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Inhibitory Effect of Methyl Gallate and Gallic Acid on Oral Bacteria
Mi-Sun Kang , Jong-Suk Oh , In-Chol Kang , Suk-Jin Hong , Choong-Ho Choi
J. Microbiol. 2008;46(6):744-750.   Published online December 24, 2008
DOI: https://doi.org/10.1007/s12275-008-0235-7
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AbstractAbstract
This study examined the ability of methyl gallate (MG) and gallic acid (GA), the main compounds of gallotannins in Galla Rhois, to inhibit the proliferation of oral bacterial and the in vitro formation of Streptococcus mutans biofilms. The antimicrobial activities of these compounds were evaluated in vitro using the broth microdilution method and a beaker-wire test. Both MG and GA had inhibitory effects on the growth of cariogenic (MIC<8 mg/ml) and periodontopathic bacteria (MIC=1 mg/ml). Moreover, these compounds significantly inhibited the in vitro formation of S. mutans biofilms (MG, 1 mg/ml; GA, 4 mg/ml; P<0.05). MG was more effective in inhibiting bacterial growth and the formation of S. mutans biofilm than GA. In conclusion, MG and GA can inhibit the growth of oral pathogens and S. mutans biofilm formation, and may be used to prevent the formation of oral biofilms.

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Research Support, Non-U.S. Gov't
Bacterial Diversity in the Human Saliva from Different Ages
Jung-Gyu Kang , Seong Hwan Kim , Tae-Young Ahn
J. Microbiol. 2006;44(5):572-576.
DOI: https://doi.org/2438 [pii]
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AbstractAbstract
To obtain primary idea on oral bacterium species that are generally present in periodotally healthy Koreans, the oral bacterial flora in the saliva of four periodontally healthy Koreans at different ages (5, 32, 35, 65) was investigated in this study. For this investigation, 16SrRNA gene clone libraries were generated from the saliva of the four healthy Koreans, and 50 clones were randomly selected from each saliva clone library and sequenced. Totally, 37 different kinds of bacterial 16S rRNA gene sequences were identified based on sequence homology search through GenBank database. The 37 kinds of saliva clone sequences were classified to 14 genera and 2 uncultured and 1 unidentified bacteria. Among the 14 identified genera, Streptococcus, Prevotella, and Veillonella were common genera, and Streptococcus was dominant genus that accounted for 7 different species. Among the seven Streptococcus species, S. salivarius appeared as the most common species. More numbers of species belonging to the genera Streptococcus and Prevotella was present in saliva from ages 32 and 35. While saliva from ages 5 and 65 showed more numbers of species belonging to the genera Rothia, including potential pathogenic species. Overall, saliva of a young child and a senior showed higher bacterial diversity than that of young adults.

Journal of Microbiology : Journal of Microbiology
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