. Lasa, Manuel Fernández-López, Consolación Álvarez, Fernando P. Molina-Heredia, Vicente Mariscal Applied Soil Ecology.2023; 190: 105013. CrossRef
Niche differentiation shapes the bacterial diversity and composition of apple Yimei Huang, Xiaofen Chai, Xiaona Wang, Beibei Gao, Hui Li, Zhenhai Han, Xuefeng Xu, Xinzhong Zhang, Ting Wu, Yi Wang Horticultural Plant Journal.2023; 9(1): 35. CrossRef
Core Microbiome and Microbial Community Structure in Coralloid Roots of Cycas in Ex Situ Collection of Kunming Botanical Garden in China Zhaochun Wang, Jian Liu, Haiyan Xu, Jiating Liu, Zhiwei Zhao, Xun Gong Microorganisms.2023; 11(9): 2144. CrossRef
Combined intensive management of fertilization, tillage, and organic material mulching regulate soil bacterial communities and functional capacities by altering soil potassium and pH in a Moso bamboo forest Ying Zheng, Xinzhu Liu, Yanjiang Cai, Qingsong Shao, Wei Zhu, Xinchun Lin Frontiers in Microbiology.2022;[Epub] CrossRef
Full-Length 16S rRNA and ITS Gene Sequencing Revealed Rich Microbial Flora in Roots of Cycas spp. in China Melissa H Pecundo, Aimee Caye G Chang, Tao Chen, Thomas Edison E dela Cruz, Hai Ren, Nan Li Evolutionary Bioinformatics.2021;[Epub] CrossRef
Differences in lipid homeostasis and membrane lipid unsaturation confer differential tolerance to low temperatures in two Cycas species Yanling Zheng, Yongqiong Yang, Meng Wang, Shijun Hu, Jianrong Wu, Zhixiang Yu BMC Plant Biology.2021;[Epub] CrossRef
Diversity, Phylogeny and Antagonistic Activity of Fungal Endophytes Associated with Endemic Species of Cycas (Cycadales) in China Melissa H. Pecundo, Thomas Edison E. dela Cruz, Tao Chen, Kin Israel Notarte, Hai Ren, Nan Li Journal of Fungi.2021; 7(7): 572. CrossRef
The complete chloroplast genome of Cycas bifida, an extremely small population protected species Deng Zhang, Yanqiang Cao, Zhaocen Lu Mitochondrial DNA Part B.2021; 6(10): 2960. CrossRef
The Predominance of Proteobacteria and Cyanobacteria in the Cycas dolichophylla Coralloid Roots Revealed by 16S rRNA Metabarcoding Y. Zheng, T.-Y. Chiang, Ch-Li Huang, X.-Y. Feng, K. Yrjälä, X. Gong Microbiology.2021; 90(6): 805. CrossRef
Biological control of Ralstonia solanacearum (Smith), the causal pathogen of bacterial wilt disease by using Pantoea spp Kamal A. M. Abo-Elyousr, Sabry A. Hassan Egyptian Journal of Biological Pest Control.2021;[Epub] CrossRef
The diversity and community structure of symbiotic cyanobacteria in hornworts inferred from long‐read amplicon sequencing Jessica M. Nelson, Duncan A. Hauser, Fay‐Wei Li American Journal of Botany.2021; 108(9): 1731. CrossRef
Biological potential of bioactive metabolites derived from fungal endophytes associated with medicinal plants Priyanka Kumari Keshri, Nilesh Rai, Ashish Verma, Swapnil C. Kamble, Suvakanta Barik, Pradeep Mishra, Santosh Kumar Singh, Prafull Salvi, Vibhav Gautam Mycological Progress.2021; 20(5): 577. CrossRef
Endophytes from Gnetum gnemon L. can protect seedlings against the infection of phytopathogenic bacterium Ralstonia solanacearum as well as promote plant growth in tomato Heena Agarwal, Bhaskar Dowarah, Pooja Moni Baruah, Kuntala Sarma Bordoloi, Debasish B. Krishnatreya, Niraj Agarwala Microbiological Research.2020; 238: 126503. CrossRef
Exploring cycad foliage as an archive of the isotopic composition of atmospheric nitrogen Michael A. Kipp, Eva E. Stüeken, Michelle M. Gehringer, Kim Sterelny, John K. Scott, Paul I. Forster, Caroline A. E. Strömberg, Roger Buick Geobiology.2020; 18(2): 152. CrossRef
Contrasting bacteriome of the hornwort Leiosporoceros dussii in two nearby sites with emphasis on the hornwort-cyanobacterial symbiosis R. Bouchard, G. Peñaloza-Bojacá, S. Toupin, Y. Guadalupe, J. Gudiño, N. Salazar Allen, F. W. Li, J. C. Villarreal A. Symbiosis.2020; 81(1): 39. CrossRef
Specialized bacteriome uncovered in the coralloid roots of the epiphytic gymnosperm, Zamia pseudoparasitica Philip Bell‐Doyon, Jérôme Laroche, Kristin Saltonstall, Juan Carlos Villarreal Aguilar Environmental DNA.2020; 2(4): 418. CrossRef
Niche Specialization and Functional Overlap of Bamboo Leaf and Root Microbiota Ying Zheng, Xinchun Lin Frontiers in Microbiology.2020;[Epub] CrossRef
Chemical Element Concentrations of Cycad Leaves: Do We Know Enough? Benjamin E. Deloso, Murukesan V. Krishnapillai, Ulysses F. Ferreras, Anders J. Lindström, Michael Calonje, Thomas E. Marler Horticulturae.2020; 6(4): 85. CrossRef
Niche differentiation rather than biogeography shapes the diversity and composition of microbiome of Cycas panzhihuaensis Ying Zheng, Xun Gong Microbiome.2019;[Epub] CrossRef
Cycad Coralloid Roots Contain Bacterial Communities Including Cyanobacteria andCaulobacterspp. That Encode Niche-Specific Biosynthetic Gene Clusters Karina Gutiérrez-García, Edder D Bustos-Díaz, José Antonio Corona-Gómez, Hilda E Ramos-Aboites, Nelly Sélem-Mojica, Pablo Cruz-Morales, Miguel A Pérez-Farrera, Francisco Barona-Gómez, Angélica Cibrián-Jaramillo, Tal Dagan Genome Biology and Evolution.2019; 11(1): 319. CrossRef
Unlocking a high bacterial diversity in the coralloid root microbiome from the cycad genus Dioon Pablo de Jesús Suárez-Moo, Andrew P. Vovides, M. Patrick Griffith, Francisco Barona-Gómez, Angélica Cibrián-Jaramillo, Sabrina Sarrocco PLOS ONE.2019; 14(2): e0211271. CrossRef