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[MINIREVIEW] Interdependence between iron acquisition and biofilm formation in Pseudomonas aeruginosa
Donghoon Kang , Natalia V. Kirienko
J. Microbiol. 2018;56(7):449-457.   Published online June 14, 2018
DOI: https://doi.org/10.1007/s12275-018-8114-3
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AbstractAbstract
Bacterial biofilms remain a persistent threat to human healthcare due to their role in the development of antimicrobial resistance. To combat multi-drug resistant pathogens, it is crucial to enhance our understanding of not only the regulation of biofilm formation, but also its contribution to bacterial virulence. Iron acquisition lies at the crux of these two subjects. In this review, we discuss the role of iron acquisition in biofilm formation and how hosts impede this mechanism to defend against pathogens. We also discuss recent findings that suggest that biofilm formation can also have the reciprocal effect, influencing siderophore production and iron sequestration.

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Research Support, Non-U.S. Gov't
Sporulation of Several Biocontrol Fungi as Affected by Carbon and Nitrogen Sources in a Two-Stage Cultivation System
Li Gao , Xingzhong Liu
J. Microbiol. 2010;48(6):767-770.   Published online January 9, 2011
DOI: https://doi.org/10.1007/s12275-010-0049-2
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AbstractAbstract
The development of fungal biopesticides requires the efficient production of large numbers spores or other propagules. The current study used published information concerning carbon concentrations and C:N ratios to evaluate the effects of carbon and nitrogen sources on sporulation of Paecilomyces lilacinus (IPC-P and M- 14) and Metarhizium anisopliae (SQZ-1-21 and RS-4-1) in a two-stage cultivation system. For P. lilacinus IPCP, the optimal sporulation medium contained urea as the nitrogen source, dextrin as the carbon source at 1 g/L, a C:N ratio of 5:1, with ZnSO4·7H2O at 10 mg/L and CaCl2 at 3 g/L. The optimal sporulation medium for P. lilacinus M-14 contained soy peptone as the nitrogen source and maltose as the carbon source at 2 g/L, a C:N ratio of 10:1, with ZnSO4·7H2O at 250 mg/L, CuSO4·5H2O at 10 mg/L, H3BO4 at 5 mg/L, and Na2MoO4·2H2O at 5 mg/L. The optimum sporulation medium for M. anisopliae SQZ-1-21 contained urea as the nitrogen source, sucrose as the carbon source at 16 g/ L, a C:N ratio of 80:1, with ZnSO4·7H2O at 50 mg/L, CuSO4·5H2O at 50 mg/L, H3BO4 at 5 mg/L, and MnSO4·H2O at 10 mg/L. The optimum sporulation medium for M. anisopliae RS-4-1 contained soy peptone as the nitrogen source, sucrose as the carbon source at 4 g/L, a C:N ratio of 5:1, with ZnSO4·7H2O at 50 mg/L and H3BO4 at 50 mg/L. All sporulation media contained 17 g/L agar. While these results were empirically derived, they provide a first step toward low-cost mass production of these biocontrol agents.
Journal Article
S5 Lipase : An Organic Solvent Tolerant Enzyme
Raja Noor Zaliha Raja Abdul Rahman , Syarul Nataqain Baharum , Abu Bakar Salleh , Mahiran Basri
J. Microbiol. 2006;44(6):583-590.
DOI: https://doi.org/2470 [pii]
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AbstractAbstract
In this study, an organic solvent tolerant bacterial strain was isolated. This strain was identified as Pseudomonas sp. strain S5, and was shown to degrade BTEX (Benzene, Toluene, Ethyl-Benzene, and Xylene). Strain S5 generates an organic solvent-tolerant lipase in the late logarithmic phase of growth. Maximum lipase production was exhibited when peptone was utilized as the sole nitrogen source. Addition of any of the selected carbon sources to the medium resulted in a significant reduction of enzyme production. Lower lipase generation was noted when an inorganic nitrogen source was used as the sole nitrogen source. This bacterium hydrolyzed all tested triglycerides and the highest levels of production <br><br>were observed when olive oil was used as a natural triglyceride. Basal medium <br><br>containing Tween 60 enhanced lipase production to the most significant degree. The <br><br>absence of magnesium ions (Mg2+) in the basal medium was also shown to stimulate lipase production. Meanwhile, an alkaline earth metal ion, Na+, was found to stimulate the production of S5 lipase.
Factors affecting pheromone induction of schizosaccharomyces pomba and isolation of pheromone induction mutants
Jun, Jai Hyun , Kim, Young Min , Lee, Joo Hun , Chung, In Kwon , Kim, Dae Myung
J. Microbiol. 1998;36(3):196-202.
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AbstractAbstract
The mating pheromones of Schizosaccharomyces pombe are induced by nutritional starvation. However, this nutritional signaling pathway is largely unknown. For a complete understanding of pheromone induction, we examined the environmental factors affecting the induction afer cells were transferred to a nitrogen-starved medium. It appeared that the induction of mfm2 transcription was affected by the general environmental stress including incubation time, incubation temperature, and the growth phase of the cells. We identified 7 pheromone induction mutants by screening temperature sensitive mutant bank. Three of these mutants showed elongated cell shapes and one mutant exhibited swollen cell morphology in permissive culture, suggesting that their cell cycles were also impaired. Characterization of the pheromone induction mutants may elucidate the components required in nutritional signaling pathway leading to pheromone induction.

Journal of Microbiology : Journal of Microbiology
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